Dermatophagoid.es farinae allergen Ⅰ (Der f Ⅰ ) has been extracted and purified from spent culture medium of Dermatophagoid.es farinae by affinity chromatography coupled with anti-Der f Ⅰ McAb for the first time in China. Furthermore,its physicochemical properties were analysed. By SDS-PAGE,.Der f Ⅰ was estimated to have a molecular weight of 24 kDa and appeared as only one protein band. By isoelectric focusing ,it was revealed to have pIs of 4. 8 - 7. 5 with six protein bands,suggesting that Der f Ⅰ is heterogeneous with complicated composition.

Acupuncture Therapy ; Female ; Humans ; Middle Aged ; Syringomyelia ; therapy

Adolescent ; Adult ; Child ; Endoscopy ; Female ; Humans ; Male ; Maxillary Fractures ; surgery ; Middle Aged ; Nasal Surgical Procedures ; methods ; Young Adult

Objective To evaluate the effects and complications of percutaneous nephrolithotomy (PCNL) in the treatment of renal stone after repeated extracorporeal shockwave lithotripsy (ESWL).Methods Forty-four patients who had a history of repeated ESWL (treatment group) and 50 patients with-out surgical intervention (control group) were submited to PCNL,and clinical data was documented in details and analyzed.Results The time to establish access in treatment group and control group was (11.8 ± 4.1) min and (10.9 ± 2.5) min,respectively,and there was no significant difference (t =1.308,P =0.194).The time to extract stone in both groups was (92.0 ± 13.5) min and (66.6 ± 17.6) min,respectively,and there was significant difference (t =7.776,P =0.000).The operative time in treatment group was (113.9 ± 12.0) min,which was longer than that in control group with (87.6 ± 13.6) min (t =8.354,P =0.000).The clearance in both groups was 81.8％ and 94.0％,and there was no significant difference (x2 =3.361,P =0.067).The was no death or other severe complication in both groups.Conclusions The operation time in treatment group was longer than that in control group,and there was no significant difference in clearance and complication rate.Thus it was safe and effctive to perform PCNL in these patients with a history of failed repeated ESWL.

Child, Preschool ; Histoplasmosis ; complications ; pathology ; Humans ; Lymph Nodes ; pathology ; Male ; Mycobacterium Infections ; etiology

Heart Valve Diseases ; therapy ; Heart Valve Prosthesis Implantation ; methods ; Humans

Humans ; Occupational Health Physicians ; organization & administration ; Personnel Selection

Laboratory diagnostics is an important discipline functioning as a bridge between basic and clinical medicine and it is closely associated with the diagnosis of clinical physician.But there are some problems in the laboratory diagnostic teaching including unreasonable curriculum standard,simple teaching method and unpractical theory.This paper explored and summarized the problems and the reform of laboratory diagnostics teaching mode for students of clinical medicine.

Objective To study the effects of PTEN and missense mutations in PTEN phosphotase domain on AKT phosphorylation in AGS and BGC-823 cells. Methods The plasmids of wtPTEN,PTEN-C124S which PTEN mutant is in both lipid and protein phosphotase domain and PTEN-G129E which PTEN mutant is only in lipid phosphotase domain were respectively transfected into AGS and BGC-823 cells. The cells were stimulated with insulin or rhEGF after serum starvation overnight. The levels of AKT phosphorylation were detected by Western blot. Results Both insulin and rhEGF can activate AKT phosphorylation in gastric cancer cells. Overexpressed PTEN inhibitedAKT phosphorylation induced by insulin or rhEGF(P < 0.05). PTEN mutants C124S or G129E could not inhibitAKT phosphorylation(P > 0.05). Conclusions PTEN can inhibit AKT phosphorylation induced by insulin or rhEGF in gastric cancer cells. Missense mutations in the 124th or 129th amino acid of PTEN phospho-tase domain do not exert inhibitive function.

Objective To evaluate the application of blue on yellow(B/Y) perimetry in detecting the early visual field loss of glaucoma. [WT5”HZ]Methods [WT5”BZ]The B/Y perimetry of the G2 strategy in the OCTOPUS 101 perimetry was used to examine the visual field of 16 normal persons (32 eyes), 25 cases (35 eyes) of primary open angle glaucoma (POAG) with abnormal white on white (W/W) visual fields, 15 cases (15 eyes) of early POAG with normal W/W visual field, and 11 cases (22 eyes) of suspected POAG. [WT5”HZ]Results [WT5”BZ]The mean sensitivity (MS) in the normal cases, suspected POAG, early POAG, middle POAG, and late POAG was (23.24?1.22) dB,(20.97?3.42) dB,(18.44?3.57) dB,(11.04?1.85) dB and (8.55?2.29) dB, respectively. It was demonstrated that B/Y perimetry was more sensitive than W/W perimetry in detecting the glaucomatous visual field defects,and its sensitivity was 92% and specificity was 90.62%. The average number of defective points in central visual field with B/Y perimetry was more than that with W/W perimetry in early and middle POAG. Conclusion B/Y perimetry is a relatively sensitive method for detection of the early visual field loss in POAG.