AIM: To prepare and characterize a novel anti-human CD83 monoclonal mAb. METHODS: Female BALB/c mice of 6-8 weeks old were immunized with CD83 transfectant (L929/CD83) as immunogen. The spleen B cells of the mice were fused with Sp2/0 myeloma cells. The hybridoma cells were screened with CD83 transfectant (L929/CD83 and 293/CD83) by FCM. The biological characteristics of antibody were investigated by rapid isotyping analysis, karyotype analysis, competitive inhibition test and Western blot. RESULTS: One hybridoma cell line named 9D8 was obtained, which had the property of secreting antihuman CD83 monoclonal antibody steadily, This mAb specifically recognized CD83 molecule expressed on human mature DC, activated T cells, and tumor cell line Daudi, myeloma cell line 8226. This mAb can recognize a distinct epitope from comercial mAb(HB15e). CONCLUSION: One hybndoma cell line has been developed successfully, which may lay a fundation for further study on the function of this molecule.

Objectives To establish the cell model of fibroblast in tumor and explore its malignant bionomics for providing valuable cell model for studying their transforming mechanism and developing drugs targeting the tumor stroma in the future. Methods The murine normal fibroblast cells, L 929 , were cultured with the supernatant of murine hepatocellular carcinoma cells, H 22 , for three and a half months, and L 929 -H 22 cells were acquired with stable growth. Then the changes of their biological characteristics were determined by light microscopy, electron microscopy, karyotype analysis, and MTT assay. The expressions of PCNA, bcl-2, MMP-9, TN, and tolomerase activity were detected by immunocytochemistry and RT-PCR, respectively. The ability to synthesize proteins and the effects of their supernatant on H 22 growth were determined. Results Abnormal nuclei and multinuclei were observed in L 929 -H 22 cells, and their chromatosome modes increased slightly. As compared with the parental cells, the population doubling time of L 929 -H 22 cells shortened (t=2.654 1, P

Objective To observe effects of Genistein and 5fluorouracil(5-FU) on human colon carcinoma cell line Colo320.Methods The MTT assay and median-effect principle were used.Results The two drugs were antagonistic at higher concentrations and synergistic at lower concentrations,The sequence and time of drug administration can influence the effects of the two drugs on Colo320.Conclusion The two drugs were cooperated at lower concentrations and antagonistic at higher concentrations.The sequence and time of drug administration were also important for their effects on the cells.

Objective To explore the relationship between posterior reversible encephalopathy syndrome (PRES) and the treatment of immunosuppressants such as cyclosporine A (CsA) and tacrolimus (FK506) in children with nephrotic syndrome.Methods The clinical data of nephrotic syndrome children with PRES caused by immunosuppressants who were hospitalized in Guangzhou First People's Hospital from June 2014 to May 2017 were collected.Their clinical characteristics,imaging features,treatments and prognosis were analyzed.Results A total of 23 children were enrolled,including 13 children with CsA and 10 children with FK506.In the concurrent of PRES 20 cases were in the activity stage of nephrotic syndrome,with large amounts of urinary protein,obvious edema,hypoalbuminemia and hyperlipidemia;while 3 cases were in the remission of nephrotic syndrome.The main clinical symptoms of PRES were hypertension,headache,epileptic attack,consciousness disorder,visual disorder and so on.Sixty-nine point six percent of children were using high dose immunosuppressive agents,and 78.3％ had high drug concentration.The cranial magnetic resonance imaging (MRI) results of 17 patients showed that they had T1 weighted (T1WI) hypointense,T2 weighted (T2WI) and fluid-attenuated inversion recovery (FLAIR) images hyperintense,as well as iso-and slight hypointense of diffusion-weighted image (DWI) in parietal-occipital regions or complicated with frontal lobes or basal nuclei region.Computer tomography (CT) examinations of 6 cases showed low-density focus of the occipital lobes.Children were relieved muscular spasm,debased intracranial hypertension,improved circulation,discontinued or reduced immunosuppressants at the onset of PRES.After these treatments,21 patients' symptoms and signs disappeared within one week;two patients suffered convulsions 2 times in one week,but recovered after one month.After three months 5 children had MRI and CT re-examination and it showed that their brain lesions disappeared.Conclusions PRES may be related to the dose and blood concentration of immunosuppressive agents.The immunosuppressants for nephrotic syndrome children should be increased gradually with low initiating doses.Physicians need to be precautious to prevent the occurrence of PRES once neurological symptoms occur.