Objective To investigate the expression of sex hormone receptor (SHR) in meningioma and its relationships to the origin of tumor central artery.Methods The expressions of sex hormone receptors, including progesterone receptor (PR), androgen receptor (AR) and estrogen receptor (ER) in 43 cases with meningioma were detected by immunohistochemically streptavidin-peroxidase (SP) method. These data combined with the characteristics of digital subtraction angiography (DSA) were analyzed.Results The expression of ER in meningioma was not related to the origin of tumor central artery. The positive expression rates of PR and AR in external carotid artery (ECA) original group (26.9%,23.1%) were significantly lower than those in internal carotid artery (ICA) original group (88.9%,77.8%) and ECA combined with ICA group (87.5%,87.5%) (all P

Although the treatment of aneurysmal subarachnoid hemorrhage (SAH) has made considerable progress in recent years,its mortality and disability have not been effectively controlled.Ischemic brain injury after SAH is considered to be an important reason for the poor prognosis of patients; however,its mechanism is still not fully understood.Studies have shown that the cerebral microcirculation disturbance after SAH is closely associated with ischemic brain injury.This article reviews the advances in research on cerebral microcirculation disturbance and ischemic brain injury after SAH.

Objective To summarize the method of forearm skin flap with dorsal interosseous artery placed by turning the proximal end of the flap to the distal side of the wound to repair skin and soft tissue defect of palm and thumb of hand,and put forward the key points of operation.Methods From 2008 to 2013,the forearm skin flap with interosseous dorsal artery to repair skin and soft tissue defect of palm and the part between thumb and index finger in 20 cases.Dorsal interosseous artery in the forearm is passing through between superficial and deep layers of extensor muscles and sends out 5-13 cutaneous branches to dorsal antebrachium skin.The large skin flap has good blood supply with high survival rate.Results All flaps survived,and good results were achieved in all 20 cases.Conclusion The flap preserved two main arteries from upper limb with good blood supply,and the amount of blood supply of the flap depended on the size of flap,and the wound of donor site can be directly sutured up or covered with free skin graft,thus minimizing the alteration of appearance and function of donor area.

Flow cytometry (FCM) is one of the most advanced cell quantitative analysis technology.Today,flow cytometry has been extensively and intensively used in medicine and other science,ranging from basic research to clinical diagnosis With the rapid development of science and technology of China,the application of flow cytometry in clinical research and diagnosis has also made significant progress.

Objective To perform the serological identification of anti‐E and anti‐Mur found in the detection before blood trans‐fusion and to analyze its clinical significance in blood transfusion .Methods The reaction pattern of serum with the antibody screen‐ing spectrum was detected by using the micro‐column gel method for identifying the antibody type and specificity .Results The 3 kinds of antibody anti‐E IgG combined with anti‐Mur IgG + IgM existed in the serum of 2 patients .Conclusion Anti‐E and anti‐Mur all are easier to lead to the hemolytic blood transfusion reaction .Selecting the suitable donor on the basis of the accurate anti‐body identification can provide guarantee for blood transfusion safety .

Objective To evaluate the application of Erytra automatic blood analyzer (Erytra) on blood grouping and cross matc‐hing .Methods 9 860 cases of EDTA‐K2 anticoagulant specimens for blood grouping test and 5 099 cases of EDTA‐K2 anticoagu‐lant specimens for cross matching were both detected by Erytra and manual tube method .Results There was no significant differ‐ence in the accuracy of blood grouping detected by Erytra and manual tube method (P>0 .05) .Both of the positive rate and the false positive rate of cross matching detected by Erytra were higher than those detected by manual tube method .Conclusion Erytra automatic blood analyzer can be applied for the detection of blood grouping and cross matching ,and its performance can meet the needs of blood safety in clinic .

Over the past several years, mass spectrometry technology has become the important method of choice for the discovery of new biomarkers.Because the features of mass spectrometry-based proteomics including sensitivity, high throughput, speed, combined with advanced bioinformatics allow for the rapid analysis of a bunch of proteins simultaneously.It has become a powerful laboratory tool in clinical study.However recent studies showed that critical comments were made on the poor reproducibility,statistical analysis of the data et al.This article focused on challenges of study design, mass spectrometry technology and biological relevance associated its application of mass spectrometry based proteomics in serum or plasma.

In recent years，the repair and regeneration of peripheral nerve injury has developed. This paper reviewed the researches of peripheral nerve injury repairing from the low, medium, and high frequency electric therapy. The electric therapies should be selected with the pathology and regeneration of the injured peripheral nerve. The mechanism need further study.

Objective To identify the possible mutations other than A1555G in mitochondrial 12S and 16S rRNA genes that responsible for aminoglycoside-induced deafness, and to provide the basis for genetic diagnosis for this disease. Methods A total of twenty-seven blood samples were obtained from five families with aminoglycoside-induced deafness for screening the A1555G mutation and other possible mutations by PCR- Alw26 I digestion and sequence analysis. Results All samples examined in four families (A, C, D and E) carried the same homoplasmic A1555G mutation, but no A1555G mutation was found in family B. Sequencing of the DNA samples from this family displayed a rare insertion of "AA" at nucleotide 2227 in 16S rRNA gene. Conclusions Our findings suggested that the 1555 G mutation was not the only genetic defect associated with aminoglycoside-induced deafness since we did not find the A1555G mutation in one family, in which the typical maternal inheritance pattern of the aminoglycoside-induced deafness was seen. It is not enough to identify prospectively patients who are likely to be hypersensitive to aminoglycoside ototoxicity by screening A1555G mutation only. Other possible mutations in mitochondrial DNA that associated with aminoglycoside -induced deafness should be tested also.

AIM: To explore the different inhibitory effect of arsenic trioxide (As_2O_3) on hepatocarcinoma cell growth in SMMC-7721 and BEL-7402 cell lines and its mechanism. METHODS: The cell culture and trypan blue staining were used to study the inhibitory effect of arsenic trioxide on cell growth, and the glutathione (GSH) contents in hepatocarcinoma cells treated with arsenic trioxide were detected. RESULTS: Arsenic trioxide inhibited the growth of BEL-7402 cells in a time and dose-dependent manner. The inhibitory effect was significant at a lower dose of 0.50 ?mol/L for 24 h, however, to SMMC-7721 cells, a higher dose of 2.00 ?mol/L for 96 h was needed. The inhibitory rate of arsenic trioxide (0.25-2.00 ?mol/L) on BEL-7402 cell growth was higher than that on SMMC-7721 cells. The content of GSH in SMMC-7721 cells was much higher than that in BEL-7402 cells [(50.8?5.2) (?mol/g) protein and (18.7?1.4) ?mol/g protein, respectively]. CONCLUSION: There was a significant difference in inhibition of hepatocarcinoma cell growth by arsenic trioxide between BEL-7402 and SMMC-7721 cell lines, the cause of which may be due to the difference in GSH content in BEL-7402 and SMMC-7721 cells. [