To compare posterior choroidal thickness in high myopia amblyopia eyes at different points to high myopia and normal eyes of Chinese children and investigate the relationships between choroidal thickness, axial length and age.METHODS: Fifty Chinese children (65 eyes) with age 4~15 years ( mean 9. 91 ± 3. 41 years) were recruited. By atropine optometry they were divided into high myopia amblyopia group ( 24 eyes ) , high myopia group ( 19 eyes ) , and normal group ( 22 eyes ) . Choroidal scans were obtained for all eyes using enhanced depth imaging spectral-domain optical coherence tomography ( EDl-OCT) . Subfoveal choroidal thickness (SFCT), macular thinkness, choroidal thickness and retinal thickness at 0. 5, 1. 0, 1. 5, 2.0, 2.5, 3.0mm superior (S, 12:00 position), temporal ( T, 9:00 position) , inferior ( l, 6:00 position) , nasal ( N, 3:00 position) were measured. Meanwhile, axial lengths of all eyes were measured by A-Scan. RESULTS: Compared high myopia group and emmetropia group, SFCT and the thickness of choroids on each position were thinnest in high myopic amblyopia group, with statistically significant differences (P<0. 05). There was a significant negative correlation between SFCT and axial length in high myopic amblyopia group (r=-0. 531, R2 =0. 282, F=7. 476, P=0. 013), with no relative in age (r=-0. 292, R2=0. 085, F=2. 044, P=0. 167).CONCLUSlON: The choroidal thickness thinning in high myopic amblyopia shows a negative correlation with axial length.

·AlM:To investigate the retinal thickness change of high myopia amblyopic children, so as to discuss the relationships between the retinal thickness of central fovea of macula and the factors of axis oculi and age. · METHODS:Thirty-nine children ( 65 eyes ) with the average age of ( 9.91 3.41 ) years were recruited.All eyes were ruled out the pathological changes of fundus diseases and front section. After a tropine optometry, they were divided into three groups: high myopia amblyopic group ( 24 eyes ) , high myopia group ( 19 eyes) and normal group ( 22 eyes ) .Retinal scans were obtained for all eyes using Heidelberg optical coherence tomography ( OCT ) . Subfoveal macular thickness, retinal thickness at 0.5mm, 1.0mm, 1.5mm, 2.0mm, 2.5mm, 3.0mm superior ( S, 12∶00 position), temporal (T, 9∶00 position), inferior (l, 6∶00 position) and nasal (N, 3∶00 position) from the fovea were measured and axial length was also surveyed by A -ultrasound. Statistical analyses were performed to evaluate retinal thickness at each location and to correlate subfoveal macular thickness with axial length and age.
·RESULTS:The average subfoveal macular thinkness of the high myopia amblyopic group was thinner than high myopia group but thicker than normal group.There was no statistical difference between three groups (P>0.05). Retinal thickness inferior to the fovea at 0.5mm temporal and superior to the fovea in the high myopia amblyopic group at 1.0mm temporal were both thinner than normal group which had statistically significant ( P <0.05 ). Retinal thickness on nasal, superior, temporal, and inferior at 1.5mm, 2.0mm, 2.5mm, 3.0mm from the fovea were measured, high myopia amblyopic group were the thinnest in the three groups, and there was statistically significant between three groups ( P<0.05). There was no correlation between the average subfoveal macular thickness and axial length, age in high myopia amblyopic group.
· CONCLUSlON:There are significant abnormalities of macula retinal structure in high myopia amblyopic children.

AIM: To research the peripapillary retinal nerve fiber layer ( RNFL ) thickness change in high myopia amblyopic children and to discuss the relationships among RNFL thickness, axial length and age.
METHODS:Thirty-five Chinese children (59 eyes) with a mean age of ( 9. 59 ±2. 90 ) years were recruited. All eyes were ruled out the pathological changes of fundus diseases and front section. By atropine optometry after they were divided into: high myopia amblyopia group (22 eyes), high myopia group (15 eyes), normal group (22 eyes) . RNFL scans were obtained for all eyes using optical coherence tomography and axial length was also surveyed by A - ultrasound. Statistical analyses were performed to evaluate RNFL thickness at each location with axial length and age.
RESULTS:The peripapillary RNFL thickness in temporal of high myopia amblyopia group was thinner than that in
high myopia group, and thicker than that in normal group. The peripapillary RNFL thickness in nasal, superior, inferior and the average thickness of high myopia amblyopia group were thinner than those in high myopia and normal gruops. The peripapillary RNFL thickness in inferior and average thickness of high myopia amblyopia group were significantly thinner than those of high myopia (P<0. 05). The peripapillary RNFL thickness in nasal, superior, inferior and the average thickness of high myopia amblyopia group were significantly thinner than those of normal (P<0. 01). The peripapillary RNFL thickness in temporal of high myopia group was significantly thicker, and in nasal, superior, inferior and the average thickness were significantly thinner than those of normal (P<0. 05). The thickness of peripapillary RNFL in inferior showed a negative correlation with axial length in high myopia amblyopia group (R=0. 474, R2=0. 225, F=4. 933, P=0. 040). The thickness of peripapillary RNFL in superior showed a negative correlation with axial length in high myopia group (R=0. 642, R2=0. 412, F=9. 104,P=0. 010). These were no correlation between the peripapillary RNFL thickness and age in high myopia amblyopia, myopia amblyopia and normal.
CONCLUSION:There are significant abnormalities of retinal structure in high myopia amblyopia.

Action Potentials ; drug effects ; Animals ; CA1 Region, Hippocampal ; cytology ; Drug Antagonism ; Male ; Neurons ; drug effects ; Pentylenetetrazole ; pharmacology ; Propofol ; pharmacology ; Rats ; Rats, Wistar ; Synaptic Transmission ; drug effects

To explore the diagnostic values of miRNA141 and miRNA375 for prostate cancer.The expressions of miRNA141 and miRNA375 in serum samples from 30 prostate cancer (PCA) patients,40 benign prostatic hyperplasia patients and 50 normal controls were detected by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).The expression of miRNA141 in tissue samples from prostate cancer was 13.4±7.2 folds of that from benign prostatic hyperplasia while the expression of miRNA375 in tissue samples from prostate cancer was 28.4 ± 10.3 folds of that from benign prostatic hyperplasia.The differential expression of miRNA141 and miRNA375 in sera of prostate cancer suggested that miRNA141 and miRNA375 might be potential tumor markers in clinical practice.

Objective To investigate the reason of persisting positive rapid plasma reagin (RPR) in serofast syphilis patients, and to provide reference for clinical treatment and prognosis.Methods A total of 33 serofast patients and 23 healthy controls were enrolled in this study.The percentages and absolute counts of CD3+, CD4+, CD8+ T lymphocytes and natural killer (NK) cells were detected by flow cytometry.The comparison of two groups was analyzed by independent sample t test, and the correlation between change of lymphocyte subgroups and RPR titer in serofast syphilis patients was analyzed by bivariate linear correlation method.Results Compared with healthy controls, the percentages of CD3+, CD8+ T lymphocytes in serofast syphilis group were both increased significantly (75.75±5.76)% vs (68.37±5.80)%, (t=4.69, P<0.01);(27.34±7.02)% vs (24.33±1.95)%, (t=2.34, P=0.025), while both the percentage and absolute count of NK cells were significantly decreased (7.32±4.48)% vs (14.87±6.26)%, (t=5.269, P<0.01);(136.2±83.4)/μL vs (298.8±166.9)/μL, (t=4.311, P<0.01).RPR titer of the patients was negatively correlated with both percentage and absolute count of CD4+ T lymphocytes (r=-0.476 and-0.515, respectively, both P<0.01), and it was positively correlated of CD8+ lymphocytes (r=0.588 and 0.305, P<0.01 and P=0.804).Conclusion The imbalance of immune response of lymphocyte subsets observed in serofast syphilis may explain the RPR titers change.

Objective To explore the features of high-frequency ultrasonography and contrast-enhanced ultrasonography (CEUS) of papillary thyroid microcarcinoma (PTMC).Methods The CEUS data and ultrasound data of 147 PTMCS which were reconfirmed by pathology were analyzed retrospectively,and the CEUS and ultrasonic characteristics of them were summarized.Results Among 147 nodules,144 (97.9％) nodules were hypoechoic,and 3 nodules were isoechoic.Vague edge was found in 136(92.5％) PTMCs,and 126(85.7％) PTMCs were irregular in shape.Totally 92(62.6％) PTMCs were A/T ＞ 1,microcalcifications were found in 81 (55.1％) PTMCs.Besides,26(74.2％) PTMCs were found microcalcification in 35 PTMCs combined with Hashimoto's thyroiditis (HT),while 55 (49.1％) PTMCs were found microcalcification in 112 PTMCs combined with HT.There were significant differences between them (P ＜ 0.05).The blood distribution of 129 (87.8％) nodules was type Ⅱ.The contrast-enhanced pattern of 147 (100.0％) PTMCs showed in-homogeneous enhancement in 144 (97.9％) nodules,hypoenhancement in 136(92.5％) nodules,and all the nodules without amicula.Conclusions The typical PTMCs are hypoechoic,irregular shapeand vague edge,usually were found as A/T ＞ 1,microcalcification,and type Ⅱ blood distribution.With the method of contrast-enhanced ultrasonography,these nodules usually without amicula showed inhomogeneous and hypoenhancement.The incidence of microcalcification is more common when patients with Hashimoto's disease coexisting PTMC.

Objective To investigate the clinical value of the probe melting curve analysis‐based PCR assay for the detection of three types of αT .Methods A total of 149 blood and prenatal archival DNA samples (6 of which were amniotic fluid samples)with three knownαT genes ,which included 63 carriers with Hb CS ,22 cases with Hb QS ,43 individuals with Hb WS and 1 double heter‐ozygote with Hb CS and Hb WS) as well as 20 samples with normalα‐globin gene sequence that had been confirmed by RBD com‐bined with DNA sequencing were selected to test the specificity of probe melting curve analysis by blind analysis .Results The probe melting curve analysis accurately detected 100 of the DNA samples previously characterized by S RBD combined with DNA sequencing .Conclusion Probe melting curve analysis‐based PCR assay for the detection ofαT is featured with rapidity and accuracy and can be applied to clinical and prenatal diagnosis .

BACKGROUND: It is commonly believed that hyperbaric oxygen (HBO)plays a neuroprotective role in the prevention and treatment of cerebral infarction (CI), however hyperbaric oxygen therapy (HBOT)-induced CI was also reported.OBJECTIVE: To investigate the risk factors of HBOT-induced CI.DESIGN: Case analysis with patients as subjects.SETTING: At the Department of Hyperbaric Oxygen of Guangzhou General Hospital of Guangzhou Military Command Area of Chinese PLA.PARTICIPANTS: Totally 192 inpatients receiving HBOT were selected from the Department of Hyperbaric Oxygen of Guangzhou General Hospital Guangzhou Military Command Area of Chinese PLA between December 1996 and March 1998, including 127 males and 65 females with age ranging from 9 to 78 years old. Totally 6 patients, 3 males and 3 females, suffered from CI during HBOT, with the age from 51 to 76 yeas.METHODS: The background factors of 192 patients who HBOT METHODS and 6 cases with induced CI were analyzed.MAIN OUTCOME MEASURES: Symptoms, signs and skull CT or MRI.RESULTS: Data of 192 patients were dealt with intentionally and all were statistically analyzed without miss. ① The incidence of CI during HBOT was 0.3% (6/192). ② The risk factors of HBOT-induced CI included: age of ≥ 60 years, hypertension, hyperlipidemia, primary CI or cerebral hemorrhage, blood hyperviscosity and diabetes, and the incidence would greatly increased in those who has 4 or more than 4 risk factors, but seldom with less than 4 risk factors.CONCLUSION: The cluster level of risk factors was closely related with the incidence of HBOT-induced CI.

Obiective To introduce the design and implementation of system for health examination and health monitoring according to the needs and workflow of the Health Examination Center. Methods The system has an effective configuration based on HL7 and smart client technology. Results The system can truly meet the actual needs and realize the digitizing management of the health examination center. Conclusion The system plays an important role in optimizing the wotkflow and can improve service quality and efficiency of health examination to promote digital construction in hospital.