1.Immunologic Classification of Acute Lymphocytic Leukemia: A Report of 19 Cases
Fang YAN ; Yanqun XU ; Fenglai HAN ; Weirong WEN
Academic Journal of Second Military Medical University 1982;0(02):-
Immunologic classification of acute lymphocytic leukemia (ALL) was carried out by using 11 - 13 kinds of monoclonal antibodies against human leucocyte differentiation antigens in 19 cases. The results showed that of these 19 cases, there were T-ALL in 4, B-ALL in 4, C-ALL in 7, AUL in 2 and hybrid type of ALL (H-ALL) in 2. The 5 types mentioned above could also be classified, according to the immunologic criteria described by Foon in 1986, into 3 major groups: T-ALL, non-T-ALL and H-ALL. The Foon's classification method is useful for judgement of malignant cell source and differentiated stage, and for further understanding the nature of ALL cells.
2.Effects of HZ08,a novel P-glycoprotein inhibitor, on the reversal of P-glyco-protein mediated multidrug resistance in nude mice and cytochrome P-450 ac-tivities in rat liver microsomes
Fang YAN ; Yunman LI ; Qiujuan WANG ; Weirong FANG ; Kai KANG ; Luyong ZHANG
Journal of China Pharmaceutical University 2008;(5):447-452
Aim: To evaluate the effects of HZ08, a novel P-glycoprotein inhibitor, on reversing tumor resistance of K562/ADM to adriamycin in nude mice and on the activities of cytochromes P-450 (GYP) isoforms. Methods: Nude mice bearing K562/ADM were injected at different doses of HZ08 with adriamycin for 4 weeks. The tumor weights of HZ08 treatment groups were determined and compared to those of the control and positive groups. In addition, the effects of HZ08 were examined on GYP isoforms-mediated metabolism of specific substrates by GYP isoforms in rat liver microsomes in the presence or absence of HZ08. Results: The tumor weights of HZ08 treatment groups were significantly decreased and HZ08 was a relatively potent inhibitor of CYP3A4, with no significant effects on other isoforms tested. Conclusion: HZ08 has potent effects on reversing P-glycoprotein mediated tumor multidrug resistance in rive with little influence on cytoehrome P-450 activities of rat liver.
3.Therapeutic time window of dimethylaminoethyl ginkgolide B mesylate in permanent focal ischemia of rat
Yan QI ; Li BAI ; Peng Lü ; Weirong FANG ; Yunman LI ; Lishun MAO
Journal of China Pharmaceutical University 2010;41(2):166-170
In order to study the therapeutic time window of dimethylaminoethyl ginkgolide B mesylate(XQ-1H) in the permanent focal ischemia of rat,we used the rat model of the permanent middle cerebral artery occlusion (pMCAO).Doses of 15.6,7.8 and 3.9 mg/kg of XQ-1 H were intravenously administered at 0.5,1,2,3 h after MCAO,respectively.Neurological scores,infarct sizes,water contents and pathological changes in each interval were determined at 72 h after MCAO.It was observed that XQ-1 H administered at 0.5 and 1 h after MCAO significantly reduced the cerebral infarct size and edema,and produced significant reductions in the neurological deficits.The protective effect of XQ-1H on the neuron cells was proved by pathological observations.In addition,the contents of MDA,lactate,and the activities of SOD were measured.Reduction in the contents of MDA and lactate and enhancement in the activities of SOD were attributed to the pretreatment of XQ-1H at 0.5 and 1 h.Our results showed that the therapeutic time window of XQ-1H extended for up to 1 h after MCAO.
4.Prenatal diagnostics and outcome of ultrasonographic soft markers
Weirong GU ; Xiaotian LI ; Yingliu YAN ; Yueping ZHANG ; Mingwei ZHU ; Juehua ZHANG ; Yuqing ZHOU
Fudan University Journal of Medical Sciences 2010;37(2):180-183,188
Objective To review the clinical treatment and outcome of ultrasonographic soft markers in prenatal diagnostics. Methods This study recruited 268 pregnant women who underwent prenatal diagnostics in our hospital between Jun 2005 to Mar 2009. Fetuses were followed up postnatally. The outcome and chromosomal abnormalities of ultrasonographic soft markers were assessed. Results Of 268 cases consulted, 29 cases were missed (10.8%), 34 cases (12.7%) chose abortion, and 205 cases (76.5%) delivered. The top four most common delivered isolated markers were thickened nuchal fold, mild pyelectasis, echogenic bowel and rhizomelic limb shortening. Mild ventriculomegals had the highest aborted rate (17.2%). Six chromosomal structural abnormalities and one 21-trisome were detected in 59 fetuses who received chromosomal examination. Conclusions Ultrasonographic soft markers are risks to both fetal trisome and chromosomal structural abnormalities. Owing to extinction in most cases, consultant should be strengthed to avoid unnecessary invasive examination and abortion.
5. Association between both maternal and fetal angiotensinogen gene single nucleotide polymorphism and preeclampsia/eclampsia
Shaojing YU ; Weijun PENG ; Heng ZHANG ; Xianzhen CHEN ; Muhong WEI ; Weirong YAN
Chinese Journal of Epidemiology 2019;40(8):997-1002
Objective:
To explore the association between preeclampsia/eclampsia and maternal and fetal angiotensinogen SNPs.
Methods:
From January 2008 to October 2015, a case-parents/mother-control designed study was conducted among 347 preeclampsia/eclampsia cases and 700 controls to collect related information on their demographic characteristics and to detect the related angiotensinogen SNPs’ genotypes. Both log-linear and unconditional logistic regression methods were employed to investigate the genetic effects of maternal/fetal angiotensinogen SNPs on preeclampsia/eclampsia. Multivariate binary unconditional logistic regression model and covariance were used to analyze the relationship between BMI before pregnancy, weight gain during pregnancy and overweight and obesity in preschool children.
Results:
Both fetal angiotensinogen rs3789679 GA and AA genotype were associated with the reduced risks of preeclampsia/eclampsia, with
6.Protective effect of Ganoderma lucidum spore glycopeptide on liver injury in mice
Peng FENG ; Xuan FEI ; Yan CHEN ; Qinglong GUO ; Weirong FANG
Journal of China Pharmaceutical University 2021;52(5):603-608
Models of acute and chronic liver injury in mice were established using carbon tetrachloride (CCl4) and ethanol to explore the protective effects of Ganoderma lucidum spore glycopeptide on liver injury.Different dosage of Ganoderma lucidum spore glycopeptide (65,130,260 mg/kg) were given by gavage.The liver index and the levels of serum aspartate transaminase (AST) and alanine transaminase (ALT) were determined.The contents of liver interleukin-6 (IL-6), tumor necrosis factor (TNF-α) and inducible nitric oxide synthase (iNOS) were tested by enzyme-linked immunosorbent assay (ELISA).The pathological injury of liver tissue was observed by HE staining.The results showed that Ganoderma lucidum spore glycopeptide could significantly reduce the liver index and the contents of serum AST and ALT in mice of acute and chronic liver injury.In mice of chronic liver injury induced by CCl4, Ganoderma lucidum spore glycopeptide could significantly decrease the contents of liver IL-6, TNF-α and iNOS, and alleviate the pathological damage of liver tissue.Results suggested that Ganoderma lucidum spore glycopeptide might reduce acute and chronic liver injury with anti-inflammatory effects in mice.
7.Expression of betaig-h3 in keratoconus and normal cornea.
Guiqiu ZHAO ; Chuanfu WANG ; Weirong SUN ; Wenqing ZHANG ; Yan LI ; Hui SHENG ; Tao LIANG
Chinese Medical Journal 2002;115(9):1401-1404
OBJECTIVESTo observe the expression of betaig-h3 in normal cornea and keratoconus and to elucidate the role of extracellular matrix in keratoconus.
METHODSIn situ hybridization was used to detect the expression of betaig-h3 in the cornea. The cDNA library was screened with human betaig-h3 cDNA probe to locate betaig-h3 mRNA in cells.
RESULTSExpression of betaig-h3 was found mainly in the stroma of the normal cornea and keratoconus, but decrease depending on the degree of keratopathy. In some serious cases, no expression signal was detected. The strongest expression was seen at the border of the normal region and keratoconus.
CONCLUSIONSbetaig-h3, the structural component of the extracellular matrix, can affect cell adhensiveness in the development of corneal fibrous interstitial organization. During the development of keratoconus, decreasing levels of betaig-h3 cause the diminution of corneal steadiness, which is related to formation of keratoconus.
Cornea ; metabolism ; Extracellular Matrix Proteins ; Humans ; Keratoconus ; metabolism ; Neoplasm Proteins ; genetics ; RNA, Messenger ; analysis ; Transforming Growth Factor beta ; Wound Healing
8.Single nueleotide polymorphism array detection of Xq28 duplication in a child with mental retardation.
Yan WANG ; Jingjing ZHANG ; Ling QIN ; Lulu MENG ; Tao JIANG ; Dingyuan MA ; Weirong HUI ; Ping HU ; Zhengfeng XU
Chinese Journal of Pediatrics 2014;52(3):227-228
Child
;
Chromosome Duplication
;
genetics
;
Craniofacial Abnormalities
;
diagnosis
;
genetics
;
Facies
;
Genetic Diseases, X-Linked
;
diagnosis
;
genetics
;
Humans
;
Intellectual Disability
;
diagnosis
;
genetics
;
Male
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Methyl-CpG-Binding Protein 2
;
genetics
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Oligonucleotide Array Sequence Analysis
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Polymorphism, Single Nucleotide
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Sex Chromosome Disorders
;
diagnosis
;
genetics
9.Study on Pulse Recordings of College Students with Different Emotional States
Huijiang SHAO ; Wei YANG ; Weirong PAN ; Rui GUO ; Yiqin WANG ; Haixia YAN
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(7):1214-1218
This paper was aimed to investigate the changes of pulses under different emotional states.Based on the emotional pulse theory of traditional Chinese medicine (TCM),with college students as the research object,through films to stimulate different emotional expressions (i.e.,calm,sad and happy) of college students,the pulse recordings of college students were acquired in this study.The time domain parameters of pulses of college students under three types of emotional states were extracted.The difference among three groups of time-domain parameters was calculated by nonparametric test.Based on the time-domain parameters,the Support Vector Machine (SVM) classifier was used to classify three types of emotional states.The results showed that there were significant differences in three groups of time domain parameters (h3/h1,t and As/(As+Ad)).And the average recognition rate of three types of emotion states was 74.25% based on the time-domain parameters of the pulse recordings.It was concluded that the pulse parameters can provide objective reference for emotional changes.It can be used to identify emotional states.
10.Array comparative genomic hybridization detection of a de novo 4q21.21-q22.1 deletion in a child with severe growth retardation.
Jing ZHOU ; Ping HU ; An LIU ; Li LI ; Xiuqing JI ; Weirong HUI ; Yan WANG ; Zhengfeng XU
Chinese Journal of Medical Genetics 2014;31(1):52-55
OBJECTIVETo analyze a child with developmental delay, severe mental retardation, speech delay and muscular hypotonia.
METHODSThe karotypes of the child and her parents were analyzed with G-banding analysis. Their genome DNA was also analyzed with array comparative genomic hybridization (array-CGH).
RESULTSNo karyotypic abnormality was detected at cytogenetic level. However, array-CGH has identified a de novo 4q21.21-q22.1 deletion in the child, which has a size of 12.1 Mb.
CONCLUSIONThe de novo interstitial 4q21.21-q22.1 deletion probably underlies the main clinical manifestation in the child. Array-CGH is useful for diagnosing children with multiple congenital anomalies with unclear etiology.
Child, Preschool ; Chromosomes, Human, Pair 4 ; Comparative Genomic Hybridization ; methods ; Female ; Gene Deletion ; Growth Disorders ; diagnosis ; genetics ; Humans ; Intellectual Disability ; diagnosis ; genetics ; Sequence Deletion