1.Recent advances in pathology and molecular genetics of small cell carcinoma of the urinary bladder.
Liang CHENG ; Wen-bin HUANG ; Jie CHEN
Chinese Journal of Pathology 2007;36(10):700-703
Biomarkers, Tumor
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metabolism
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Carcinoma, Small Cell
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genetics
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metabolism
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pathology
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Carcinoma, Squamous Cell
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pathology
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Chromosome Aberrations
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Diagnosis, Differential
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Humans
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Keratins
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metabolism
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Lymphoma
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pathology
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Mucin-1
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metabolism
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Urinary Bladder
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pathology
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Urinary Bladder Neoplasms
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genetics
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metabolism
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pathology
2.The dynamic observation of the levels of IL-2,IL-5 and IL-6 produced by Balb/c mice infected with Dengue Virus type Ⅱ clinic strain
Yu PAN ; Li ZUO ; Wen-Jie CHEN ;
Chinese Journal of Infectious Diseases 1997;0(04):-
Objective To observe the dynamic levels of IL-2,IL-5 and IL-6 produced by Balb/ c mice infected with DEN_2 clinical strains and to study their relation.Methods The Balb/c mouse in- feetion model was established by multiple-site subcutaneous injection with various doses of DEN_2 clini- cal strain.Mouse plasma samples collected from different experiment groups at various time after in fection were tested for IL-2,IL-5 and IL-6 levels with sandwich ELISA.Results After primary in- feeted with DEN_2 B strain,the levels of IL-2,IL-5 and IL-6 of all experimental groups were not sig- nificantly higher than the normal control group while the levels of experimental groups increased sig- nificantly after re-infection.The level of IL-2 reached to peak[average value of(101 522.44?10 465.375)pg/ml]at the 4th day after re-infection(the 20th day after the primary infection),and then the level gradually reduced.The levels of IL-5 in the Balb/c mice of the group 1 and 2 reached to peak at the 1st day after re infection(the 16th day after the primary infection),and there was signifi- cant difference between these two groups and the control group(P<0.05).The levels of IL-6 in all experimental groups reached to peak at the 1st and the 2nd day after re-infection.The peak value of the third group is the highest comparing with the normal control group(P<0.05).Conclusion Th2 response was predominant in the second infection phase.
3.Value of electronic colonoscopy in the diagnosis of childhood chronic diarrhea.
Jie-Yu YOU ; Chan-Bin CHEN ; Wen-Xian OU-YANG
Chinese Journal of Contemporary Pediatrics 2007;9(5):493-494
Adolescent
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Child
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Child, Preschool
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Chronic Disease
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Colonoscopy
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methods
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Diarrhea
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diagnosis
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pathology
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Female
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Humans
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Infant
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Infant, Newborn
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Male
5.Bony ingrowth induced by treadmill exercise in rats with femoral shaft bone defects
Jie ZHANG ; Wen ZHANG ; Xi CHEN ; Zongping LUO ; Huilin YANG
Chinese Journal of Tissue Engineering Research 2014;(15):2320-2325
BACKGROUND:It is wel-known that mechanical stimulation could promote fracture healing. However, what kind of mechanical stimulation induced by treadmil exercise can increase the bone conductibility of bone material and promote the healing of bone defect is stil unclear.
OBJECTIVE:To evaluate the influence of indirect mechanical stimulation produced by treadmil exercise on bone defect healing and osteogenesis of bone materials.
METHODS:Sprague-Dawley rats at 12 weeks old were used in this study to establish a bone defect of 3 mm in diameter and height at the left distal femur. Afterwards, calcium sulphate scaffolds were implanted into the defects. The rats were divided into treadmil exercise group and control group. Treadmil exercise was began at 1 week postoperatively, 10 m/min, 45 minutes per day, 5 days per week, for 3 weeks. Control group did not receive any exercise. Micro-computed tomography was used to determine bone formation in the bone defects at 1, 2, 3, and 4 weeks after surgery. The sections of left distal femur were subject to hematoxylin-eosin staining, the new bone formation and degradation of bone materials in the bone defects were observed.
RESULTS AND CONCLUSION:Micro-CT analysis showed that, a smal amount of new bone formed in both treadmil exercise group and control group at 1 week after surgery. In treadmil exercise group, new bone formation was significantly higher than the control group at 2, 3, 4 weeks (P<0.05). At 4 weeks, histological results also confirmed the difference of new bone formation in bone defect between treadmil exercise group and control group. In addition, bone mineral density of treadmil exercise group was higher than that of control group at 2, 3, 4 weeks, but no significant difference was found (P>0.05). The results suggest that moderate treadmil exercise could promote bone defect healing and enhance osteoconductivity of bone substitute.
8.Effect of Xinnaojia coumfound on learning and memory and expression of NR2B in the hippocampus of rats with chronic alcoholism.
Li SHUANG ; Jia WAN ; Wen-Jie CHEN ; Guang-Rui WAN
Chinese Journal of Applied Physiology 2011;27(1):5-80
Alcoholism
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drug therapy
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metabolism
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physiopathology
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Animals
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Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Hippocampus
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metabolism
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Learning
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drug effects
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physiology
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Male
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Memory
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drug effects
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physiology
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Phytotherapy
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RNA, Messenger
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genetics
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metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, N-Methyl-D-Aspartate
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genetics
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metabolism
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Superoxide Dismutase
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metabolism
9.The Construction of Human Cytomegalovirus pp65 Prokaryotic Expressing Vector and the Inducement of Specific Immune Response
Jie ZHAO ; Liangzhen WEN ; Suhua CHEN ; Al ET
Chinese Journal of Perinatal Medicine 1998;0(02):-
Objective To construct human cytomegalovirus pp65 prokaryotic expressing vector and induce specific T lymphocyte immune response with recombinant pp 65 protein of human cytomegalovirus(HCMV) to observe the effect of CTL on infected HEL cells. Methods The whole cDNA of HCMV pp65 was amplified by PCR and inserted into prokaryotic expressing vector pRSET by gene engineer technique. The product was purified by affinity chromphotography and identified with Western blot after that recombinant plasmid was expressed by the induction of IPTG. With MTT technique, we observed the stimulating and proliferating effect of recombinant HCMV pp65 protein on PBMC in vitro. Cytotoxicity of PBMC on HCMV-infected HEL cells was detected. Results The pp65 prokaryotic expressing vector was successfully constructed and could express in engineering bacteria DE3. High dose of pure recombinant protein was acquired and had been identified. The rhHCMV pp65 protein can activate PBMC and cause the proliferation of it in vitro. The proliferated PBMC have the specific cytotoxicity to HEL cells infected by HCMV. Conclusions The acquired recombinant HCMV pp65 protein could induce specific T cells immune response in vitro to kill the HCMV infected HEL cells. And it is very important for the immune therapy of the HCMV infections.
10.Effect of two anesthesia ways on short-term cognitive function of elderly patients with lacunar infarction after surgery
Ying ZHANG ; Jie CHEN ; Xinrong WEN ; Kaizhi LU
Journal of Regional Anatomy and Operative Surgery 2016;25(11):841-843
Objective To investigate the influence of combined spinal-epidural anesthesia and general anesthesia on short-term cogni-tive function of elderly patients with lacunar infarction after surgery.Methods A total of 50 patients with lacunar infarction who underwent abdominal surgery in our hospital from June 2012 to December 2013 were selected as the research object,who were divided into spinal-epi-dural anesthesia group (combined group)and general anesthesia group (general group).The incidence of postoperative cognitive dysfunction of two groups were observed and compared.The mini-mental state examination(MMSE)and Montreal cognitive Assessment(MoCA)were used to evaluated the cognitive function before and postoperative 1 day.Results The probability of postoperative cognitive dysfunction (POCD)of combined group and the general group were 12% and 32%,respectively,and the POCD probability of combined group was lower than that of general group,the difference was significant(P <0.05).The MMSE score and MoCA score at postoperative 1 day of two groups were lower than those before anesthesia,the difference was significant(P <0.05).The MMSE score and MoCA score of combined group at postoperative 1 day were lower than that of general group,the difference was significant(P <0.05).Conclusion The anesthesia can cause a certain cognitive dysfunction for elderly patients with lacunar infarction,while the spinal-epidural anesthesia can reduce the incidence rate of POCD compared anesthesia.