1.Radiobiological effect of 131I radiolabeled recombinant human epidermal growth factor on nude mice with human breast cancer.
Yunchun LI ; Weiyun XU ; Tianzhi TAN ; Sheng HE
Journal of Biomedical Engineering 2005;22(4):749-753
The radiobiological effect of 131I radiolabeled recombinant human epidermal growth factor (131I-rhEGF) on nude mice with human breast cancer was assessed in this study. The tissue mainly uptaking 131I-rhEGF was found by tissue distribution assay in mice. The radiation breakdown of the tissue greatly collecting 131I-rhEGF was examined by biochemical test and biopsy in nude mice with human breast cancer. The tissue distribution assay of 131I-rhEGF in mice showed that 131I-rhEGF greatly accumulated in kidney, liver, spleen and blood. The biochemical test and biopsy revealed that 131I -rhEGF injected twice (dosing once is analogous to 14.58 GBq in a person with 50 kg, once every 14 days) had an effective killing effect on tumor but had no effect of radiation breakdown on kidney, liver,spleen and blood-cell forming tissue in mice with human breast cancer. Therefore, 131I-rhEGF is a drug unharmful to normal tissues in the course of the receptor-mediated target radiotherapy for breast cancer.
Animals
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Breast Neoplasms
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metabolism
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pathology
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radiotherapy
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Cell Line, Tumor
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Drug Delivery Systems
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Epidermal Growth Factor
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biosynthesis
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genetics
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pharmacokinetics
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therapeutic use
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Humans
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Iodine Radioisotopes
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Mice
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Mice, Nude
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Neoplasm Transplantation
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Radiopharmaceuticals
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Random Allocation
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Recombinant Proteins
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biosynthesis
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genetics
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pharmacokinetics
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therapeutic use
2.Experimental research into the inhibitory effect of 131I-recombinant human epidermal growth factor on the growth of breast cancer in vivo.
Yunchun LI ; Weiyun XU ; Tianzhi TAN ; Sheng HE
Journal of Biomedical Engineering 2004;21(3):444-448
This experiment was designed to study the effect of 131I-recombinant human epidermal growth factor (131I-rhEGF) on the growth of tumor in nude mice loaded with human breast cancer. Bioactivity of 131I-rhEGF and uptake of 131I-rhEGF in breast cancer tissue were verified using biodistribution experiment of 131I-rhEGF in the nude mice loaded with human breast cancer. The effect of 131I-rhEGF on the growth of tumor was assessed via the growth experiment of tumor in the nude mice loaded with human breast cancer. The ultrastructural change of the tumor cell treated with 131I-rhEGF was observed under transmission electron microscope, and the pathological change of the tumor tissue treated with 131I-rhEGF was detected by biopsy. The results showed that the tumor tissue of nude mice bearing human breast cancer obviously takes in 131I-rhEGF; that intravenous administration and intratumoral administration of 131I-rhEGF both obviously inhibit the growth of tumor, the inhibition rates (82.00% and 80.70%) being remarkably higher than that of 131I (7.49%) and that of 131I-HSA (6.91%) (P<0.05); and that intravenous and intratumoral administration of 131I-rhEGF both obviously damage and kill tumor cells. Therefore, 131I-rhEGF can inhibit the growth of human breast cancer cell in nude mice; it is a potential receptor-mediated radioactivity targeting drug for treating breast cancer.
Animals
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Breast Neoplasms
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pathology
;
ultrastructure
;
Drug Delivery Systems
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Epidermal Growth Factor
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biosynthesis
;
genetics
;
pharmacology
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Female
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Humans
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Iodine Radioisotopes
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administration & dosage
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pharmacology
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Mice
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Mice, Inbred BALB C
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Mice, Nude
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Neoplasm Transplantation
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Receptor, Epidermal Growth Factor
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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pharmacology
3.Application of zebrafish models in research of diabetes
Xue WANG ; Liwen HAN ; Qiuxia HE ; Jian HAN ; Rongchun WANG ; Weiyun CHEN ; Ximin WANG ; Hairong HOU ; Kechun LIU
Chinese Journal of Comparative Medicine 2017;27(8):1-5
As a model organism, zebrafish have many advantages over other animal models and is suitable for studies on establishment of human disease model and mechanism.In zebrafish, there are two phases of endocrine formation during early development, which are directed by concomitant activity of many signaling pathways.Zebrafish pancreas possess similar cell structure with that of other animals, which can express various endocrine hormones including insulin.The main organs required for metabolic control, such as the pancreas, islet, and insulin sensitive tissue (muscle, liver) are conserved in zebrafish, and the mechanisms of glucose regulation in zebrafish is similar to that seen in mammalian models.These render it an excellent model to study glucose metabolism.Hyperglycemia in zebrafish model can be induced by administration of the diabetogenic drug, streptozotocin (STZ), alternatively immersion of the fish in glucose solution and water, or disturbing of signaling pathways associated with glucose metabolism.Glucose levels in adult zebrafish blood or embryo tissue and phenotype of retinal cell layers or retinal vasculature are the commonly used measurement organs in zebrafish diabetic models.
4.Scoliosis and associated factors among middle school students in Guangzhou City
SUN Yi, LIU Weijia, XIONG Lihua, LI Meng, CHEN Siyu, HE Weiyun, WU Deping, LIN Rong
Chinese Journal of School Health 2021;42(12):1867-1870
Objective:
To investigate the general situation of scoliosis and influencing factors among middle school students in Guangzhou, so as to provide evidence for behavioral intervention measures.
Methods:
By stratified cluster random sampling method, 2 121 students from 8 middle schools of Guangzhou were selected to conduct questionnaire survey and physical examination.
Results:
The detection rate of scoliosis among middle school students in Guangzhou was 8.20%, girls (9.50%)>boys (7.00%), urban area (10.45%)>suburb area (4.77%), senior high school (10.08%)>junior school (6.39%) ( P <0.05). Among the most of the 174 positive students were found to have large thoracic curve, right lateral bending, being girls with medium scoliosis. Multivariate Logistic regression analysis showed that students in urban area ( OR=2.56, 95%CI =1.71-3.82), with mobile electronic devices usage time≥3 h/d( OR=1.59, 95%CI =1.12-2.27), prolonged near vision work ≥1 h ( OR=1.40, 95%CI =1.00-1.95), outdoor activity time<2 h/d( OR=1.82, 95%CI =1.24-2.67) had a higher detection rate of scoliosis ( P <0.05).
Conclusion
The detection rate of scoliosis among middle school students in Guangzhou is much higher, which might be related to district, mobile electronic devices usage, prolonged near vision work and insufficient outdoor activity. Health education regarding spinal knowledge should be strengthened.
5.Clinical features and outcome of posttraumatic endophthalmitis in children
Xiaozhen HE ; Fengjie LI ; Peiyan SHI ; Jijun SUN ; Weiyun SHI ; Ting WANG
Chinese Journal of Experimental Ophthalmology 2019;37(7):553-558
Objective To investigate the clinical feature and treatment outcome of paediatric posttraumatic endophthalmitis.Methods A retrospective case study was performed.Twenty-six paediatric posttraumatic endophthalmitis children with 26 eyes were enrolled in Shandong Eye Hospital from April 2014 to April 2017.The clinical features,causes of trauma,time and mode of treatment,complications and visual acuity were analyzed.The subjects were graded according to the prognostic status,and the prognosis of the children was compared among different treatment time,vitrectomy time,operation frequency and antibiotic application.Results Of 26 cases,including 20 males and 6 females,aged from 1 year to 13 years,the average age was (6.56±3.25) years.The subjects were divided into infant group (2 cases,7.69%),preschool group (10 cases,38.46%) and school age group (14 cases,53.85%).The main cause of injury was wire,accounting for 34.62% (9/26).Syringe needles accounted for 15.38% (4/26).Fireworks accounted for 11.54% (3/26).All of the 26 cases received vitrectomy,24 cases combined with lens extraction (92.31%).Among the 26 cases,8 cases had no primary visual acuity record;in the remaining 18 cases,the visual acuity at last follow-up was significantly improved when compared with the preoperative primary visual acuity (x2 =7.385,P =0.033).The positive rate of pathogenic bacteria culture in aqueous humor and vitreous humor was 23.08% (6/26),gram-positive cocci was the main pathogenic bacteria of posttraumatic endophthalmitis,which account for 83.3% (5/6).The prognostic grade was grade 1 in 15 cases (57.7%),grade 2 in 7 cases (26.9%) and grade 3 in 4 cases (15.4%).There were significant differences in the number of prognostic grading cases among children with different hospitalizing time or vitrectomy time (all at P<0.05).The hospitalizing time,time of vitrectomy,number of operations and systemic application of antibiotics were positively correlated with the prognosis grade (rs =0.580,0.414,0.428,0.535;all at P < 0.05).Conclusions Children with posttraumatic endophthalmitis should undergo vitrectomy as early as possible,which can receive better prognosis.
6.Factors associated with overweight or obesity in community patients with schizophrenia in Shanghai
Yanli LIU ; Weibo ZHANG ; Siyuan HE ; Weiyun XU ; Qing ZHOU ; Yihua JIANG ; Yanping ZHANG ; Jun CAI
Shanghai Journal of Preventive Medicine 2023;35(5):426-432
ObjectiveTo investigate the prevalence of overweight or obesity in community patients with schizophrenia in Shanghai and to explore the related factors. MethodsStratified cluster sampling method was used and the general condition, physical examination and laboratory examination data of patients with schizophrenia who voluntarily participated in 2020 free health examination of National Basic Public Health Service were analyzed. ResultsA total of 3 200 patients were included into the study ,and the prevalence of overweight and obesity was 36.75% and 17.19%, respectively. Multivariate logistic regression analysis indicated that age between 40 and 60 (OR=1.333, 95%CI: 1.030‒1.724), intake of first-generation antipsychotics (OR=1.413, 95%CI: 1.112‒1.796), intake of second-generation antipsychotics (OR=1.573, 95%CI: 1.288‒1.921), high-normal blood pressure (OR=1.549, 95%CI: 1.245‒1.927), high-abnormal blood pressure (OR=2.824, 95%CI: 2.204‒3.619), elevated ALT (OR=1.874, 95%CI: 1.386‒2.535), elevated FBG (OR=1.270, 95%CI: 1.066‒1.513), and elevated TG (OR=1.652, 95%CI: 1.335‒2.044) were the related factors that associated overweight or obesity in patients with schizophrenia. ConclusionOverweight and obesity are highly prevalent among community patients with schizophrenia in Shanghai. Age between 40 and 60, taking first-generation and second-generation antipsychotics, blood pressure higher than 120/80 mmHg, elevated ALT, elevated FBG, and elevated TG are associated with overweight or obesity in patients with schizophrenia. To provide personalized health guidance, medical staff in primary health care institutions should pay more attention to high-risk groups of overweight and obesity in schizophrenia patients at annual physical examination.
7. Applicability and feasibility of "Lingnanzhun" -an "Internet Plus-based HIV Self-testing Tool" targeting MSM in Guangzhou
Yanshan CAI ; Yuzhou GU ; Fei ZHONG ; Lirui FAN ; Yuteng ZHAO ; Yefei LUO ; Yongheng LU ; Weiyun HE ; Weibin CHENG ; Huifang XU
Chinese Journal of Epidemiology 2019;40(10):1212-1216
Objective:
This article was to evaluate the applicability and feasibility of "Lingnanzhun" -an "Internet Plus-based HIV Self-testing Tool" targeting MSM in Guangzhou. Hopefully, the results could be used to improve the existing HIV testing services and to support the implementation and scale-up of HIV self-testing programs.
Methods:
Data were collected from a survey on HIV testing preferences among the Internet-using MSM in April to June, 2015. Univariate and multivariate logistic regression analysis were applied to identify factors associated with the use of HIV self-testing service provided by Lingnanzhun. Information related to the users of Lingnanzhun during September 2014 and December 2018 was also collected.
Results:
769 MSM were recruited as participants. Of them, age distribution was 16-77(28.6±6.8) years old, 88.3
8. Characteristic analysis among MSM-users of the "Online HIV Acquisition Risk Assessment System" in Guangzhou
Yefei LUO ; Yuzhou GU ; Fei ZHONG ; Huifang XU ; Yanshan CAI ; Lirui FAN ; Yuteng ZHAO ; Zhigang HAN ; Weiyun HE ; Gang MENG ; Xiaofei JIA ; Weibin CHENG
Chinese Journal of Epidemiology 2019;40(10):1217-1221
Objective:
To analyze the characteristics of levels related to the risk through self-evaluation system, among MSM users in Guangzhou, between 2015 and 2017.
Methods:
Between 2015 and 2017, data was collected from the users of a self-evaluation system network related to HIV infection, based on the previous 'HIV health risk appraisal model’. Information on risk factors was collected to calculate the scores and levels of risks and to estimate the incidence of HIV. Taking the reference of
9. Effectiveness of the "Internet Plus-based AIDS Comprehensive Prevention Service System" among MSM in Guangzhou
Yuzhou GU ; Weibin CHENG ; NgaiSie WONG ; Fei ZHONG ; Yanshan CAI ; Yefei LUO ; Zhigang HAN ; Weiyun HE ; Qi LIU ; Jiewei LIU ; Huifang XU
Chinese Journal of Epidemiology 2019;40(10):1222-1226
Objective:
To evaluate the effectiveness of the "Internet Plus-based AIDS Comprehensive Prevention Service System" among men who have sex with men in Guangzhou.
Methods:
Data through case-reporting and follow-up programs on MSM HIV/AIDS in Guangzhou was collected from the China Information System for Disease Control and Prevention, which including those from the referral and follow-up treatment compliance programs in 2008-2014 (pre-treatment) and 2017-2018 (post-treatment). According to the types of care services, three groups were set as: with "Internet Plus" service, with 'HIV counseling/testing service’ or with 'routine medical service’. General Estimating Equation (GEE) was used to analyze the follow up situation of HIV/AIDS cases, annually. Cox proportional hazard regression model was used to analyze the proportions of treatment referral, within the 30 days of diagnosis.
Results:
Before the implementation of immediate treatment after HIV diagnosis, 90.6
10. Status quo and characteristic analysis among MSM-users of the "Internet Plus-based AIDS Comprehensive Prevention Service System" in Guangzhou
Weibin CHENG ; Shunming LI ; Yuzhou GU ; Fei ZHONG ; Wenting HUANG ; Yefei LUO ; Yanshan CAI ; Weiyun HE ; Lirui FAN ; Yuteng ZHAO ; Huifang XU ; Weiming TANG
Chinese Journal of Epidemiology 2019;40(10):1206-1211
Objective:
To analyze the characteristics of the "Interner Plus-based AIDS Comprehensive Prevention Service System" among MSM who frequently using the Internet in Guangzhou.
Methods:
An online survey was conducted among MSM who were recruited through gay-website portals between August and September, 2018 in Guangzhou, to collect information regarding the use of and attitudes on the "Interner Plus-based AIDS Comprehensive Prevention Service System" . Logistic regression was used to explore the association between the use of Internet intervention tools and related behavioral characteristics. Information on the awareness of AIDS, HIV testing, and condomless anal sex behavior were compared between the core or non-core services users.
Results:
A total of 777 Internet-based MSM were recruited as participants including 638 men (82.1