Objective To investigate the influence of chiropractic therapy on the growth and intellectual development of 6 months infants.Methods 160 cases of 1-month-old full-term normal infants were divided into the chiropractic therapy group and the control group,each group contained 80 samples.Chiropractic therapy was given to the chiropractic therapy group daily.The development of weight,length,head circumference were observed,and the intelligent development for two groups were evaluated at the age of 6 months.Results The weight,body length in the chiropractic therapy group at the sixth month were higher than those in the control group.The intellectual development in the chiropractic therapy group was also higher than that in the control group.Conclusions Chiropractic therapy plays a significant role in promoting the physical and mental development of babies of 6 months,which is worth being promoted.

Objective To discuss the discovery of the neurbehavioral examination to premature infants in early abnormal neurobehavior and evaluate the influence of the hospital and family intervention pattern on the growth and development of the premature infants,so as to provide reference to the abnormal test of the premature infants and discuss the feasibility of developing neurobehavioral examination widely.Methods 152 cases of the intervention group were given neurobehavioral measurement after correction age,when the score ＜37 points,intervention and treatment were suggested in regular hospital (including exercise therapy and physical therapy,electrical nerve stmulation with treatment instrument,etc.).When the score ≥ 37points,personalized training plans were provided at home,which was adjusted in the follow-up.138 cases of the control group were given development assessment and neurobehavioral examination 3 months after correcting of age.Results The abnormal detection rate of the nervous system in the control group was higher than that of the intervention group,the scores of development in the control group were lower than that of the intervention group.Conclusions NBNA check is simple,safe and strong,it can reduce the occurrence of abnormal nervous system,and improve the level of intellectual and motor development.

Objective To study the effect of disodium quercetin-7,4′-disulfate (SQDS) on activity ofrecombinant human phosphoinositide 3-kinase (PI3-K) p110?catalytic subunit. Methods Recombinanthuman PI3-K p110?catalytic subunit was expressed by gene engineering. PI3-K activity was assayed byincubating recombinant PI3-K p110?with phosphatidylinositol-4,5-bisphosphate and [?-32P] ATP;the32P-radiolabeled lipids were extracted with chloroform and methanol,then assessed by scintillation counter.Results SQDS showed inhibition on the recombinant p110?catalytic subunit with IC5014.88?mol/L.Conclusion SQDS is an inhibitor of PI3-K. The recombinant PI3-K p110?catalytic subunit might be usedas a molecular target for simpler screening and development of more effective inhibitors of PI3-K.

Objective To explore the gene frequency of apolipoprotein A5(APOA5)-1131T>C single nucleotide polymorphisms and its relationship with the fat contents of adult Tibetans in Tibet and Han nationality population in Liaoning province.Methods In 100 Tibetan nationality(the study group) and 100 Han nationality individuals(the control group), the polymorphism of apoa5-1131T>C genotypes was detected by polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP), and fat contents were got by using bioelectrical impedance measurement.Results Results of fat contents of body, trunk and limbs(left upper limbs, left lower limbs, right upper limbs, right lower limbs and total limbs) were respectively below:① There was significant difference between male TC+CC genotype and TT genotype of Tibetan nationality.There was significant difference between male TT genotype of Tibetan nationality and of Han nationality.② There was significant difference between female TT genetype of Tibetan nationality and of Han nationality except result of the body fat contents.③ There was significant difference between the trunk and total limbs fat contents in male TT genotype, and male TC+CC genotype of Tibetan nationality and of Han nationality, and female TT genotype of Tibetan nationality.Conclusion APOA5 gene-1131T>C affects the body fat contents of Tibetans and Han nationality population, and APOA5 gene-1131T>C probably affects lipopexia in trunk.

OBJECTIVE To evaluate the efficacyand safety of intratympanic injection of steroids in the treatment of sudden hearing loss with diabetes.METHODS PubMed, Medline, CNKI, CBM, VIP, and Wanfang databases were searched by electronic retrieval to collect documents meeting the inclusion criteria and extract data.Meta-analysis was performed using RevMan 5.3.RESULTS There were 9 articles included in the literature search,a total number of 587 ears, of which 294 ears accepted intratympanic injection of steroids as the experimental group. Another 293 ears was control group,of which 217 ears accepted systemic steroids and 76 ears accepted non-hormone therapy.Meta analysis results showed that the clinical effect of experimental group was more remarkable than that of the control group (Z=3.65, P<0.05;Z=3.70,P<0.05). The influence on blood sugar in experimental group was smaller than that of the control group (χ2=7.471,P<0.05). Other complications of intratympanic steroid injection were self-healing after tympanic membrane perforation, transient dizziness and so on.CONCLUSION There was a significant effect on patients with sudden hearing loss and diabetes through intratympanic steroid injection. It has little effect on blood glucose and slight complications, which is a priority in clinical practice.

Rheumatoid arthritis is a autoimmune diseases characterized as joint chronic synovitis, and is accompanied with systemic damage.Osteoporotic fracture is one of the common complications of rheumatoid arthritis, which may decrease quality of life, and even increase the mortality of patients.Variety of factors may be associated with the occurrence of osteoporotic fractures in rheumatoid arthritis patients.In this article, through the literature review we discuss the risk factors of osteoporotic fracture, including vitamin D deficiency, lower limb muscle strength and balance ability in rheumatoid arthritis patients.

Objective To study the clinical significance of sernm CA153,CA125 and CEA test in breast cancer.Methods The levels of CA153,CA125,carcinoembryonic antigen(CEA)and ferritin were measured in 60 patients with breast cancer(breast cancer group),36 patients with benign breast diseases(benign breast diseases group)and 40 healthy people(control group)by chemiluminometry.The four indices were compared and analyzed for their complementary diagnostic value to breast cancer.Results The levels of CA153,CA125,CEA,SF[(52.8±21.3)u/ml,(44.2±20.1)ng/ml,(8.9±5.2)ng/ml,(350.5±113.8)ng/ml]in breast cancer group were significantly higher than that of benign breast disease group[(17.3±8.8)u/ml,(15.6±8.5)u/ml,(2.0±0.8)u/ml,(1220.7±46.91)ng/ml](t=2.671,t=2.684,t=2.898,t=2.844,P＜0.01);The levels of CA153,CA125,CEA and SF pretreatment in breast cancer group were significantly higher than that after treatment[(25.5±3.7)u/ml,(15.0±8.4)u/ml,(4.6±3.3)ng/ml,(98.5±58.6)ng/ml](t=2.210,t=2.165,t=2.224,t=2.234,P＜0.05);The positive rate of 53.3%in breast cancer group for CA153+CA125+CEA+SF were lower than that CA153(56.7%),CA125(58.3%),CEA(63.3%),SF(68.3%)(χ~2=2.52,χ~2=2.652,P＞0.05;χ~2=3.85,χ~2=3.90,χ~2=3.98,P＜0.05);joint determination of CA153+CEA+SF experimental efficient 89.0%higher than the other four groups of the joint determination,but had no signiflcanle(χ~2=2.78,χ~2=3.10,χ~2=2.99,χ~2=3.01,P＞0.05).Conclusion The positive rate may be increased by combining test of serum CA153,CA125,CEA and ferritin in breast cancer.Thus the combined test might be of high value for the early diagnosis,improving the therapeutic effect and prognosis of breast cancer.

Aim To study the effect of sodium quercetin-7-sulfate (SQMS) on activity of recombinant human phosphoinositide 3-kinase (PI3-K) p110? catalytic subunit.Methods Recombinant human PI3-K p110? catalytic subunit was expressed by gene engineering.PI3-K activity was assayed by incubating recombinant PI3-K p110? with phosphatidylinositol-4,5-bisphosphate and [?-~ ~32 P]ATP; the ~ ~32 P-radiolabeled lipids were extracted with chloroform and methanol, and assessed by scintillation counter.Results SQMS showed inhibition on the recombinant p110? catalytic subunit with IC_~50 13.14 ?mol?L~-1 .Conclusions SQMS is an inhibitor of PI3-K. The recombinant PI3-K p110? catalytic subunit might be used as a molecular target for simpler target screening more effective inhibitors of PI3-K.

Objective To investigate the morphology and bonding of enamel and dentin conditioned by 35% phosphoric acid and pulse Nd:YAG laser.Methods A total of 52 human premolars were selected,40 of which were divided into four groups randomly: group A [enamel treated with 35% phosphoric acid(Scotchbond,3M) for 30 s];group B [enamel treated with pulse Nd:YAG laser(Evlaser Friendly A4 Italy) for 20 s];group C(dentin treated with 35% phosphoric acid for 15 s);group D(enamel treated with pulse Nd:YAG laser for 15 s).The tensile bond strength(TBS) were determined.Except 4 out of 12 human premolars without any treatment,8 were divided into 4 groups and were treated with the method mentioned above.The surfaces of 12 human premolars were observed under SEM.Results The highest TBS was obtained in group A in comparison with the other 3 groups(P0.05).The smear layer on enamel and dentin could be removed after conditioned by both phosphoric acid and pulse Nd:YAG laser.The honeycomb structures were observed on all enamel but less regularly in lased group.Almost all the orifices of dentinal tubules were open in the acid group and sealed or half-sealed in lased group.Conclusion The pulse Nd:YAG laser did not contribute to bonding of enamel,but could improve the bonding of dentin and seal most of the orifices of dentinal tubules,which reduced the harmful stimulation to the dental pulp.