Objective:To search for therapeutic effect of lidocaine in the treatment of acute episode of chronic asthmatic bronchitis.Methods:The 58 cases of patients with acute episode of chronic asthmatic bronchitis admitted to this hospital from August,2004 to July,2007 were divided into group A and group B.Besides group A with lidocaine and group B as control with sodium chloride solution,there were no difference in the other therapeutic measures between group A and Group B.Oxygen was used as the power of spray or aerosolizer,the flow volume of oxygen was 8-10L/min.Group A patients were given 5 milliliters of 2% lidocaine(100mg) by nebulized inhalation three times a day for three days,and Group B patients received 5 milliliters of 0.9% sodium chloride solution by nebulized inhalation three times a day for successive three days.The changes of heart rate,respiratory frequency,forced expiratory volume in one second(FEV1),peak expiratory flow(PEF),partial pressure of oxygen in artery(PaO2) and partial pressure of carbon dioxide in artery(PaCO2) were determined in the group A and B before and after the nebulized inhalation therapy.Results:The results of the heart rate,respiratory frequency,FEV1,PEF,PaO2 and PaCO2 before and after the treatment of the nebulized inhalation were showed as follows,improvement of heart rate and respiratory frequency was more significant in the group with the nebulized inhalation treatment of lidocaine than that in the group as control after 30 minutes,one day and three days of therapy(P

Objective:To discover the risk factors,clinical features,treatment and prognosis of fungal infection in the lungs.Methods:The data of 103 patients suffering from fungal infection in the lungs were analyzed retrospectively.Results:There were ninety percent(91/103) of patients with underlying diseases,mainly chronic obstructive pulmonary disease(COPD) and chronic cor pulmonale.68(66.02%) patients suffered from two or more than two kinds of underlying diseases.Type 2 diabetes also took an important position in all underlying diseases.Clinical manifestations of pulmonary fungal infection showed no specificity.X-ray appearance showed that bronchopneumonitis was predominated(60%).The fungal culture results showed that blastocystis was the major pathogenic fungus,including 73(70.8%)strains of Candida albicans,11(10.7%) strains of Candida tropicalis,10(9.7%) strains of Torulopsis glabrata,6(5.8%) strains of aspergilli and 3(2.9%)trains of mold fungus,and early diagnosis of this disease remained difficult.The results of sputum cultuer showed that 48(46.6%) patients suffered from pumomary fungal infection combined with other bacterial infections,such as Pseudomonas aeruginosa,Acinetobacteria,Methicillin-resistant Staphylococcus aureus(MRSA),Escherichia coli and so on.The mortality of pulmonary fungal infection in this group was high(21.36%).6 patients died from uncontrolled fungal infections in the lungs and other16 patients died from mutiple organ failure due to delayed antifungeal therapy.Conclusions:Pulmonary fungal infection with few specific clinical manifestations and high mortality was an important cause of secondary infections in the patients suffering from many kinds of diseases,which is worthy of more attention in clinic.

Objective To study the causes and methods of prevention and treatment of severe acute pancreatitis (SAP) complicated with postoperative duodenal fistula. Methods Twenty-two cases with severe acute (pancreatitis) complicated with postoperative duodenal fistula were retrospectively analyzed . Results Among 184 patients with severe acute pancreatitis undergoing operative treatment,duodenal fistula developed in 22 (patients),and in 15 of the 22 (patients), it occurred 2 weeks after operation.In 18 patients the duodenal fistula healed spontaneously with conservative therapy and 4 patients were cured by re-operation. Conclusions (Duodenal) fistula is related to early operation for SAP,peripancreatic infection,and improper placement of (drainage) tube at time of operation or (prolonged) (placement) of (drainage) tube.Most duodenal fistulas can be (spontaneously) cured by maintaining patent drainage around the fistula, actively controlling peripancreatic (infection), suppression of gastrointestinal secretion, and augmention of nutritional support.

Objective　To study the role of α1-antichymotrypsin(α1-ACT)in the pathogenesis of childhood asthma for providing a theoretical base for prevention and treatment of childhood asthma. Methods　Levels of plasma α1-ACT in 30 asthmatic children and 30 healthy children were determined by rocket immunoelectrophoresis. Results　Plasma α1-ACT levels were higher in acute phase than in paroxysmal phase ［（0.4178±0.0815）vs(0.2865±0.0799), t=6.301,P<0.001］ and also higher in asthma group than in control group ［（0.4178±0.0815）vs(0.2876±0.0496),t=7.474,P<0.001］. The plasma α1-ACT levels in asthma group and control of paroxysmal phase were similor ［(0.2865±0.0799) vs (0.2876±0.0496),t=0.064, P>0.05］. The levels of plasma α1-ACT were higher in moderate and heavily attacked patients than that in mild attacked patients ［(0.4553±0.0758) vs (0.3249±0.0926),t=4.187,P<0.001］ and those in control group ［(0.4553±0.0758) vs (0.2865±0.0799),t=9.38,P<0.001］. Conclusion　α1-ACT is involved in pathophysiological course of childhood asthma. Measurement of plasma α1-ACT levels may be helpful in determining the state of the illness and to evaluate the severity and treatment of childhood asthma.

PBL works as a method based on problems,and its theory essence is the con-structivism.With the adjustment of the educational aim,the definition of faculty quality need change correspondingly.Under PBL medical education mode,"faculty"is no longer defined as the traditional"teacher",but as the promoter of medical knowledge for students and the participants of medical type reform.These two are the main characters of PBL medical faculty.

OBJECTIVE:To review the relationship between endothelial progenitor cells and the re-endothelialization of vascular prosthesis in order to find out some effective ways to solve the most frequent complications of asotransplantation,thrombogenesis and intima hyperplasia.DATA SOURCES:A computer-based online search was conducted to identify articles related to endothelial progenitor cells and the re-endothelialization of vascular prosthesis published in Pubmed,Ovid and MD Consult database from January 2000 to December 2006 using the key words of "endothelial progenitor cells,vascular prosthesis,endothelialization".Meanwhile,CNKI database was searched for related papers published between January 2000and December 2006,the keywords were "endothelial progenitor cells,vascular prosthesis,endothelialization" in Chinese.STUDY SELECTION:The literatures included all the related papers about the roles of endothelial progenitor cells in the re-endothelialization of vascular prosthesis.Inclusive criteria:the study types were randomized controlled trials,drug stress test and clinical drug effect test; the samples were both human and animals.Exclusive criteria:Reviews and literatures without controls were excluded.DATA EXTRACTION:Totally 115 related literatures were collected,and 24 were accorded with the inclusive criteria.The excluded were 91 papers of reviews and repeated trials or drug effect studies.DATA SYNTHESIS:These related literatures,including not only animal experiments but also clinical detections,analyzed the relationship between endothelial progenitor cells and the re-endothelialization of vascular prosthesis and correlative promoting mechanisms.CONCLUSION:It is concluded that endothelial progenitor cells play an important role in the endothelialization after vascular prosthesis is grafted in vivo.

Iodine is a micronutrient that is essential for the production of thyroid hormones.Either iodine deficiency or excess will not only affect the synthesis and secretion of thyroid hormone but also be related to the occurrence and development of thyroid diseases.Iodine deficiency can cause endemic goiter,cretinism,hypothyroidism and thyroid cancer;high incidence of hyperthyroidism,hypothyroidism,autoimmune thyroid disease,goiter,and thyroid cancer are associated with excessive iodine intake.It is essential to assess iodine nutritional status for the populations,particularly those with thyroid diseases.

Objective To evaluate the biologic characteristics of a new type femoral stems either coated with gradient hydroxyapatite (HA) or additionally loaded with rhBMP-2. Methods Fifteen dogs were divided into 3 groups according to the implanted hip prosthesis. Each animal received hemiarthroplasty in the right hip with prosthesis made of pure Ti-6Al-4V femoral stem (Ti group), the new gradient HA-coated femoral stem (HA group) with 100 to 150 ?m hydroxyapatite coating, and the new gradient HA-coated femoral stem loaded with rhBMP-2 of 9 mg (BMP-HA group) respectively. The dogs were sacrificed at 12 weeks after operation, and labeled with tetracycline 3 of 30 mg/kg days prior to sacrifice, and then the histological changes of the tissue surrounding the prosthesis were evaluated. The radiographic examinations were taken before and after sacrifice. Results On radiographic examination, local radiolucent line around the prosthesis was observed only in one specimen in Ti group. The new bone formation was found both in HA group and BMP-HA group more obviously than in Ti group. Under microscopy, it was noted that this new gradient HA-coating was intact and no obvious biodegradation in most section, whereas a fibrous membrane was found in Ti group. The average rates of interface osteointergration in Ti group, HA group and BMP-HA group were 4.05%?7.66%, 71.04%?9.81% and 88.86%?6.56%, respectively, those of HA and BMP-HA group were significantly higher than that of Ti group (P

Objective To determine the feasibility of using Tet-on regulatory system to transfect primary rabbit bone marrow stem cells, and to control the activities of the target gene. Methods The Tet-on regulatory system were used to transfect bone marrow stem cells by lipofectamine with the increasing concentration of doxycycline (Dox), the activity of the fluorescence caused by the expression of the target gene (the luciferase gene) which transfected to bone marrow stem cells with Tet-on regulatory system were analyzed. Results Plasmids DNA after being digested by enzymes and agarose electrophoreses were analyzed. The DNA segments were cored with the plasmid map. The two DNA segments with the size of 6.02 kb and 1.38 kb could be detected after the plasmid of pTet-on, 5.2 kb DNA segment could be seen after the plasmid of pTRE-luciferase was digested by Hind Ⅲ. With the increasing concentration of Dox, the activity of the fluorescence caused by the expression of the target gene increased gradually. Although the uninfected bone marrow stem cells were induced, the relative-luciferase-unit(RLU) value was almost the same as that of the blanket control. It showed that uninfected bone marrow stem cells were not induced by Dox. The RLU value in infected bone marrow stem cells before induction was slightly high, but similar with the blanket control. This experiment shed light on that infected bone marrow stem cells showed good response to the induction by Dox. Conclusion With the help of Lipofectamine, the cultured bone marrow stem cells can be transfected by Tet-on regulatory system successfully. The expression of the target gene transfected to bone marrow stem cells with Tet-on regulatory system could be regulated by Dox.

ObjectiveTo observe the efficacy of rehabilitation therapy for patients and experimental animals with short bowel syndrome.MethodsThirty SD rats were randomly divided into control group,short bowel group and growth hormone (GH) treatment group.Short bowel was produced by resecting 80% of the rats′ small intestine, growth hormone at a dosage of 1?U/kg?d was delivered to the rats from POD1 to POD28.Nine patients with short bowel syndrome were enrolled in the study,the average length of the small intestine was (44?24)?cm, 3 cases had also suffered a short colon.ResultsIncreased ratio of S phase cells, proliferation index and expression of proliferative cell nucleus antigen (PCNA) were observed, indicating the putative effects of exogenous GH on residual small intestinal mucosa. The mechanism of action was probably through the alteration of protooncogene C-jun. The nutritional state of the patients and the absorptive capacity of the residual intestine improved markedly. Follow up were made to 8 patients,75% of the patients have weaned TPN,25% of them need intermittent TPN supplementation.ConclusionsRehabilitation therapy provides a new and effective alternative for short bowel patients.