2.Comparative Study on Polytene Chromosomes of Two Isolates of Simulium quinquestriatum
Xiaojun WEN ; Jing WEI ; Hanbing CHEN
Chinese Journal of Parasitology and Parasitic Diseases 1987;0(03):-
The salivary glands were exposed and isolated from the larvae of Simulium quinquestriatum and stained in carbol fuchsin, squashed between slide and coverslide. Slides were examined and photographed under microscope to measure the polytene chromosomes. Systematic analysis was made. Results indicated that the number of the polytene chromosomes of both isolates is three. The main characteristic chromosomal structures are homologized. Only the banding types of ⅡL are different.
3.Satisfactory investigation of bandage contact lens on patients after pterygium surgery
Chinese Journal of Practical Nursing 2016;32(3):196-199
Objective To evaluate the clinical effect of bandage contact lens on patients after the pterygium excision surgery.Methods 200 patients with pterygium excision were used retrospectively analysis and were divided into control group and observation group by random digital table method.Patients in observation group wore bandage contact lens while those in control group did not (traditional method).The degree of pain in both groups was evaluated at 8 hours,24 hours and 48 hours after surgery.In addition,patients were surveyed for the satisfaction of our treatment when discharge.Results The ocular foreign body sensation,eye burning,tears and eye open difficult were obviously decreased in observation group (t=5.643,10.315,P < 0.01).The overall comfort level of the patients in observation group was obviously improved.pain feeling of the eye after surgery in 8h,24h and 48h markedly decreased in observation group (t=6.196-12.495,P < 0.01).The degree of satisfaction scores of preoperative guidancein,postoperative pain control,emotion,mental nursing,self-care ability,comfort in observation group were (1.59-0.62) points,(1.83±0.70) points,(1.42±0.68) points,(1.42±0.68) points,(1.48±0.67) points,(1.29±0.77) points,(1.43-0.64)points which were obviously increased than (0.76±0.55) points,(0.83±0.64points,(0.82±0.56) points,(0.80±0.47) points,(0.79±0.61) points,(0.66±0.54) points (t=4.311-9.354,all P< 0.01).Conclusions Compared with traditional treatment,bandage contact lens can significantly reduce the discomforts after the pterygium excision surgery,and increase the postoperative comforts of patients.It proves that bandage contact lens have extensive clinical value.
4.Understanding of obesity pathogenesis from human energy metabolism evolution perspective
Jing WU ; Hong-Wei WANG ; Yu WEN ;
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
This article elucidates the relationship between the human susceptibility to obesity and gene polymorphisms such as peroxisome proliferators-activated receptors(PPARs)and PPAR?coactivator-1,along with milestones in the formation and development of capacity for fat deposition during evolutionary history of human.An biological evolutionary analysis,identifying factors favoring the energy stores,may be helpful to the development of preventive public health strategies.
5.Effect of hyperoxic liquid on gastric intramucosal pH after myocardial ischemic reperfusion injury in rabbits
Wei WANG ; Jian WEN ; Guixia JING
Journal of Xi'an Jiaotong University(Medical Sciences) 2003;0(06):-
Objective To investigate the changes in gastric intramucosal pH(pHi) and the effect of hyperoxic liquid on intramucosal pH(pHi) after myocardial ischemia-reperfusion injury in rabbits.Methods Twenty healthy male adult rabbits,weighing 2.3-3.0 kg,were randomly divided into two groups: control group(n=10) and hyperoxic liquid group(n=10).Hyperoxic liquid group received the routine infusion of hyperoxic liquidle of 10 mL/kg for seven days,while control group received the routine treatment infusion of saline instead of hyperoxic liquid of 10 mL/kg.Seven days later,the myocardial ischemic reperfusion model(I/R) was established,and ECG and homodynamic indexes were monitored simultaneously.Hemodynamic indexes and pHi were measured before myocardial ischemia,30 min after myocardial ischemia as well as 1 h and 2 h after reperfusion.Results Hyperoxic liquid could lower ST segment and improve hemodynamic indexes.pHi decreased significantly after myocardial ischemia-reperfusion injury in both groups.Compared with that in control group,pHi was significantly higher at 1 h and 2 h after reperfusion in hyperoxic liquid group(P
6.The protective effect of hyperoxic liquid preconditioning in myocardial ischemia-reperfusion injury in rabbits
Guixia JING ; Jian WEN ; Wei WANG
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
Objective To study the protective effect of hyperoxic liquid preconditioning in myocardial ischemia-reperfusion injury. Methods Thirty rabbits were randomly divided into three groups: group A undergoing all procedures except ischemia-reperfusion, group C receiving the routine infusion of hyperoxic liquid 15mL/kg for seven days, and group B receiving the routine treatment infusion of saline instead of hyperoxic liquid 15mL/kg. Seven days later, the myocardial ischemic reperfusion model (I/R) was fabricated, ECG and homodynamic index were monitored simultaneously. Plasma MDA and SOD level was assayed before ligation, 30min after ligation and 1, 2, 3h after reperfusion. Results Hyperoxic liquid preconditioning can lower ST segment and improve index of hemodynamics and significantly reduce myocardial infarction size in group C compared with group B((P
9.Relationship between GABAA receptor and the analgesic、hypnotic and amnesic effects induced by GABA
Wei CHENG ; Jing WEN ; Tijun DAI ; Yong JI
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To investigate the effect of the GABAA receptor on the analgesia,hypnosis and amnesia induced by GABA in mice.Methods GABA was intracerebroventricularly(icv) injected and securinine,a GABAA receptor antagonist,was intravenously(iv) injected after the GABA intracerebroventricularly injection.Then four techniques including hot-plate test,acetic acid-induced writhing test,awaken test as well as step-through test & step-down test were employed to evaluate the effects on the pain index in hot-plate test(HPPI),the writhing times,the sleeping time(ST) and the latency & the number of errors.Results GABA increased the HPPI and ST,but decreased writhing times and the latency & increased the number of errors in conscious mice;the above effects are dose-dependent.Securinine could antagonize the effects stimulated by GABA.Conclusion GABA lead to analgesia,hypnosis and amnesia.GABAA receptor might be the important target for these effects.
10.Blood supply analysis based on quantitative dynamic contrast-enhanced MRI in hepatocellular carcinoma
Jing WEI ; Liguang ZOU ; Cuiwei LIAO ; Li WEN
Journal of Third Military Medical University 1984;0(01):-
Objective To analyze the status of blood supply at appearance of the first enhanced signal on dynamic contrast-enhanced MRI of hepatic cell cancer(HCC) of differentiated degree.Methods HCC was induced chronically with 1 ml of 2% DEN solution once a day in 80 rats for 14 weeks,and another 10 rats served as normal controls.All rats received dynamic contrast-enhanced MRI examination on week 16,18,20,21,22,23,24,25.The time-intensity curve was drawn and the slope rate of the first enhanced signal(S_(first)) was counted,then specimens were taken and observed of microvessel density(MVD) with light microscope.Results The differentiated degree of HCC was correlated with S_(first) and MVD.The poorer HCC differentiation,the bigger S_(first).Well differentiated HCC had low MVD,while poorly differentiated had higher MVD.Conclusion MVD is the main influence factor for that the S_(first) were significantly different between HCC of differentiated degree.