OBJECTIVE:To study the status and characteristics of the adverse drug reactions(ADR) presented in our hospital.METHODS:A total of 393 ADR cases collected during the period 2003～2007 in our hospital were analyzed statistically regarding the general status of the patients,route of administration,drug variety,the types of lesions and the clinical manifestations etc.RESULTS:Of the 393 ADR cases,the aged people(≥61 a) showed a highest incidence at 21.88%, 83.71%were induced by intravenous infusion,72.26%were induced by anti- infective drugs,lesions of skin and the appendants were the major type of ADR,accounting for 39.94%,and there were only 36 serious eases,accounting for 9.16%. CONCLUSION:ADR reporting and monitoring should be strengthened in hospital.Making full use of hospital ADR infor mation can facilitate rational drug use and improve clinical rational drug use level.

Objective To assess the clinical value of microguidewire looping technique in superselective catheterization of the acute angled artery. Methods Manipulating the microguide wire into a U shaped loop and simultaneus withdrawal of the microguidewire and microcatheter was performed when the tip of the microguidewire simultaneous got into the target artery and following by slowly and simultaneously withdrawing the microguidewire and microcatheter until the tip of the microguidewire entering into the target artery for a certain length and finally pushed the microcatheter into the target artery slowly. Results Eighteen out of 21 patients with acute angled target artery were successfully catheterized through this approach with technical success rate of 86%, without any complications. Conclusions Microguidewire looping technique is a feasible method for superselective catheterization of the acute angled artery when the routine approach failed.

Objectives:To investigate the experience in insertion and management of central venous catheter. Methods:132 cases received insertion of central venous catheter.The site of catheter tip was determined with the method of electrocardiograph.The insertion depth was calculated with method of Fujii.The catheter was managed with strictly sterile technique and its lumen was washed with 0.1 mol/L NaOH 2.0 ml. Results:All catheters were inserted smoothly and its tips lay in suitable sites.128 pieces of catheter were pulled out after finished infusion. Conclusions:A right method of insertion and management is in favor for the use of a central venous catheter.

OBJECTIVE: To establish a HPLC method for the determination of aripiprazole in human plasma by HPLC. METHODS: The plasma sample was determined by HPLC after subjected to liquid- liquid extraction .The separation was carried out on C18 column with clolumn temperature at 40℃. The mobile phase consisted of 0.03mol?L-1 ammonium acetate-acetonitrile(34:66) with a flow rate of 0.8mL?min-1 and detective wavelength of 257nm.The sensitivity was 0.01 AUFS. RESULTS:The linear range of aripiprazole was 5.0～600.0ng?mL-1(r=0.999 5) .The recovery rate was above 90% .CONCLUSION:The method is sensitive,accurate and rapid,and suitable for the determination of aripiprazole in human plasma.

The cases were randomly divided into 2 groups,each32 cases.The results were:for the Chinese druggroup.13 cases were markedly effective,15 Cases withrather good effect,2 cases improved.2 cases ineffec-tive.For the control group,the figures were 5,13,11,3 respectively.The total effective rate was similar forthe 2 groups,but the markedly effective rate for theformer is higher thanthe latter (P

Objective:To investigate whether VLA-5 and VLA-6 are involved in facilitating en-dothelium-oriented transmigration of hematopoietic stem/progenitor cells. Methods:Purified hu-man CD34+ cells were subject to ex vivo transmigration assay and blocking experiments throughtranswell filter inserts coated with human umbilical vein endothelial cells (HUVECs). Four-colorfluorescence-activated cell sorting (FACS) analysis was applied to detect the expression profilesof adhesion molecules and chemokine receptor CXCR-4 on CD34bright cells. Results:Stromal cell-derived factor (SDF)-1-induced transmigrations of both mobilized peripheral blood (mPB)-(56.6±20. 1)% and bone marrow (BM)- (15. 6±1. 8)% derived CD34+ cells were significantlyfacilitated through HUVECs-coated transwell filter insters compared with noncoated ones, whichwere efficiently blocked by preincubation of CD34+ cells with neutralizing antibodies to VLA-5,or VLA-6, or both of them; meanwhile the proportions of migrating CD34+ cells through bothHUVECs-coated and noncoated transwell filter inserts in BM were significantly lower than thosein mPB; the different percentages of migrating CD34+ cells between in mPB and BM were corre-lated with their variable expressions of VLA-5 and VLA-6, but not for VLA-4 or chemokine re-ceptor CXCR-4. Conclusion:Facilitating HS/PCs transmigrations through HUVECs are involvedin both VLA-5 and VLA-6.

BACKGROUND: Moxonidine is the second-generation high-selective central antihypertensive drug, while clonidine is the first-generation antihypertensive drug that is used in clinic with many side effects.OBJECTIVE: To compare the characteristics between moxonidine and clonidine in renal-hypertensive rats.DESIGN: Randomized controlled animal experiment.SETTING: Department of Pharmacology, Medical College, Nantong University.MATERIALS: The experiment was conducted in Medical College, Nantong University between September and December 2004. Totally 110 SD rats aged 60 days with the body mass of (180±30) g were used in the study.METHODS: Left renal artery stenosis in SD rats was established by inserting silver clip with the inner diameter of 0.2 mm or 0.25 mm, while the right renal artery was not received, so as to establish two-kidney one-clip(2K-1C) renal hypertensive models. ①Changes of blood pressure and heart rate in awake rats with renal hypertension were determined with arteria caudilis indirect manometric method, oral administration once or consecutively. The experiment of depressurization with once oral administration:The rats were randomly assigned into 5 groups with 10 rats in each group:1 mg/kg, 3 mg/kg, 10 mg/kg moxonidine hydrochloride groups, 1 mg/kg clonidine hydrochloride group were considered as positive control group,while saline group as negative control group. On the basis of the effect of moxonidine hydrochloride on blood pressure, blood pressure was measured at 1, 4, 24, 48, 72 hours after moxonidine administration, and compared with that before administration or the effect of saline. The experiment of depressurization with consecutively oral administration once a day. The grouping was the same to above-mentioned. Successive administration was for 7 days, once a day. The blood pressure and heart rate were determined at 1 hour before and after administration, and observed for 3 days after drug withdrawal. Recommended dose of moxonidine hydrochloride for human was about 0.4 mg/kg, while the oral dose for rats were around 0.04 mg/kg based on the animals' surface area. ②Changes of blood pressure and heart rate in anaesthesia rats with renal hypertension with a catheter on the carotid artery directly: 0.2 mg/kg drug liquor was given with gastric perfusion. The rats were randomly divided into 5 groups with 10 ones in each group: 0.13,0.4, 1.3 mg/kg moxonidine hydrochloride groups, 0.13 mg/kg clonidine hydrochloride group and saline control group. Mean arterial pressure was determined before and after administration at different time.MAIN OUTCOME MEASURES: Changes of blood pressure and heart rate in conscious and anesthetized renal-hypertensive rats before and after being administrated orally once or consecutively.RESULTS: All the rats were involved in the result analysis, without drop out during the trial. ①Moxonidine showed a dose-dependent effect on depressurization and descent of heart rate after once large dose oral administration in conscious renal-hypertensive rats. The 10-fold higher doses of moxonidine caused the same effects of clonidine. The decreasing of heart rate was little and short after consecutively small dose of oral administration of moxonidine, and which was similar to clonidine in percentage of depressurization. ②In anesthetized renal-hypertensive rats, moxonidine showed a dose-dependent effect on depressurization after once oral administration. There was no significant difference between moxonidine and clonidine in percentage of depressurization after 3 to 10-fold higher dose administration (P ＞ 0.05).CONCLUSION: The once higher dose oral administration of moxonidine has dose-dependent effect on depressurization for renal-hypertensive awake rats. Anesthesia. The effect of 10-fold dosage of moxonidine is equal to that of clonidine. The effect of 3-10-fold dosage of moxonidine is equal to that of clonidine in anesthesia renal-hypertensive rats. The small dose oral administration continuously of both moxonidine and clonidine with the same volume has the same depressurization effect in renal-hypertensive rats.

Objective:To investigate if chloride channel ClC-2 could be phosphorylated by mitogen activated protein kinase(MAPK) for further study of its regulation mechanism in proliferation and differentiation of cells.Methods:The coding sequence containing the cytosolic C-terminus of ClC-2 was amplified from pSPORT1/ClC-2 plasmid,including rabbit ClC-2 cDNA, by polymerase chain reaction(PCR),the fragment was cloned into pGEX-4T-1 plasmid for the construction of GST-tagged fusion protein expressing vector, pGEX-4T-1/ClC-2CT.After being identified by enzyme digestion and sequencing, the recombinant vector was transformed into a strain of E.coli BL21. The expression of GST-tagged fusion protein was induced with IPTG and purified with Gluthathion Sepharose 4B affinity chromatography. Then the phosphorylation of ClC-2 by MAPK was examined by using phosphorylation assays in vitro.Results:The construction of pGEX-4T-1/ClC-2CT recombinant vector was proved by enzyme digestion and sequencing. The purified fusion protein GST/ClC-2CT could be phosphorylated by MAPK, however the GST could not.Conclusion:Chloride channel ClC-2 can be phosphorylated by MAPK.

Objective To construct the inducible vector carrying green fluorescence protein(GFP). Methods We constructed an inducible vector pMD-GFP, including GFP cDNA, which allowed controlled expression of protein upon addition of 100 ?mol/L Zinc as an external inducer. The whole length of GFP cDNA were transfected into human hepatocellular cancer cells HCC-9204 by the method of lipofectin transfection. The expression of GFP was observed under fluorescence microscopy. Results The inducible vector carrying green fluorescence protein was successfully constructed. The observation under fluorescence microscopy showed that green fluorescence was spread in entire HCC-9204 cells transfected with GFP gene. Conclusion This new kind of the inducible vector could serve as a new tool and method for observing the growth and metastasis of neoplasm.

ObjectiveTo investigate the effect of p27 KIP1 transfection on cell cycle and apoptosis of hepatocellular carcinoma cells(HCC). MethodsWe used an inducible expression system pMD neo, which allowed controlled expression of protein upon addition of zinc as an external inducer. p27 KIP1 cDNA was transfected into human HCC 9204 cell line. Expression of p27 KIP1 was analyzed and cell growth was observed. ResultsExpression of p27 KIP1 in protein and mRNA increased significantly in HCC 9204 cell line transfected with p27 KIP1 . The cell growth reduced by 35%, p27 KIP1 over expression caused cell growth arrest at G 1 by 35% ( P =0 000). Apoptotic cell index significantly increased ( P =0 000).Conclusionp27 KIP1 may cause cell cycle arrest in G 1 phase and subsequently lead to apoptosis.