The organophosphorus insecticides including a large group of insecticides were widely used in the world in the agriculture and household. Over 80% of the pesticides used in China are organophosphorus pesticides. Workers and the general population may be exposed to insecticides mixtures through water,air and the residue of food. After entering into human body,the insecticide mixtures can cause a combined toxicity. In this paper,the effects and toxicity mechanisms of combined exposures on health were expounded.

Objective To observe the influence of Dunhuang Nourishing Cream on the contents of SOD, GSH-PX and MDA in skin of aged rats. Methods Female SD rats of SPF level were divided into 6 groups:high, moderate and low dose groups of Dunhuang Nourishing Cream, model contrasting group, positive contrasting group and blank contrasting group. After removed the hair on the back, the drug groups were applied with Dunhuang Nourishing Cream, the model group was applied with the matrix of Dunhuang Nourishing Cream and the positive group was applied with vitamin E. After 30 days, the contents of SOD, GSH-PX and MDA in the skin were tested with the method of biochemistry analysis. Results Compared with the model group, the contents of SOD, GSH-PX and MDA in the high, moderate and low dose groups had significant difference (P

The WAN Mi-zhai's health preserving thought includes guayu,shendong,fashi and queji .The key of guayu is to control appetition to conserving essence.The aim of shendong is to keep the good psychology with peaceable mind.The view-point of fashi is to keep up with nature for harmonizing yin and yang.The denotation of queji is to prevent disease and regulate essence, qi and spirit.

Objective To investigate the clinical efficacy and safety of laparoscopic appendectomy for acute appendicitis.Methods Nighty-two cases of acute appendicitis were included in this study,randomly divided into observation group (n =46) and control group (n =46) according to the random number table method.The observation group was treated with laparoscopic appendectomy,and control group with the traditional open appendectomy treatment.We compared the two groups of cases in postoperative conditions and the level of WBC changes and 1d and 3d after operation.Results The operation time of the observation group was shorter than that of the control group (t=4.049,P＜0.05);there was no significant difference in lymph nodes (t=0.570,P＞0.05);the amount of bleeding was less than that of the control group (t=20.748,P＜0.05).The get-out-of-bed activity time in the observation group was faster than the control group (t=9.221,P＜0.05);eating time was faster than the control group (t=8.794,P＜0.05);the anus recovery exhaust time was faster than the control group (t=17.423,P＜0.05);hospital stay was shorter than the control group (t=13.479,P＜0.05);the complication rate was lower than that of the control group (t=13.883,P＜0.05).The levels of 1d and 3d WBC were lower in the observation group than that in the preoperative group (t=7.277,26.911,P＜0.05);the levels of 1d and 3d WBC in the observation group were lower than those in the control group at the same time (t=3.6189,17.827,P＜0.05);the 3d WBC level of the observation group was lower than that of 1d (t=20.732,P＜0.05).Conclusion Laparoscopic appendectomy is a safe and effective treatment for patients with acute appendicitis,and it is worthy of further clinical research.

ObjectiveTo study the relationship between the range of operations and the incidence of climacteric syndrom,the time of episode, degree and estrogen level in endometriosis after operations. MethodsThesexual hormone level was measured and climacteric syndrome was followed up after operation in 55 endometriosis. ResultsThe incidence of climacteric syndrom in oophorectomy was more frequent and earlier than partial oophorectomy. Meanwhile, the syndrome was much severe and the level of E2 was lower in the former group than those in the later one. ConclusionSo we should retain the ovary tissue and blood supply as much as possible. 6 months postoperation is suggested to be suitable to begin hormone replacement therapy. The dosage should be adviced to remain the blood hormone level just like one ovary does.

0.05).RGR showed that the toxicity level of magnesium alloys was 0.And there was no significant abnormality found in biochemical indicators and pathological analysis after inplant in vivo.[Conclusion]Magnesium alloys showed good biocompatibility to L929 mouse fibroblasts,and had no cacoethic effect to test animals,so the magnesium alloys is possible to be a new type and potential of bone surgery implant materials.

[Objective]To observe mouse osteoblasts vitality,histomorphology and growing development in vitro co-culture combined with magnesium alloys,confirm the biocompatibility of magnesium alloys and osteoblasts,and try to find out the possibility of magnesium alloys to be a new type of bone surgery implant material.[Method]Mouse osteoblasts were checked by Gomori and Von kossa stained,cultured and developed in vitro.Then the osteoblasts were mixed with magnesium alloys in a cell density of 3?104/ml.After 24、48、72 hours co-culture,the surface of magnesium alloys were observed by scanning electron microscopy(SEM) and confocal microscopy to find the change of osteoblasts.[Result]After culture in vitro,the osteoblasts well developed,and expressed stable character.After co-culture with magnesium alloys,the cells adhered and proliferated on the surface of alloys very well from SEM and confocal microscopy observation,which showed the osteoblasts had great activity and reproduce capability.[Conclusion]Magnesium alloys showed good biocompatibility and bone conduction capability with mouse osteoblasts,so the magnesium alloy is very possible to be a new type of bone surgery implant material.

[Objective]To study the mechanism of magnesium alloy degradation and bone formation at the bone-implant interface after implanting a magnesium alloy into rat femur.[Method]SD rats femur were filled with magnesium alloy stick.Nine weeks later,animals were killed and femur were retrieved.Systematic investigations on the surface morphology,composition,structure of bone-implant were performed by means of metallurgical microscope,scanning electron microscopy(SEM),and energy dispersive spectrum(EDS).Decalcified sections were prepared,and histologic examination was carried out.[Result]The bone response happened both on the surface of surrounding bone and the surface of magnesium alloy degradation layer while magnesium alloy degradating in rat femur.It was formed three layers at the interface of bone-implant: the metal layer,the degradation layer,and the new bone layer.Discontinuity connective tissue could be seen on the new bone layer but no inflammatory cells were found.[Conclusion]The new bone response at the interface of bone-implant is consistent with normal bone tissue.Magnesium alloys have good characters of degradation ability,osteogenesis ability,and histocompatibility.And the rate of degradation is corresponding to the rate of new bone formation.

Objective: To search for the indications of broncho-angioplastic pulmonary resection by comparing it with pnumonectomy and lobectomy in patients with non-small cell lung cancer. Methods: Sixty-four patients with non-small cell lung cancer (TNM classification: T0 21, N1 28 and N2 15) underwent broncho-angioplastic pulmonary resection in our hospital from 1980 to 2000. The experience on the operative method and perioperative managements were summarized and the postoperative mortalities were compared between patients with different TNM classifications. Meanwhile, 64 non-small cell lung cancer patients receiving pneumonectomy and 64 receiving lobectomy during the same period were also included in this study, and their postoperative mortalities and complications were compared with those of broncho-angioplastic pulmonary resection. Results: The 3, 5 and 10-year survival rates of patients receiving broncho-angioplastic pulmonary resection were 58%, 42% and 19%, respectively, with a medium survival time of 45.76 months. The 3, 5 and 10-year survival rates of 21 N0 patients were 82%, 56% and 29%, respectively; of 28 N1 patients were 56%, 39% and 27%, respectively; and of 18 N2 patients were 21%, 12% and 0, respectively. Significant difference was found between each TNM classification group in survival rates (P<0.01). The postoperative mortalities and complications in patients receiving broncho-angioplastic pulmonary resection were similitar with those of pneumonectomy, but significantly higher than those of lobectomy (P<0.01). Conclusion: Broncho-angioplastic pulmonary resection expands the indication spectrum of the lung cancer and improves the post-operation life quality of patients. Broncho-angioplastic pulmonary resection is suitable for patients with lung cancer of N0 or N1 status, but not recommendable for N2 status.

Objective: To study the pharmacokinetics of phosphatidylserine (PS)-containing curcumin-loaded nanostructured lipid carriers (Cur-mNLC), curcumin-loaded nanostructured lipid carriers without PS (Cur-NLC), and curcumin solutions in SD rats. Methods: Blood samples were collected from the orbit of rats at different periods of time after they were ip injected with Cur-solution, Cur-NLC, and Cur-mNLC at 10 mg/kg (corresponding to Cur), then Cur content in plasma was determined by UPLC-MS/MS, and the pharmacokinetic parameters were calculated by DAS software. Results: The pharmacokinetic parameters of Cur-solution, Cur-NLC, and Cur-mNLC were respectively calculated as follows: Cmax (29.453±1.146), (20.045±0.818), (15.865±0.409) μg/L; MRT(0-∞) (18.196±1.456), (18.196±1.456), (89.252±12.049) h. The AUC(0-∞) of Cur-mNLC was 2.58 and 1.48 times larger than those of Cur-solution and Cur-NLC. Conclusion: The Cmax of Cur-NLC and Cur-mNLC are lower than that of Cur-solution, but the MRT(0-∞) of Cur-NLC and Cur-mNLC are longer than that of Cur-solution. Compared with Cur-NLC, Cur-mNLC has better sustained-release behaviors, which contributes to its superior bioavailability.