Objective To develop a mobile terminal data acquisition for data collection of malaria prevention and control in the field. Methods Based on 3G,Bluetooth,GPS and/or Bei Dou Navigation Satellite System(BDS)and wireless network communication technology(WIFI),a mobile terminal data acquisition for data collection of malaria prevention and control was developed. Results The developed system included 2 functional modules:a terminal and iCloud,and had examination,modi?fication and deletion functions of malaria epidemiological data. By using GPS and GIS,the target population or institution could be accurately positioned to determine whether the distribution of malaria cases had a spatial aggregation. Conclusion A mobile terminal data acquisition in malaria prevention and control is successfully developed,which is very convenient for data collec?tion and submission of malaria prevention and control.

Objective To evaluate the influence of angiotensin converting enzyme (ACE) gene polymorphism on the effect of aerobic endurance training of soldiers.Methods Two hundred and forty-eight army recruits (males,Hans) from an infantry regiment without previous history of military training were involved in the present study,and they were divided into experimental group and control group (124 each).Intensified endurance training designed with the theory of "cyclic training" was applied in experimental group,and routine training method was applied in control group including mainly 5000 meters running for 3 times in one week (no more than 1 time per day).The 5000m running performance and maximal oxygen consumption (VO_2max) were determined before and after eight-week training as endurance capacity indices.The ACE gene polymorphism was determined and the recruits in both groups were divided into 3 sub-groups according to the ACE genotypes as II,ID and DD.The influence of ACE genotype and training method on endurance capacity was analyzed.Results The 5000m running performance and VO_2max of experimental group after eight-weeks-training were significantly higher than those before training and those of control group (P0.05).In control group,the 5000m running performance and VO_2max of DD sub-group were significantly lower than those of II sub-group and ID sub-group (P0.05).Conclusions Intensified endurance training designed with the theory of "cyclic training" may effectively improve the endurance capacity in 8 weeks.ACE genotype could not be used as an accurate indicator to evaluate the endurance capacity and improvement of efficiency of soldiers.

Objective To explore an appropriate training method to quickly improve the soldiers' aerobic endurance capacity and decrease the incidence of military training injuries for combat troops. Methods Two hundred and forty-eight recruits (males,Hanzu) from infantry without previous history of military training were involved in the present study,and they were divided averagely into experimental group and control group (124 each). Intensified endurance training designed with the theory of "cyclic training" was applied in experimental group,and routine training method was applied in control group including mainly 5000 meters running (no more than once a day,3 times a week). The resting pulse,vital capacity,maximal oxygen consumption (VO2max) and the performance of 5000m running were determined before and after eight-week-training as endurance capacity indices. Physical examination was performed to count the cases of military training injuries including calf swelling and tenderness,knee swelling and pain,and low back pain,and then the incidence of military training injuries was calculated. Results In the experimental group,the resting pulse decreased by 8.0%,while the vital capacity,VO2max and 5000m running performance elevated by 8.1%,20.0% and 14.7% respectively after training,which were significantly different in comparison with that before training and of control group (P

Objective]Not only to promote the rational allocation of resources between government, society and universities,but also to provide theoretical basis and practical path for connotational development of newly-built undergraduate college.[Methods]Focusing on“ the development situation and the demand for the connotational development of the newly-built undergraduate college”and“ the aid possibility”,five representatives of newly-built undergraduate colleges and seven aid-donors were interviewed and inquired by Likert scale .[Results]The average index of newly-built undergraduate colleges on“ the development situation” and “ the demand for the connotational development” are respectively 2.93 and 4.03,showing a good situation and strong demand for external assistance.Meanwhile, the aid-donors also show a high will on the aid possibility” ,the average index is 4.06.[Conclusion]Based on analysis of“ the development situation and the demand for the connotational development of the newly-built undergraduate college, andthe aid possibility”,our research puts forward aid model and mechanism in connotational development of newly-built undergraduate college in order to make full use of external assistance, including government,society and universities.

Objective To analyze the clinical efficacy of lateral elbow approach combined with anterior median approach in the treatment of terrible triad of elbow.Methods Retrospectively analyzed 20 patients of terrible triad elbow who were admitted into our hospital from April 2012 to January 2015 and treated with lateral elbow approach combined with anterior median approach.The postoperative recovery of arthrosis,forearm rotation range,and range of motion were evaluated by Mayo score system.Results All patients wounds were with primary healing.There were 11 cases of excellent and 4 cases of good through Mayo score system,with the good rate of 75％.At the final follow-up,the range of motion was (122.06 ± 24.17) ° and the forearm rotation was (136.28 ± 8.9) °.Conclusion Lateral elbow approach combined with anterior median approach for the treatment of terrible triad of elbow has clear visual field,excellent surgical rate,without complications such as elbow instability and nerve damage.

Objective To explore the effect of rational emotive therapy on self-esteem status of breast cancer patients.Methods 118 cases of breast cancer patients were randomly divided into the control group and the observation group with 59 cases in each group.The control group received routine care,and the observation group received rational emotive therapy on the basis of routine care.The self-esteem status of the two groups of patients was assessed before and after the intervention respectively with State Self-Esteem Scale (SSES).Results The score of behavior self-esteem,social self-esteem,appearance self-esteem and the total score of self-esteem of the patients in two groups before intervention showed no significant difference,but the above scores were higher in the observation group than those in the control group after intervention.Conclusions Rational emotive therapy can improve self-esteem status of breast cancer patients,thereby improve the mental health of the patients.

Objective To guide the implementation of home enteral nutrition (EN) for outpatients with Crohn's disease, and to observe its effect.Methods In this observational study, 26 patients at active period of Crohn's disease were collected between April 2014 and June 2015 in the clinic of Clinical Nutrition Center of Nanjing General Hospital of Nanjing Military Command.Home EN combined with medication was administered.The nutritional status, Crohn's Disease Activity Index (CDAI), and quality of life before and after EN were compared.Results After home EN therapy, body mass index (BMI) of the patients increased from (17.7 ± 2.1) kg/m2 to (19.8 ± 2.0) kg/m2 (P ＜ 0.001), proportion of lymphocytes from (0.216 ± 0.066) to (0.305 ± 0.106) (P =0.007), albumin level from (40.3 ± 5.9) g/L to (43.7 ± 5.4) g/L (P =0.038), prealbumin from (233.1 ± 71.5) mg/L to (306.0 ± 72.1) mg/L (P =0.009), and transferrin from (2.7 ± 0.6) g/L to (3.1 ±0.7) g/L (P =0.038).In the indicators reflecting the activity of Crohn's disease, CDAI declined from 197.0 ±55.8 to 113.2 ±33.4 (P ＜0.001) after EN, C-reactive protein from 28.50 (18.00-32.80) mg/L to 2.00 (0.58-6.33) mg/L (P ＜0.001), erythrocyte sedimentation rate from (29.6 ± 9.9) mm/h to (9.4 ± 7.6) mm/h (P ＜ 0.001).The patient's disease activity scores and inflammatory reaction indexes all reached the normal levels after EN, show that the patients were in remission stage.In inflammatory bowel disease quality of life score, the score of intestinal symptoms rose from 41.2 ± 13.3 to 57.0 ±9.6 (P ＜ 0.001), the score of systemic symptom from 20.7 ± 5.5 to 28.9 ± 4.2 (P ＜ 0.001), emotional ability from 43.6±16.1 to 61.0 ± 15.9 (P=0.002), and social ability from 20.6 ±4.9 to 28.4 ±5.6 (P＜0.001), all showing statistical significance.Conclusions Standardized home enteral nutrition in patients at active period of Crohn's disease, when implemented under professional guidance of nutritional support team and well followed up, may improve nutritional status, induce remission, and improved quality of life.

Objective To explore the protective effects of adipose - derived stem cells (ADSCs) with phosphodiesterase 5 inhibition by lentivirus-mediated stable gene silencing on the proliferation and apoptosis of renal tubular epithelial cells induced by ischemia-reperfusion injury in vitro. Methods To isolate cultivate and indentify ADSCs from rats. Lentiviral expression vector of carrying PDE5 shRNA gene was transfected into ADSCs, and a negative control group was set up.Western blotting was used to detect PDE5 protein expression levels. ADSCs were co-cultured with NRK-52E in a transwell system, and NRK-52E cells were treated with ischemia/reoxygenation protocol. Edu assay was performed to evaluate the proliferation of NRK cells, flow cytometry to detect the apoptosis of NRK cells, and ELISA to quantify the protein expressions of fibroblast growth factor (FGF) and hepatocyte growth factor (HGF). The expression of E - cadherin and cytokeratin 18 (CK18) was quantified by real time PCR and flow cytometry. Results Western blotting for PDE5 protein indicated a significant reduction of PDE5 protein levels in PDE5 shRNA transduced population. After the treatment of ischemia/reoxygenation in vitro, the proliferative viability and apoptosis of NRK-52E cells co-cultured with ADSCs induced by PDE5 gene inhibition were significantly improved, compared to the normal group (all P<0.05). And the release of HGF, FGF were markedly enhanced (all P<0.05). Moreover, the NRK-52E cells survival, the expression of E-cadherin and CK18 on PDE5 inhibited ADSCs co-cultured with I/R injured NRK cells was significantly increased compared to that in the negative control group (all P<0.05). Conclusion ADSCs preconditioned by inhibition of PDE5 can be a powerful novel approach to improve the survival of renal tubular cells following ischemia-reperfusion injury, and have an obvious tendency to transform epithelial cells.

OBJECTIVE: To prepare clomipramine hydrochloride sustained release tablet and study its in vitro release rate.METHODS: Orthogonal experiment was carried out to optimize the formulation and the preparation was prepared taking by the formula dosage of HPMC,lactose and amylum pregelatinisatum as factors and the in vitro release rate as index.The in vitro drug release rate was investigated as well.RESULTS: The optimal formulation was as follows: HPMC 45 mg,lactose 35 mg,and amylum pregelatinisatum 40 mg.The preparation prepared in the optimal formulation had a sustained release of 24 h and the release behavior of the tablets followed the zero order equation.CONCLUSION: The formula of the sustained release tablets is reasonable and which had satisfactory sustained release efficacy.

Objective To study the effects of Vitamin D (VitD) supplementation on growth and immune function in neonatal mice.Methods A total of 120 mice were divided into four groups (30 mice in each group) according to dose of VitD.The high-dose group,medium-dose group and low-dose group was given 3.44,1.72,0.86 IU VitD drops,respectively.The control group was not treated with VitD drops.Rat body weight,level of peripheral blood 25-(OH)D3 were observed.The cellular immune function (determined by using delayed hypersensitivity reaction experiment),humoral immune function (assessed by antibody producing cells counts and HC50 determination) and mononuclear-macrophage phagocytic function (assessed by mouse peritoneal macrophage phagocytosis of chicken red blood cells test and carbon clearance test) were detected.The flow cytometry assay was carried out to differentiate T lymphocyte subsets.Results With the increase of dose of VitD,levels of peripheral blood 25-(OH)D3 and calcium ion were gradually increased,there were statistically significant differences when compared with the control group(P＜0.05);the body weight and body length were gradually increased,while no statistically significant difference was found among the groups treated with VitD(P＞0.05).Compared to the control group,the toes swelling,phagocytic percentage,number of antibody producing cells,serum soluble HC50 in the high-dose group and medium-dose group were increased significantly,while carbon clearance test phagocytic index were decreased significantly (P＜0.05).With the increase of dose of VitD,the number of CD4+,CD8+ T lymphocytes and the CD4+/CD8+ ratio were gradually increased.Conclusion VitD could promote the growth and development of offspring mice,and enhance the immune function of the body.