Animals ; Calcium ; metabolism ; Cell Line ; Cricetinae ; Cricetulus ; Dose-Response Relationship, Drug ; Fibroblasts ; drug effects ; metabolism ; Vehicle Emissions

OBJECTIVETo study the therapeutic effects of posterolateral depression fractures of the tibial plateau through a modified anterolateral approach.

METHODSFrom February 2011 to January 2012,13 patients with posterolateral depression fractures of the tibial plateau were treated through a modified anterolateral approach. There were 8 males and 5 females, ranging in age from 28 to 59 years old (49.2 years old on average). Data from patients were collected retrospectively as follows: X-ray, time of fracture healing and the complications of fracture healing. The patients were evaluated both clinically and radiologically according to the Rasmussen score system.

RESULTSAll the patients were followed up, and the duration ranged from 6 to 18 months (mean 13.7 months). All the patients got bony union. The average radiographic bony union time was 15.1 weeks (ranged, 11 to 17 weeks). No case of secondary articular depression was found. No complications such as malunion or joint stiffness were found. But 1 patient had superficial infection and 1 patient had common peroneal nerve injury. According to the Rasmussen score system,the mean radiological score was 16.50 ± 0.67 (ranged, 13 to 18), and the mean functional score was 25.20 ± 2.21 (ranged, 13 to 30). The mean range of knee motion was (125.3 ± 9.3)° (ranged, 0° to 135°).

CONCLUSIONTreatment of depression fractures of posterolateral tibial plateau with a modified anterolateral approach is a safe method with effective exposure, due to its stable fixation and relatively good outcome with minimal soft-tissue complications. It is regarded as an ideal procedure for depression fractures of posterolateral tibial plateau.

Adult ; Female ; Fracture Fixation, Internal ; methods ; Fracture Healing ; Humans ; Male ; Middle Aged ; Retrospective Studies ; Tibial Fractures ; surgery

Head and Neck Neoplasms ; surgery ; Hemangioma, Cavernous ; surgery ; Humans ; Infant ; Thoracic Neoplasms ; surgery

OBJECTIVETo study the genetic polymorphism of twelve short tandem repeat loci of Y chromosome in Chaoshan Han populations.

METHODSTwelve Y short tandem repeat loci, of which the template DNAs were extracted from blood samples of 121 unrelated Chaoshan Han individuals, were amplified by using the PowerPlex Y system. The PCR products were genotyped with ABI PRISM 3100 genetic analyzer.

RESULTSFrom the loci DYS19, DYS437, DYS389I, DYS389II, DYS438, DYS439, DYS393, DYS391, DYS390, and DYS392, 4-7 alleles were checked out; from the loci DYS385, 35 alleles were observed. The overall observed haplotype diversity reached 0.9976. DYS391 was the locus with the lowest gene diversity (0.4445), while DYS385 had the highest gene diversity (0.9525).

CONCLUSIONThe 12 Y-STR loci are highly polymorphic and suitable to forensic casework, population genetics and anthropology studies.

China ; Chromosomes, Human, Y ; genetics ; Gene Frequency ; Genetic Variation ; Genotype ; Haplotypes ; genetics ; Humans ; Male ; Microsatellite Repeats ; genetics ; Polymorphism, Genetic ; genetics

OBJECTIVECurrent study was conducted to investigate and compare the impact of temperature and pH on the activities of amylase, protease and lipase in alimentary tract of Whitmania pigra.

METHODThe responses of amylase, protease, and lipase activities were determined over a wide range of temperatures (7-52 degrees C) and pH gradient (2.2-11.2).

RESULTThe highest lipase activity was found under 37 degrees C, pH 8.2, and the highest amylase activity was detected under 37 degrees C, pH 5.2, while protease activity peaked at 42 degrees C, pH 3.2 or pH 9.2.

CONCLUSIONThe optimal temperature in alimentary tract of Wh. pigra for lipase and amylase was 37 degrees C, and the responding temperature for protease was 42 degrees C. The optimal pH value in alimentary tract of Wh. pigra for lipase and amylase was pH 8.2 and pH 5.2, respectively. While pH 3.2 or 9.2 seems to be both favorable for high protease activity.

Amylases ; chemistry ; metabolism ; Animals ; Digestive System ; chemistry ; enzymology ; Enzyme Stability ; Hydrogen-Ion Concentration ; Leeches ; chemistry ; enzymology ; Lipase ; chemistry ; metabolism ; Peptide Hydrolases ; chemistry ; metabolism ; Temperature

AIMTo study the chemical constituents from Hedysarum multijugum.

METHODSThe compounds were separated by chromatography methods, their structures were identified by spectral analysis.

RESULTSSix compounds were isolated and identified as beta-sitosterol (1), 7-hydroxy-4'-methoxy isoflavone (2), betulic acid (3), 1,7-dihydroxy-3, 9-dimethoxy pterocarpene (4), 5, 7-dihydroxy-8-C-prenyl-4'-methoxy isoflavone (5) and 5, 7-dihydroxy-4'-methoxy isoflavone (6).

CONCLUSIONCompound 4 is a new compound and the others were obtained from the plant for the first time.

Benzofurans ; chemistry ; isolation & purification ; Benzopyrans ; chemistry ; isolation & purification ; Fabaceae ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Sitosterols ; chemistry ; isolation & purification ; Triterpenes ; chemistry ; isolation & purification

OBJECTIVETo search for a better method for frozen shoulder due to cold damp.

METHODSSixty cases of frozen shoulder were randomly divided into an acupuncture-moxibustion group (32 cases) and an acupuncture group (28 cases). Acupuncture was applied mainly at Jianyu (LI 15), Jianliao (TE 14) and Jianzhen (SI 9) in either group. In acupuncture-moxibustion group, moxibustion on tender points was supplemented.

RESULTSIn acupuncture-moxibustion group, 7 cases were cured, 15 cases markedly effective, 9 cases improved and 1 case failed. The cured and markedly effective rate was 68.8% and the effective rate was 96.9%. In acupuncture group, 2 cases were cured, 9 cases markedly effective, 13 cases improved and 4 cases failed. The cured and markedly effective rate was 39.3% and the effective rate was 85.7%. The cured and markedly effective rate in acupuncture-moxibustion group was superior to that in acupuncture group (P < 0.05) and there was no significant difference in the effective rate statistically between two groups (P > 0.05).

CONCLUSIONThe clinical efficacy of acupuncture combined with moxibustion on tender points is superior to that of simple acupuncture on frozen shoulder due to cold damp.

Acupuncture Therapy ; methods ; Aged ; Bursitis ; therapy ; Cold Temperature ; Female ; Humans ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Moxibustion ; methods

Objective To explore the clinical efficacy of focused ultrasound for patients with white lesions of the vulva,as well as its safety and feasibility.Methods Clinical data of 941 patients with white lesions of the vulva treated with focused ultrasound from June 2003 to December 2005 were retrospectively reviewed.The mean age of the patients was 40.8 years(18-70 years)and the median course of the disease was 6.2 years(3 months-45 years).Meanwhile,pathological diagnosis was performed in all the patients before treatment,in which 498 cases were squamous hyperplasia,342 cases were lichen sclerosus and 101 cases were lichen sclerosus with squamous hyperplasia.Patients were followed up and therapeutic effects of focused ultrasound was evaluated at 6 and 12 months after the treatment,respectively.The symptoms of pruritus in the vulva and the changes in the color and elasticity of the vulvar lesions were observed.Results Of all the patients,900 were followed up after the treatment,and the ratio of effectiveness was 94.9%.Only 46 patients(5.1%)had no response to the therapy.Of the effective patients,434 cases were completely cured(48.2%),and 420 cases were improved(46.7%).Pruritus of vulva recurred in 101 patients (11.2%)one year after treatment;however,these patients still had a response to the second or third treatment.Conclusions Focused ultrasound therapy is a highly effective instrument in treatment of white lesions of the vulva.It can not only relieve the symptoms of itching,but is also helpful in recovering the color and elasticity of the vulva.

Objective To explore the physical and mental health status on the ‘left behind ’ students in Anhui province and the related influential factors.Methods Stratified cluster sampling method was used to investigate the 682 out of 3421 students from 7 middle schools in both south and north Anhui province,using multidimensional sub-health questionnaire of adolescents (MSQA) and mental health test (MHT),for assessment of the general condition,physical and mental health status.Results 14.22％ of the ‘left behind’ students were in sub-health condition compared to 11.28％ among the normal students,and the mental sub-health status represented 13.64％ for the former and 10.84％ for the latter.In addition,the ‘left behind’ students appeared less content with their life than those of staying with their parents (P＜0.05).MHT scores in the subjects were positively correlated with the scores on MSQA and showed significant difference (P＜0.01).Results from the multiple linear regression analysis showed that the mental health condition among the left behind students was in linear regression relationship with the decreased physical activity (β =1.456,P=0.001 ),emotional problems (β =1.096,P=0.000 ) and problems of social adaptation (β=1.011,P=0.006).The status of physical sub-health in the Anhui province showed that there was no significant difference in each of the study group (P＞0.05).Conclusion Decreased physical activity and emotion aporia as well as difficult social adaptation were major factors leading to degenerated mental health levels in the‘left-behind’ adolescents.The findings suggested that the mental health status could be improved inthis population through better management on their body-health condition.

OBJECTIVETo investigate the relationship between the changes of amino acids contents in hippocampus of rats and electromagnetic pulse (EMP) exposure.

METHODSRats were decapitated and hippocampus were removed after EMP (6 x 10(4) V/m, rise time 20 ns, pulse width 30 micro s, 5 pulses in 2 minutes) irradiation, and contents of amino acids were detected with high performance liquid chromatograpy (HPLC).

RESULTSThe contents of aspartic acid (Asp) and glutamic acid (Glu) increased significantly 0, 3, 6 h after irradiation. The peak values of Asp [(17.25 +/- 1.63) pmol/ micro l] and Glu [(13.67 +/- 0.95) pmol/ micro l] were higher than those of control [(10.56 +/- 1.50), (6.94 +/- 1.10) pmol/ micro l respectively, P < 0.05]. Then both decreased gradually and reached the normal level 24 - 48 h after irradiation. The contents of glycine (Gly), taurine (Tau) and gamma-aminobutyric acid (GABA) also rose after exposure, the peak value of them [(4.51 +/- 0.60), (29.85 +/- 2.70), (5.14 +/- 0.73) pmol/ micro l respectively] were higher than those of control group [(2.18 +/- 0.31), (9.88 +/- 1.47), (2.84 +/- 0.67) pmol/ micro l, P < 0.05], then recovered 48 h after irradiation. The value of Glu/GABA increased immediately after exposure (3.45 +/- 0.25, P < 0.05), then decreased 24 h (1.62 +/- 0.23, P < 0.05) and recovered 48 h after exposure.

CONCLUSIONThe toxic effect of excess excitatory amino acids may be partly responsible for the early retardation (within 24 h) of learning of rats.

Amino Acids ; analysis ; Animals ; Chromatography, High Pressure Liquid ; methods ; Glutamic Acid ; analysis ; Hippocampus ; metabolism ; radiation effects ; Male ; Radiation ; Random Allocation ; Rats ; Rats, Wistar ; Time Factors