The antiapoptotic mechanisms of cell transplantation have been paid wide attention in the process of the treatment of cerebral ischemic injtry.The transplanted cells play their roles of mtiapoptotic effect through releasing growth factors and nutritional factors,direct bonding,promoting angiogenesis,and anti-inflammation.

Objective To explore the curative effect of stereotactic conformal radiotherapy (SCR) for extracranial tumors. Methods Eighty-six patients with extracranial tumors underwent SCR from May 1997 to August 2002. The clinical target volume (CTV) of tumors ranged from 0.2 cm3 to 232 cm3, with a mean of 27.6 cm3. The prescribed dose to the planning target volume (PTV) was 3～12 Gy (mean,5.3 Gy),which were prescribed in 1～10 fractions. Results No patient died during the treatment. Karnofsky performance scores (KPS) ranged 10～90 (mean,60) and 20～100 (mean,82) before and after the treatment. Follow-up for 1～5 years showed complete remittence (CR) in 25 cases, partial remittence (PR) in 49 cases, no change in 9 cases, and progressed in 3 cases. Conclusions SCR is an effective method in the management of extracranial tumors.

Objective To study the chemical constituents from Selaginella sinensis.Methods The compounds were isolated with Diaion HP-20,Toyopearl HW-40,silica gel column chromatography.The structures of these compounds were identified by physicochemical properties and spectral analyses.Results Eleven compounds were isolated from the 70% acetone-extracts and their structures were identified as ?-sitosterol(Ⅰ),vanillic acid(Ⅱ),(7S,8R)-4,9,9′-trihydroxy-3,3′-dimethoxy-7,8-dihydrobenzofuran-1′-propylneolignan(Ⅲ),syringaresinol(Ⅳ),(-)-pinoresinol(Ⅴ),pinoresinol-4-O?-D-glucopyranoside(Ⅵ),syringaresinol-4,4′-O-di-?-D-glucopyranoside(Ⅶ),?-methylD-xylopyranoside(Ⅷ),?-methyl-D-arabinopyranoside(Ⅸ),hinokiflavone(Ⅹ),and amentoflavone(Ⅺ).Conclusion Compounds Ⅱ-Ⅸ are isolated from this plant for the first time.

OBJECTIVE:To establish a method for identification of Cornus officinalis and its Preparations. METHODS: LC-MS method was adopted. The separation was performed on Hypersil C18 column (150 mm?2.1 mm,5 ?m) with a mobile phase of methanol-acetic acid(84 ∶ 16) at a flow rate of 0.2 mL?min-1. The column temperature was set at 30℃. Mass spectra were equipped with an electrospray ionization (ESI) source with negative ion detection model as ion scanning. The conditions of determination were sheath gas flow rate of 1.5 L?min-1,sweep gas flow rate of 0.45 L?min-1,spraying voltage of 4.5 kV, high temperature capillary temperature of 350℃ and scan range of 110～600 amu. RESULTS: The three-level mass spectrometry of the ursolic acid and oleanolic acid was well separated. The results were stable. Three-level mass spectrometry of ursolic acid and oleanolic acid were established and identification of C. officinalis preparations was carried out.CONCLUSION:Three-level fragment peaks of fat-soluble constituents of C. officinalis can be used for indentification of C. officinalis and its preparations.

The aim of this study was to look for the chemical constituents from the rhizomes of Dryopteris sublaeta. The fresh plant was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 ℃. The concentrated material was partitioned with ether, ethyl acetate and n-butanol. The fraction of ethyl acetate was repeatedly chromatographied over silica gel and Sephadex LH-20 columns. Structures of pure compounds were established on the basis of their physiochemical and spectral data. Nine compounds were obtained and identified as sublaetentin A (1), sublaetentin B (2), sublaetentin C (3), sublaetentin D (4), matteuorienate A (5), matteuorienate C (6), arbutin (7), 3-methoxy-4-hydroxyphenyl-1-O-β-D-glucopyranoside (8) and 3,4-dimethoxyphenyl-1-O-β-D-glucopyranoside (9). Compounds 1-4 are new compounds, the others were isolated from this plant for the first time.

To study the chemical constituents from the leaves of Celastrus gemmatus Loes., chromatographic methods were used to isolate and purify the chemical constituents, their structures were elucidated by the physiochemical characteristics and spectral data. Nine compounds were obtained and identified as (-)-massoniresinol 3a-O-β-D-glucopyranoside (1), ambrosidine (2), isolariciresinol 9-O-β-D-glucopyranoside (3), kaempferol 3-O-β-D-glucopyranoside(astragalin) (4), kaempferol 3-O-rutinoside (5), kaempferol 3-O-neohesperidoside (6), apigenin 7-O-β-D-glucuronide (7), apigenin 7-O-β-D-glucuronide methyl ester (8) and D-sorbitol (9). Compound 1 is a new compound, the others are isolated from this genus for the first time, and this is the first time to report lignan compounds from genus Celastrus.

Aim To study the chemical constituents of Dryopteris sublaeta Ching et Hsu. Methods Fresh plant of Dryopteris sublaeta Ching et Hsu was extracted twice with boiling water, concentrated to small volume under reduced pressure at 50 ℃. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol. The fraction of ethyl acetate extract was chromatographed over macroporous adsorption resin (Diaion HP-20) eluted with a mixture of H2O and MeOH in increasing MeOH content.Their fractions from resin were repeatedly chromatographed over Toyopearl HW-40, Sephadex LH-20 and silica gel column chromatography. The compounds were identified on the basis of their physiochemical and spectral data. Results Four compounds were obtained and identified as 3,5-dihydroxy-stilbene-3-O-neohesperidoside ( 1 ), 3,5-dihydroxy-stilbene-3-O-β-D-glucoside ( 2 ), polydotin peceid (3) and 3,5,4'-trihydroxy-bibenzyl-3-O-β-D-glucoside (4). Conclusion Compound 1 is a new compound, the others were isolated from Dryopteris for the first time.

Aim To study the chemical constituents of Dryopteris sublaeta Ching et Hsu. Methods Fresh plant of Dryopteris sublaeta Ching et Hsu was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 ℃. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol. The fraction of ether extract was chromatographed over silica gel column. The compounds were identified on the basis of their physiochemical and spectral data. Results Four compounds were obtained and identified as 2 (S)-5,7, 3'-trihydroxy-6,8-dimethy1-5'-methoxyflavanone ( 1 ), matteucinol ( 2 ), desmethoxymatteucinol ( 3 ) and 5,7,2'-trihydroxy-6,8-dimethy1-flavanone (4). Conclusion Compound 1 is a new one, the others were isolated from Dryopteris for the first time.

Objective To optimize the extraction technology for total flavonoids in Selaginella tamariscina.Methods To determine the content of total flavonoids by UV and the content of index constituent of amentoflavone by HPLC.The optimum extraction condition was investigated by orthogonal design and the extraction quantity was regarded as the investigated index.Results The optimum extracting condition was A1B2C2D2 with the extraction quantity of total flavonoids as the investigated index.The optimum extracting condition was A1～3B1～3C2D2 with the extraction quantity of amentoflavone as the investigated index.The optimum extracting condition was A1B2C2D2.That is adding ten times amount of 95% alcohol and refluxing twice,2 h once.Conclusion The optimum technology is stable and feasible for the extraction of S.tamariscina.

Objective To optimize the extraction process f or polysaccharide in the residue of Fructus Jujubae(FJ ).Methods The conditions of extraction process for polysaccharide of FJ was screen ed with the yield and content of polysaccharides as markers by orthogonal experimental design.Results The optimum extraction process was:soaking the residue of FJ with water at 90℃for3times(5hours each time )and precipitated with 80%ethanol.T he yield and content of polysaccharides were 20%and 43.54%respectively.Conclusion This procedure is reliable and polysac-charides can be extracted completely by this procedure.It can bring a hig h economy benefit.