Objective To investigate clinical effects of laparoscopic salpingotomy combined with pituitrin in the treatment of tubal pregnancy.Methods 160 children with tubal pregnancy were chosen in our hospital in the period and randomly divided into two groups including control group (80 children)with laparoscopic salpingotomy treatment and treatment group (80 children)with pituitrin adjunct treatment before operation on the basis of control group.And the operation time,intraoperative bleeding volume,postoperative body temperature,hospitalization time,the retention rate of fallopian tube,fallopian tube patency rate,persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of both groups were compared.Results The operation time,intraoperative bleeding volume, postoperative body temperature and hospitalization time of the control group were separately (20.33 ±2.70)min, (52.76 ±7.42)mL,(37.52 ±0.54)℃ and (7.56 ±1.98)d;the operation time,intraoperative bleeding volume, postoperative body temperature and hospitalization time of the treatment group were separately (8.94 ±1.61)min, (16.33 ±2.15)mL,(37.46 ±0.50)℃and (5.91 ±1.42)d;the operation time,intraoperative bleeding volume and hospitalization time of the treatment group were significantly better than those of the control group (t=2.26,2.60, 2.08,all P<0.05 );there was no significant difference in postoperative body temperature between 2 groups (t=1.55,P>0.05).The retention rate of fallopian tube and fallopian tube patency rate of the control group were sepa-rately 75.00% (60/80)and 52.50%(42/80);the retention rate of fallopian tube and fallopian tube patency rate of the control group were separately 93.75% (75/80)and 81.25% (65/80);the retention rate of fallopian tube and fallopian tube patency rate of treatment group were significantly better than those of the control group (χ2 =9.71, 10.14,all P<0.05).The persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of the control group were separately 17.50%(14/80),31.25%(25/80)and 37.50%(30/80);the persistent ectopic pregnancy rate,intrauterine pregnancy rate and fertility rate of the treatment group were separately 15.00% (12/80),61.25%(49/80)and 16.25% (13/80);there was no significant difference in persistent ectopic pregnancy rate between 2 groups (χ2 =1.33,P>0.05);the intrauterine pregnancy rate and fertility rate of treatment group were significant-ly better than those of the control group (χ2 =11.84,12.52,all P<0.05).Conclusion Laparoscopic salpingotomy combined with pituitrin in the treatment of tubal pregnancy can efficiently decrease operation time the amount of bleeding,shorten hospitalization time and be helpful to improve the reproductive outcome.

Bronchopulmonary dysplasia remains the most common severe complication of preterm in-fants,affecting the quality of life in preterm children seriously. There is no specific or effective treatment availa-ble to date for BPD. Since the aetiopathogenesis of BPD is multifactorial,involving diverse molecular signaling pathways,a variety of biomarkers detected in biological fluids have been proposed for early identification of in-fants predisposed to BPD.

Objective To learn how well these items of Hubei Province meet the quality standards of allowed imprecision. Methods Collected the indoor quality control data of median concentration levels from the laboratories which participated the project of interlaboratory comparisons of clinical chemistry indoor quality control data in Hubei Province.This paper was to analyze the variation coefficient of indoor quality control for 21 routine clinical chemistry examination items which were K,Na,Cl,TCa,P,GLu,Urea,UA,Cr,TP,Alb,TC,TG,ALT,AST,TBil,ALP,AMS,CK,LDH andγ-GT.The other objec-tive was to learn how well these items of Hubei Province meet the quality standards of allowed imprecision.Then took the 1/3 TEa,1/4 TEa,WS/T-403-2012 and minimum imprecision derived from biological variation as quality specification.And an-alyzed the percentage of laboratories in meeting the quality standards.Results The TG,ALT,CK and TBil in more than 50% of the participated laboratories could meet the quality standards of the 1/3 TEa,1/4 TEa,WS/T-403-2012 and the low-est appropriate imprecision derived from biological variation.The Cl and Cr in more than 50% of laboratories couldn’t meet the all above quality standards.The Na and TCa in all laboratories couldn’t meet the quality standards of best imprecision derived from biological variation.The evaluation criterion for qualified items setted was that the variation coefficient in more than 80% laboratories was less than the quality standard.Thus,the percentage of the items which meet the lowest quality standard of biological variation and the all 21 items was the most (66.7%).While the percentage of the items which met the quality standards of the WS/T403-2012 and the best biological variation was the least (14.3%).Conclusion In short,the values of indoor variation coefficient of the 21 items in laboratories which participated the project of interlaboratory compari-sons of clinical chemistry indoor quality control data generally met the requirements.But some items had a little higher de-gree of dispersion.The laboratories should set the appropriate imprecision levels based on the detection capability and quality standards and improve the quality of examination through continuous efforts.

Objective To explore the causes, management and outcome of pregnant women with thrombocytopenia. Methods Medical records from 1999 to 2003 were reviewed for diagnosis,treatment ,and neonatal outcome in 130 women with thrombocytopenia in Xinhua Hospital. Results Thrombocytopenia was mainly caused by prenancy-associated thrombocytopenia (PAT) in 94 (72 3%) cases,idiopathic thrombocytopenia(ITP) in 12 cases (9 2%),hepatic disease in pregnancy in 11 (8 5%)cases, pregnancy induced hypertension 10 cases and preeclampsia 4 (10 8%)cases, Rhesus isoimmunization and viral infection each 1(0 8%) case. Thrombocytopenia was identified initially at 17 +4 weeks of gestation,37 (28 5%) cases were found before 28 weeks,93 (71 5%) cases were found after 28 weeks. Prednisone was considered if the platelet count was less than 50,000/uL. Platelet transfusion was given to those with platelet count less than 50,000/uL before and after delivery. If the platelet count was less than 50,000/uL esarean delivery might be performed. The occurrence of postpartum hemorrhage was 2 3%. Bleeding occurred in two neonatals. One newborn suffered from cephalohematoma. Sixty-three cases PAT recovered spontaneousely within 1 week after delivery. Conclusion Pregnancy thrombocytopenia usually becomes evident in the third trimester. PAT is the most common type of thrombocytopenia during pregnancy. Pregnancy thrombocytopenia is not associated with bleeding diathesis in the mother. Degree of thrombocytopenia with complication was more severe(platelet count usually less than 70,000/uL). Half of ITP are symptomatic. Glucocorticoid is effective treatment for severe thrombocytopenia. Platelet transfusion may be considered when platelet count is still less than 50,000/uL before operation. Mother with ITP may affect infant.Besides therapy directing at the eiology, corticosteroids and platelet transfusion are effective treatment for severe thrombocytopenia during delivery. One half women return to a normal platelet count within one week after delivery.

Objective To investigate the efficacy and safety of Topiramate as adjunctive therapy in patients with refractory epilepsy.Methods 115 patients enrolled in this open-label,self-controlled prospective study which were designed to adopt slower dosage escalation schedule with lower initial,until reached target dosages(up to 8 weeks) or the most tolerable dosages of Topiramate,and maintained them 1~3 years treatment.The long-term efficacy,safety of Topiramate and their relationship with dosage were evaluated.Results The median percentages reduction were 56.0%,75.8%,76.1%,77.3% and 78.1% after 8 weeks,6 months,1 year,2 years and 3 years Topiramate treatment respectively for adults,and 32.1%,66.7%,68.9%,70.1% and 70.8% respectively for children.The overall efficacy for partial seizures(70.6%) was higher than that for generalized seizures(37.5%)((P

Objective To observe the effect of synthetic corticotropin with vitamin B 6 on intractable epilepsy of children.Methods 20 patients (3 months to 4 years old) among which 9 children with infantile spasms and 11 children with intractable epilepsy were injected with vitamin B 6 (50～100 mg/d) for 10 days,then treated with cortrosyn 0 015~0 025 mg/Kg?d by the interval mothod of lengthening dosage,total course of treatment was about 2 months,seizure,EEG and some blood biochemical tests were observed in all patients before and after the course of treatment.Results The seizure of 14 patient children stopped after the treatment,seizure frequency was decreased in 5 patients (from 15 to 40 times/week decreasing to 3 to 14 times/week respectively),1 patient with intractable epilepsy was stopped treating because of side effect.The general clinical condition and EEG were also improved.The blood biochemical tests about liver and kidney were normal during treatment,hypokaliemia,hypocalcemia and slight dropsy were appeared in some patients.Conclusion Cortrosyn with vitamin B 6 is effective for infantile spasms and intractable epilepsy in children of low age.

Objective To study the effects of adenovirue-mediated p27kipl gene and its protein product overexpression on neointimal proliferation of rabbit carotid artery after balloon injury. Methods After rabbit arterial carotid injuried, injuried arterial segments were immediately infected by LacZ recombinant adenovirues (AdLacZ) and human p27kipl recombinant adenovirues (Adhp27kip1) in vivo, respectively. Western blot, x-gal stainning, HE stainning, immunochemistry and compute image system were used to analyze the expression of exogenous p27kipl gene and its protein product and the effects on neointimal proliferation. Results Comparison to AdLacZ infected or uninfected arterial segments, Adhp27kip1 infected segments overexpressed p27kipl protein, the peak of expression was in 7-14 d, expression lasted more than 4 weeks. After 4 weeks, in uninfected, AdLacZ infected or Adhp27kipl infected segments, the neointimal area was 1.106 mm2, 0.988 mm2 and 0.278 mm2, respecively; the rate of luminal stenosis was 87.07%, 65.40% and 32.14%, respectively. Conclusion Exogenous p27kipl gene and its protein product overexpression in injuried arterial segments could inhibit neointimal proliferation and luminal stenosis significantly after artery injury.

Although Addison's disease is uncommon, it may contribute to maternal and fetal morbidity and mortality if not treated appropriately. Clinical features, laboratory tests and endocrine function tests which conduce to diagnosis are reviewed. The long-term corticosteroid replacement therapy plays an important role in the treatment. Close monitoring during labor and postpartum, appropriate arrangement of corticosteroid could optimize the maternal and fetal outcome.

Objective To study the adjunctive therapy with mucosolvan oxygen atomization on bronchitis. Methods All of the 83 cases were bronchitis patients in the hospital, and divided into 2 groups, we observed it`s curative effect compared with normal therapy. HZ Results Therapy in hospital mucosolvan group was 8.4 and thutinegroup was 11.2. Time of chirping and disappearing of lung phlegm.It is 5.1 to bathe Mucosolvan group. But routine group was 8.4.The two groups had remarkable differences and have statistics meanings. Conclusion Mucosolvan Oxgyen urge atomization is a kind of safe and high-efficcient way to heal the bronchitis.