Objective To observe the influence of Dunhuang Nourishing Cream on the contents of SOD, GSH-PX and MDA in skin of aged rats. Methods Female SD rats of SPF level were divided into 6 groups:high, moderate and low dose groups of Dunhuang Nourishing Cream, model contrasting group, positive contrasting group and blank contrasting group. After removed the hair on the back, the drug groups were applied with Dunhuang Nourishing Cream, the model group was applied with the matrix of Dunhuang Nourishing Cream and the positive group was applied with vitamin E. After 30 days, the contents of SOD, GSH-PX and MDA in the skin were tested with the method of biochemistry analysis. Results Compared with the model group, the contents of SOD, GSH-PX and MDA in the high, moderate and low dose groups had significant difference (P

Objective To investigate the clinical efficacy and safety of laparoscopic appendectomy for acute appendicitis.Methods Nighty-two cases of acute appendicitis were included in this study,randomly divided into observation group (n =46) and control group (n =46) according to the random number table method.The observation group was treated with laparoscopic appendectomy,and control group with the traditional open appendectomy treatment.We compared the two groups of cases in postoperative conditions and the level of WBC changes and 1d and 3d after operation.Results The operation time of the observation group was shorter than that of the control group (t=4.049,P＜0.05);there was no significant difference in lymph nodes (t=0.570,P＞0.05);the amount of bleeding was less than that of the control group (t=20.748,P＜0.05).The get-out-of-bed activity time in the observation group was faster than the control group (t=9.221,P＜0.05);eating time was faster than the control group (t=8.794,P＜0.05);the anus recovery exhaust time was faster than the control group (t=17.423,P＜0.05);hospital stay was shorter than the control group (t=13.479,P＜0.05);the complication rate was lower than that of the control group (t=13.883,P＜0.05).The levels of 1d and 3d WBC were lower in the observation group than that in the preoperative group (t=7.277,26.911,P＜0.05);the levels of 1d and 3d WBC in the observation group were lower than those in the control group at the same time (t=3.6189,17.827,P＜0.05);the 3d WBC level of the observation group was lower than that of 1d (t=20.732,P＜0.05).Conclusion Laparoscopic appendectomy is a safe and effective treatment for patients with acute appendicitis,and it is worthy of further clinical research.

The WAN Mi-zhai's health preserving thought includes guayu,shendong,fashi and queji .The key of guayu is to control appetition to conserving essence.The aim of shendong is to keep the good psychology with peaceable mind.The view-point of fashi is to keep up with nature for harmonizing yin and yang.The denotation of queji is to prevent disease and regulate essence, qi and spirit.

The organophosphorus insecticides including a large group of insecticides were widely used in the world in the agriculture and household. Over 80% of the pesticides used in China are organophosphorus pesticides. Workers and the general population may be exposed to insecticides mixtures through water,air and the residue of food. After entering into human body,the insecticide mixtures can cause a combined toxicity. In this paper,the effects and toxicity mechanisms of combined exposures on health were expounded.

Objective To observe the dynamic levels of IL-2,IL-5 and IL-6 produced by Balb/ c mice infected with DEN_2 clinical strains and to study their relation.Methods The Balb/c mouse in- feetion model was established by multiple-site subcutaneous injection with various doses of DEN_2 clini- cal strain.Mouse plasma samples collected from different experiment groups at various time after in fection were tested for IL-2,IL-5 and IL-6 levels with sandwich ELISA.Results After primary in- feeted with DEN_2 B strain,the levels of IL-2,IL-5 and IL-6 of all experimental groups were not sig- nificantly higher than the normal control group while the levels of experimental groups increased sig- nificantly after re-infection.The level of IL-2 reached to peak[average value of(101 522.44?10 465.375)pg/ml]at the 4th day after re-infection(the 20th day after the primary infection),and then the level gradually reduced.The levels of IL-5 in the Balb/c mice of the group 1 and 2 reached to peak at the 1st day after re infection(the 16th day after the primary infection),and there was signifi- cant difference between these two groups and the control group(P＜0.05).The levels of IL-6 in all experimental groups reached to peak at the 1st and the 2nd day after re-infection.The peak value of the third group is the highest comparing with the normal control group(P＜0.05).Conclusion Th2 response was predominant in the second infection phase.

Objective To explore the curative effect of combined use of minimally invasive percutaneous nephrolithotomy (MPCNL) and extracorporeal shockwave lithotripsy (ESWL) for the treatment of solitary renal staghorn calculi in patients with renal insufficiency. Methods Eight cases of solitary renal staghorn calculi associated with renal insufficiency were treated by a combination of MPCNL and ESWL. Results Stones were completely cleared away in 5 cases, while residual stones were found in 3 cases. No blood transfusion was required and no severe surgery-related complications were encountered. After surgery variable degrees of improvement in renal functions was observed. The serum creatinine (Cr) decreased from 289?166 ?mol/L pre-operation to 155?33 ?mol/L post-operation (t=4.69, P=0.004), and the blood urea nitrogen (BUN) decreased from 15.1?7.9 mmol/L to 8.3?1.9 mmol/L (t=4.00, P=0.005). The emission computed tomography (ECT) examinations showed the glomerular filtration rate (GFR) was elevated from 48.8?12.4 ml/s before operation to 63.0?8.4 ml/s after operation (t=4.68, P=0.003). Post-renal obstruction disappeared after operation. Follow-up for 0.5~4.5 years (mean, 2.8 years) in the 8 cases revealed no obvious changes in renal functions. Conclusions Combination of MPCNL and ESWL for solitary renal staghorn calculi associated with renal insufficiency is safe and effective.

Objective To optimize the prescription and technology of Brucea javanica oil subnanoemulsion injection. Methods Using superfine Brucea javanica oil as oil phase,yolk lecithin and poloxamer 188 as the complex emulsifying agents. The dosage of oil,emulsifier and stabilizer were optimized and the preparation was optimized by orthogonal experiments. Results The optimum precription was 10% oil phase,1.2% emulsifier and 0.1% stabilizer. The optimum technology was that aqueous phase and oil phase mixed at 70 ℃,sheared 20 min by high speed shearing machine and then passed the ultra-high pressure nano homogenizer 6 times under the pressure of 900 bar. Conclusion The physical stability of Brucea javanica oil subnanoemulsion injection is good.

Objective To investigate the treatment of Navicular bone fracture with Herbert screw.Methods Twenty-three patients with Navicular bone fracture were collected.They were all conducted with CT scan diagnosis and type,the small incision and targeting wire was used in operation.The Herbert screw was used to fix with pressure,plaster was also used to fix.After operation,the activity training was executed gradually.Results We conducted follow-up of 6-18 months in 23 patients,with an average of 12 months.All patients got bone-healing,the healing time ranges from 7 to 10 weeks,with an average of 9 weeks.There was one case of screw penetrating cortical bone,and one case of mild deformity healing,among the other 23 cases there were no complications including infection,nonunion,necrosis,osteoarthritis'etc.Conclusion The treatment of Navicular bone fracture with Herbert screw fixation can get firm fixation,function training and healing early,and it's worth of clinical promotion.

0.05).RGR showed that the toxicity level of magnesium alloys was 0.And there was no significant abnormality found in biochemical indicators and pathological analysis after inplant in vivo.[Conclusion]Magnesium alloys showed good biocompatibility to L929 mouse fibroblasts,and had no cacoethic effect to test animals,so the magnesium alloys is possible to be a new type and potential of bone surgery implant materials.

[Objective]To observe mouse osteoblasts vitality,histomorphology and growing development in vitro co-culture combined with magnesium alloys,confirm the biocompatibility of magnesium alloys and osteoblasts,and try to find out the possibility of magnesium alloys to be a new type of bone surgery implant material.[Method]Mouse osteoblasts were checked by Gomori and Von kossa stained,cultured and developed in vitro.Then the osteoblasts were mixed with magnesium alloys in a cell density of 3?104/ml.After 24、48、72 hours co-culture,the surface of magnesium alloys were observed by scanning electron microscopy(SEM) and confocal microscopy to find the change of osteoblasts.[Result]After culture in vitro,the osteoblasts well developed,and expressed stable character.After co-culture with magnesium alloys,the cells adhered and proliferated on the surface of alloys very well from SEM and confocal microscopy observation,which showed the osteoblasts had great activity and reproduce capability.[Conclusion]Magnesium alloys showed good biocompatibility and bone conduction capability with mouse osteoblasts,so the magnesium alloy is very possible to be a new type of bone surgery implant material.