Objective To investigate pathogens and drug resistance of lower respiratory tract infection(LRTI)in Intensive Care Unit(ICU).Methods Retrospective study of the clinical data,the distribution and the drug-sensitivity of pathogens of 220 cases with LRTI in ICU.Results Totally 280 strains of pathogens were identified by bacterial culturing.The ratio of G-bacteria to total pathogens isolated was 63.5%,of the G+ bacteria was 25.1%,and of the fungi was 11.4%.The main kinds of the G-bacteria were Klebsiella pneumoniae(17.1%),Pseudomonas aeruginosa(13.2%),Acinetobacter baumannii(12.5%),and Stenotrophomonas maltophilia(10.4%).Staphylococcus aureus(SA)(91.4%)was the most prominent in G+ bacteria,and MRSA was 98.4% in SA.The result of drug sensitive test in vitro showed the multiple drug fast rate of Pseudomonas aeruginosa was comparatively high,Stenotrophomonas maltophilia to Levofloxacin was low,Klebsiella pneumoniae and Acinetobacter baumannii were highly sensitive to carbapenems.The susceptibility rate of MRSA to vancomycin was 100%.Conclusion G-bacteria are the majority of the pathogens,isolated from patients with LRTI in ICU.Klebsiella pneumoniae,Pseudomonas aeruginosa,Acinetobacter baumannii,and Stenotrophomonas maltophilia are the chief G-pathogens.Except Stenotrophomonas maltophilia,imipenem and merpenem are relatively active against the G-bacilli.The proportion of MRSA and fungal infection is increasing.It is suggested that there be urgent need for surveillance of bacterial resistance and rational use of antimicrobial agents during clinical therapy.

Objective To establish a loop-mediated isothermal amplification method for detection of Campylobacter jejuni.Methods Six sets of primers were designed to recognize Campylobacter jejuni specific gene hipO.One was selected as the optimal primer and its specificity and sensitivity to Campylobacter jejuni were evaluated by LAMP reaction in 60 minutes at 62℃.Results The results recorded by the turbidity meter showed that the sensitivity of LAMP with a detection limit of 6.97×102 copies/μl was ten times that of PCR.Conclusion LAMP is a potential and valuable method of detection of Campylobacter jejuni due to its rapidity,simplicity,low cost and accuracy.It is especially suitable for grass-roots medical units.

Collagen hydrogel is a new type of wound dressing. The combination of the advantages of collagen and hydrogel can effectively exert their respective advantages in wound repair and regeneration. In recent years, the use of physical and chemical method to improve the mechanical properties and biological functions of collagen hydrogels to make them more suitable for wound environment has become a hotspot in scientific research and clinical trials. This review systematically summarized the theoretical basis, application status and research progress of collagen hydrogel in wound repair, and analyzed the current advantages, limitations, and future research directions, to provide reference and directions for the development of collagen hydrogel and its clinical application in the field of wound repair.

Collagen hydrogel is a new type of wound dressing. The combination of the advantages of collagen and hydrogel can effectively exert their respective advantages in wound repair and regeneration. In recent years, the use of physical and chemical method to improve the mechanical properties and biological functions of collagen hydrogels to make them more suitable for wound environment has become a hotspot in scientific research and clinical trials. This review systematically summarized the theoretical basis, application status and research progress of collagen hydrogel in wound repair, and analyzed the current advantages, limitations, and future research directions, to provide reference and directions for the development of collagen hydrogel and its clinical application in the field of wound repair.

Collagen hydrogel is a new type of wound dressing. The combination of the advantages of collagen and hydrogel can effectively exert their respective advantages in wound repair and regeneration. In recent years, the use of physical and chemical method to improve the mechanical properties and biological functions of collagen hydrogels to make them more suitable for wound environment has become a hotspot in scientific research and clinical trials. This review systematically summarized the theoretical basis, application status and research progress of collagen hydrogel in wound repair, and analyzed the current advantages, limitations, and future research directions, to provide reference and directions for the development of collagen hydrogel and its clinical application in wound repair field.

Collagen hydrogel is a new type of wound dressing. The combination of the advantages of collagen and hydrogel can effectively exert their respective advantages in wound repair and regeneration. In recent years, the use of physical and chemical method to improve the mechanical properties and biological functions of collagen hydrogels to make them more suitable for wound environment has become a hotspot in scientific research and clinical trials. This review systematically summarized the theoretical basis, application status and research progress of collagen hydrogel in wound repair, and analyzed the current advantages, limitations, and future research directions, to provide reference and directions for the development of collagen hydrogel and its clinical application in wound repair field.

Objective To investigate the expression and significance of inducible nitric oxide synthase (iNOS), platelet-derived growth factor (PDGF)-B and lipopolysaccharide (LPS) in rat models with Budd-Chiari syndrome (BCS) . Methods BCS model was established by partial ligation of inferior vena cava in the posterior segment of the liver. The experimental rats were divided into control group (n=20), model group (n=20) and sham group (n=20) . Liver tissues were collected for immunohistochemistry, HE and Masson staining, and the expression levels of iNOS, PDGF-B and LPS were determined. Results The LPS value in model group was higher than that in both control group and sham group (P=0.001) . The mRNA and protein expressions of iNOS and PDGF-B in model group were higher than those in both control group and sham group (P=0.001) . Statistically significant differences in mRNA and protein expressions of iNOS and PDGF-B existed between each other among the subgroups (P=0.001) . In model group iNOS was positively correlated with PDGF-B and LPS; liver fibrosis was positively correlated with LPS and negatively correlated with PDGFB. Conclusion The damage and repair of BCS is a complicated process. The iNOS, PDGF-B and LPS may play different roles in different stages of BCS. How to regulate their balance in liver fibrosis may be a direction that deserves further study.

According to the pathological characteristics of symptomatic chronic thoracic and lumbar osteoporotic vertebral fracture (SCOVF), the different clinical treatment methods are selected, including vertebral augmentation, anterior-posterior fixation and fusion, posterior decompression fixation and fusion, and posterior correction osteotomy. However, there is still a lack of a unified understanding on how to choose appropriate treatment method for SCOVF. In order to reflect the new treatment concept and the evidence-based medicine progress of SCOVF in a timely manner and standardize its treatment, the clinical guideline for surgical treatment of SCOVF is formulated in compliance with the principle of scientificity, practicability and advancement and based on the level of evidence-based medicine.

The CyberKnife, an acronym for the stereotactic radiosurgery platform, represents an image-guided stereotactic radiotherapy technique. This technology precisely delivers ionizing radiation to tissues, effectively damaging tumor cells, and is suitable for radiotherapy of both intracranial and extracranial tumors. This article reports the first performance of CyberKnife by radiotherapy at Peking Union Medical College Hospital, including a patient with uncontrolled pituitary adenoma after surgery and radiotherapy, and another patient with vertebral metastasis following targeted therapy for lung adenocarcinoma. The application of CyberKnife technology in radiotherapy has achieved highly accurate dose delivery, enabling targeted irradiation of tumor lesions while minimizing damage to surrounding normal tissues, thereby yielding relatively ideal clinical outcomes.