Objective To determine the special diagnostic superiority of PCT test over CRP and WBC count test for neonatal sepsis .Methods 46 newborn inpatients diagnosed neonatal sepsis in the neonatal department of Dalian Central Hospital from Janu‐ary 2013 to January 2014 and contemporaneous 50 uninfected neonatal inpatients as control group were collected .The PCT and CRP concentrations and WBC count were simultaneously measured at admission .Results The WBC count had no statistical difference between the two groups ,but the PCT and CRP detection showed the statistical difference between the two groups (P<0 .01) ,the sensitivity of PCT test was 87% ,which was significantly higher than 63% of CRP test ,but the specificity of PCT test was 87% , which was slightly lower than 90% of CRP test .The Youden index of PCT test was obviously higher than that of CRP test and WBC count .Conclusion PCT is superior to CRP and WBC count in diagnosing neonatal sepsis .

Werner syndrome (WS) is a premature aging disorder that mainly affects tissues derived from mesoderm. We have recently developed a novel human WS model using WRN-deficient human mesenchymal stem cells (MSCs). This model recapitulates many phenotypic features of WS. Based on a screen of a number of chemicals, here we found that Vitamin C exerts most efficient rescue for many features in premature aging as shown in WRN-deficient MSCs, including cell growth arrest, increased reactive oxygen species levels, telomere attrition, excessive secretion of inflammatory factors, as well as disorganization of nuclear lamina and heterochromatin. Moreover, Vitamin C restores in vivo viability of MSCs in a mouse model. RNA sequencing analysis indicates that Vitamin C alters the expression of a series of genes involved in chromatin condensation, cell cycle regulation, DNA replication, and DNA damage repair pathways in WRN-deficient MSCs. Our results identify Vitamin C as a rejuvenating factor for WS MSCs, which holds the potential of being applied as a novel type of treatment of WS.
Animals ; Ascorbic Acid ; pharmacology ; Cell Cycle Checkpoints ; drug effects ; Cell Line ; Cellular Senescence ; drug effects ; DNA Damage ; DNA Repair ; drug effects ; DNA Replication ; drug effects ; Disease Models, Animal ; Heterochromatin ; metabolism ; pathology ; Humans ; Mesenchymal Stem Cells ; metabolism ; pathology ; Mice ; Nuclear Lamina ; metabolism ; pathology ; Reactive Oxygen Species ; metabolism ; Telomere Homeostasis ; drug effects ; Werner Syndrome ; drug therapy ; genetics ; metabolism