2.Mission: halt diabetes momentum and stop growing epidemic.
Chinese Medical Journal 2008;121(21):2115-2118
4.Transvaginal ultrasound guided methotrexate injection for unruptured early tubal pregnancy
Wen YANG ; Yu FANG ; Ying WANG
Chinese Journal of Minimally Invasive Surgery 2005;0(07):-
2 000 mIU/ml,combined use of mifepristone(100 mg,twice daily,for 3 days) was employed. Results The treatment succeeded in 28 patients,with a success rate of 87.5%.Acute lower abdominal pain occurred in 4 patients and laparoscopic salpingectomies were carried out.The serum ?-hCG levels decreased by 23%~35% at 3 days after injection,by 38%~62% at 1 week,and to normal levels(≤6.1 mIU/ml) at 2~4 weeks.The mass completed subsided at 30~55 days after injection. Conclusions Transvaginal ultrasound guided methotrexate injection for the management of unruptured early tubal pregnancy is simple,safe, and effective.Further investigations should be carried out in respect of the recovery of tubal anatomy and physiological functions.
5.Effects of internal and external biliary drainage on inducible nitric oxide synthase mRNA expression of Kupffer cell and serum tumor necrosis factor-alpha in rats with obstructive jaundice
Ying MENG ; Yunsheng YANG ; Wen LI
Chinese Journal of Digestion 2008;28(10):669-672
Objective To study the effects of internal and external biliary drainage on inducible nitric oxide synthase (iNOS) mRNA expression of Kupffer cells and serum tumor necrosis factor-alpha (TNF-α) in rats with obstructive jaundice. Methods Forty-eight male Sprague-Dawley rats were randomly assigned to obstructive jaundice (OJ), sham operation (SH), internal biliary drainage (ID) and external biliary drainage (ED) groups with 12 each. Kupffer cells were isolated by in situ hepatic perfusion and digestion with collagenase type Ⅳ, and purified by cell culture attachment. The expression of iNOS mRNA of Kupffer cells was detected by reverse transcription polymerase chain reaction (RT-PCR) and the serum TNF-α concentration was measured using ELISA method. Results The serum TNF-α level was increased in OJ group[ (110.84±26.3) pg/ml ]compared with SH group [-(88.4±17.9) pg/ml, (P=0.045)]. The TNF-α level in ID group[ (89.8±28. 3) pg/ml ]was significantly lower than that in ED group[ (118.64±22.7) pg/ml, (P=0. 011) ]and OJ group (P=0. 059). Expression of iNOS mRNA of Kupffer cells was significantly higher in OJ group (0. 824± 0. 24) compared with SH group (0. 384±0.35,P=0. 005). After relieving the OJ, the iNOS mRNA expression in ED group (0. 974± 0.48) was not suppressed (P=0. 321). On the contrary, the iNOS mRNA expression in ID group was suppressed and significantly lower than that in ED group (0. 59±0. 35) (P=0. 016). Conclusions Internal biliary drainage is superior to external drainage in terms of reversing the elevated serum TNF-α and in suppressing the iNOS mRNA expression of Kupffer cells in rats with obstructive jaundice.
6.Surgical treatment of cervical ossification of posterior longitudinal ligament with high-signal intensity zone and analysis of the curative effect
Yang LI ; Wen YUAN ; Ying ZHANG
Orthopedic Journal of China 2006;0(01):-
[Objective]To observe and analyze the clinical results of ossification of posterior longitudinal ligament(OPLL) combined with high-signal intensity zone(HIZ) change. [Methods]Forty patients with OPLL combined with HIZ were treated from March 2004 to March 2008.Anterior approach surgery was given to 19 cases as group A and posterior approach surgery was given to 21 cases as group P.All cases were followed up.The pre-and post-operative JOA17 scores and improvement ratio were evaluated.[Results]The mean follow-up time were(19.5?3.4) months in group A and(20.3?4.5) months in group P.The mean improvement ratio were(56.2?8.4)% in group A and(52.2?12.2)% in group P.There were no statistical differences between those of groups A and P.Mean JOA scores at last follow-up were(12.8?0.9) in group A and(11.5?1.6) in group P,with significantly difference.[Conclusion]Treatment of OPLL combined with HIZ is a high risk procedure with limited recovery in neoro-function.Good surgical result could be got with correct judgement of type and location of OPLL,occupation ratio of spinal canal and selection of approach.
8.Pharmacokinetics of SN-38 in rats and tissue distribution of 7-ethyl-10-hydroxycamptothecin in mice after intravenous injection of irinotecan hydrochloride nanoparticles.
Fu-Ying YANG ; Wen-Ping ZHANG ; Xin-Yu WANG ; Wen-Cheng YANG ; Hong-Wan DANG
Acta Pharmaceutica Sinica 2014;49(7):1029-1033
The paper reported an investigation of the pharmacokinetics of SN-38 (7-ethyl-10-hydroxy-camptothecin) in rats and the tissue distribution in mice after injection of irinotecan hydrochloride nanoparticles (CPT-11) via tail veins. An LC-MS/MS method was established to determine the concentrations of SN-38 in whole blood of rats and in different tissues of mice. The pharmacokinetics and tissue distribution of SN-38 were compared after the intravenous injection of CPT-11 NPs and CPT-11 solution. Compared with irinotecan solution, the elimination half-life of SN-38 was prolonged from 2.17 h to 2.67 h after the intravenous injection of CPT-11 NPs, but its AUC had little change. After the injection of CPT-11 NPs in mice, over time, the concentrations of CPT-11-metabolized SN-38 in CPT-11 NPs were significantly higher in the whole blood, colon and lungs than those in CPT-11 solution, followed by in the spleen and liver, but those in the heart and brain had no change. However, the amount of SN-38 in the kidneys was reduced with time. CPT-11 NPs could prolong SN-38's (one of its metabolites) blood circulation time in rats and significantly increased the concentration of CPT-11-metabolized SN-38 in the whole blood, colon and lungs of mice. CPT-11 NPs made SN-38 efficiently target-bind to the colon and lungs of mice.
Animals
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Antineoplastic Agents, Phytogenic
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pharmacokinetics
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Camptothecin
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analogs & derivatives
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pharmacokinetics
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Chromatography, Liquid
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Colon
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metabolism
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Half-Life
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Injections, Intravenous
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Lung
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metabolism
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Mice
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Nanoparticles
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administration & dosage
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Rats
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Tandem Mass Spectrometry
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Tissue Distribution
9.A case of Guillain-Barre syndrome complicated with posterior reversible encephalopathy syndrome.
Ying YANG ; Jian-hua FENG ; Yu-wen DAI
Chinese Journal of Pediatrics 2013;51(6):477-478
Biomarkers
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blood
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Brain
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diagnostic imaging
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pathology
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Brain Edema
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etiology
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pathology
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Child
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Diagnosis, Differential
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Electroencephalography
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Guillain-Barre Syndrome
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complications
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diagnosis
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therapy
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Humans
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Magnetic Resonance Imaging
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Male
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Posterior Leukoencephalopathy Syndrome
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complications
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diagnosis
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therapy
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Radiography
10.Determination of Four Kinds of Ingredients in Transparent Absorbent Fluid of Different Size of Huoxue Powder by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
Binghuo YANG ; Fuqiong ZHOU ; Weina ZHU ; Wen LI ; Ying ZHAO
Herald of Medicine 2017;36(9):1033-1037
Objective To optimize the particle size of Huoxue powder by contents comparison of emodin,phellodendrine,berberine and jatrorrhizine before and after permeabilized skin absorption of Huoxue powder in different particle size of 0.150,0.075,0.048,0.038 mm.Methods The contents of emodin,phellodendrine,berberine and jatrorrhizine in transparent absorbent fluid of Huoxue power in different particle size within 24 hours were measured by ultra performance liquid chromatography tandem mass spectrometry,and optimize its particle size by contents comparison of the effective components.Results The contents of the active components in Huoxue power with the particle size of 0.075 mm were high before and after percutaneous absorption.Conclusion Particle size of 0.075 mm is best for Huoxue powder.