1.Effects of Astragaloside Ⅳ Derivative on Heart Failure in Rats
Weiting WANG ; Zhuanyou ZHAO ; Yingmei HAN ; Weiren XU ; Lida TANG
Chinese Herbal Medicines 2010;2(1):48-53
Objective Astrgaloside Ⅳ derivative (ASId) is one of Astragaloside Ⅳ (ASI) derivatives with higher water-solubility and may have more druggability than ASI. The present study aims at observing the effects of ASId on cardiovascular parameters in chronic heart failure in rats. Methods Using echocardiographic and haemodynamic measurements, the effects of ASId on congestive heart failure (CHF) induced by ligation of the left coronary artery in rats were investigated.ventricle (LV) pressure (dp/dt) in ASId treated groups were significantly increased. Both LV volumes in diastole and in systole were decreased significantly after ASId treatment, accompanied with a trend towards normalization of relative stress. ASId treatment also inhibited compensatory hypertrophy of depressed heart. Conclusion ASId could improve cardiac functions and inhibite compensatory hypertrophy and LV remodelling, which suggests the possibility of ASId as a new therapeutic drug for the treatment of CHF.
2.Protection of Astragaloside Derivate on Oxidative Stress and Hypertrophy in Cardiomyocytes
Chunhua HAO ; Weiting WANG ; Zhuanyou ZHAO ; Lida TANG
Chinese Herbal Medicines 2011;03(1):54-59
Objective The astragaloside Ⅳ(ASI)has been proved to play an important role in protecting against cell death on cardiovascular diseases.This study aims to investigate the effect of the astragaloside derivate.(ASId)on confronting oxidative stress and hypertrophy in myocardial cells.Methods Following exposure embryonic rat cardiac H9c2 cells to hydrogen peroxide(H2O2)and angiotensin Ⅱ for developing oxidative stress and hypertrophy,ASId at final concentrations(0.1,1,and 10 μmol/L)was added to study its role in protecting cardiomyocytes by biochemical detection and cell size measurement In addition,the mitochondrial permeability transition pore(mPTP)opener atractyloside(20 μmol/L)and inhibitor cyclosporin A(CSA)(1 μmol/L)were employed to investigate the possible mechanisms for anti-oxidation.Results ASId at 1 and 10 μmol/L in cultures suppressed oxidative stress at different degrees,which induced the decrease in LDH activity and MDA content,and also the increase in SOD activity in comparable with the model group; The mPTP opener atractyloside and inhibitor CSA weakened and strengthened the role of ASId,respectively.ASId at 10 μmol/L inhibited cell hypertrophy,and the cell diameter,surface area,and protein content were all decreased in comparable of those cells in model group.Conclusion ASId is involved in the cytoprotective effects on oxidative stress through a pathway mediated by mPTP,and also has a protective effect against hypertrophy.
3.Therapeutic effects of Di'ao Xinxuekang on myocardial ischemia-reperfusion injury in rats
Haijuan ZHENG ; Weiting WANG ; Chunhua HAO ; Zhuanyou ZHAO ; Lida TANG
Tianjin Medical Journal 2015;43(5):491-495
Objective To observe the therapeutic effect of Di'ao Xinxuekang (DAXXK) on myocardial ischemia-reperfusion injury in rats, and to explore its mechanisms. Methods The myocardial ischemia-reperfusion injury model was established by the ligation of descending coronary artery in rats. Then animals after the modeling were randomized into model group, DAXXK-d (31.5 mg/kg) group, DAXXK-g (63.0 mg/kg) group and Diltiazem (24.8 mg/kg) group. A separate sham group was used as control. The treatment group was given DAXXK once a day for 7 days. Cardiac function and cardiac configuration were measured by color Doppler ultrasound diagnostic method. Hemodynamics was measured by Millar catheter method. The arterial oxygen saturation and blood oxygen pressure were measured by i-STAT 300 blood gas analyzer. Inflammatory cytokines interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, adhesion molecules VCAM-1, ICAM-1, and apoptosis-related protein Bcl-2, Bax were detected by ELISA. Myocardial apoptosis was measured using TUNEL method. Results Compared with model group, the left ventricular fractional shortening (FS), the systolic and diastolic function were improved, and the left ventricular pressure maximum rise/ fall rates (± LVdp/dtmax) were increased, in DAXXK group. DAXXK improved lung function, increased arterial oxygen pressure and oxygen content. The inflammatory cytokines IL-1β, IL-6, TNF-α and adhesion molecules ICAM-1 and VCAM-1 were decreased in DAXXK group. The myocardial swelling and inflammatory infiltration were relieved, myocardial apoptosis was reduced, the expression of Bcl-2 protein was increased and the expression of Bax protein was decreased in DAXXK group. Conclusion DAXXK can protect myocardial ischemia-reperfusion injury, which involved in the inhibition of apoptosis and reduction of inflammatory cytokines.
4.Effects of YAP-small interfering RNA on the proliferation and apoptosis of human periodontal ligament stem cells.
Cuizhu TANG ; Yong WEN ; Weiting GU ; Bing ZHANG ; Yunpeng ZHANG ; Yawen JI ; Xin XU
West China Journal of Stomatology 2015;33(6):622-626
OBJECTIVETo investigate the effects of small interfering RNA (siRNA) targeting YAP on the proliferation and apoptosis of human periodontal ligament stem cells (hPDLSCs).
METHODSSynthesized sequences of siRNA were transfected into hPDLSCs by Lipofectamine™ 2000. The expression of YAP was identified by using real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis. Proliferation activity was detected by using cell counting kit-8 (CCK-8). Changes in the cell cycle and apoptosis rate were detected by using flow cytometry. Results were analyzed by using SPSS 19.0, and P < 0.05 was considered statistically significant.
RESULTSExpression of YAP mRNA and protein were significantly downregulated after 48 h of transfection (P < 0.001). No obvious difference was found in the expression levels of YAP protein between 48 and 72 h, thus indicating that siRNA could inhibit the expression of YAP persistently and effectively. Proliferation activity was inhibited, and apoptosis rate was increased. Cell cycle was changed as the proportion of G₁and S phases increased (P < 0.01) and G₂ phase decreased (P < 0.05).
CONCLUSIONKnocking down YAP gene by siRNA could inhibit proliferation activity, induce apoptosis, and change the cell cycle of hPDLSCs. Thus, YAP could regulate the proliferation and apoptosis of hPDLSCs.
Adaptor Proteins, Signal Transducing ; Apoptosis ; drug effects ; Cell Cycle ; drug effects ; Cell Proliferation ; drug effects ; Down-Regulation ; Humans ; Periodontal Ligament ; drug effects ; Phosphoproteins ; RNA, Messenger ; RNA, Small Interfering ; pharmacology ; Stem Cells ; drug effects ; Transfection
5.Application of 99TCm tracer technique in rabbit cerebral thromboembolic stroke
Chunhua HAO ; Xiangwei XU ; Yinzhong MA ; Rui ZHANG ; Shuangyong SUN ; Weiting WANG ; Zhuanyou ZHAO ; Lida TANG
Drug Evaluation Research 2017;40(5):648-651
Objective To study the application of 99Tcm in rabbit cerebral thromboembolic stroke and thrombolysis effect of recombinant tissue plasminogen activator (rt-PA).Methods The 0.5 mL radioactive pertechnetate sodium (specification:5 mCi/2mL and radiation intensity 92.5 MBq/mL) was combined with 30 μL stannous chloride (5 mg/mL),and the 20 μL mixture was joined to whole blood,red blood cells,and plasma for labelling.Then 50 μL CaCl2 (0.5 mol/L) and bovine thrombin (50 IU/mL) were doped in mixture,and rapidly sucked into a polyethylene plastic pipe (PE80).Thrombus was formed for 2 h at 37 ℃ and cut into small pieces of 10 mm.Autologous blood clots combined with 99Tcm from external carotid artery were injected to internal carotid artery of rabbit,the radioactivity (counts per minute,CPM) was measured by gamma counting instrument,and the improvement of rt-PA 4.5 mg/kg (clinical equivalent dose) on this model was observed.Results After thromboembolism,CPM increased approximately by (5.1 ± 1.3) times,which suggested that the model was reliable.The rt-PA 4.5 mg/kg had significant progressive thrombolysis effect.Conclusion 99Tcm tracer technology could be applied to rabbit cerebral stroke model,which is stable and reliable
6.Currence pathogenic mechanism for weight loss in Parkinson disease
Journal of Chinese Physician 2018;20(1):28-30,34
Weight loss is common in non-motor symptoms in Parkinson disease (PD).It may predate motor symptom onset,and may be associated with the development of disease.However,it was less accounted of clinically.The pathophysiology of weight loss in PD is very complicated.This review discusses the pathogenesis of PD weight loss from dopaminergic dysfunction,energy expenditure/intake imbalance,central mechanisms of feeding behavior regulation,and neuroendocrine abnormalities.
7.The 496th case:recurrent syncope for 8 years
Weiting TANG ; Yong YOU ; Shuangxing LI ; Bin ZHANG
Chinese Journal of Internal Medicine 2022;61(6):708-710
A 25-year-old women was admitted to the department of Neurology in Affiliated 2nd Hospital of Hainan Medical University due to recurrent syncope for 8 years and return for 2 months. She had multiple episodes of syncope at onset. She presented with the feeling of weakness in both lower limbs, and fatigue in the past year. She experienced pain in the waist and limbs joint in recent three months. Physical examination showed joint hyperactivity in metacarpophalangeal joints of both upper limbs, increased skin elasticity. Active-standing transcranial Doppler (TCD) test showed that the average heart rate (HR) and the average middle cerebral artery (MCA) blood flow velocity in the supine position were 79 beats/min and 62 cm/s, respectively; while the average HR and the average MCA blood flow velocity in the standing position were 126 beats/min, 47 cm/s. Meanwhile,the blood pressure was normal during the test of supine-to-standing TCD. Genetic testing indicated LDB3 transgenation. The patient was diagnosed as postural tachycardia syndrome (joint-hypermobility-related), Ehlers-Danlos syndrome, and relieved by fluid infusion and rehabilitation therapy.
8.A case of nasopharyngeal carcinoma with autonomic nervous dysfunction and literature review
Rui CHEN ; Shuying LI ; Weiting TANG ; Jingwei WU ; Yong YOU
Journal of Chinese Physician 2023;25(5):685-690
Objective:A case of advanced nasopharyngeal carcinoma with autonomic dysfunction was reported and its pathophysiological mechanism was discussed.Methods:The diagnosis and treatment of a nasopharyngeal carcinoma patient with autonomic nervous dysfunction such as paroxysmal syncope was summarized, and the pathophysiological mechanism of this case was analyzed by searching related literature.Results:Nasopharyngeal carcinoma characterized by autonomic dysfunction was rare and had a poor prognosis. Autonomic dysfunction caused by nasopharyngeal carcinoma was associated with carotid sinus syndrome, parapharyngeal space syncope syndrome, glossopharyngeal nerve reflex and paraneoplastic neuropathy.Conclusions:Early detection and treatment is a key factor affecting the prognosis of nasopharyngeal carcinoma. Clinicians should consider nasopharyngeal carcinoma as one of the differential diagnoses in the diagnosis and treatment of patients with autonomic nervous dysfunction combined with cranial nerve damage.
9.Analysis of prevalence,related factors of orthostatic intolerance in patients with Type 2 diabetes
Weiting TANG ; Shuangxing LI ; Shuying LI
Journal of Apoplexy and Nervous Diseases 2022;39(1):18-21
To explore the prevalence and related factors of orthostatic intolerance in patients with Type 2 diabetes. Methods Patients with Type 2 diabetes were enrolled,admitted to the Department of Neurology from September 2020 to October 2021.General clinical data were collected. The active standing TCD test were performed on all the patients,and the changes of blood pressure,heart rate and cerebral hemodynamics were recorded from supine to standing for 1 min,3 min,5 min and 10 min. Orthostatic intolerance questionnaire were completed. Association between Orthostatic intolerance questionnaire score and results of supine to standing TCD test were analyzed. Results A total of 161 patients were enrolled for this study,70 (43.5%) with Symptoms of orthostatic intolerance and 91 (56.5%) without. Correlation analysis showed that Orthostatic intolerance questionnaire score have positive correlation with the magnitude of systolic pressure fall,diastolic pressure fall,Cerebral blood flow velocity fall and cerebral blood flow response to tilt score.(r=0.57、P= 0.000,r=0.44、P=0.000,r=0.30、P=0.022,r=0.24、P=0.044).Conclusion The proportion of OI in patients with Type 2 diabetes is high,which is associated with orthostatic blood pressure and cerebral blood flow decline.