Objective:To detect mutations of exons 5 and exons 8 of PTEN gene in oral squamous cell carcinoma(OSCC),and to explore the relationship between gene mutations and development of OSCC.Methods: Mutations of exons 5 and exons 8 of PTEN gene were detected by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP).Results: The whole sequences of exons 5 and exons 8 of PTEN gene of all cases of OSCC were expanded.There was no mutation in exons 5 and exons 8 of PTEN gene of all cases of OSCC.Conclusion: There is no mutation in exon 5 and exon 8 of PTEN gene in OSCC.This might show that there is no correlation between mutations in exon 5 and exon 8 of PTEN gene and the development of OSCC.

OBJECTIVETo study the effect of light intensity on photosynthetic characteristics and seedling growth of Prunella vulgaris under different light intensity.

METHODThe seedling of P. vulgaris was shaded with shading net, and relative parameters were measured. The data was statistically processed.

RESULTWith the reducing light intensity, the young plant stems became slender, the leaf long increased. The plant height and net growth under 42% full light condition were significantly higher than those under the other light intensity, chlorophy II and carotenoid contents increased, as well as Chl a/b decreased under low light. Photosynthetic indicators of P(n), T(r) decreased under low light, but CO2 concentration increased, and G(s) content decreases and then increased under 10% full light intensity.

CONCLUSIONP. vulgaris manifested the different plasticity and adaptability under different light intensities. The 42% light intensity was the best and the proper shading would promote the growth of seedlings.

Adaptation, Physiological ; Chlorophyll ; metabolism ; Light ; Photosynthesis ; radiation effects ; Plant Leaves ; growth & development ; radiation effects ; Plant Stems ; growth & development ; radiation effects ; Prunella ; growth & development ; radiation effects ; Seedlings ; growth & development ; radiation effects

OBJECTIVETo study the effect of water stress on physiological and the growth characters of Prunella vulgaris at the vegetative stage.

METHODThe plants were treated timely and quantitatively by weighting method, and the relevant index were measured under different water stress conditions.

RESULTWith the long time of water stress, the plants of SY2 and SY3 showed the quickest growth rate and the best of growth vigor. The protective enzyme and osmoregulation substance presented the trend of increase firstly and then decrease. Under the same time of water stress, with the increase of extent in treatment, the protective enzyme and osmoregulation substance presented the trend of increase firstly and then decrease, and SY2 and SY3 were the lowest.

CONCLUSIONThe protective enzyme and osmoregulation substance of P. vulgaris have a whole effect. The suitable water range of growth in P. vulgaris is 65%-80%.

Prunella ; growth & development ; physiology ; Stress, Physiological ; Water ; metabolism