Objective To study the role of cyclcoxygenase-2 in the growth and development of the endome-trial lesions. Methods The expression of cycloogxygenase-2 (COX-2) were tested by immunohistocbemistry. Compar-ison of endometrial cancer COX-2 expression was made within each group at different stages,for different pathological classification. Another groups comparison of COX-2 expression was made. Results ( 1 ) COX-2 in endometrial cancer in the different expression of other differences and differences were significant ( P<0.05 ). Ⅲ mainly for the period between the stage and the other had a significant difference( P<0.05 ). (2) COX-2 in endometrial cancer,hyperplasia and normal expression of the differences between endometriosis, the difference was significant ( P<0.05 ). (3)COX-2 in endometrium cancer different pathology graduation difference:G1(high idiophase) < G2(idiophase) < G3(low id-iophase), but does not have the difference after the comparison in statistics ( P>0.05 ). (4) The expression of COX-2 in simple hyperplasia and complex hyperplasia was statistically significant different ( P<0.05 ). Conclusion That COX-2 in the development of endometrial lesions play an important role, it could be inferred of COX-2 inhibitors for endometrial cancer treatment or treatment of postoperative the timing of the loss surgery patients is feasible.

Objective To study the relationship between survival rate and pathogen,subtype,stage,delivered methods of 55 patients with severe hepatitis during late period of pregnancy.Methods Clinical data of 55 cases with severe hepatitis during late period of pregnancy was analyzed retrospectively.Results The incidence of hepatitis B was highest in patients with severe hepatitis during late period of pregnancy (84.5%).In subtypes,the incidence rates of chronic severe hepatitis,acute severe hepatitis and subacute severe hepatitis were 83.6%,5.5%,10.9% respectively(P

The traditional medical treasures of zhouyi and qianjin have much in common in the aspects of logic approach,recognition characteristics,values conception,theory construction,and category system,which demonstrate that the two doctrines are closely related both geographically and historically. Bearing the same cultural genes and value orientation,sharing similar constructions of logic approaches and recognition characteristics,the two doctrines contain a great deal of wisdom in recognition and practice.

Objective To investigate the infection type,clinical features and prognosis in the infections complication patients with severe hepatitis.Methods 72 severe hepatitis patients with infections complication were analyzed retrospectively.Results The infection occurrence rate was 62.5% in the 72 severe hepatitis patients.Mortality rate was 75.56% in the patients with infections complication,and it was significantly higher than those without infections complication(29.63%).The mortality rate was 72.5% in the patients with multiple organ infections.The different bacterium infection could lead to different mortality rate,K.peneumoniae(69.89%),E.coli(58.12%),Staphylococcus aureaus(55.56%),Staphylococcus epidermidis(59.90%).The mortality rate was higher in the patients with lower sera albumin and higher serum bilirubin level.Conclusions The patients with severe hepatitis have bad outcome with infections complication.The prognosis is correlated with the dysfunction severity of liver and pathogenic bacteria.

Objective To investigate the role of TRAF2 in AP-1 signaling pathway of human B cells.Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion wild type TRAF2(YFP-WT-TRAF2) or YFP fusion dominant-negative TRAF2(DN-TRAF2),or transfected with shRNA-TRAF2 and control shRNA plasmid.After incubation overnight,cells were either sorted with flowcytometry or screened by antibiotics G418.Activation of AP-1 pathway,including phosphorylation of ERK,JNK and P38,as well as nuclear translocation of AP-1 subunits were detected by western blot and ELISA.Results Overexpression of WT-TRAF2 selectively induced activition of MAPK by phosphorylation of ERK and P38,and further induced nuclear translocation of C-FOS.Moreover,both overexpression of DN-TRAF2 and transfection of shRNA-TRAF2 inhibited phosphorylation of ERK and P38,and nuclear translocation of C-FOS.Conclusion TRAF2 selectively activates some kinases in CD40 mediated AP-1 signaling pathway,and plays an important role in AP-1 activation.

Objective To analyze the main biochemical indexes in the patients with type 2 diabetes mellitus (T2DM) and complications.Methods A total of 112 cases of patients with T2DM in the Zigong Municipal Third People's Hospital were selected from March 2015 to March 2016 and divided into the T2DM without complications group (61 cases) and T2DM with complications group (51 cases) according to whether having complications.Contemporaneous 55 volunteers undergoing physical examination were selected as the control group.The levels of insulin,fasting blood glucose (FBG),D-dimer (D-D) and serum lipids (HDL,LDL,TC and TG) were compared among three groups.Results The levels of FBG,D-D,TC,TG and LDL in the T2DM without complications group and T2DM with complications group were higher than those in the control group,while the levels of insulin and HDL were lower than those in the control group,the differences were statistically significant (P<0.05);moreover,except HDL,the levels of insulin,FBG,D-D,LDL,TC and TG had statistical difference between the T2DM with complications group and T2DM without complications group (P<0.05).Conclusion Regularly detecting the biochemical indexes of blood glucose and lipids in diabetic patients is an important mean to preventing and treating diabetes mellitus and its complications.

With chinese pharmaceutical companies in the rapid development, there are many factors restricting their development, this article through consulting relevant literature, data and investing, analyzing the shortage of the pharmaceutical enterprises at the present stage in China and the factors of restricting their development, so as to put forward the solution to the current situation of pharmaceutical enterprises in China and the related measures, make more long-term and healthy development of pharmaceutical enterprises in China.

Objective To investigate the effects of different concentrations of isoflurane on neuronal apoptosis in hippocampus and cerebral cortex in the aged rats. Methods Ninety female pathogen-free SD rats 22-24 months old weighing 497-593 g were randomly divided into 3 groups: group Ⅰ oxygen group inhaled air enriched with 40% O2,groupⅡ and Ⅲ were anesthetized with 1.2% and 1.8% isoflurane for 3 h respectively. Morris water maze was used to assess the cognitive function. The animals were killed at 24 and 72 h and on the 7th day after emergence from anesthesia and their brains were removed. Neuronal apoptosis in hippocampus and cerebral cortex was detected by TUNEL. Results The average escape latency was significantly longer on the 2nd and 3rd day after isoflurane anesthesia in isoflurane group than in oxygen group (P < 0.05 ). The neuronal apoptesis rate was significantly higher in the 2 isoflurane group than in oxygen group (P < 0.05 or 0.01 ). Conclusion Isoflurane anesthesia can result in transient cognitive dysfunction, and the mechanism may be involved in apoptosis in cerebral cortex in the aged rats.

Objective To study the curative efficacy of mometasone furoate aqueous nasal spray combined with loratadine in the treatment of pediatric allergic rhinitis, and its effects on IL-10, IL-17 in serum.Methods 126 patients of pediatric allergic rhinitis who were admitted in our hospital from May 2011 to September 2013 were selected as research objects and randomly divided into treatment group and control group, 63 patients in each group.The control group were treated with loratadine, while the treatment group were treated with mometasone furoate aqueous nasal spray in combination with loratadine.The curative efficacy, scores of clinical symptoms and nasal examination, curing time, medication time and serum IL-10, IL-17 levels were compared between the two groups.Results The total therapeutic efficacy ratio in the treatment group was 98.41%, which was statistically higher than that of 80.95% in the control group (P<0.05).5 weeks after the treatment, scores of sneezing, runny nose, nasal congestion, nasal itching, eye itching and nasal examination in the treatment group were statistically lower than those in the control group (P<0.05).The curing time and medication time in the observation group were statistically shorter than those in the control group ( P <0.05 ) .The inflammatory factors, in comparison with the control group after treatment, the treatment group had statistically higher level of serum IL-10 and lower level of serum IL-17 ( P <0.05 ) . Conclusion Mometasone furoate aqueous nasal spray in combination with loratadine is effective for pediatric allergic rhinitis with a high therapeutic efficacy ratio.And it could significantly improve clinical symptoms, shorten treatment time, increase level of IL-10 and reduce level of IL-17.

Objective To observe the clinical efficacy of triple needling and centro-square needling in treating hemifacial spasm. Method Sixty-eight patients with facial spasm were randomized into a treatment group and a control group, 34 in each group. The treatment group was intervened by centro-square needling at topical Ashi points and triple needling at Yifeng (TE 17) on the affected side; the control group was intervened by regular acupuncture. The two groups were treated once a day, 10 times as a treatment course, for 3 courses in total. Cohen Albert grading scale was adopted to evaluate the spasm intensity before and after the intervention, and the clinical efficacies were then compared.Result After intervention, there was a significant difference in comparing the spasm intensity between the two groups (P<0.05). The significant improvement rate was 88.2% in the treatment group versus 50.0% in the control group, and the difference was statistically significant (P<0.05).Conclusion Triple needling plus centro-square needling is an effective method in treating hemifacial spasm. This method uses less points, but it produces a high efficacy, strong pertinence, and is easy-to-operate.