Objective:To obtain the stem cells in different pancreatic islets purity utilizing free-serum culture medium and investigate the PDX-1 mRNA expression distinction before and after transdifferentiation culture.Methods:Pancreatic islets of male SD rats(islet donors)were isolated by injection of collagenase into pancreatic ducts,and maintained in serum Ham's F-10 for 36h.Then the cells were kept in free-serum Ham's F-10 for 14 days.The last stage was to shift culture media from free-serum Ham's F-10 to free-serum DMEM/F12 that contained KGF,BSA,glucose and ITS.On the 28d,Dithizon was added into the culture flask for observation of 3D cell.The PDX-1 positive cells was investigated by immunocytochemistry.Results:After transdifferentiation culture,some 3D cells were red.The pdx-1 was remarkable in cell nucleus.Conclusion:After transdifferentiation culture,some pancreatic stem cell display the character of islet cell.The daughter cells of pancreatic stem cells have character of stem cells after transdifferentiation.KGF may have effect on promoting the expression of PDX-1.

Objective To investigate the vulvaginal candidiasis(VVC)incidence in pregnant women,identify the candida species and analyze the drug resistance of isolated strains.Methods Candida isolation,sample cultivation,species identification and drug susceptibility test were performed for VVC patients.Seven hundred and forty-five samples were collected.Results The incidence of VVC was 10.20%,and the recurrence of VVC was 18.5%.The proportion of C.albicans,C.glabrata,C.krusei,C.tropicalis and other candida were 80.26%,8.95%,3.95%,1.32% and 6.58% respectively.C.albicans was susceptible to amphotericin and 5-flucytosine,the susceptibility for fluconzazole,itroconazole and voriconazole were 7.89%,6.58% and 9.21% respectively.C.gla-brata,C.krusei,C.tropicalis,and other candida were susceptible to those antifungal drugs.Conclusion VVC was an usual infection among pregnant women,C.albicans was the major species,followed by C.glabrata.The resistance of amphotericin and 5-flucytosine were lower,the Candida were resistant to fluconzazole,itroconazole and voriconazole in certain extent.

The construction ofteachingteamin institutes is an effective wayfor organizingthe basic teaching constitution and improving teaching quality.We obtained the excellent teaching team of Chongqing in 2008.Our practice and thinking will be introduced in this paper,so as to provide the reference for teaching team construction in institutes.

PBL teaching method can cultivate students’ ability to analyze the existing problems, find out more problems and resolve the problems finally, and it is important to improve students’ comprehensive quality. The experimental teaching innovation of Histology and Embryology is summarized in this article, which turned induction, practice and examination, the three stages of experimental teaching into" Problem-Based learning", thus making the teaching effect much better.

HIFU is a new technology of noninvasive tumorectomy, which take use of thermal effects, mechanical effects and cavitation effects, etc. Currently, One of the difficulties in clinical application is how to determine energy dose of HIFU and resulted biological change. This paper expounds the factors such as sound intensity, irradiation duration, treatment depth, sound environment,etc. ,which affect energy input and therapeutic efficiency in HIFU treatment.

Compared to the conventional two-dimensional (2D) culture,in vitro three-dimensional cell culture (3DCC) can mimic the microenvironment more exactly.Compared to in vivo animal models,3DCC is less time-consuming and can be real-time recorded.Thus,3DCC fills the gap between 2D culture and animal models,which is used for oncologic research quickly.In 3DCC,the characteristics of cancer cells,such as morphology,the capability of invasion and migration and the resistance to chemo-radiotherapy,are quite different from those in 2D culture.The development of 3DCC technology provides a platform for oncology,organization engineering and gene or drug screening.

The enzymatic activity of monocytes was studied with light microscopic semi-quantitive analysis and microspectrophotometer, including B-glucuronidase, acid phosphatase, N-acetyl-?-glucosaminidase, peroxidase and ?-naphthyl acetate esterase in peripheral blood smears prepared by hcparinazed micropipet centrifugal method in some normal donors and patients. The data of normal groups have been obtained. The results tested by microspectrophotometry have indicated that the larger the area of monocyte was, the stronger the five kinds of enzymatic activity in monocyte was. In comparison with the normal groups, there were significant alterations of the different kinds of enzymatic activity of monocytes in the patients with cir- rhosis, systemic lupus erythematosus, pemphigus, untreated malignant tumor and radiotheraped tumor respectively. The significance of the results described above have been discussed in the paper.

Objective To design and implement an Android -based medical chest system to realize informatized management of military medicine and equipment.Methods RFID tag was attached to the materials in the chest, and reading module with multiple antenna, Bluetooth module and responder were installed on the chest, Android-based PDA was developed to realize information communication.Results Trials proved that the system could perform auto classified management and precision locating of the materials in the chest, and could provide direction for medicine taking.Conclusion The system enhances the efficiency of the staff and ensures the accuracy and safety of medicine taking, and thus can be popularized in military hospitals.

BACKGROUND: It has been found that hypoxia inducible 1α (HIF1α) gene can improve the ability of tissues and cells to survive in the ischemic environment, which is of great significance in promoting blood vessel regeneration.OBJECTIVE: To investigate the effect of the recombinant adenovirus Ad-CMV-HIF1αmu-IRES-hrGFP-1 on the proliferation of bone marrow mesenchymal stem cells.METHODS: Passage 3 bone marrow mesenchymal stem cells were cultured and transfected with Ad-CMV-HIF1αmu-IRES-hrGFP-1 virus (experimental group), with non-mutated gene Ad-CMV-HIF1α-IRES-hrGFP (positive control group), with Ad-CMV-IRES-hrGFP-1 empty virus (negative control group), or with no virus solution (blank control group). After transfection for 24 hours, the expression of HIF1α mRNA and protein was detected. Cell proliferation was detected by MTT assay at 24, 48, 72, 96 and 120 hours after transfection.RESULTS AND CONCLUSION: (1) Except that the mRNA expression of HIF1α in the experimental group and the positive control group was significantly higher than that in the negative control group and the blank control group (P < 0.05), there was no significant difference between two groups. (2) The expression of HIF1α protein in the experimental group was significantly higher than that in the positive control group, the negative control group and the blank control group (P < 0.05), and there was no significant difference between the latter three groups. (3) Cell proliferation was faster in the experimental group than the positive control group and the blank control group (P < 0.05), and moreover, there was no significant difference between the latter two groups. Our experimental findings indicate that the recombinant adenovirus-mediated three-point mutant HIF1α combined with hrGFP gene transfected bone marrow mesenchymal stem cells could not only express the target protein, HIF1α, under normoxic conditions, but also promote the proliferation of bone marrow mesenchymal stem cells.

Objective To study the effect of different insulin administration methods on glucose control of gestational diabetes mellitus ( GDM) .Methods Sixty-four cases with GDM were randomly divided into observation group and control group.The observation group received continuous subcutaneous insulin infusion insulin pump therapy, the control group received multiple subcutaneous insulin therapy.Then,the glucose control effect,SAS and SDS score were compared between two groups.Results After 4 weeks,the FBG,HbA1c,2h PG of observation group were significantly lower than the control group[(5.89 ±0.66)mmol/L vs (7.13 ±0.74)mmol/L,(7.14 ±0.82)% vs (8.92 ±0.95)%,(8.87 ±0.92)mmol/L vs (10.13 ±1.32)mmol/L](t=7.074,4.430,8.024,all P<0.05). The glucose fluctuations,insulin amount,hypoglycemia incidence of the observation group were significantly lower than the control group,blood sugar standard time was shorter than the control group[(5.64 ±0.65)mmol/L vs (8.44 ± 0.92)mmol/L,(0.52 ±0.06)U· kg-1· d-1 vs (0.73 ±0.09)U· kg-1· d-1,6.25% vs 28.13%,(4.76 ± 0.55)d vs (7.41 ±0.94)d,t/χ2 =14.061,10.983,5.379,13.764,all P<0.05)];SAS score,SDS score of the observation group were significantly lower than the control group[(34.95 ±4.2) points vs (40.23 ±3.98) points, (35.12 ±3.95)points vs (38.36 ±3.88)points,t=5.162,3.310,all P<0.05)].Conclusion Continuous subcu-taneous insulin infusion insulin pump therapy is helpful to control glucose levels,reduce blood sugar fluctuations and mitigate negative sentiment during treatment,it is an ideal insulin administration method for the treatment of GDM.