OBJECTIVE:To systemically review the efficacy and safety of Ambroxol hydrochloride injection by intravenous drip combined with aerosol inhalation in the treatment of neonatal pneumonia,and provide evidence-based reference for the clinical treatment. METHODS:Retrieved from PubMed,Medline,CBM,CJFD,VIP and Wanfang Database,randomized controlled trials (RCT)about Ambroxol hydrochloride injection by intravenous drip combined with aerosol inhalation(test group)versus Ambroxol hydrochloride injection alone (control group) in the treatment of neonatal pneumonia. Meta-analysis was performed by using Rev Man 5.2 software after data extracting and quality evaluating by Cochrane 5.1.0. RESULTS:Totally 14 RCTs were enrolled,involv-ing 1 407 patients. Results of Meta-analysis showed the clinical efficacy[RR=1.19,95% CI(1.13,1.24),P<0.001] in test group was significantly higher than control group,the time of cough disappearance[MD=-1.75,95%CI(-1.91,-1.58),P<0.001], asthma disappearance[MD=-1.38,95%CI(-1.67,-1.08),P<0.001] and pulmonary rales disappearance [MD=-1.44,95%CI (-1.77,-1.11),P<0.001] and hospitalization days[MD=-2.04,95%CI(-2.25,-1.82),P<0.001] were significantly shorter than control group,the differences were statistically significant;and there was no significant difference in the incidence of adverse reactions between 2 groups[RR=1.00,95%CI(0.49,2.05),P=1.00]. CONCLUSIONS:Both the efficacy and safety of Ambroxol hydrochloride injection by intravenous drip combined with aerosol inhalation are good in the treatment of neonatal pneumonia.

Objective To evaluate the biologic characteristics of a new type femoral stems either coated with gradient hydroxyapatite (HA) or additionally loaded with rhBMP-2. Methods Fifteen dogs were divided into 3 groups according to the implanted hip prosthesis. Each animal received hemiarthroplasty in the right hip with prosthesis made of pure Ti-6Al-4V femoral stem (Ti group), the new gradient HA-coated femoral stem (HA group) with 100 to 150 ?m hydroxyapatite coating, and the new gradient HA-coated femoral stem loaded with rhBMP-2 of 9 mg (BMP-HA group) respectively. The dogs were sacrificed at 12 weeks after operation, and labeled with tetracycline 3 of 30 mg/kg days prior to sacrifice, and then the histological changes of the tissue surrounding the prosthesis were evaluated. The radiographic examinations were taken before and after sacrifice. Results On radiographic examination, local radiolucent line around the prosthesis was observed only in one specimen in Ti group. The new bone formation was found both in HA group and BMP-HA group more obviously than in Ti group. Under microscopy, it was noted that this new gradient HA-coating was intact and no obvious biodegradation in most section, whereas a fibrous membrane was found in Ti group. The average rates of interface osteointergration in Ti group, HA group and BMP-HA group were 4.05%?7.66%, 71.04%?9.81% and 88.86%?6.56%, respectively, those of HA and BMP-HA group were significantly higher than that of Ti group (P

[Objective]To evaluate the interface biomechanical characteristics of a new kind of hydroxyapatite coating.[Method] Ten dogs underwent hermiarthroplasty of hip joint and were divided into 2 groups.One group was placed in Ti-6AI-4V femoral stem(Ti hip group),the second group was placed in HA-coated femoral stem(HA hip group).The second method was rod implant in condyles in both legs of each dog.Four matured dogs were selected.Eight Ti-6AI-4V rods(Ti rod group)were placed in lateral and medial condyle in left leg of each dog and eight HA-coated rods(HA rod group)were implanted in that of right leg of each dog.The dogs were sacrificed at 12 weeks after operation,and then the interface shear strength and imerface histological changes were evaluated.[Result]The Pust-out test showed that the average interface shear strengths of Ti hip group and HA hip group were(2.58?1.56)Mpa and(7.57?1.03)Mpa respectively.The interface shear strengths of HA hip group was significantly higher than that of Ti hip group(P

Objective To determine the feasibility of using Tet-on regulatory system to transfect primary rabbit bone marrow stem cells, and to control the activities of the target gene. Methods The Tet-on regulatory system were used to transfect bone marrow stem cells by lipofectamine with the increasing concentration of doxycycline (Dox), the activity of the fluorescence caused by the expression of the target gene (the luciferase gene) which transfected to bone marrow stem cells with Tet-on regulatory system were analyzed. Results Plasmids DNA after being digested by enzymes and agarose electrophoreses were analyzed. The DNA segments were cored with the plasmid map. The two DNA segments with the size of 6.02 kb and 1.38 kb could be detected after the plasmid of pTet-on, 5.2 kb DNA segment could be seen after the plasmid of pTRE-luciferase was digested by Hind Ⅲ. With the increasing concentration of Dox, the activity of the fluorescence caused by the expression of the target gene increased gradually. Although the uninfected bone marrow stem cells were induced, the relative-luciferase-unit(RLU) value was almost the same as that of the blanket control. It showed that uninfected bone marrow stem cells were not induced by Dox. The RLU value in infected bone marrow stem cells before induction was slightly high, but similar with the blanket control. This experiment shed light on that infected bone marrow stem cells showed good response to the induction by Dox. Conclusion With the help of Lipofectamine, the cultured bone marrow stem cells can be transfected by Tet-on regulatory system successfully. The expression of the target gene transfected to bone marrow stem cells with Tet-on regulatory system could be regulated by Dox.

Objective To construct vectors based on adeno-associated virus type 2 (rAAV2) carrying bone morphogenetic protein-7 (BMP-7) and observe their expression in adipose-derived adult stem cells, which can be served as a new gene therapy method and cell source for bone tissue engineering. Methods The coding sequence (1. 3 kb) of BMP-7 was amplified by PCR from the pcDNAl. 1 ( + ) plasmid containing the human BMP-7 cDNA. After purified, the gene fragments were cloned into a plasmid pUC18 and termed plasmid pUC18-hBMP7. The recombinant pUC18-hBMP7 was digested and further ligated to the pSNAV by T4DNA ligase and termed plasmid pSNAV-hBMP7. BHK-21 cells were transfected with the purified pSNAV-BMP7 plasmid according to a standard calcium phosphate precipitation method. The cells were isolated and the integrity of hBMP7 gene was determined by polymerase chain reaction (PCR) using the above PCR primers. To package the virus, stably transfected BHK-21 cells were subsequently infected with recombinant herpes simplex virus type 1 (rHSV-1). The collected cells were processed by chloroform treatment, PEG8000/NaCl precipitation and chloroform extraction for purification. The tiler was determined using quantitative DNA dot blots and the purity was examined by sodium dodecyl sulphatepolyacrylamide gel electrophoresis. Following infection with rAAV2-BMP7 at multiplicities of infection of 1?105 vector genomes per cell and subsequent culture, adipose-derived adult stem cells were assessed qualitatively for BMP7 production. Results The recombinant plasmid pSNAV-hBMP7 was identified by PCR and digested with restriction enzyme. Transfection showed an efficiency of 90 % in ADAS cells. BMP-7 expression in ADAS cells was identified by Western blot. Conclusions The hBMP-7 recombinant adeno-associated virus vectors can be successfully constructed in vitro and rAAV2-hBMP7 can infect ADAS cells.

To study the stability of fal emulsion in all types of AU-in-One nutrient admixtures prepared from on morket fat emnlsions and new MCT/LCTs. Admixtures were prepared from domestic fat emulstions and MCT/LCTs separately with amino acids,dextrose,vitamins, electrolyltes and trace elements. They were stored for one day at 25℃ followed by one day at 25℃ and eight days at 4℃. Admixtures of control group were prepared with fat emulsions and MCT/LCTs of other manufacturers and stored under the same conditions. Particle size and distribution, mass percentage of larger particles to theroretical total iol volume were measured by using light seattering spectrophotometer and coulter multisizer accucomp. Osmolality and pH of the samples were also measured. Stability of the all-in-one admixtures was monitored. The results showe that, compare with the control products, the particle size and distribution of domestic fat emulsions and MCT/LCTs, pH and osmolality of the admixtures remained stable during observation.

OBJECTIVE: To establish the HPLC method for the content determination of baicalin in Xiaoer zhike granule. METHODS: YWG C18 (150 mm?4.6 mm,10 ?m) column was used and the mobile phase consisted of methnol-water-phosphoric acid (47 ∶ 53 ∶ 0.2) with the flow rate of 2.5 mL?min-1. The detection wavelength was set at 280 nm and injection volume was 10 ?L. RESULTS: The linear range of baicalin was 25～200 ?g?mL-1(r=0.999 6) with an average recovery of 99.57%(RSD=1.9%,n=6). CONCLUSION: This method is sensitive, simple and accurate for the quality control of Xiaoer zhike granule.

Objective To review the characteristics and outcomes of primary total hip replacement(pTHR) in patients with ankylosing spondylitis(AS). Methods Eighteen patients (26 hips) with AS underwent pTHR from January 1991 to September 2003 were reviewed retrospectively. Sixteen patients (24 hips) were followed-up. The mean duration of follow-up was 85 months (ranging from 23 to 169 months). All patients were evaluated using Harris score standardization. Results There were 15 hips with hip ankylosis( 57.7%). The average time of the operation was 167.4 minutes (ranging from 90 to 350 minutes),the average volume of bleeding in the operation was 773.9 ml (ranging from 300 to 2000 ml),the postoperative average drainage was 488.6 ml(ranging from 50 to 1130 ml). The rate of excellent and good was 87.5 percent. Conclusion The pTHR is a very effective treatment for patients with AS. The perfect rehabilitation care is one of the main factors influencing the outcomes of pTHR for patients with AS.

Objective To observe the effect of arthroscopic meniscectomy for symptomatic discoid lateral meniscus and the importance of early treatment. Methods Arthroscopic meniscectomy was performed on 29 patients (31 knees) with symptomatic discoid lateral meniscus. Partial meniscectomy was performed on 27 knees, subtotal meniscectomy on 4 knees with tear on the edge of meniscus. Results The operations on all 31 knees were successful without complications. The mean Lysholm score was 95.6 (87-100) during the follow-up, which was significantly higher than that before the operation (65.7, range 36-78; P

Objective To investigate the effect and clinical valve of target-controlled infusion of remifentanil for conscious intubation of patients under fibrobronchoscopy.Methods Forty patients were randomly divided into two groups(F and R),each group contained 20 cases.All of the patients were intubated on regional anaesthesia,as the first step,then the patients in F group were given fentanyl 2?g/kg by vein.In remifentanil target-controlled infusion group(R group),the patients were kept infusing remifentanil 2ng/ml.Results (1)Haemodynamics:in R group,SBP and HR kept steadily;In F group,while the two indices increased significantly.(2)Static pulmonary function:in R group,RR,VT deceased remarkablely.There were no difference between two groups in SpO_2 and P_ ET CO_2.(3)Tracheal intubating condition and Ramsay score in R group were better than in F group;Cardiovascular response in R group was lower.Conclusion Target-controlled infusion of remifentanil for conscious intubation can achieve excellent result,with less cardiovascular response.