OBJECTIVE:To establish a method for the limit detection of aconitine in Wuqi shujin tongluo tablet. METHODS:TLC was conducted to identify the aconitine;TLC plate was silica gel G plate,developing solvent was toluene-ethyl acetate-chloro-form-acetone-ammonia(20:18:3:6:1,V/V/V/V/V),chromogenic agent was bismuth potassium iodide test solution and sodium ni-trite ethanol test solution;and durability investigation and detection limit detection were used to optimize the TLC plate,tempera-ture and humidity. RESULTS:TLC showed the aconitine had clear spots and negative control without interference. The durability was good;detection limit was 0.9 μg;available TLC plate was Merck HPTLC prefabricated plate, silica gel G TLC plate setf-made silica gel G TLC plate with adhesive of sodium carboxymethycellulose;temperature was 5-16 ℃ and humidity was 32%-72%. CONCLUSIONS:The method is simple and reproducibility,and can be used for the limit detection of aconitine in Wuqi shujin tongluo tablet.

Objective:To collect the bioburden information in clean room to understand the bioburden status, find out weak points and risks in microbial control and improve the management efficiency in clean room. Methods: According to GB/T 16293-2010 and the standard operation practice ( SOP) in our lab, the bioburden information was obtained by the collection and identification of air-borne microbe and surface bacteria in the four main areas of clean room ( microbial limit test room, sterile room 1 and 2, positive room) and on the person entered clean room. Results:The preliminarily established bioburden information indicated that the main mi-croorganism in clean room was Micrococcus and Staphylococcus. The detection rate of fungi was about 5% in clean room. Conclusion:The movement of people and goods in clean room should be strengthened, and samples should be with thorough disinfection.

Drug addiction is a chronic relapsing brain disease that is characterized by compulsive drug use and persistence of drug craving. Drug abuse can lead to changes in the neuron structure and function of plasticity,alterations in molecules and cells,and ultimately to individual abnormal behavior. Current studies have found that epigenetic changes leading to the sustainability of gene expression is an important mechanism of drug addiction. In this review,we will systematically summarize the latest advances in epigenetic mechanisms of drug addiction. This review is expected to provide robust evidence that repeated exposure to drugs of abuse induces changes within the brain′s reward regions in three major modes of epigenetic regulation-histone modifications such as acetylation and methylation , DNA methylation,and non-coding RNAs. It promises a new perspective from which to gain insights into the genetic and epigenetic mechanisms of drug addiction and a new area for epigenetic research on clinical drug addiction treatment.

BACKGROUND:Except for mechanical properties and physical and chemical properties, corrosion resistance testing is necessary for a variety of biomedical materials applied in the oral environment to ensure the biocompatibility of materials. OBJECTIVE:To explore the corrosion resistance of titanium abutment and different titanium aloysin vitro. METHODS:Corrosion environment byin vitroartificial saliva was constructed with pH=6.0 at (37±0.5)℃. Potentiodynamic polarization technique, scanning electron microscope and X-ray diffraction methods were employed to assess the corrosion resistance of titanium aloy, gold aloy, nickel-chromium aloy, and titanium abutment immersed in the artificial saliva for 24 hours. RESULTS AND CONCLUSION:Different aloys had different steady-state potentials, and the range of passivation region was the largest for gold aloy, folowed by titanium abutments and titanium aloys, and the smalest for nickel-chromium aloy. After 24 hours of immersion in the artificial saliva, passivation films appeared on the surface of different materials. under the scanning electron microscope, the nickel-chromium aloy surface showed obvious traces of corrosion and there were a large number of large-diameter deep pits, but no corrosion occurred on the surface of the remaining three kinds of aloys; on the surface of nickel-chromium aloy, the contents of chromium, molybdenum and aluminum were decreased, and the contents of nickel and oxygen were increased, but there were stil no changes on the surface of the remaining aloys. Cr2O3 was found on the surfaceof nickel-chromium aloy, TiO2 was generated on the surface of titanium abutment and titanium aloy, but Au and Pt stil existed in a single phase on the surface of gold aloy. These findings indicate that titanium aloy and titanium abutment have similar corrosion resistance that is inferior to the gold aloy, but better than the nickel-chromium aloy.

Objective To study the chemical constituents from Selaginella sinensis.Methods The compounds were isolated with Diaion HP-20,Toyopearl HW-40,silica gel column chromatography.The structures of these compounds were identified by physicochemical properties and spectral analyses.Results Eleven compounds were isolated from the 70% acetone-extracts and their structures were identified as ?-sitosterol(Ⅰ),vanillic acid(Ⅱ),(7S,8R)-4,9,9′-trihydroxy-3,3′-dimethoxy-7,8-dihydrobenzofuran-1′-propylneolignan(Ⅲ),syringaresinol(Ⅳ),(-)-pinoresinol(Ⅴ),pinoresinol-4-O?-D-glucopyranoside(Ⅵ),syringaresinol-4,4′-O-di-?-D-glucopyranoside(Ⅶ),?-methylD-xylopyranoside(Ⅷ),?-methyl-D-arabinopyranoside(Ⅸ),hinokiflavone(Ⅹ),and amentoflavone(Ⅺ).Conclusion Compounds Ⅱ-Ⅸ are isolated from this plant for the first time.

Objective To compare the efficacy of transumbilical-ultrafine gastroscope sympathectomy for severe palmar hyperhidrosis using two distinct levels of T3 and T4.Methods A total of 84 cases with severe primary hyperhidrosis were randomly allocated to undergo either T3 sympathectomy treatment (T3 group, n =42) or T4 sympathectomy treatment (T4 group, n =42) with no difference between the two groups.The operation time, postoperative hospital stay, patient's hands hyperhidrosis, axillary hyperhidrosis,complications were recorded at follow-up in 1,3,6,12 months.Results Operation on 84 patients were successful with mean operative time of T3 group being 55.02 ± 10.61 min and T4 group being 55.36 ± 10.51 min(P ＞ 0.05).The mean postoperative hospital stay were both 1.5 days.Patients were followed up for diaphragmatic hernia, umbilical hernia, Horner's syndrome and other serious complications for 12 months.No postoperative recurrence of palmar hyperhidrosis, severe compensatory sweating occurred in either group.The number of improved patients in T3 group's palmar hyperhidrosis,axillary hyperhidrosis, and foot hyperhidrosis were :42/42 cases, 10/16 cases, 21/29 cases, while those in T4 group were: 42/42 cases,16/17 cases 18/28 cases.Axillary hyperhidrosis improved to a larger extent in T4 group than in T3 group (P ＜0.05).There were 16(15 mild and 1 moderate)compensatory sweating in T3 group and 7(6 mild and 1 moderate) compensatory sweating in T4 group (P ＜ 0.05).Conclusion T3 and T4 thoracic sympathectomy using transumbilical-ultrafine gastroscope for primary palmar hyperhidrosis are safe, effective, and feasible.T4 sympathectomy is more effective in improving axillary hyperhidrosis than T3 and shows lower occurrence of postoperative compensatory hyperhidrosis.

Objective To investigate the effect of pre- hospital emergency care programs in children with febrile seizures,in order to reduce damage by febrile seizures and obtain the best firstaid effect. Methods 112 cases of children with febrile seizures were treated in pediatric department of our hospital from April 2009 to February 2011.The parents of 75 children patients were given guidance of prehospital emergency care,they were set as the pre-hospital emergency care group.The other 37 cases were set as the non-pre-hospital emergency care group,routine emergency care was given until the medical personnel arrived at the spot.The conditions of aspiration,tongue bite injury,another episode of febrile seizures and brain damage were recorded in two groups of children. Results Only one case of aspiration and three cases of febrile seizures occurred in the pre-hospital emergency care group by misoperation of parents.Non brain damage and tongue bite injury occurred.The incidence rate of improper consequences was 5.3％in the pre-hospital emergency care group,lower than that of the non-pre-hospital emergency care group,51.4％. Conclusions To give pre-hospital emergency care guidance to parents of children patients by mobile phones before the health care workers reach the spot can significantly reduce accidental injury of children,stabilize the emotion of parents,decrease the impact of seizures on children,all the above has a positive effect on follow-up treatment.

Objective To reduce the rate of dysphagia and inhalation pneumonia by changing the food dense and evaluate the swallow function by videofluoroscopy(VF) and fiberoptic endoscopic examination of swallowing(FEES). Methods The videofluoroscopy and fiberoptic endoscopic examination of swallowing were used in 50 healthy volunteer and 35 dysphagia person. Results Aspiration was find in 14% volunteer and penetration was find in 36% volunteer. The positive rate of videofluoroscopy was higher than of fiberoptic endoscopic examination of swallowing in the two indicators. The delay of the thin barium passed the pharynx was the danger factor of aspiration. Videofluoroscopy was more sensitive in diagnosis of penetration. The time that watery barium and pudding barium flowed from pharynx to epiqlottis is longer in patients than that in volunteer. Conclusion The videofluoroscopy and fiberoptic endoscopic examination could be predicted to some extend by some clinical swallowing abnormalities, which could increase the accuracy of clinical evaluation . The viscosity changing can decrease the rate of dysphagia and inhalation pneumonia.

Objective To investigate the current infection situation of Chlamydia trachomatis (CT) and Ureaplasma urealyticum (Uu) in patients with cervical erosion .Methods CT and Uu were detected in cervical secretions of 562 cases of cervical erosion pa‐tients (observation group) and 218 healthy controls (control group) .Drug susceptibility testing was performed for the patients with Uu infection .Results Positive rate of CT ,Uu and mixed infection was 20 .11% ,42 .35% and 9 .61% ,higher than those of control group (P<0 .05) .The difference of the positive rate between moderate cervical erosion and severe cervical erosion was not signifi‐cant (P>0 .05) ,while the positive rate of CT ,Uu and mixed infection was significantly different between the two groups and mild cervical erosion (P<0 .05) .The highest susceptibility rate of Uu to antimicrobial agents was josamycin (91 .78% ) ,followed by pristinamycin (84 .25% ) .The highest resistant rate of Uu was to ciprofloxacin (85 .96% ) ,followed by ofloxacin(73 .29% ) .Conclu‐sion The infection of CT and Uu could be intimate correlated with cervical erosion and the extent of erosion .Varying degrees of drug resistance of Uu could be found ,and the sensitive and effective antibiotics should be chosen to treat cervical erosion infection of Uu .

Objective To explore the relationship between the damage of neuromyelitis optica (NMO) astrocytes (AS) and the onset of NMO, and investigate the relevance of AS damage with the severity of the patients with functional defect. Methods The levels of aquaprin4-antibody (AQP4 -Ab), glial fibrillary acidic protein (GFAP), apolipoprotein(ApoE),interleukins-6(IL-6), interleukins-10(IL-10), tumor necrosis factor-α(TNF-α) in cerebrospinal fluid (CSF ) and serum of 30 acute NMO patients were tested by means of ELISA. The results were later compared with control group. And analysis of the relevance of the various index of the levels in CSF with the CSF AQP4-Ab level, the acute phase expanded disability status scale(EDSS) score of the NMO group were made. Results (1)The NMO group in CSF and serum AQP4-Ab, GFAP, IL-6 levels were higher than the control group (P < 0.05), and ApoE, IL-10 levels were lower than the control group (P < 0.05). (2)The CSF GFAP, ApoE, IL-6 in NMO group is higher than the serum (P < 0.05), and CSF AQP4-Ab, IL-10 levels were lower than the serum ( P < 0 . 05 ) . ( 3 ) The CSF GFAP , IL-6 levels and the CSF AQP4-Ab level were positively correlated (r=0.749, r=0.526, P<0.05), and the CSF ApoE, IL-10 levels were negatively correlated with CSF AQP4-Ab level(r = -0.571, r = -0.676, P < 0.05). (4)The CSF AQP4-Ab, GFAP, IL-6 levels and the acute phase EDSS score were positively correlated (P < 0.05), the CSF ApoE, IL-10 levels were negatively correlated with the acute phase EDSS score (P < 0.05). Conclusion The AS damage exists in the NMO and the damage severity may correlate with patient function defect. AQP4-Ab, GFAP, IL-6 may play important roles in the onset of NMO and the disease aggravating. The decrease of the ApoE and IL-10 may exacerbate NMO damage.