1.Recent advances in pathology and molecular genetics of small cell carcinoma of the urinary bladder.
Liang CHENG ; Wen-bin HUANG ; Jie CHEN
Chinese Journal of Pathology 2007;36(10):700-703
Biomarkers, Tumor
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metabolism
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Carcinoma, Small Cell
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genetics
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metabolism
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pathology
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Carcinoma, Squamous Cell
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pathology
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Chromosome Aberrations
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Diagnosis, Differential
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Humans
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Keratins
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metabolism
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Lymphoma
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pathology
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Mucin-1
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metabolism
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Urinary Bladder
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pathology
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Urinary Bladder Neoplasms
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genetics
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metabolism
;
pathology
2.The dynamic observation of the levels of IL-2,IL-5 and IL-6 produced by Balb/c mice infected with Dengue Virus type Ⅱ clinic strain
Yu PAN ; Li ZUO ; Wen-Jie CHEN ;
Chinese Journal of Infectious Diseases 1997;0(04):-
Objective To observe the dynamic levels of IL-2,IL-5 and IL-6 produced by Balb/ c mice infected with DEN_2 clinical strains and to study their relation.Methods The Balb/c mouse in- feetion model was established by multiple-site subcutaneous injection with various doses of DEN_2 clini- cal strain.Mouse plasma samples collected from different experiment groups at various time after in fection were tested for IL-2,IL-5 and IL-6 levels with sandwich ELISA.Results After primary in- feeted with DEN_2 B strain,the levels of IL-2,IL-5 and IL-6 of all experimental groups were not sig- nificantly higher than the normal control group while the levels of experimental groups increased sig- nificantly after re-infection.The level of IL-2 reached to peak[average value of(101 522.44?10 465.375)pg/ml]at the 4th day after re-infection(the 20th day after the primary infection),and then the level gradually reduced.The levels of IL-5 in the Balb/c mice of the group 1 and 2 reached to peak at the 1st day after re infection(the 16th day after the primary infection),and there was signifi- cant difference between these two groups and the control group(P<0.05).The levels of IL-6 in all experimental groups reached to peak at the 1st and the 2nd day after re-infection.The peak value of the third group is the highest comparing with the normal control group(P<0.05).Conclusion Th2 response was predominant in the second infection phase.
6.Value of electronic colonoscopy in the diagnosis of childhood chronic diarrhea.
Jie-Yu YOU ; Chan-Bin CHEN ; Wen-Xian OU-YANG
Chinese Journal of Contemporary Pediatrics 2007;9(5):493-494
Adolescent
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Child
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Child, Preschool
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Chronic Disease
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Colonoscopy
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methods
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Diarrhea
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diagnosis
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pathology
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Female
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Humans
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Infant
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Infant, Newborn
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Male
7.Effect of isoflurane preconditioning on the nuclear factor kappa B activity of leukocytes in patients undergoing cardiac valve replacement with cardiopulmonary bypass
Wen-Jie LIU ; De-Quan CAO ; Yan-Ping CHEN ;
Chinese Journal of Anesthesiology 1994;0(06):-
Objective To investigate the effect of isoflurane preconditioning on the nuclear factor kappa B (NF-kB)activity of leukocytes in patients undergoing cardiac valve replacement with cardiopulmonary bypass (CPB).Methods Twenty ASAⅡorⅢpatients of both sexes aged 20-60 yrs undergoing cardiac valve replacement under CPB were randomly divided into 2 groups(n=10 each):control group(C)and isoflurane preconditioning group(I).Anesthesia was induced with midazolam 0.08-0.12 mg?kg~(-1),fentanyl 5-10?g?kg~(-1) and vecuronium 0.1 mg?kg~(-1).The patients were mechanically ventilated(FiO_2=100%)after tracheal intubation.Anesthesia was maintained with intermittent i.v.boluses of fentanyl and midazolam in group C or with 2 MAC isoflurane and intermittent i.v.boluses of fentanyl and midazolam in group I before CPB.Systolic BP was kept between 90-120 mm Hg,diastolic BP between 50-80 mm Hg and HR between 60-90 bpm in both groups. Isoflurane was discontinued at the initiation of CPB.Arterial blood samples were taken after tracheal intubation and before inhalation of isoflurane(T_0)at 30 min(T_1),1 h(T_2)and 2 h(T_3)after aortic unclamping for determination of NF-kB activity of leukocytes using electrophoretic mobility shift assay(EMSA).The amount of fentanyl,midazolam,dopamine and sodium nitroprusside(SNP)consumed during operation and the rate of recovery of spontaneous heart beat in both groups were recorded.Results The NF-kB activity was significantly increased after aortic unclamping in C group but there was no significant difference in NF-kB activity before CPB (T_0)and after aortic unclamping(T_(1-3))in I group.The NF-kB activity was significant lower at T_(1-3) in group I than in group C.The total amount of fentanyl consumed was 40-60?g?kg~(-1) in C group and 20-30?g?kg~(-1) in group I. Significantly less amount of dopamine was used in group I than in group C.There was no significant difference in SNP consumption between the 2 groups.The rate of recovery of spontaneous heart beat was significantly higher in group I than in group C(P<0.01).The amount of dopamine consumed was positively correlated with the highest level of NF-kB activity in both group[r=0.962 in group C;r=0.908 in group I(P<0.01)].Conclusion Isoflurane preconditioning can attenuate the NF-kB activity of leulocytes in patients undergoing cardiac valve replacement under CPB.
8.Effect of two anesthesia ways on short-term cognitive function of elderly patients with lacunar infarction after surgery
Ying ZHANG ; Jie CHEN ; Xinrong WEN ; Kaizhi LU
Journal of Regional Anatomy and Operative Surgery 2016;25(11):841-843
Objective To investigate the influence of combined spinal-epidural anesthesia and general anesthesia on short-term cogni-tive function of elderly patients with lacunar infarction after surgery.Methods A total of 50 patients with lacunar infarction who underwent abdominal surgery in our hospital from June 2012 to December 2013 were selected as the research object,who were divided into spinal-epi-dural anesthesia group (combined group)and general anesthesia group (general group).The incidence of postoperative cognitive dysfunction of two groups were observed and compared.The mini-mental state examination(MMSE)and Montreal cognitive Assessment(MoCA)were used to evaluated the cognitive function before and postoperative 1 day.Results The probability of postoperative cognitive dysfunction (POCD)of combined group and the general group were 12% and 32%,respectively,and the POCD probability of combined group was lower than that of general group,the difference was significant(P <0.05).The MMSE score and MoCA score at postoperative 1 day of two groups were lower than those before anesthesia,the difference was significant(P <0.05).The MMSE score and MoCA score of combined group at postoperative 1 day were lower than that of general group,the difference was significant(P <0.05).Conclusion The anesthesia can cause a certain cognitive dysfunction for elderly patients with lacunar infarction,while the spinal-epidural anesthesia can reduce the incidence rate of POCD compared anesthesia.
9.Effect of Xinnaojia coumfound on learning and memory and expression of NR2B in the hippocampus of rats with chronic alcoholism.
Li SHUANG ; Jia WAN ; Wen-Jie CHEN ; Guang-Rui WAN
Chinese Journal of Applied Physiology 2011;27(1):5-80
Alcoholism
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drug therapy
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metabolism
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physiopathology
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Animals
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Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Hippocampus
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metabolism
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Learning
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drug effects
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physiology
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Male
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Memory
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drug effects
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physiology
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Phytotherapy
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RNA, Messenger
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genetics
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metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, N-Methyl-D-Aspartate
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genetics
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metabolism
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Superoxide Dismutase
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metabolism
10.Bony ingrowth induced by treadmill exercise in rats with femoral shaft bone defects
Jie ZHANG ; Wen ZHANG ; Xi CHEN ; Zongping LUO ; Huilin YANG
Chinese Journal of Tissue Engineering Research 2014;(15):2320-2325
BACKGROUND:It is wel-known that mechanical stimulation could promote fracture healing. However, what kind of mechanical stimulation induced by treadmil exercise can increase the bone conductibility of bone material and promote the healing of bone defect is stil unclear.
OBJECTIVE:To evaluate the influence of indirect mechanical stimulation produced by treadmil exercise on bone defect healing and osteogenesis of bone materials.
METHODS:Sprague-Dawley rats at 12 weeks old were used in this study to establish a bone defect of 3 mm in diameter and height at the left distal femur. Afterwards, calcium sulphate scaffolds were implanted into the defects. The rats were divided into treadmil exercise group and control group. Treadmil exercise was began at 1 week postoperatively, 10 m/min, 45 minutes per day, 5 days per week, for 3 weeks. Control group did not receive any exercise. Micro-computed tomography was used to determine bone formation in the bone defects at 1, 2, 3, and 4 weeks after surgery. The sections of left distal femur were subject to hematoxylin-eosin staining, the new bone formation and degradation of bone materials in the bone defects were observed.
RESULTS AND CONCLUSION:Micro-CT analysis showed that, a smal amount of new bone formed in both treadmil exercise group and control group at 1 week after surgery. In treadmil exercise group, new bone formation was significantly higher than the control group at 2, 3, 4 weeks (P<0.05). At 4 weeks, histological results also confirmed the difference of new bone formation in bone defect between treadmil exercise group and control group. In addition, bone mineral density of treadmil exercise group was higher than that of control group at 2, 3, 4 weeks, but no significant difference was found (P>0.05). The results suggest that moderate treadmil exercise could promote bone defect healing and enhance osteoconductivity of bone substitute.