Objective: To establish the Perfect Systematization and Informatization for the Management of Medical Equipment Archive, so as to ensure the integrity and traceability of each data. Methods: Analysis on Present Situation and the Problems of Medical Equipment Management, Establish Medical Equipment archive Management System from purchasing of medical equipment, Measurement Controls, the Maintenance to Scrap processing the whole-process of medical devices. Results: In this paper, we discussed all kinds of information and archives network information management in the whole-process of medical devices. To clarify the plan, argument, purchase, installation, acceptance, use, quality control, maintenance, transfers and scrap processing, management at different levels. To establish a complete electronic file system is the main direction of medical equipment information management in the future. Conclusion: Realization of the functions on medical equipment archives network information and dynamic management, that can offer necessary for safe treatment,to can also reduce the operation cost of the equipment.

Objective:This paper makes analysis of problems of emergency equipment existing in clinical application and their causes and puts forward corresponding measure. Methods:Analysis of emergency medical equipment characteristic and the difference between conventional equipment. Find the first aid equipment management problems and reasons existing in the use, and risk assessment. Results:Hospital first aid equipment has the characteristics of more free time, emergency and risks, widely distributed in each department. By the personnel management, periodic inspection, cleaning and disinfection. Risk assessment shall be conducted by experts, the risk value high priority arrangement first aid equipment maintenance, to expect to minimize risk. Conclusion:First aid equipment management is a systematic and important work, to need department, maintenance personnel, equipment manufacturers and so on various aspects to cooperate with each other, to ensure the normal use of equipment.

Objective: To improve the whole-process management of medical devices in purchase, use and maintenance. Methods: In this paper, we clarify the purchase, use and maintenance requirements of large-scaled medical devices including justification, invitation organization, comprehensive estimation, contract signing protocol, advantage analysis, rational control of availability, efficacy measurement and detection, management at different levels, high-efficacy check-up and scrap procedure to adapt the new challenges during the introduction of more and more advanced, complex and high-tech medical devices. Results:The results being analyzed could be used as important evidence for future purchasing of medical equipment, which was important for scientific and reasonable purchasing and utilizing of medical equipment. Conclusion: Strengthen the management to adapt the new challenges during the introduction of more and more advanced, complex and high-tech medical devices.

OBJECTIVETo observe the effect of Wenyang Huoxue Lishui Recipe (WHLR) containing serum on the expression of cathepsin L (CatL) in puromycin aminonucleoside-induced injury of mouse glomerular podocytes.

METHODSMouse podocyte cells (MPCs) in vitro cultured were divided into the normal control group, the model group, the dexamethasone (DEX) group, 10% WHLR containing serum group, 20% WHLR containing serum group, the vehicle serum control group. MPCs in the normal control group were cultured at 37 degrees C culture solution for 24 h. 45 mg/L puromycin was acted on MPCs in the model group for 24 h. On the basis of puromycin intervention, 1 limol/L DEX was co-incubated in MPCs of the DEX group for 24 h; 10% or 20% WHLR containing serum was co-incubated in MPCs of the 10% WHLR containing serum group and 20% WHLR containing serum group for 24 h. The vehicle serum control group was also set up by incubating with WHLR containing serum alone for 24 h. The expression of CatL and its substrate Synaptopodin in podocytes were detected by cell immunofluorescence staining. FITC-conjugated phalloidin was used to stain F-actin. A cortical F-actin score index (CFS index) was designed to quantify the degree of cytoskeletal reorganization in cultured podocytes.

RESULTSCompared with the normal control group, the expression of synaptopodin significantly decreased and the expression of CatL significantly-increased in the model group. F-actin arranged in disorder, gradually forming pericellular F-actin ring. CFS index was obviously elevated (P < 0.01). Compared with the model group, the epression of synaptopodin increased, the expression of CatL decreased, and CFS index also decreased in the DEX group, 10% WHLR containing serum group, and 20% WHLR containing serum group (P < 0.05, P < 0.01). Compared with the DEX group, the expression of synaptopodin decreased in 10% WHLR containing serum group, CFS index also decreased in 20% WHLR containing serum group (P < 0.05).

CONCLUSIONSWHLR could up-regulate the expression of synaptopodin, down-regulate the expression of CatL, and alleviate cytoskeletal reorganization of F-actin. It was helpful to stabilize the cytoskeleton of F-actin and improve the merging of podocytes.

Actins ; metabolism ; Animals ; Cathepsin L ; metabolism ; Cells, Cultured ; Down-Regulation ; Drugs, Chinese Herbal ; pharmacology ; Kidney Glomerulus ; cytology ; Mice ; Microfilament Proteins ; metabolism ; Podocytes ; drug effects ; pathology ; Puromycin Aminonucleoside ; adverse effects ; Up-Regulation

OBJECTIVE: To investigate the effect of bromocriptine on the expression of Pit-1 in prolactinoma rats. METHODS: Firstly, to prepare prolactinoma model in rats. Adult Wistar rats were divided into two groups at random. The rats in control group were subscutaneously implanted with a blank implant. Rats in 17β-estradiol group were implanted with 17β-estradiol-containing implants. Secondly, rates in 17β-estradiol group were divided into two groups at random: model group and bromocriptine group. Water was administrated to rats in model group. Bromocriptine (0.225 mg · kg-1 · d-1) were orally administrated to rats in bromocriptine group, the rats in control group were orally administrated with water. After four weeks of treatment, all the animals were executed. Each pituitary gland was weighed. Serum prolactin (PRL) levels were measured by RIA method. Pit-1 mRNA levels in pituitaiy tissue were measured by RT-PCR method. RESULTS: The weights of pituitary gland and PRL levels in 17β-estradiol group were individually higher than those in control group (P<0.001). The expression levels of Pit-1 mRNA in bromocriptine group was obviously lower than that in model group (P≤0.001). CONCLUSION: Bromocriptine has potential preventive effect to estrogen-induced rat prolactinoma. The decrease of Pit-1 mRNA level may be involved in the mechanism of anti-prolactinoma effect of bromocriptine.

Humans ; Occupational Health Physicians ; organization & administration ; Personnel Selection

Cancer stem cells is a small fraction of cells exist in cancers and own the same characteristics of stem cells. It has recently been shown that cancer stem cells in some tumours contain lower reactive oxygen species(ROS) levels and enhanced ROS defence radioresistance. The DNA damage induced by p53 tumor suppressor protein is associated with self cancer stem cells. We have reviewed the biological characteristics including self- renewal, drug resistance, heterogeneity and so on. As to the genesis of the cancer stem cells,some researchers believe that they arise from normal adult stem cells, progenitor cells or mutation of somatic cells, while others consider that cancer stem cells are derived from cell fusion and so on.

Cells, Cultured ; Flutamide ; pharmacology ; Human Umbilical Vein Endothelial Cells ; cytology ; drug effects ; metabolism ; Humans ; Proto-Oncogene Proteins c-myb ; metabolism ; Sp1 Transcription Factor ; metabolism ; Testosterone ; antagonists & inhibitors ; physiology ; Transcription Factors ; metabolism ; Tumor Suppressor Protein p53 ; metabolism

Objective To investigate the causes and preventive measures of upper gastrointestinal bleeding in hyperbaric oxygen therapy for patients with severe closed head injury.MethodsThe clinical data of 24 cases of patients with upper gastrointestinal hemorrhage treated by hyperbaric oxygen therapy in the first people's Hospital of Fuyang District Hangzhou from May 2011 to May 2016 were analyzed, the occurrence time and clinical effect of upper gastrointestinal bleeding were analyzed.ResultsAmong the 24 cases of patients, 14 cases were treated for the first time that overt bleeding, including bleeding from the stomach liquid outflow of blood from 2 cases, gastric outflow 2 cases, hemorrhage after cure again received hyperbaric oxygen therapy 3 times again dominant bleeding 2 cases;6 cases hyperbaric oxygen treatment for third, 7, 10 times brown liquid or bloody fluid from the stomach outflow;4 cases hyperbaric oxygen treatment in patients with second and 3 courses of coffee liquid from the outflow tube, which accounted for 58.3%, 25.0%, 16.7% of the total, retrospectively;8 cases were with good recovery, 10 cases moderate disability, 4 cases severe disability, 2 cases vegetative state, 0 case died, the good recovery rate was 33.3%.ConclusionNot correctly grasp the time of hyperbaric oxygen therapy in the treatment of patients with severe closed craniocerebral injury will cause upper gastrointestinal hemorrhage, correct application of H2 receptor binding agent, developing targeted therapy programme can effectively prevent and treat upper gastrointestinal bleeding, so is worthy of the clinical's full attention.

OBJECTIVE:To prepare chlorphenamine cream,establish its quality control and observe its clinical therapeutic effect.METHODS:Chlorphenamine cream was prepared by emulsification,the content was determined by UV spectrophotom?etry and the stability of which was observed by the initial average rate method.RESULTS:The linear range of chlorphenamine was10～30?g/ml(r=0.9999);the average recovery rate was101.25%,RSD=1.28%;the total effective rate in the clinical application was97.66%.CONCLUSION:The cream is stable in quality and its therapeutic effect is reliable and safe.