Objective To characterize the relationship between diabetic peripheral neuropathy(DPN) and serum anti gangliosides antibody(GS). Methods Elderly patients were divided into three groups: patients with type 2 diabetes mellitus(DM) showing no DPN,DM group, 25; DPN group, 32; healthy control group, 30. The nerve conduction velocity was measured using Nicolet Viking type IV electromyography(USA). The anti GSIgM Ab and anti GSIgG Ab were examined by solid enzyme immunoassay, and interleukin 1?(IL 1?), IL 2,tumor necrosis factor ?(TNF ?), HbA 1C and NO were also measured. Results The positive rate of anti GSIgM Ab and anti GSIgG Ab in DPN group being 65.6% and 34.4% respectively, was obviously higher than those in N group and DM group(P

Objective To observe the clinical efficacy of amitriptyline with manipulative therapy for episode tension-type headache (ETTH). Methods 62 patients were randomized into 2 groups: amitriptyline group, amitriptyline with manipulative therapy group. Numbers of days with headache(NDH) and days with medication(NDM), intensity of the headache(IH) between the two weeks before randomisation and 1～2, 3～4 weeks after randomization were recorded by participants in headache diaries. Results In the first 2 weeks after treatment, the NDH in amitriptyline group and amitriptyline with manipulative therapy group were (6.00±2.21) d and (4.19±1.70) d respectively (P<0.05), while in the second 2 weeks, they were (4.00±1.86) d and (2.16±1.21) d respectively (P<0.05). The NDM in amitriptyline group and amitriptyline with manipulative therapy group were (1.77±1.23) d and (1.32±1.22) d respectively in the first 2 weeks after treatment (P<0.05), but there was no significantly difference between the two groups in the second 2 weeks. The IH (with Visual Analog Scale) were(3.32±0.94) and (2.39±1.33)respectively (P<0.05) in the second 2 weeks. Conclusion Amitriptyline with manipulative therapy is better than amitriptyline alone in reducing the symptoms of episode tension type headache.

Objective Inquire into the relationship between diabetic peripheral neuropathy(DPN)pathogenic factor and Antigangliosides antibody(Anti-GS-Ab)in type 2 diabetes mellitus. Methods It was examined by enzyme-linked immunosorbent assays(ELISA)to the levels of serum Anti-gangliosides(Anti-GS)in 2 DM and DPN as well as healthy.Results The positive rate of Anti-GS-IgM,IgG in DPN group were 46.7% and 20.0%.repectivily it was obviously higher than normal group and 2 DM group.Conclusion The relationship between the DPN and Anti-GS-Ab is a close.It show that Anti-GS-Ab play an important role in DPN pathological process.

OBJECTIVE:To prepare the compound chlorhexidine acetate ear drops for treating anaerobic and aerobic infections of antrum auris METHODS:The compound chlorhexidine acetate ear drops was prepared with mixed solvent of glycerin,alcohol and distilled water The contents of two main ingredients were determined by dual-wavelength isobestic point spectrophotometry and the stability of preparation was examined RESULTS:The average recovery of metronidazole was 99 34%(RSD=0 57%,n=6) and that of chlorhexidine acetate was 101 17%(RSD=0 88%,n=6) CONCLUSION:The new preparation is rational in formula,simple in quality control and good in stability and has good prospects in development

Stent-assisted angioplasty of intracranial and extracranial arteries is a complex pathophysiologic process.Therefore,understanding of related factors is significant for guiding clinical treatment and reducing restenosis rate.Intravascular stent as a foreign body leads to platelet activation and aggregation,cytokine secretion,resulting in thrombosis and leukocyte aggregation at vessel injury site to produce cytokine and inflammatory reaction.In addition,the smooth muscle cells migrate to injury site and proliferate,leading to intimal hyperplasia,vessel wall reconstruction and intra-stent restenosis.Moreover,the restenosis following stenting is related to stent type and medicine supportive treatment.Drug-eluted stent with anti-inflammation and vascular smooth muscle cell proliferation can reduce body inflammation intensity and duration,and postoperative medicine supportive treatment can reduce incidence of intravascular restenosis.

BACKGROUND: The role of inflammatory reaction and inflammatory factors and the mechanism of pathophysiology of restenosisfollowing the stent implantation remains controversial. OBJECTIVE: To observe the changes of platelet activation markers and correlated factors following carotid artery stenting in rabbits. DESIGN, TIME AND SETTING: Observation experiment was performed at the Institute of Neurobiology of Henan University fromMarch to September 2008. MATERIALS: A total of 26 rabbits, male or female, weighing 2.1-2.9 kg, were included. The alloy stent was made of 90% platinumand 10% iridium with wire diameter of 0.13 mm and monofilament winding spiral structure. The alloy stent was submerged 100 g/Lgelatin solution, and made into protein-coated stent after dried. METHODS: Animals were anesthetized by sodium pentobarbital (30 mg/kg), and the right carotid artery was exposed. The stent was inserted in the proximal end after administration of aspirin and clopidogrel dual antiplatelet therapy. Penicillin was intravenously injected, 800 000 IU per day. MAIN OUTCOME MEASURES: The platelet-activating factor (PAC-1 and CD62P) of peripheral blood, platelet-monocyte aggregates and interleukin (IL-6) immediately before and 0.5 hour, 6 days, 1 month and 6 months after implantation were determined.RESULTS: Twenty-six rabbits were included in the final analysis. The level of PAC-1, CD62P and platelet-monocyte aggregates at 0.5 hour, 6 days and 1 month after inserting were significantly elevated compared with the levels immediately before inserting (P

Objective According to the special changes of ECG in hyperkalemia animals, explore a method to reproduce a rabbits model for hyperkalemia. Methods Three programs were applied by intravenous drip of 3%KCl to reproduce the hyperkalemia model. In the first program,once the wide and deformed QRS complex occurred, the potassium drip should be stopped immediately. In the second program,the wide and deformed QRS complex was kept for 30 min by adjusting the speed of potassium infusion. In the third program, once low and flat P wave and peaked T wave appeared, immediately adjusted the speed of potassium infusion in order to keep for 30 min. And HR, BP, urine output and serum potassium were monitored simultaneously. Hyperkalemia is defined as serum potassium≥5.1 mol/L. The successful hyperkalemia model should keep serum potassium≥5.1 mol/L after ceasing potassium given 30 min. Results The second and third programs could reproduce a hyperkalemia model successfully. The serum potassium returned to normal within 30 min after stopping potassium given in the first program. Conclusion The method which keep low and flat P wave and peaked T wave for 30 min and keep the wide and deformed QRS complex 30 min could reproduce the hyperkalemia model successfully.

Objective To investigate antithrombotic and antiplatelet effects of Dragon's Blood,?-cyclodextrin inclusion of Dragon's Blood.Methods The methods of injection of adenosin diphosphate(ADP) into mouse tail vein,electrically stimulated arterial thrombosis in rats were used to evaluate the anti-thrombotic effects of Dragon's Blood and?-cyclodextrin inclusion of Dragon's Blood,respectively.Platelet aggregation(PRP or washed platelets) was tested according to Born's method.Results ?CYDB significantly protected against thrombosis caused by ADP on mice,and electrical stimulation on rats.In vitro?-cyclodextrin inclusion of Dragon's Blood inhibited AA,ADP,PAF-induced platelet aggregation.Conclusions The results suggest that compared with Dragon's Blood,?-cyclodextrin inclusion of Dragon's Blood has more potent anti-thrombotic effects on the thrombotic models,its mechanisms may be closely related to the inhibition of anti-platelet aggregation.?-cyclodextrin inclusion of Dragon's Blood can improve the bioavailability of Dragon's Blood.

ObjectivesTo retrospectively analyze the clinicopathological features of neuroblastoma (NB) and investigate the signiifcance of abnormality ofN-myc and anaplastic lymphoma kinase (ALK) gene copy number change as well asALKmu-tations in NB.Methods Eighty-three NB patients were collected and classiifed into different subgroups according to the clinical stage and histology. Fluorescence in situ hybridization (FISH) was performed to detect the abnormalities ofN-mycandALK genes. The extracted DNA was ampliifed by PCR and sequenced to investigate the point mutations of theALK gene. Follow-up data were collected and survival analysis was performed.ResultsFISH detection showed that the aberration ofN-mycgene copy number presented as gain and ampliifcation. The aberration ofALK gene presented as point mutation and gain. It was shown that 17 cases had the abnormality of bothN-myc andALK gene. Survival analysis showed that the prognostic factors included the clinical stage, age and abnormality ofN-myc genes.ConclusionDetection ofN-myc andALK abnormality in NB would be helpful for evaluating the prognosis and providing theoretical basis forALK target therapy.

AIM: To investigate the effect of non-activated or activated polymorphonuclear leukocytes(PMN) on washed platelet aggregation. METHODS: Born's method was used to determine platelet aggregation.RESULTS: non-activated PMN (5?10 9 cells/L) significantly suppressed washed platelet aggregation induced by ADP or arachidonic acid. Aspirin enhanced this inhibition. N-formyl-methiongl-leucy-phenylalanine (fMLP)-or platelet-activating factor (PAF)-stimulated PMN strongly induced platelet aggregation, and the induction effect of PMN suspension was more active than that of PMN supernatant. Aspirin had no significant inhibitory effect on platelet aggregation induced by fMLP-or PAF-activated PMN. CONCLUSIONS: Different conditions of PMN (activated or non-activated) had the nearly opposite action on normal platelet reactivity. Briefly, non-activated-PMN inhibited platelet reactivity, whereas activated PMN stimulated it.