1.The expression of 53BP1 and 53BP2 gene in nasopharyngeal carcinoma
Hong LI ; Ling ZHANG ; Xuyu YANG ; Weinong HAN ; Wen ZHOU ; Kaitai YAO
Chinese Journal of Pathophysiology 2001;17(3):219-222
AIM: To investigate the expression map of two p53 binding proteins 53BP1 and 53BP2 in nasopharyngeal carcinoma (NPC) tissue.METHODS: The expression of 53BP1 and 53BP2 mRNA in NPC biopsy and control group are tested by RT-PCR. The expression of two mRNA in NPC paraffin section are examined by in situ hybridization. RESULTS: No expression of 53BP1 mRNA was found in NPC tissue and control group. However, expression of 53BP2 was detected in NPC biopsy and control group by RT-PCR, specific expressoin found cancerous nest in NPC paraffin section by in situ hybridization.CONCLUSION: The high expression of 53BP2 may be related to the development of NPC.
2.Plerosis of cDNA array of normal human nasopharyngeal tissue and nasopharyngeal carcinoma.
Weinong HAN ; Hong LI ; Lu XIE ; Liangguo XU ; Ling ZHANG ; Kaitai YAO
Chinese Journal of Oncology 2002;24(2):114-117
OBJECTIVETo compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue.
METHODSAfter hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results.
RESULTSOf 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated.
CONCLUSIONDNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma.
DNA Repair ; genetics ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Nasopharynx ; metabolism ; pathology ; Oligonucleotide Array Sequence Analysis ; methods ; RNA, Neoplasm ; genetics ; Reverse Transcriptase Polymerase Chain Reaction