1.Mechanism research of miR - 181 regulating human lens epithelial cell apoptosis
Yu, QIN ; Jiang-Yue, ZHAO ; Wen-Ting, LUO ; Jing, LI ; Jia, LIU ; Jin-Song, ZHANG
International Eye Science 2015;(5):759-763
?AlM: To investigate the expression of miR-181 in the lens tissue of cataract and the regulating mechanism of miR-181 on apoptosis of human lens epithelial cell.
?METHODS:Real time q-PCR was used to measure the expression of miR-181 in the anterior lens capsules of age - related cataract and human lens epithelial cell apoptosis model. miR- 181 mimic and inhibitor were transfected using Lipofectamine 2 000 to regulate the expression of miR-181, and then Real time q-PCR was used to verify transfection efficiency. Flow cytometry was used to detect the change of cell apoptosis rate.
? RESULTS: Compared with control group, the expression of miR-181 was significantly higher in both the anterior lens capsules of age-related cataract and human lens epithelial cell apoptosis model; the relative expression of miR-181 in lens epithelial cells transfected with miR-181 mimic was increased, whereas decreased in cells transfected with miR-181 inhibitor;the apoptosis rate of cells transfected with miR - 181 mimic was increased, while reduced in miR-181 inhibitor group. Each result was statistically significant (P<0. 01).
?CONCLUSlON:High expression of miR-181 is detected in anterior lens capsule of age-related cataract. miR-181 might play a certain role in the pathogenesis of cataract via promoting human lens epithelial cell apoptosis. miR-181 probably becomes a new approach for the nonoperative treatment of cataract, but the concrete mechanism still needs to be further studied.
2.Effect of Different Electron Donors on Reductive Dechlorination of 2,4-Dichlorophenol
Ting CHENG ; You-Zhi DAI ; Chun-Xiang LUO ; Shuang-Shuang LI ; Wen-Qi TANG ;
Microbiology 2008;0(08):-
A test was conducted to examine the effect of several electron donors such as glucose, sodium acetate, Fe0, Fe0+glucose and Fe0+sodium acetate on reductive dechlorination of 2,4-dichlorophenol (2,4-DCP) through inoculating the unacclimated anaerobic mixed bacteria. The optimum condition and sus-tainability of Fe0 as electron donor was also been discussed. The results showed that, Fe0+glucose enhanced the dechlorination of contaminant effectively compared to glucose. Sodium acetate, Fe0 and Fe0+sodium acetate were all effective electron donors and Fe0 was the optimum, the optimum initial pH was 8.0 and quantity of added Fe0 was 2.0 g/L. 4-CP was the mainly intermediate product for 2,4-DCP dechlorination. Fe0 could support the electron for reductive dechlorination of 2,4-DCP continuously. In contrast, when so-dium acetate as electron donor, the effect of dechlorination was inferior to Fe0 with the consumption of sodium acetate.
3.Clinical observation on acupoint sticking therapy plus electroacupuncture for treating peripheral facial paralysis
Li-Mei FENG ; Ting-Ting ZENG ; Wen-Guang HOU ; Yu-Dan ZHU ; Kang WANG ; Feng-Hui JIANG ; Wen-Min YUAN ; Hui CHEN ; Jin-Chao LUO
Journal of Acupuncture and Tuina Science 2020;18(6):445-451
Objective: To observe the clinical effect of acupoint sticking therapy with Mian Tan Gao (facial paralysis paste) plus electroacupuncture (EA) for treating peripheral facial paralysis and its influence on patients' facial nerve functions, facial disability index and clinical symptoms and signs. Methods: A total of 96 peripheral facial paralysis patients were allocated into an observation group, a medicine group and an EA group by simple randomization, with 32 cases in each group. Patients in the medicine group were treated with oral mecobalamine and prednisone acetate; patients in the EA group were treated with EA on the basis of the medicine treatment; while patients in the observation group were treated with acupoint sticking therapy with Mian Tan Gao (facial paralysis paste) plus EA. After 4-week treatment, the clinical efficacy, the adverse events, and the scores of House-Brackmann (H-B) facial nerve function grading scale, visual analog scale (VAS), clinical symptoms and signs, and facial disability index (FDI) were compared. Results: After 4-week treatment, the total effective rate was 96.9% in the observation group, higher than 68.7% in the medicine group and 75.0% in the EA group (both P<0.05). After 4-week treatment, the scores of H-B grading scale, VAS and clinical symptoms and signs in the three groups dropped significantly compared with those before treatment, and the scores in the observation group were lower than those in the medicine group and EA group (all P<0.05). After 4-week treatment, the facial disability index-physical function (FDIP) in the FDI in the three groups increased significantly, with a higher value in the observation group compared with that in the medicine group and EA group (both P<0.05). The facial disability index-social function (FDIS) in the FDI dropped significantly, with a lower score in the observation group compared with that in the medicine group and EA group (both P<0.05). However, the comparisons of the items above between the medicine group and the EA group showed no statistical significance (all P>0.05). The between-group comparison of the adverse event across the three groups showed no statistical significance (P>0.05). Conclusion: Acupoint sticking therapy with Mian Tan Gao (facial paralysis paste) plus EA can decrease H-B grade, reduce pain severity and improve clinical symptoms and signs as well as the facial disability condition in peripheral facial paralysis patients. This method produced more significant efficacy compared with oral medicine and medicine plus EA.
4.Study on thinking of clinical scientific research of acupuncture and moxibustion.
Yi GUO ; Ting LUO ; Qing-Wen LI
Chinese Acupuncture & Moxibustion 2005;25(1):3-6
Under guiding of correct thinking of scientific research, development of clinical scientific research of acupuncture and moxibustion is of important significance for promoting academic progresses of acupuncture and moxibustion, increasing clinical therapeutic effect of acupuncture and moxibustion. This paper probes thinking of clinical scientific research of acupuncture and moxibustion and puts forward the guiding principles, i.e. testing and verifying therapeutic effect, searching for laws, expounding mechanisms and guiding clinical practice.
Acupuncture
;
Acupuncture Therapy
;
Biomedical Research
;
Humans
;
Moxibustion
5.An improved morphological edge detection algorithm of medical image based on multi-structure element.
Xiaogang LUO ; Ting LIU ; Chenglin PENG ; Li WEN
Journal of Biomedical Engineering 2009;26(1):177-183
An improved edge detection algorithm is proposed in this paper for the medical images with strong noises and fuzzy edges. The algorithm modified the combination of morphological operations, so that the unclear edges of the images are avoided. In this paper is also introduced the algorithm of multi-structure elements which can reserve integrated edges from different directions of the images. Furthermore, the contrast enhancement and morphological filter processing are implemented. This method can detect the edges efficiently, keep the detected edges smooth and obtain coherent image edges. Experiments demonstrate that this edge detector has a better performance of noise reduction and keeps the edges more accurate than do the traditional edge detectors; thus its practicality is enhanced.
Algorithms
;
Humans
;
Image Enhancement
;
Image Processing, Computer-Assisted
;
methods
;
Tomography, X-Ray Computed
6.Effects of transcatheter arterial chemoembolization with pingyangmycin-lipiodol emulsion on VX2 liver tumors in rabbits.
Xi LIU ; Xiao-ping LUO ; Wen-ting CAO ; Hao DENG
Chinese Journal of Hepatology 2012;20(8):611-616
To evaluate the changes induced in tumor tissue, the feeding artery, and neovascularization upon pingyangmycin-lipiodol emulsion treatment via transcatheter arterial chemoembolization (TACE) using the rabbit VX2 liver cancer model. The VX2 liver tumor model was established in 28 rabbits, and baseline tumor volume (V1, in mm3) was measured by spiral scan computed tomography (CT). Then, the rabbits were randomly divided into four groups (n = 7 each) and administered intraarterial therapies of: ultrafluid lipoidol embolization (group A); pingyangmycin (group B); pingyangmycin-lipiodol emulsion (group C); or saline (group D). All rabbits were sacrificed seven days later, and the response to therapy was determined by measuring the tumor volume (V2, in mm3), calculating the tumor growth rate, detecting expression of the vascular endothelial growth factor (VEGF) tumor biomarker, and performing histological analysis of the microvessel density (MVD) in the liver. Prior to therapy, the average V1 of the groups was statistically similar (A: 389.8+/-167.3, B: 404.1+/-184.9, C: 355.1+/-158.3, D: 378.1+/-189.0; (F = 0.257, P more than 0.05). In contrast, after therapy the average V2 of the groups was significantly different (A: 922.6+/-32.9, B: 665.9+/-99.9, C: 349.5+/-177.8, D: 1403.5+/-411.2; F = 26.23, P less than 0.05), as was the tumor growth ratio (A: 1.4, B: 0.6, C: -0.02, D: 2.7) and the mean positive ratio of VEGF (A: 57.1%, B: 42.9%, C: 28.6%, D: 100%; F = 8.407, P less than 0.05). MVD was highest in group D and lowest in group C (all, P less than 0.05). Bivariate correlation analysis revealed a positive correlation between VEGF expression and MVD (r = 0.743, P less than 0.01). Pingyangmycin exerts anti-tumor effects in the rabbit VX2 liver cancer model, but is more effective when administered as the combination therapy of pingyangmycin-lipiodol emulsion with TACE.
Animals
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Antibiotics, Antineoplastic
;
administration & dosage
;
therapeutic use
;
Bleomycin
;
administration & dosage
;
analogs & derivatives
;
therapeutic use
;
Chemoembolization, Therapeutic
;
methods
;
Emulsions
;
Ethiodized Oil
;
administration & dosage
;
therapeutic use
;
Female
;
Iodized Oil
;
administration & dosage
;
therapeutic use
;
Liver Neoplasms, Experimental
;
blood supply
;
drug therapy
;
pathology
;
Male
;
Microvessels
;
Neoplasm Transplantation
;
Neovascularization, Pathologic
;
Rabbits
;
Random Allocation
;
Tumor Burden
;
drug effects
;
Vascular Endothelial Growth Factor A
;
metabolism
7.Relationship of urine cobalt and individual exposure levels of cobalt and its compounds in cobalt exposed workers
LUO Xiao ting CHEN Wen DONG Ming WU Chuan LIN Zuo kan TAN Jian ming WU Bang hua
China Occupational Medicine 2022;49(06):615-620
Objective -
To analyze the relationship between cobalt level of post shift urine and individual exposure level of
,
cobalt and its compounds in cobalt exposed workers and to explore the feasibility of using urine cobalt as a biomarker.
Methods -
A total of 148 occupational cobalt exposed workers from a new material company were selected as the exposed
, - -
group and 44 non occupational cobalt exposed workers from the company were selected as the control group using the typical
sampling method. The exposure concentration of time weighted average of cobalt and its compounds in the workplace air of the
-
two groups was determined by inductively coupled plasma mass spectrometry as the individual exposure level. The cobalt levels
- -
of pre shift and post shift urinary samples of the two groups were detected by this method. The linear relationship between the
-
cobalt level of post shift urine and the individual exposure level of cobalt and its compounds in the air of the workplace was
Results - 3
analyzed. The individual exposure level of cobalt and its compounds in the exposed group was 1.10 131.71 μg/m with
(M) 3
the median of 12.23 μg/m. No cobalt and its compounds were detected in the workplace air in the control group. The cobalt
- -
levels of pre shift and post shift urines in exposed group were higher than those in the control group at the same time point
(M: vs , vs , P ) - -
1.54 0.56 μg/L 8.77 0.83 μg/L all <0.01 . The cobalt level of post shift urine was higher than that in pre shift
(M: vs ,P ),
urine in the exposed group 8.77 1.54 μg/L <0.01 and it was positively correlated with the individual exposure level
( ,P ) ,
of cobalt and its compounds Spearman correlation coefficient=0.86 <0.01 . After common logarithm conversion the linear regression equation of the cobalt level of post shift urine and the common logarithm of individual exposure level of cobalt and
(x) :ŷ x( ;F ,
its compounds in the exposed group was as follows = −0.178 + 0.988 coefficient of determination=0.72 =374.75
P ;t , P ) Conclusion -
<0.01 = - 19.36 <0.01 . There was a linear correlation between cobalt level of post shift urine and
occupational cobalt exposure level of cobalt exposed workers. Urinary cobalt can be used as a biomarker of occupational cobalt
8.Effects of syndrome-differentiation acupuncture on life quality in patients with functional dyspepsia.
Chao-Yang MA ; Qi HUANG ; Wen-Jun WAN ; Li ZHOU ; Lei TANG ; Yan Fu WANG ; Qin-Qin LUO ; Ting ZHANG ; Ying WANG
Chinese Acupuncture & Moxibustion 2014;34(2):125-129
OBJECTIVETo observe effects of syndrome-differentiation acupuncture on life quality in patients with functional dyspepsia (FD) in order to evaluate its clinical efficacy.
METHODSOne hundred and five cases of FD were randomly divided into a syndrome-differentiation acupuncture group, a regular acupuncture group and a non-acupoint group, 35 cases in each one. Zhongwan (CV 12), Tianshu (ST 25), Zusanli (ST 36) were selected as main acupoints in the syndrome-differentiation acupuncture group. After syndrome differentiation, Danzhong (CV 17) and Zhangmen (LR 13) were added for those with stagnation of liver qi; Pishu (BL 20) and Weishu (BL 21) were added for those with deficiency of spleen-stomach qi; Qimen (LR 14) and Taichong (LR 3) were added for liver-qi invading stomach and Yinlingquan (SP 9) and Neiting (ST 44) were added for dampness-heat blocking stomach. The selection of acupoints in the regular acupuncture group was the same as main acupoints in the syndrome-differentiation acupuncture group. The points 10 mm lateral to the main acupoints were selected in the non-acupoint group. The treatment was given once a day, six days as a treatment course and totally two courses were required. The symptom total score, health-related quality of life survey (SF-36) and Nepean dyspepsia index (NDI) were evaluated before and after the treatment as well as one month after the treatment (follow-up visit), respectively. The efficacy was also assessed.
RESULTSAfter the treatment, the total effective rate was 87.5% (28/32) in the syndrome-differentiation acupuncture group, which was superior to 74.2% (23/31) in the regular acupuncture group and 20.7% (6/29) in the non-acupoint group (P < 0.05, P < 0.01). Compared before the treatment, the SF-36, NDI and symptom total score after the treatment and in the follow-up visit were all obviously improved in the syndrome-differentiation acupuncture group and regular acupuncture group (all P < 0.05), which was the most obvious in the syndrome-differentiation acupuncture group [after the treatment, SF-36: 84.54 +/- 5.93 vs 81.44 +/- 6.22, 63.46 +/- 6.59; NDSI: 18.94 +/- 9.30 vs 21.23 +/- 8.39, 43.93 +/- 11.26; NDLQI: 71.42 +/- 7.23 vs 63.11 +/- 7.06, 54.87 +/- 6.00; symptom total score: 22.06 +/- 15.80 vs 32.52 +/- 16.88, 47.97 +/- 10.92]; the improvement in the regular acupuncture group was more obvious than that in the non-acupoint group (P < 0.01, P < 0.05). Compared before the treatment, only NDSI score was improved in the non-acupoint group after the treatment (P < 0.05).
CONCLUSIONThe syndrome-differentiation acupuncture could obviously improve patient's life quality in the treatment of FD, which is an effective therapy for FD.
Acupuncture Points ; Acupuncture Therapy ; Adult ; Dyspepsia ; physiopathology ; psychology ; therapy ; Female ; Humans ; Male ; Middle Aged ; Quality of Life ; Young Adult
9.Preparation optimization for Bishutongqiao Mixture
huan Jie LI ; lin Luo WANG ; Ming GUO ; jie Wen XU ; ting Xue CHEN ; yong Zhi LI
Chinese Traditional Patent Medicine 2017;39(9):1825-1829
AIM To optimize the preparation of Bishutongqiao Mixture.METHODS With solid total mass,contents of astragaloside A and calycosin-7-glucoside as evaluation indices,water addition,extraction time and extraction times as influencing factors,the aqueous extraction process was optimized by orthogonal test.With the contents and retention rates of astragaloside A and calycosin-7-glucoside as evaluation indices,the effects of normal pressure and decompression on the concentration process were investigated.And the effects of solubilizer (polysorbate 80),taste-masking agent (sucrose) and bacteriostatic agent (sodium benzoate) amounts on the forming process were studied as well.RESULTS The optimal aqueous extraction conditions were determined to be ten times for water addition,1 h for extraction time,and extracting twice,the comprehensive score was 91.67.The contents and retention rates of astragaloside A and calycosin-7-glucoside at normal pressure concentration were higher than those at decompression concentration.The optimal forming process was obtained at polysorbate 80,sucrose and sodium benzoate amounts of 1.43%,15% and 0.3%,respectively.CONCLUSION This stable and feasible method can be used for the preparation of Bishutongqiao Mixture.
10.Role of phospholipase C-gamma1 signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells.
Wen-Li YUAN ; Di LU ; Jun SUN ; Guang-Xue CHEN ; Hui CHEN ; Ting-Hua WANG ; Shen-Qiu LUO
Journal of Southern Medical University 2008;28(11):1939-1941
OBJECTIVETo explore the role of phospholipase C-gamma1 (PLC-gamma1) signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells.
METHODSPC12 cells were exposed to 50 micromol/L H(2)O(2) after pretreatment with 10 micromol/L U73122, a specific PLC-gamma1 inhibitor. Hoechst/PI double staining was performed to observe the morphological changes of the cells under light microscope. MTT assay was used to evaluate the cell viability, and the percentage of apoptotic cells was analyzed by flow cytometry. DNA fragmentation assay was carried out to characterize the cell apoptosis.
RESULTSAfter inhibition of the PLC-gamma1 signaling pathway with 10 micromol/L U73122, PC12 cells showed obvious apoptotic morphology, the viable cells decreased significantly, and the percentage of apoptotic cells rose to 35.7%. PC12 cells treated with U73122 presented with a distinct DNA ladder on electrophoresis resulting from DNA cleavage in the apoptotic cells.
CONCLUSIONPLC-gamma1 signaling pathway plays an important protective role in H(2)O(2)-induced PC12 cell apoptosis.
Animals ; Apoptosis ; drug effects ; Estrenes ; pharmacology ; Hydrogen Peroxide ; pharmacology ; PC12 Cells ; Phospholipase C gamma ; antagonists & inhibitors ; metabolism ; Pyrrolidinones ; pharmacology ; Rats ; Signal Transduction