ObjectivesTo identified the strain 1012 from the National Center of Clinical Laboratory of China for microbe inter-laboratory quality assessment in 2010, and study the taxonomic status of strain 1012 and related species in the genus Actinomyces. Methods The bacterial traditional morphological characteristics, commercial API systems, and 16S rRNA gene sequence analysis were applied to identify the problematic culture of strain 1012. The phylogenetic tree based on the remote information of the prokaryotes systems was constructed to study the taxonomic status and evolutionary relationship of the genus Actinomyces and related species in the family Actinomycetaceae. Results Strain 1012 was determined as a kind of facuhative anaerobic,non-spot-forming,Gram-positive coryneformbacteria,which was identifiedto Actinomyces turicensis for the phenotypic biochemical characteristics of more than 60 items, The comparative study of 16S rRNA gene showed the strain 1012 with 99. 8％ similarities to Actinomyces turicensis, but only 90. 6％ to the type species of Actinomyces bovis in the genus Actinomyces. However, the comparative study of 16S rRNA gene showed the strain 1012 with only 90. 6％ homology to the type species of Actinomyces bovis in the genus Actinomyces. Further phylogenetic analysis showed that nine independent clusters were grouped in the family Actinomycetaceae, of which four clusters were separately represented the genera Varibaculum,Mobiluncus, Actinobaculum and Arcanobacterium, while other five clusters all were designated to the genus Actinomyces. The study showed strain 1012 was located in genus Ⅲ of Actinomyces, yet with a relatively long genetic distance to Actinomyces bovis. ConclusionThe genus Actinomyces may be reclassified as one genus Actinomyces sensu stricto and several new genera for the genotypic characteristics.

OBJECTIVETo prepare flexible proanthocyanidins nanoliposomes, and explore the in vitro release behavior of proanthocyanidins flexible nanoliposomes and general nanoliposomes.

METHODFlexible proanthoeyanidins nanoliposomes were prepared proanthocyanidins using a film dispersion method, characterized by transmission electron microscope, and the in vitro release action was studied in different dissolution mediums using dynamic dialyse method with the content of total phenol as index.

RESULTThe in vitro release of both proanthocyanidins flexible nanoliposomes and general nanoliposomes were in accordance with Weibull distribution.

CONCLUSIONProanthocyanidins flexible nanoliposomes without pressure had similar in vitro release behavior with general nanoliposomes.

Drug Delivery Systems ; methods ; Liposomes ; chemistry ; ultrastructure ; Nanospheres ; chemistry ; ultrastructure ; Particle Size ; Proanthocyanidins ; chemistry

Objective To investigate the changes of antimicrobial peptide LL-37 and C-reactive protein (CRP ) in the patients with Pseudomonas aeruginosa positive sputum culture and their mutual relation . Methods Fifty cases of Pseudomonas aeruginosa positive sputum culture and 27 cases undergoing physical ex-amination in the Guangdong Provincial Hospital of Chinese Medicine from September 2016 to May 2017 were selected as the research subjects .The level of antimicrobial peptide LL-37 was detected by double antibody sandwich ELISA and the CRP level was detected by immunoturbidimetry .Results The levels of antimicrobial peptide LL-37 and CRP in the Pseudomonas aeruginosa positive sputum culture group were significantly high-er than those in the healthy control group ,the difference was statistically significant (P< 0 .05) ,and they showed the positive correlation (r=0 .411 ,P<0 .05) .Conclusion In positive sputum culture of Pseudomonas aeruginosa ,antimicrobial peptide LL-37 and CRP levels are increased ,which has a certain clinical application value for the early diagnosis of infection .

Objective To explore the expression of Livin and Beclin1 in epithelial ovarian cancer and their relations with tumor invasion and metastasis. Methods A total of 65 patients with epithelial ovarian cancer who were admitted and treated in our hospital and Jilin University Affiliated Hospital, plus 15 blocks of normal ovarian tissue samples were selected. Excised ovarian tissues were tested via immunohistochemistry to test the protein expression of Livin and Beclin1, and their relations with tissue differentiation of ovarian cancer, lymphatic metastasis and clinical staging were analyzed. Results Positive rate of expression of Livin, rare expression in normal ovarian tissues (6.7%), and the highest expression rate in tissues of ovarian cancer (68.6%);Positive rate of expression of Beclin1,the highest expression rate in normal ovarian tissues (93.3%) and the lowest in tissues of ovarian cancer (37.1%). Tissue differentiation of ovarian cancer, lymphatic metastasis and clinical staging were interrelated with the expression degree of Livin and Beclin1 (P<0.05). Conclusion Expression of Livin and Beclin1 in epithelial ovarian cancer and tumor invasion and metastasis plays an important role and may become a new target in the treatment of ovarian cancer.

Objective To analyze the senile lying-in woman whose psychological characteristics and the effect of psychological nursing intervention. Methods Senile women prenatal and childbirth in our hospital were selected as the research objects, who were respectively given routine nursing and corresponding psychological nursing intervention,the differences of the second labor,delivery methods,perinatal negative emotion score of senile lying-in women of two groups,and so on were compared. Results The second labor time (34.82±14.31)min of senile lying-in women of observation group, oxytocin utilization rate (6.25%), prenatal negative emotions scores of HAMD (15.14±1.35),HAMA(13.34±1.11),postpartum negative emotions scores HAMD(12.53±1.64),HAMA(11.41±1.09) were all obviously lower than those of control group.The rate of vaginal delivery (79.69%) was significantly higher than that of control group(P<0.05). Conclusion Pertinent psychological nursing can effectively shorten the labor time of women and raise the rate of vaginal delivery,effectively relieve the perinatal negative emotion.

Pyogenic liver abscess (PLA) accompanied by occult malignant tumors is a rare kind of life-threatening disease. Studies have shown that it can predict the occurrence of cancer, especially hepatobiliary and colorectal cancer. The risk of combined occult primary liver cancer, cholangiocarcinoma, and gastrointestinal cancer is high in PLA patients. Malignant tumor-related PLA lacks specific symptoms and signs. The iodine concentration ratio between the energy spectrum CT lesions and normal liver tissue is of certain value in the differentiation of liver cancer and liver abscess. Computed tomography colonography has a dual role. It can screen patients with PLA for occult colorectal cancer and determine the treatment response of abscess lesions. Klebsiella pneumoniae and Escherichia coli is the main microorganism of PLA related to colorectal cancer, hepatocellular carcinoma, and intrahepatic cholangiocarcinoma. PLA treatment related to hepatobiliary malignant tumor has high complications and mortality, and poor prognosis. Most occult colorectal cancers are in the early stage, and their early detection and prognosis are better than those of PLA patients combined with hepatobiliary malignancies.

Objective To explore the application of the infusion management system in ICU and its effect on the major nursing risk factors .Methods One hundred and five patients with top ten diseases in ICU from March 2013 to December 2013 were chosen and were randomly divided into the control group ( n=50 ) and the observation group ( n =55 ) .The control group received the management of liquid therapy through the monomer infusion pump , and the observation group received the management of liquid therapy through B .Braun infusion therapy system .The average time of liquid therapy , the rate of unplanned extubation , the error rate of vasoactive drugs and the nurses ’ satisfaction of two groups were observed .Results The average time of liquid therapy, the rate of unplanned extubation , the error rate of vasoactive drugs and the nurses ’ satisfaction were respectively (65.3 ±21.4)h, 7.3%, 7.3%, 92.7%in the observation group, and were better than (99.4 ± 27.8)h, 16.0%, 14.0%, 56.0% in the control group, and the differences were statistically significant (t/χ2 =2.63, 7.25, 5.38, 8.68, respectively;P<0.05).Conclusions Application of B.Braun infusion therapy system in ICU can reduce the workload of nurses , and decrease the rate of unplanned extubation and the error rate of vasoactive drugs in patients , and increase the nurses ’ satisfaction.

Objective To explore the relationships of β2-microglobulin(β2-MG),retinol bind-ing protein(RBP)and aquaporin-2(AQP2)in serum and urine with the severity of hydronephrosis induced by ureteropelvic junction obstruction(UPJO)in children.Methods Sixty children with hy-dronephrosis who underwent surgery for UPJO at the Department of Urology of the Sixth People's Hos-pital of Nanning were selected as hydronephrosis group.According to the severity of hydronephrosis,they were divided into mild group(n=13),moderate group(n=22),and severe group(n=25).Meanwhile,60 healthy children in the same period were selected as the control group.The serum and urine levels of β2-MG,RBP and AQP2 were compared between control group and children with hydronephrosis of hydronephrosis group.The correlation between the levels of β2-MG,RBP,AQP2 and ultrasound parameters[anteroposterior diameter of renal pelvis(APD),parenchymal thickness(PT)]was analyzed.The receiver operating characteristic(ROC)curves were used to analyze the diagnostic efficacy of β2-MG,RBP and AQP2 in serum and urine for moderate to severe hydrone-phrosis.Results The levels of β2-MG and RBP in serum and urine of the hydronephrosis group were higher than those in the control group,and the levels of AQP2 in serum and urine were lower than those in the control group(P＜0.05).The levels of β2-MG inserum and urine,RBP in urine,and APD from low to high in different groups were as follows:mild group,moderate group to severe group.Urine AQP2 level and PT from high to low in different groups was as follows:mild group,moderate group to severe group(P＜0.05).Serum RBP level in the mild group was lower than those in the moderate group and the severe group(P＜0.05).Urinary AQP2level was negatively correlated with APD and positively correlated with PT(P＜0.05).ROC curve analysis results showed that serum β2-MG,urine β2-MG,urine RBP,urine AQP2 detection had certain diagnostic value in moderate and severe hydronephrosis,and the area under the curve(AUC)was 0.652,0.688,0.859,0.680,respectively.The AUC of urinary RBP in the diagnosis of moderate and severe hydronephrosis was significantly higher than that of other indexes(P＜0.05).Conclusion β2-MG in serum and urine,urine RBP and urine AQP2 are related to the severity of hydronephrosis induced by UPJO in children.Urine RBP level has better diagnostic efficacy for moderate to severe hydrone-phrosis than other indicators.

Objective To explore the relationships of β2-microglobulin(β2-MG),retinol bind-ing protein(RBP)and aquaporin-2(AQP2)in serum and urine with the severity of hydronephrosis induced by ureteropelvic junction obstruction(UPJO)in children.Methods Sixty children with hy-dronephrosis who underwent surgery for UPJO at the Department of Urology of the Sixth People's Hos-pital of Nanning were selected as hydronephrosis group.According to the severity of hydronephrosis,they were divided into mild group(n=13),moderate group(n=22),and severe group(n=25).Meanwhile,60 healthy children in the same period were selected as the control group.The serum and urine levels of β2-MG,RBP and AQP2 were compared between control group and children with hydronephrosis of hydronephrosis group.The correlation between the levels of β2-MG,RBP,AQP2 and ultrasound parameters[anteroposterior diameter of renal pelvis(APD),parenchymal thickness(PT)]was analyzed.The receiver operating characteristic(ROC)curves were used to analyze the diagnostic efficacy of β2-MG,RBP and AQP2 in serum and urine for moderate to severe hydrone-phrosis.Results The levels of β2-MG and RBP in serum and urine of the hydronephrosis group were higher than those in the control group,and the levels of AQP2 in serum and urine were lower than those in the control group(P＜0.05).The levels of β2-MG inserum and urine,RBP in urine,and APD from low to high in different groups were as follows:mild group,moderate group to severe group.Urine AQP2 level and PT from high to low in different groups was as follows:mild group,moderate group to severe group(P＜0.05).Serum RBP level in the mild group was lower than those in the moderate group and the severe group(P＜0.05).Urinary AQP2level was negatively correlated with APD and positively correlated with PT(P＜0.05).ROC curve analysis results showed that serum β2-MG,urine β2-MG,urine RBP,urine AQP2 detection had certain diagnostic value in moderate and severe hydronephrosis,and the area under the curve(AUC)was 0.652,0.688,0.859,0.680,respectively.The AUC of urinary RBP in the diagnosis of moderate and severe hydronephrosis was significantly higher than that of other indexes(P＜0.05).Conclusion β2-MG in serum and urine,urine RBP and urine AQP2 are related to the severity of hydronephrosis induced by UPJO in children.Urine RBP level has better diagnostic efficacy for moderate to severe hydrone-phrosis than other indicators.