Objective To study the effect of purslane decoction on oxidative stress products and soluble protein of senile cataract patients tears.Methods 120 cases of cataract patients (160 eyes) were selected, according to different drugs were randomly assigned to two groups.The control group were treated with Imidacloprid sinnock sodium drops eye drops, 1 drop each time, 4 times per day,the patients in the experimental group on the basis of the control group with Portulaca oleracea decoction 100 mL, for three consecutive months.SOD, MDA, soluble protein level and visual improvement were compared after the end of treatment.Results Compared with control group, the visual acuity of the experimental group was higher(P<0.05), MDA was lower, SOD was higher(P<0.05), soluble protein content was higher(P<0.05).Conclusion Portulaca oleracea decoction can apparently improve the cataract patients with tear of SOD, MDA, soluble protein level and improve visual acuity.

Objective To study the impact of physical stimulus on periosteal chondroplasia with an in- tention of reconstructing cartilage tissue that has a biomechanical function and can be created either entirely in vivo or in vitro and subsequently implanted into the patient.Methods A series of biomechanical test methods were designed in order to investigate the mechanical properties of engineered cartilage under a variety of mechanical stress stimulations.The periosteal explants were harvested from proximal medial tibias of New Zealand rabbits and secured onto poly-?-caprolactone(PCL)scaffolds.The composites(periosteum/PCL)were suspended in the spinner flask bioreactor and the spinner flask was located on the stirring machine.The stem cells were stimulated by fluid shear stress under magnetic bar stirring.Results In gross observation,the thickness and length of neo-cartilage with shear force stimulation were significantly greater than those without shear force.The direction of elongation was parallel with fluid flow direction,regardless of composite orientation.Secondary,two different zones based on various cell morphology and distribution were clearly observed.The thickness of the superficial zone was greater in samples exposed to high shear force compared with low shear force.The superficial zone protein and lubricant were found in the engineered cartilage under shear stress stimulation.Conclusion The results reveal that shear stress can not only influence proliferation and differentiation of stem cells,but also change chondrocyte morphology and increase extracellular matrix production.

Some types of bacteria swim through rotating their flagella. The swimming mechanism of bacteria during flagella bundling and tumble process is analyzed. The effects of body rotation and flagellum′s polymorphic transitions on bundling processes and the wall effect phenomenon are also discussed. Finally, based on dynamics similarity, a new microrobot module is put forward to further studying the flagella swimming phenomena. The research would be very helpful for constructing the bionic swimming robots under the low Reynolds number.

Objective To discuss the clinical significance of the diffuse calcified distribution in diagnosis of benign and malignant breast lesions .Methods 379 patients with different benign and malignant breast lesions confirmed by surgery underwent digital X‐ray mammography .The morphology ,distribution ,number ,diameter ,concentration and density of calcification in lesions ,the maximum range of the calcified area and other accompanied manifestations in benign and malignant breast lesions were analyzed .Results As for the morphology of calcification ,tiny polymorphic calcification was found in 58 .5% of malignant lesions ,meanwhile ,dot‐like one was found in 49 .3% of benign lesions .Fine linear calcification or branched linear one occurred in malignant lesions ,however ,round one occurred in benign lesions .As for the calcification distribution ,regional distribution was found in 43 .9% of malignant lesions , meanwhile the clustered distribution was found in 58 .4% of benign lesions .And all lobar or segmental distribution was seen in malig‐nant lesions .As for the calcification diameter ,the calcification with the diameter less than 0 .5 mm occurred in 71 .6% of malignant lesions ,however ,that with diameter more than 1 .0 cm occurred in 69 .3% of benign lesions .As for the concentration of calcification , lesions with calcification more than 25 were 71 .8% of malignant ones ,whereas those with calcification of 15-25 were 58 .4% of be‐nign ones .As for calcified density ,uneven calcification occurred in 94 .2% of malignant lesions ;however the even one occurred in 63 .4% of benign lesions .The maximum diameter of calcification ranged from 40 mm to 80 mm was found in 59 .0% of malignant lesions , whereas that ranged from 0 mm to 40 mm was in 77 .2% of benign lesions .Conclusion Statistical differences have been found in the morphology ,distribution ,diameter ,concentration ,density and maximum diameter of calcification between the breast benign and ma‐lignant lesions .

AIM:To observe the clinical effect of femtosecond laser assisted penetrating keratoplasty.
METHODS: Twenty-four cases ( 24 eyes ) with corneal lesions were performed with femtosecond laser assisted penetrating keratoplasty. Preoperative and postoperative endothelial cell density and visual quality were compared.RESULTS: One week after operation, corneal grafts were clear in 21 eyes (87. 5%), mild cloudy in 3 eyes (12.5%);visual acuity ≥0. 5 in 18 eyes (75. 0%), 0. 2 ~0.4 in 6 eyes ( 25. 0%). After 3mo the mean corneal astigmatism was 2. 16±0. 21D ( range 2. 25 ~ 3. 09D). Compared to conventional penetrating keratoplasty which mean corneal astigmatism was average 3. 67±0. 38D after operation, there was significant difference between two groups ( P< 0. 05 ). There were significant differences between preoperative and postoperative visual acuity and astigmatism (both P<0. 05).
CONCLUSION: Femtosecond laser assisted penetrating corneal transplantation operation can improve patient's visual quality. And compared to traditional penetrating keratoplasty astigmatism decreased significantly, incision can be made in individual shape more precisely and neatly.

OBJECTIVE:To prepare the compound chlorhexidine acetate ear drops for treating anaerobic and aerobic infections of antrum auris METHODS:The compound chlorhexidine acetate ear drops was prepared with mixed solvent of glycerin,alcohol and distilled water The contents of two main ingredients were determined by dual-wavelength isobestic point spectrophotometry and the stability of preparation was examined RESULTS:The average recovery of metronidazole was 99 34%(RSD=0 57%,n=6) and that of chlorhexidine acetate was 101 17%(RSD=0 88%,n=6) CONCLUSION:The new preparation is rational in formula,simple in quality control and good in stability and has good prospects in development

OBJECTIVE: To investigate the genetic polymorphisms of 21 autosomal STR loci of Fujian Han population and evaluate the forensic application value of GlobalFiler Express kit. METHODS: Amplified with GlobalFiler Express kit, DNA samples were obtained from 741 unrelated individuals of Fujian Han population. The population genetics parameters of 21 autosomal STR loci were calculated. RESULTS: The 21 autosomal STR loci were found to be no deviation from Hardy-Weinberg equilibration (P > 0.05) and relatively abundant in high polymorphism. Heterozygosity ranged from 0.589 to 0.914, power of discrimination ranged from 0.754 to 0.992, polymorphic information content ranged from 0.520 to 0.940, and power of exclusion ranged from 0.278 to 0.825. The SE33 locus was the highest degree in polymorphism. CONCLUSION The 21 STR loci of GlobalFiler Express kit have high value in discrimination power and can be useful in personal identification and paternity test in Fujian Han population.
Asian People/genetics* ; DNA ; Genetics, Population ; Heterozygote ; Humans ; Microsatellite Repeats ; Paternity ; Polymorphism, Genetic

Most chemical medicines have polymorphs. The difference of medicine polymorphs in physicochemical properties directly affects the stability, efficacy, and safety of solid medicine products. Polymorphs is incomparably important to pharmaceutical chemistry, manufacturing, and control. Meantime polymorphs is a key factor for the quality of high-end drug and formulations. Polymorph prediction technology can effectively guide screening of trial experiments, and reduce the risk of missing stable crystal form in the traditional experiment. Polymorph prediction technology was firstly based on theoretical calculations such as quantum mechanics and computational chemistry, and then was developed by the key technology of machine learning using the artificial intelligence. Nowadays, the popular trend is to combine the advantages of theoretical calculation and machine learning to jointly predict crystal structure. Recently, predicting medicine polymorphs has still been a challenging problem. It is expected to learn from and integrate existing technologies to predict medicine polymorphs more accurately and efficiently.

Objective To explore the role of glutamate in inducing cortical neuron damage in newborn rats.Methods The model of damage induced by glutamate was established on cultured cortical neurons in newborn rats with primary cultivation technique.To evaluate the severity of neuron injury, the changes of morphology were observed by inverted microscopy, the cell viability and rate of LDH releasing from neuron were detected by MTT assay and biochemical method,respectively;the rate of neuronal apoptosis was measured by flow cytometer system.Results Under the inverted microscopy, neurons showed obvious toxic damage in glutamate treatment group. Compared with controls,the cell viability significantly decreased (t=4.58 P

Objective To investigate the role and intracellular signal transduction mechanism in the injury of renal tubular cells induced by postasphyxial-serum in neonate.Methods Human renal proximal tubular cell(HK-2 cell) was used as target cell. The experiment was designed as:control group, asphyxia group ,and pyrrolidine dithiocarbamate (PDTC)blocking group. The attacking concentration of serum was 20%, and the apoptosis rate of HK-2 cells was detected by flow cytometer.Results Compared with controls[(13.3?1.70)%],after being stimulated with postasphyxial-serum, the apoptosis rate of HK-2 cells of asphyxia group [(46.73?3.68)%] and PDTC blocking group [(31.19?2.79)%]were significantly increased(P