This paper summarized professor YOU Zhaoling's experience in treating intrauterine adhesions （IUA） with medicinal flowers. According to the collateral disease theory， deficiency and stasis of uterus vessels and collaterals is the key pathogenesis of IUA. Medicinal flowers are mostly fragrant， light and soft， with the function of diffusing， unblocking， penetrating and dissipating， which can be used to diffuse and dissipate stasis and stagnation in the blood， and finally unblock the collaterals. In clinical practice， Renshenhua （Flos Ginseng）， Sanqihua （Flos Notoginseng）， Jinyinhua （Flos Lonicerae Japonicae）， Daidaihua （Flos Citri Aurantii Amarae）， Lyu'emei （Flos Mume）， Baihehua （Flos Bulbus Lilii）， Xuelianhua （Herba Saussureae Lanicepsis）， Yuejihua （Flos Rosae Chinensis）， Juhua （Flos Chrysanthemi） and other medicinal flowers are commonly used for IUA， as well as self-made compound formulas contained of these herbs such as No.1 IUA Formula which is used in the perioperative period of transcervical resection of adhesion， No.2 IUA Formula which is used to treat postoperative thin endometrium， and Zhuluan Formula （助卵方） for IUA patients who want to have children.

AIM:To investigate the effects of Panax notoginseng compound formula(PN)on endometrial fibro-sis by regulating inflammatory reaction and intestinal flora(IF)in a rat model of intrauterine adhesion(IUA).METHODS:The rat IUA model was established by following the mechanical injury method.A total of 50 rats were randomly divided in-to sham group,model group,low-dose(210 mg/kg)PN group,medium-dose(420 mg/kg)PN group and high-dose(840 mg/kg)PN group.After 8 weeks of intragastric administration,the uterus was collected to observe morphological changes with naked eye.The degree of uterine tissue damage and fibrosis was evaluated through hematoxylin-eosin(HE)and Mas-son staining.The collagen type Ⅰ(Col Ⅰ)was detected by immunohistochemistry.The interleukin-6(IL-6)and IL-10 pro-tein expression was detected by Western blot.The levels of IL-6,IL-1β,tumor necrosis factor-α(TNF-α)and vascular endothelial growth factor B(VEGFB)were detected by enzyme-linked immunosorbent assay(ELISA).The IF diversity and population structure were observed by 16S amplicon.RESULTS:Compared with the sham group,the uteruses of rats in the model group showed:reduced elasticity,accompanied by congestion and edema;decreased number of glands and blood vessels,and thinned endometrium(P<0.01);increased collagen fibers and Col Ⅰ protein expression(P<0.01);sig-nificantly increased IL-1β,IL-6,TNF-α and VEGFB levels in the uterine tissue(P<0.01);decreased IL-10 level(P<0.01);and reduced IF diversity(P<0.05).Compared with the model group,the drug intervention groups exhibited:re-covered elasticity of the uterus and relieved congestion and edema;increased number of endometrial glands and blood ves-sels(P<0.05);decreased collagen fibers and Col Ⅰ protein expression(P<0.01);reduced IL-1β,IL-6,and TNF-α lev-els to varying degrees in the uterine tissue(P<0.05);elevated IL-10 level(P<0.01);and improved IF diversity(P<0.05).CONCLUSION:The PN is able to significantly improve the endometrial tissue fibrosis in IUA rats.The under-lying mechanisms may be related to the inhibition of IL-6,IL-1β and TNF-α expression,up-regulation of IL-10,and im-provement of IF diversity.