The stability and accuracy of dosage monitoring system are crucial to guarantee the dosage output veracity and its curative effect,so it is essential to repeatedly inspect dosage monitoring system.But for the present inspection process,some practical problems exist such as comparative complex inspection operation,complicated and time-wasting data analysis and process,etc.This study not only makes some progress on the methods for index inspection and data analysis & process,but also designs a data analysis & process software based on delphi7.0,which makes the whole inspection process simpler and saves much time.

Objective To investigate the factors related with swallowing function after stroke. Methods Stroke patients with dysphagia were reviewed, 296 cases with pseudobulbar paralysis, and 82 cases with bulbar paralysis. Swallowing function was evaluated with videoflu-oroscopic swallowing study (VFSS). The variable of age, sex, Body Mass Index (BMI), scores of National Institutes of Health Stroke Scale (NIHSS), Simplified Fug-Meyer Assessment (FMA) and modified Barthel Index (MBI), intubation, aspiration, length of hospitalization (LOH), and initiation of rehabilitation intervention were analyzed with Logistic regression. Results Age, NIHSS score, intubation, aspira-tion, initiation of rehabilitation intervention were the risk factors for poor outcome of swallowing function in all the patients, while the score of MBI was the protective factor for the pseudobulbar paralysis ones. Conclusion The younger the patients, the less the NIHSS score, no in-tubation, no aspiration, and the earlier the rehabilitation intervened, the more independent the pseudobulbar paralysis patients, the better swallowing function recovered.

Objective To investigate the effect of intensive education among the families on nutritional status of the stroke patients with dysphagia. Methods 42 stroke patients with dysphagia diagnosed with Standard Swallowing Assessment were enrolled in the study. The patients and their families were randomly divided into intervention group (21 pairs) and control group (21 pairs). The families of the intervention group were given the intensive health education, 5~10 minutes each time, once a week for 1 month. The families of the control group were only given routine health education. The two groups of patients received conventional drug and swallowing function training. Hemoglobin (Hb), serum albumin (ALB), contralateral brachial triceps skin fold thickness (TSF) and arm muscle circumference (AMC) were measured 1 month latter. Results Hb, ALB, TSF and AMC in the intervention group were higher than in the control group (P<0.05). Conclusion Intensive education among the families may improve the nutritional status of stroke patients with dysphagia.

OBJECTIVE: To analyze the conservative management and outcomes of pharyngocutaneous fistula after total laryngectomy. METHOD: Twenty-one patients with postoperative fistulas were identified and treated by conservative therapy. RESULT: Different treatment were given basing on the three stages of pharyngocutaneous fistula: drainage and cleaning stage, pressure bandaging stage and healing stage. Fourteen patients (66.7%) with the conservative therapy resumed oral feeding after closure of fistula, the other 7 patients had to be cured by further operation. CONCLUSION Management basing on the stages of pharyngocutaneous fistula can achieve satisfied outcome. It can provide important information for pharyngocutaneous fistula's treatment.
Carcinoma, Squamous Cell ; surgery ; Cutaneous Fistula ; therapy ; Humans ; Laryngeal Neoplasms ; surgery ; Laryngectomy ; adverse effects ; Pharyngeal Diseases ; therapy ; Postoperative Complications ; therapy

OBJECTIVE:To optimize the formulation of ferulic acid ligustrazine (FATM) solid lipid nanoparticles (FATM-SLN),and conduct the quality evaluation. METHODS:Emulsification ultrasonic method was used to prepare FATM-SLN. Using particle size and entrapment efficiency as indexes,amount of glyceryl monostearate,egg yolk lecithin (PC),poloxamer 188 (P188),and sodium stearate as factors,single factor test and orthogonal test were used to optimize the formulation;and verifica-tion test was conducted. The appearance morphology,distribution of particle size,Zeta potential,stability and in vitro release de-gree of prepared FATM-SLN were investigated. RESULTS:The optimal formulation was as follows as FATM of 10 mg,glyceryl monostearate of 300 mg,PC of 200 mg,P188 of 200 mg,sodium stearate of 10 mg,and purified water of 20 mL. The prepared FATM-SLN showed spherical solid particles,appearance morphology was round,distribution of particle size was 40-800 nm,parti-cle size was 106.23 nm,polydispersity index was 0.254,Zeta potential was -34.8 mV,entrapment efficiency was 73.32%,drug loading was 1.20%;the appearance had no obvious changes within 10 d in 4 ℃(RSD<2%). The drug-release in 0.5-1 h was the fastest,the cumulative release degree reached to 60.47%;it tended to be stable after 8 h,the cumulative release degree reached to 93.46%,and drugs were basically released completely. CONCLUSIONS:FATM-SLN formulation is successfully optimized,and the prepared FATM-SLN has small particle size,high entrapment efficiency and good stability.

OBJECTIVETo investigate the protective effects and related mechanisms of soybean isoflavone (SI) on human umbilical vein endothelial cells (HUVECs) injury induced by H₂O₂ and lipopolysaccharide (LPS).

METHODSH₂O₂ and LPS were used to induce HUVECs injury in vitro. Nine experimental groups were examined: control group, H₂O₂ (2 mmol/L for 4 h), LPS (2 mmol/L for 4 h), H₂O₂+low dose SI (1 mg/ml), H₂O₂+moderate dose SI (2.5 mg/ml), H₂O₂+high dose SI (5 mg/ml), LPS+low dose SI (1 mg/ml), LPS+moderate dose SI (2.5 mg/ml), LPS+high dose SI (5 mg/ml). The survival ratio of HUVECs was detected with MTT assay. The cultured cells were loaded by Fura-2/AM and the change of [Ca²⁺] in HUVECs was measured by fluorospectrophotometry. The contents of malondialdehyde (MDA), superoxide dismutase (SOD), reduced glutathione (GSH-Px) were measured by the commercial kits. The levels of tissue plasminogen activator IL-6 in the supematant were measured by enzyme linked immunosorbent assay (ELISA) kits. Apoptosis rate of the HUVECs was analyzed by flow cytometry.

RESULTSH₂O₂ and LPS significantly decreased HUVECs viability, increased the contents of MDA, IL-6 and decreased the contents of SOD and GSH-Px, and increased the apoptosis rate [(37.8 ± 1.8)% and (38.9 ± 1.1)%]. Co-treatment with SI could reduce MDA and IL-6 while increase SOD and GSH-Px and reduce apoptosis in a dose-dependent manner.

CONCLUSIONThe findings demonstrate that soybean isoflavone could attenuate H₂O₂ and LPS induced injury in human umbilical vein endothelial cells through protecting mitochondrial function, improving antioxygenic activity, and suppressing the mobilization of cytosolic calcium.

Apoptosis ; drug effects ; Calcium ; metabolism ; Cells, Cultured ; Human Umbilical Vein Endothelial Cells ; drug effects ; metabolism ; pathology ; Humans ; Hydrogen Peroxide ; toxicity ; Isoflavones ; pharmacology ; Lipid Peroxidation ; drug effects ; Lipopolysaccharides ; toxicity ; Oxidative Stress ; drug effects ; Soybeans ; chemistry