Objective To research the association between the prognosis of Acinetobacter baumannii infection and drug resistance among patients in the intensive care unit( ICU). Methods Eighty-four patients with Acine tobacter baumannii infection from February 2003 to April 2009 in Peking University Shenzhen Hospital ICU were retrospectively analyzed and divided into alive group(59 patients) and dead group(25 patients) by using 30-day mortality. The impact of drug resistant Acinetobacter baumannii on mortality was assessed by binary logistic regression. Results The mortality of patients with Acinetobacter baumannii infection was related to age, haematological malignancy, the Chanson Comorbidity Index, APACHEII score, multidrug-resistant Acinetobacter baumannii ( MDRAB) , carbapenem-resistant Acinetobacter baumannii (CRAB ) , septic shock and antimicrobial inappropriate therapy by univariate analysis( P < 0.05). Binary logistic regression revealed that CRAB( OR = 3. 360,95%CI: 1. 563 ～7.223,P< 0.05)and the Charison Comorbidity Index( OR = 1. 633,95% CI: 1. 201 ～2. 221 ,P <0.05) were independent risk factors. Conclusions The prognosis of Acinetobacter baumannii infection was closely correlated with drug resistance. The patients with CRAB infection had a higher mortality.

[Objective] To investigate the improvement of abdominal symptoms,quality of life,and depression of Soothing Liver Regulating Qi and Regulating Spirit and Relieving Depression massage combined with western medicine treating diarrhea type irritable bowel syndrome(IBS) patients.[Methods] From January 2015 to December 2016,a total of 60 diarrhea type IBS patients were randomly divided into control group and treatment group.The control group was treated with Trimebutine Maleate Dispersible Tablets,while the treatment group was treated with Soothing Liver Regulating Qi and Regulating Spirit and Relieving Depression massage on the base of the control group.Two groups patients were treated for 4 weeks.The abdominal symptoms,quality of life,depression and serum 5-HT were collected before and after treatment.[Result] After 4 weeks treatment,the abdominal pain/abdominal discomfort,diarrhea,HAMD of both groups were lower than before treatment,and the treatment group was lower than the control group(P＜0.05).The SF-36 of both groups were higher than before treatment,and the treatment group was higher than the control group(P＜0.05).Serum 5-HT of both groups were lower than before treatment,and the treatment group was lower than the control group(P＜0.05).[Conclusion] Soothing Liver Regulating Qi and Regulating Spirit and Relieving Depression massage combined with western medicine can effectively improve the abdominal symptoms and quality of life for diarrhea type IBS patients.

Objective To assess antibiotic prescription habits,cost pattern and the prospective intervention in Intensive Care Unit was analyzed.Methods Data on antibiotic utilization and antibiotics susceptibility were col-lected prospectively from individual electronic charts from July 2014 to September 2014.Results 225 of 246 patients surveyed used antimicrobial during the ICU stay,and antibacterial drug utilization rate was 91.46%.Cefperazone-sulbactam and piperacillin-tazobactam were the most prescribed medications.Total defined daily dose ( DDDs) was 1121.1 DDDs.Bacteria culture was 98 positive in 677 cases and 101 pathogenic bacteria were isolated.Conclusion Interventional programs should focus on promoting infectious control with rational antibiotic prescription aimed at mini-mizing the future emergence of bacterial resistance and futile.

Objective To investigate the protective effects and mechanism of nitric oxide(NO) inhalation on rabbit lung ischemia/reperfusion injury. Methods Total 32 Japanese white rabbits were randomly divided into four groups, each group containing 8 rabbits. In group A, animals received only thoracotomy. In group B, after animals left chest were opened, their left main pulmonary arteries were occluded for 2h, then reperfused for another 2h. In group C, animal inhaled 20 ppm NO for 30min since the eleventh minute after reperfusion. In group D, animal inhaled 60ppm NO for 30min since the eleventh minutes after reperfusion. The levels of blood PaO 2, cGMP, slCAM-1, XOD and MDA were measured before operation and after reperfusion. The pathologic changes of left lung was observed. The wet/dry (W/D) weight ratio of left lung tissue was measured. Results Both PaO 2 and cGMP levels after reperfusion in group B were much lower than that in groups A, C and D(P

Objective To investigate the effect of dihydromyricetin on proliferation and apoptosis of breast cancer MCF-7 cells.Methods From March 2014 to February 2015,breast cancer MCF-7 cells were treated with 99% pure DMY as an inhibitor.MTT assay,flow cytometry and immunocytochemistry were used to analyze the proliferation,apoptosis and protein expression of breast cancer cell MCF-7.Results When the DMY concentration was higher than 20 μg/mL,the inhibitory effect appeared,but not good.When 40 and 80 μg/mL DMY were used,the proliferation of MCF-6 cells were significantly inhibited,and have different degrees of sensitivity to it.When DMY was 80 μg/mL,the IC50 was 226.9 μg/mL.The inhibition rate and IC50 were compared with 0 μg/mL DMY,there was significant difference(P<0.05).Indicating that the relatively high concentration of DMY could significantly improve the patient′s condition of breast cancer;MCF-6 cells treated with 40 and 80 μg/mL DMY could induce G2/M phase arrest in DMY with concentration greater than 20 μg/mL(P<0.01),and showed significant cell apoptosis death phenomenon.At the same time,G0/G1 phase was also blocked and S phase cells decreased significantly,the difference was significant(P<0.05).The positive rate of PCNA in breast cancer MCF-6 cells significantly decreased(P<0.01) when the DMY concentration was higher than 50 μg/mL,the positive rate was> 50%,especially in DMY with 80 μg/mL,the positive rate was 10.00%.Compared with 0 μg/mL DMY,the difference was significant(P<0.05).Conclusion The use of dihydromyricetin in breast cancer patients can effectively inhibit the rapid increase of cancer cells,accelerate apoptosis,slow down the patient′s condition,the effect is outstanding.

Objective To evaluate the effect of optimizing surgerical patients’handover procedures using tracking methodology.Methods Two hundred patients hospitalized between July 2011 and May 2012 were assigned in the control group,in which their handover was conduced conventionally.Another 200 patients between April 2012 to February 2012 were assigned in the observation group,where their handover was done by tracking methodology.The two groups were compared in terms of incidence of adverse events at handover,time for handover and satisfaction of medical staff.Result Compared with the control group,the study group showed significantly lower incidence of adverse events,shorter surgical handover time and higher satisfaction degree of medical staff(P<0.05).Conclusion The tracking methodology can help to optimize handover procedures and quality control management, reduce the incidence of adverse events and improve working efficiency.

Objective To explore the role of PI3K/AKT signaling pathway in total hepatic ischemia-reperfusion(I/R)-induced lung injury in rats.Methods This study was divided into 2 sub-projects.(1)36 rats were killed respectively at preischemia and after reperfusion,the lung tissue was then sampled.(2)12 rats were randomly divided into Wortmannin group and model control group. AKT,p-AKT protein expression,apoptotic cells and PCNA protein expression were tested respectively by Western blot,TUNEL and immunohistochemistry analysis.Results(1)Compared with that in preiscemia group,after I/R the apoptotic index (AI)was increased,the p-AKT/AKT ratio and PCNA-positive index showed bidirectional changes,and the histological changes were well identified.(2)p-AKT/AKT ratio showed a positive correlation with PCNA-positive index and a negative correlation with AI.(3)Compared with control group,the p-AKT/AKT ratio and PCNA-positive index were lower,histological changes was more significant and AI was higher in Wortmannin group. Conclusion PI3K/AKT signaling pathway had protective effect on lung injury induced by hepatic I/R,which was potentially mediated by anti-apoptosis and promoting proliferation.

A new multi-channel EEG signal acquisition system,which adopts Cypress's EZ-USB FX2 series USB as its micro-controller,is presented in this paper.The system employs AD620 and OP07 at the pre-amplifying stage and the following two amplifying stages respectively.Universal Series Bus(USB) technology is used to realize the real-time recording,displaying and tele-monitoring of EEG signal,which can also be stored in CF card after compression.The USB software developing tool is WinDriver.

Objective To investigate the perioperative management and video-assisted thoraco-scopic (VATS) extended thymectomy for myasthenia gravis ( MG ). Methods A total of 102 patients who received extended thymectomy for MG from June 2000 to August 2007 were divided into 2 groups by operative approach: 51 in the VATS group and 51 in the full median sternotomy group. The clinical features, including operation time, operation blood loss, post-operative drainage, inci-dence of crisis, hospital stay, and the 1-year total effective rate after the operation were compared. Complications were treated exactly and cholinesterase inhibitors were applied to control the symptom of MG before the operation. The whole thoracic gland and fat in the anterior mediastinum were removed. Steroid, cholinesterase inhibitors and plasmapheresis were used to prevent and cure masthemic crisis after the operation. Results No patients died in the operation and hospitalization. There was no sig-nificant difference in the operation time [(128.14±34.82) min vs. (130.46±28.71) min] and the 1-year total effective rate after the operation (85.8% vs. 87.2% )in the 2 groups(P>0.05).The operation blood loss, postoperative drainage, incidence of crisis, and postoperative hospital stay in the median sternotomy group were higher or longer than those in the VATS group [(93.77±21.64) mL vs. (45.42±10.96)mL,P<0.05; (174.65±21.64)mL vs. (101.33±28.76) mL,P <0.01;7.84% vs. 1.96%, P<0.05;(14.23±3.17)d vs. (8.37±1.18)d, P<0.05)]. Conclusion VATS extended thymectomy for myasthenia gravis is safe, less invasive and effective. Preoperative preparation and the perioperative managemant can decrease the incidence of crisis. The plasmapheresis is the key of curing masthemic crisis.

Objective To establish a simple,rapid,accurate and practical method for determination of chloride in drinking water by chloride ion-selective electrode.Methods The chlorine ion-selective electrode was used as indicator electrode and type217saturated calomel electrode of the double salt bridge(the lower salt bridge was filled with a solution of0.1mol/L potassium nitrate)was used as reference electrode.The contents of chloride in water were calculated using the calibration curve method in the electrolyte containing0.1mol/L potassium nitrate.Results The linear range of the method was10 -5 -10 0 mol/L and the best lin ear range was10 -4 -10 -1 mol/L with r=0.9999and the slope=53.5?0.667.The detection limit,recovery rate and coefficient of variation(CV)were0.3545mg /L,94.2%-102.2%(mean98.1%)and2.8%respectively.The suitable pH for the determination of the method was2-10.In the solution containing3.545mg/L chloride,50times more BrO -3 ,NO -2 ,CH 3 COO - ,SO 42- ,F - ,CrO 42- and10times more CO 32- could not interfere with the detection,but Br - and I - were interfering factors.The re-sults obtained by the chlorine ion-selective electrode method and the national standard determination method showed no statistic differences.Conclusion The method was easy to operate,rapid,accurate and suitable for determination of chloride in water.