1.Correlation between Stress Reaction and other Stress Related Factors in Neurosis Patients
Weirong REN ; Qianjin JIANG ; Liju QIAN ; Zhixia WU
Chinese Journal of Clinical Psychology 2000;0(04):-
Objective: To investigate the correlation between stress reaction and other related psychological stress factors such as life events, social support, and coping style in neurosis patients comparing with the group of healthy persons. Methods: 88 neurosis and 100 healthy persons were examined with LES (Life Event Scale), PSSS (Perceived Social Support Scale), TCSQ (Trait Coping Style Questionnaire), SRQ(Stress Reaction Questionnaire). Results: The scores of stress reaction, family events and negative coping style of neurosis group were significantly higher than those of healthy group, and the scores of social support out of family and positive coping style were significantly lower than those of healthy group. The regression analysis and path analysis revealed that in neurosis group stress reaction was directly correlated with negative coping style and social events, but in healthy group it was correlated with negative coping style and family events. Conclusion: Stress reaction may be directly affected with negative coping style and social events in neurosis patients, whereas it may be done with negative coping style and family events in healthy persons.
2.COMPOUND MUTATION BREEDING OF KOJIC ACID PRODUCTION STAIN
Weirong SHEN ; Jian SHEN ; Liping HAN ; Ying JIANG ; Yi WAN ; Rui CHEN ;
Microbiology 1992;0(04):-
mutant (UCN 7 17) of producing high yield Kojic acid was screened fr om Aspergillus flavus after treated with UV three times, ? ray of 60 Co one time and NTG four times, underoptimal conditions, the Kojic aci d production level reached up to 6 3% after 7 days, compared with original stains 0 926% The experiments showed that compound mutation using various mutagenic agents ca n alter the original stains sensitivity to mutagenic agents, increase mutation frequency and raise Kojic acid yield
3.Risk factors for preoperative full stomach in patients undergoing emergency surgery
Weirong TAO ; Hong XIE ; Jiang ZHU ; Qiaofen JIANG
Chinese Journal of Anesthesiology 2020;40(8):926-928
Objective:To identify the risk factors for preoperative full stomach in the patients undergoing emergency surgery.Methods:American Society of Anesthesiologists physical status Ⅰ-Ⅲ patients of both sexes, aged≥18 yr, with body mass index ≥15 kg/m 2, scheduled for elective emergency surgery, were included.The operation bed was adjusted to 30° head-high and feet-low position.Ultrasound images of gastric antrum were observed in the right decubitus position, and the ultrasonic images were evaluated qualitatively and quantitatively.The ultrasonic image was qualitatively assessed as having empty antrum, liquid or solid.Three images were obtained during the diastolic period of gastric antrum when quantitative assessment was performed.The longest diameter (D1) and the widest diameter (D2) of the images were measured three times and averaged to calculate the cross-sectional area of gastric antrum [CSA=(D1×D2×π)/4]. The gastric volume was calculated [GV=27.0+ (14.6×CSA)-(1.28×age)]. When the result was<0, the gastric volume was 0.GV/body weight was calculated, and GV/body ≤1.5 ml/kg was considered as empty stomach.When gastric content was solid or GV/body weight>1.5 ml/kg, it was considered as full stomach.Patients were divided into 2 groups according to whether they presented with a full stomach: empty stomach group (group E) and full stomach group (group F). The patients′ fasting time, coexisting diseases and types of operation were recorded.Multivariate logistic regression analysis was performed on the indicators with statistically significant differences between groups to identify the risk factors for preoperative full stomach in the patients undergoing emergency surgery. Results:One hundred and seventy-nine cases were finally included, with 121 cases in group E and 58 cases in group F. Compared with group E, the fasting time was <6 h, and the incidence of coexisting diabetes was increased in group F ( P<0.05). Coexisting diabetes mellitus was an independent risk factor for preoperative full stomach in the patients undergoing emergency surgery ( P<0.05), and OR (95% confidence interval) was 11.968 (2.392-59.870). Conclusion:Coexisting diabetes mellitus is an independent risk factor for preoperative full stomach in the patients undergoing emergency surgery.
4.The effects of advanced oxidation protein products (AOPP) on reactive oxygen species (ROS) production in MC3T3-E1 cells by NADPH oxidase enzymes pathway
Aiju LOU ; Weirong WU ; Keren HE ; Zihong CAI ; Qiwang LIN ; Menglin HU ; Jianling MO ; Xiaoyan CAI ; Qixin XIE ; Chunmei JIANG ; Le WANG
Chinese Journal of Endocrinology and Metabolism 2016;32(5):405-410
Objective In the present study, we investigated the effects of advanced oxidation protein products(AOPP) on reactive oxygen species(ROS) production in murine osteoblastic MC3T3-E1 cells by NADPH oxidase enzymes pathway. Methods Experiments were divided into three groups, including control group, rats albumin(RSA) group, and AOPP group. Different concentrations of AOPP were added to the osteoblastic MC3T3-E1 cells culture medium. The production of ROS in MC3T3-E1 cells was measured by the fluorescence intensity of intracellular fluoroprobe ( DCFD ) . In order to verify the effect of enzyme of the production of ROS, the specific inhibitors of corresponding enzymes were added in the MC3T3-E1 cells which were cultured in the medium with AOPP. Finally, western blot and immunofluorescence were used to observe the changes of NADPH oxidase enzymes subunits. Results Different concentrations of AOPP (50,100,200μg/ml) induced MC3T3-E1 cells to produce different amount of ROS. The higher concentrations of AOPP were added, the more ROS were produced. Furthermore,200μg/ml AOPP induced the maximum amount of ROS production(P<0. 05). Meanwhile, AOPP induced MC3T3-E1 cells to produce different amount of ROS with a time-dependent manner. The peak amount of ROS production in MC3T3-E1 cells was observed in 3h when AOPP were added (P<0. 05). In addition, when specific inhibitors of corresponding enzymes were added in the MC3T3-E1 cells, the production of ROS were significantly suppressed by C-SOD, DPI, and apocynin(P<0. 05). On the other hand, AOPP can up-regulate the expression of Nox4 protein of the MC3T3-E1 cells, which is one of the subunits of NADPH oxidase enzymes. Meanwhile, AOPP can also induce the membrane migration of p47phox subunit. Conclusion AOPP induces osteoblastic MC3T3-E1 cells to produce ROS by NADPH oxidase enzymes pathway, and which may be one of the pathogenesis of AOPP involved in osteoporosis.
5.EVALUATION OF THE EFFECT OF INTERLEUKIN-2 ACTIVATED AUTOLOGOUS HUMAN PERIPHERAL BLOOD LYMPHOCYTES ON CHRONIC HEPATITIS B
Chengwei CHEN ; Yuanzhai LI ; Hanyang XUE ; Guangcai YANG ; Bingchen HUANG ; Gengsheng WANG ; Yuelan WANG ; Shisong JIANG ; Lianfang HU ; Weirong TAO ; Liuda NI ; Qingchun FU
Medical Journal of Chinese People's Liberation Army 1982;0(03):-
24 patients with chronic hepatitis B were treated with interleukin-2 activated human peripheral blood lymphocytes (IAPBL). 23 patients served as control. The results showed that HBeAg disappeared in 54.2% of IAPBL group, in comparison with 17.4% of the controls (P
6.Single nueleotide polymorphism array detection of Xq28 duplication in a child with mental retardation.
Yan WANG ; Jingjing ZHANG ; Ling QIN ; Lulu MENG ; Tao JIANG ; Dingyuan MA ; Weirong HUI ; Ping HU ; Zhengfeng XU
Chinese Journal of Pediatrics 2014;52(3):227-228
Child
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Chromosome Duplication
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genetics
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Craniofacial Abnormalities
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diagnosis
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genetics
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Facies
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Genetic Diseases, X-Linked
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diagnosis
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genetics
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Humans
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Intellectual Disability
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diagnosis
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genetics
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Male
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Methyl-CpG-Binding Protein 2
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genetics
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Oligonucleotide Array Sequence Analysis
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Polymorphism, Single Nucleotide
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Sex Chromosome Disorders
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diagnosis
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genetics
7.Progress in the diagnosis and treatment of chronic postoperative inguinal pain
Binjie SUN ; Yunfeng LI ; Kun YU ; Weirong JIANG ; Yongjiang YU
International Journal of Surgery 2021;48(7):493-499
Inguinal hernia is one of the most common diseases in general surgery. Surgery is the only treatment. In recent years, with the emergence and popularization of tension-free hernia repair, the recurrence rate has been lower than before. Chronic Postoperative Inguinal Pain (CPIP) has gradually become the focus of research. CPIP has now become one of the important efficacy indicators for inguinal hernia surgery. The etiology of CPIP is more complicated, mainly including neuropathic pain, non-neuropathic pain, somatic pain and visceral pain. Female, young, obese, low pain control, preoperative anxiety, preoperative pain, high pain sensitivity and other patient factors, and experience of the surgeon, open hernia repair, weight patch, patch fixation, surgery Surgical factors such as post-acute pain are risk factors for CPIP. CPIP is not only a product of neuropathic and nociceptive pain, but is also affected by various factors such as psychology, emotion, cognition, and genetics. Therefore, detailed medical history, physical examination, and correct pain and quality of life assessment tools are essential for the diagnosis of CPIP is very necessary. The treatment of CPIP should follow certain steps. The first choice is anticipatory treatment, drug treatment, psychological and behavioral treatment, physical therapy and other conservative treatments and interventional treatments, If the pain relief is not obvious after 6 months to 1 year by the above methods, surgical treatment is considered. So far, preventive analgesia and standardized surgery are the most important means to improve the prognosis of patients.
8.Advance in abdominal hernia repair based on enhanced recovery after surgery
Yunfeng LI ; Yongjiang YU ; Binjie SUN ; Weirong JIANG ; Kun YU
International Journal of Surgery 2021;48(10):695-699
Enhanced recovery after surgery (ERAS) is a systematic approach to care that optimizes perioperative management, improves clinical outcomes, and reduces healthcare costs. ERAS has been introduced into abdominal hernia repair in recent years. Preoperative management was optimized through health education, smoking cessation, weight control, blood glucose and pain management, infection and thrombosis prophylaxis. And prevention of hypothermia, standardized anesthesia management, fluid replacement control, minimally invasive operation, controlled catheter placement and other intraoperative management optimization measures. And multi-mode analgesia, early postoperative activity, recovery of gastrointestinal function and control of blood glucose and other postoperative management optimization measures effectively reduced the incidence of complications such as surgical site infection, alleviated the pain of patients, and shortened the hospital stay. ERAS factors such as perioperative pain management, early postoperative activity, and recovery of gastrointestinal function are the key to shorten hospital stay and improve prognosis during abdominal wall hernia repair. ERAS can be used safely and effectively for abdominal hernia repair.
9. Change in peripheral nervous conduction velocity in patients with occupational chronic mercury poisoning and related influencing factors
Li LI ; Bo JIANG ; Juan LAI ; Weirong DAI ; Xin LI ; Wenfeng LIU ; Zhongxing YANG ; Lei XIE
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(8):598-602
Objective:
To investigate the change in peripheral nervous conduction velocity in patients with occupational chronic mercury poisoning and related influencing factors.
Methods:
From February 2011 to December 2014, urinary mercury examination and neural electromyography were performed for 104 patients with occupational chronic mercury poisoning. The data on age, type of work, working years of mercury exposure, and past medical history were collected, and peripheral nervous conduction velocity and its correlation with age, working years of mercury exposure, and urinary mercury concentration were analyzed.
Results:
All the 104 patients with occupational chronic mercury poisoning had a mean of 45.37±9.82 years, median (
10.Research progress in STAT3/Th17 cells and Sj?gren syndrome
Jiang WANG ; Xueyan ZHAO ; Weirong FANG
Journal of China Pharmaceutical University 2024;55(3):420-428
Signal transducer and activator of transcription 3(STAT3)is an intracellular signaling factor that plays a critical role in various cellular processes,including the growth,differentiation,apoptosis,and immune response of cells.Aberrant activation of T helper cell 17(Th17)is closely associated with the morbidity and progress of various autoimmune diseases.STAT3 participates in the pathogenesis of Sj?gren syndrome by inducing excessive proliferation and abnormal differentiation of Th17 cells and affecting lymphocyte infiltration into exocrine glands.Therefore,targeting the STAT3 signaling pathway represents a potential novel therapeutic approach for the treatment of Sj?gren syndrome.This review summarizes the research of STAT3 in the pathogenesis and progression of Sj?gren syndrome through regulating Th17 cells,focusing on current inhibitors targeting the STAT3 signaling pathway as potential therapeutic targets for Sj?gren syndrome.