1.Approach to the patient with pregnancy-associated fulminant type 1 diabetes mellitus
Weixia JIAN ; Xueru CHEN ; Weixing WANG ; Jie JIN ; Qing SU
Chinese Journal of Endocrinology and Metabolism 2013;29(8):719-721
One case with fulminant type 1 diabetes during the third trimester of pregnancy was reported.If a patient manifests abrupt onset of hyperglycemia,nausea,vomiting,and bellyache during pregnancy or immediately after delivery,fulminant type 1 diabetes should be considered.
2.Effects of advanced glycation end products on cell viability and level of reactive oxygen species(ROS)in MIN6 cells
Shichun DU ; Ning LIN ; Qinmin GE ; Weixia JIAN ; Yan DONG ; Qing SU
Chinese Journal of Endocrinology and Metabolism 2011;27(2):152-154
To explore the effect of advanced glycation end-products(AGEs)on cell viability and level of reactive oxygen species(ROS)in MIN6 cells. After intervention of various concentrations(100,200, and 400 mg/L)of AGEs for some time, cell viability was detected by MTT assay. 2', 7'-dichlorofluorescein diacetate(DCFH-DA)was used as a reactive oxygen species capture agent. The fluorescent intensity of 2', 7'-dichlorofluorescein(DCF), which was the product of cellular oxidation of DCFH-DA, was detected by flow cytometry. The level of ROS and insulin secretion was thus measured. Viability of MIN6 cells was inhibited by AGEs in a dose and time dependent manner(P<0.05).Intracellular fluorescent intensity of DCF was markedly elevated in the AGEs groups as compared with that in the control group(P<0.05).Insulin secretion was decreased in the AGEs groups than that in the control group(P>0.05). The results suggest that AGEs inhibit the viability and induce oxidative stress in MIN6 cells by overproduction of ROS.
3.Approach to the patient with vitamin D-deficient osteomalacia due to X-linked agammaglobulinemia
Xueru CHEN ; Yixin NIU ; Weixia JIAN ; Qing SU
Chinese Journal of Endocrinology and Metabolism 2019;35(8):711-714
Osteomalacia is a metabolic bone disease characterized by impaired mineralization of bone matrix. VitaminD deficiency contributes to a decrease in the efficiency of intestinal calcium and phosphorus absorption, resulting in secondary hyperparathyroidism and an inadequate calcium-phosphorus product, thereby causing osteomalacia. We present a patient who was diagnosed as vitamin D-deficient osteomalacia due to X-linked agammaglobulinemia ( XLA) , and the genetic analysis of the BTK gene revealed a missense mutation ( c.82C>T) . It should be attached great importance to etiological analysis of osteomalacia, and XLA may also be a cause of vitamin D deficiency.