Objective:To obtain the stem cells in different pancreatic islets purity utilizing free-serum culture medium and investigate the PDX-1 mRNA expression distinction before and after transdifferentiation culture.Methods:Pancreatic islets of male SD rats(islet donors)were isolated by injection of collagenase into pancreatic ducts,and maintained in serum Ham's F-10 for 36h.Then the cells were kept in free-serum Ham's F-10 for 14 days.The last stage was to shift culture media from free-serum Ham's F-10 to free-serum DMEM/F12 that contained KGF,BSA,glucose and ITS.On the 28d,Dithizon was added into the culture flask for observation of 3D cell.The PDX-1 positive cells was investigated by immunocytochemistry.Results:After transdifferentiation culture,some 3D cells were red.The pdx-1 was remarkable in cell nucleus.Conclusion:After transdifferentiation culture,some pancreatic stem cell display the character of islet cell.The daughter cells of pancreatic stem cells have character of stem cells after transdifferentiation.KGF may have effect on promoting the expression of PDX-1.

PBL teaching method can cultivate students’ ability to analyze the existing problems, find out more problems and resolve the problems finally, and it is important to improve students’ comprehensive quality. The experimental teaching innovation of Histology and Embryology is summarized in this article, which turned induction, practice and examination, the three stages of experimental teaching into" Problem-Based learning", thus making the teaching effect much better.

The construction ofteachingteamin institutes is an effective wayfor organizingthe basic teaching constitution and improving teaching quality.We obtained the excellent teaching team of Chongqing in 2008.Our practice and thinking will be introduced in this paper,so as to provide the reference for teaching team construction in institutes.

Objective To investigate the vulvaginal candidiasis(VVC)incidence in pregnant women,identify the candida species and analyze the drug resistance of isolated strains.Methods Candida isolation,sample cultivation,species identification and drug susceptibility test were performed for VVC patients.Seven hundred and forty-five samples were collected.Results The incidence of VVC was 10.20%,and the recurrence of VVC was 18.5%.The proportion of C.albicans,C.glabrata,C.krusei,C.tropicalis and other candida were 80.26%,8.95%,3.95%,1.32% and 6.58% respectively.C.albicans was susceptible to amphotericin and 5-flucytosine,the susceptibility for fluconzazole,itroconazole and voriconazole were 7.89%,6.58% and 9.21% respectively.C.gla-brata,C.krusei,C.tropicalis,and other candida were susceptible to those antifungal drugs.Conclusion VVC was an usual infection among pregnant women,C.albicans was the major species,followed by C.glabrata.The resistance of amphotericin and 5-flucytosine were lower,the Candida were resistant to fluconzazole,itroconazole and voriconazole in certain extent.

HIFU is a new technology of noninvasive tumorectomy, which take use of thermal effects, mechanical effects and cavitation effects, etc. Currently, One of the difficulties in clinical application is how to determine energy dose of HIFU and resulted biological change. This paper expounds the factors such as sound intensity, irradiation duration, treatment depth, sound environment,etc. ,which affect energy input and therapeutic efficiency in HIFU treatment.

Objective To observe the effect on serum inflammatory factors and immune function of Shiwei Longdanhua particles in elderly patients with acute bronchitis.Methods 120 cases of elderly patients with acute bronchitis from November 2014 to October 2016 were selected and randomly divided into the observation group and the control group with 60 cases in each group, according the order of single and double number.The control group was given conventional treatment, and the observation group was given Shiwei Longdanhua particles based on the control group.Treatment for seven days, to observe the differences of serum inflammatory factors (hs-CRP, TNF-αand IL-6, IL-1) and immunologic function indexes (CD3 +, CD4 +, CD8 +, CD4 +/CD8 +and IgM, IgG, IgA) between the two groups.Results After treatment, hs-CRP, TNF-αand IL-6, IL-1 of the two groups decreased with before treatment, the difference was statistically significant (P<0.05), and all indexes of the observation group were lower than those of the control group, the difference was statistically significant ( P<0.05 ) .After treatment, CD3 +, CD4 +, CD4 +/CD8 + of the two groups increased with before treatment except CD8 +decreased, the difference was statistically significant (P<0.05), there was no significant difference between the control group and before treatment, there was significant difference between the two groups after treatment (P<0.05).After treatment, IgM, IgG and IgA of the two groups decreased with before treatment, the difference was statistically significant (P<0.05), and all indexes of the observation group were lower than those of the control group, with significant differences, the difference was statistically significant (P<0.05).Conclusion Shiwei Longdanhua particles has the effects of inhibiting inflammatory reaction and improving the immune status in the treatment of elderly patients with acute bronchitis .

Objective To investigate the effects of combination of aniso-dosage of Astragalus and Arctium on the glycolipin metabolism and renal lesion in diabetic rats.Method Streptozotocin (STZ)-induced diabetic rats were established as experimental model.The diabetic rats were randomized into model group and mixture-treated group.Astragalus and Arctium were divided into three dosages (low,moderate,high) respectively and combined according to orthogonal experimental design and were administrated to the mixture-treated group.At the 4th and 8th week,parameters of blood,urine and renal tissue were examined.The blood sugar,cholesterol,triglycerol,HbA1c were detected by analysis meter of biochemistry,urine microalbumin by radioimmunoassay (RIA) and 24 h urine protein by colorimetry.The HE and PAS staining were used to observe the change of glomerular ECM.Results At the 4th weekend,the combination of Arctium high-dosage had better effects on decreasing blood sugar and cholesterol.While at the 8th weekend,the effects of mixture were gradually augment with the Astragalus dosage increasing on decreasing triglycerol,24 h urine protein and ECM.Conclusion The compatibility of aniso-dosage of Astragalus and Arctium could rectify the metabolic disturbance of glycometabolism and lipid metabolism,and lessen the renal lesions.The compatibility had different effects on various experiment phases.

In view of the possibility for serious adverse outcomes for mother and fetus associated with maternal thyroid dysfunctions during pregnancy,many experts have recommended conducting routine thyroid function screening in pregnancy,but guidelines from different academic communities have given varied recommendations.The aim of this review is to discuss the significance of thyroid function screening in pregnancy and evaluate efficiency of the case-finding strategy vs.the universal screening strategy.

Compared to the conventional two-dimensional (2D) culture,in vitro three-dimensional cell culture (3DCC) can mimic the microenvironment more exactly.Compared to in vivo animal models,3DCC is less time-consuming and can be real-time recorded.Thus,3DCC fills the gap between 2D culture and animal models,which is used for oncologic research quickly.In 3DCC,the characteristics of cancer cells,such as morphology,the capability of invasion and migration and the resistance to chemo-radiotherapy,are quite different from those in 2D culture.The development of 3DCC technology provides a platform for oncology,organization engineering and gene or drug screening.

It is very difficult to operate the patients with malignant neoplasms combined with superior vena cava syndrome because of SVC has a special place.And it's prognosis is not good. Now we will use some relative articles to draw a synoptic conclusion in the indications ,surgical skills, and curative effects of this operation.