Objective:To investigate the expression of recombined human immunoglobulin ? light chain 022 and TOM1 gene and to determine the protein expression of HSIGVL022 in omental adipose tissues of patients with type 2 diabetes and insulin resistance(T2DM-IR).Methods: Fat tissues from greater omentum of T2DM-IR patients and normal controls were obtained.The mRNA levels of recombined human immunoglobulin ? light chain 022 and TOM1 were measured by fluorescent quantitative RT-PCR;expression of HSIGVL022 protein was identified by immunohistochemistry.Results: The mRNA levels of HSIGVL022 and TOM1 in T2DM-IR patients were(34 140?6 160) copy/million house-keeping genes and(4 440?617) copy/million house-keeping genes,respectively;those in control group were(5 930?661) copy/million house-keeping genes and(1 360?82) copy/million house-keeping genes,respectively.There were significant difference between the 2 groups(P

Objective To observe the effect of Fuzheng Hunao capsule on TNF-? and HSP70 protein expression of myocardial tissues in rats with intracerebral hemorrhage.Methods Seventy two rats were randomly divided into 4 groups:sham,model,Fuzheng Hunao and Angong Niuhuang.The protein expression of TNF-? and HSP70 in rat myocardial tissues were detected by the immunohistochemistiy method,and the integrated optical density (IOD) was measured with image analysis.Result The IOD of TNF-? and HSP70 in model group was higher than that in sham group.The IOD of TNF-? in Fuzheng Hunao and Angong Niuhuang group was lower than that in model group.While the IOD of HSP70 was higher than that in model group.Conclusion Inflammatory response attend the myocardial damage.Fuzheng Hunao capsule could protect the myocardial tissues by inhibiting the protein expression of TNF-? and increasing that of HSP70.

Objective To study the chemical constituents of the medicinal fungus Shiraia bambusicola.Methods Chromatography on silica column and preparative HPLC were used to purify the compounds;Spectroscopic methods including 1H-NMR,13CNMR,and MS were used to elucidate their structures.Results Nine compounds were isolated and identified as 3,6,8-trihydroxy-l-methylxanthone(1),3,8-dihydroxy-6-methoxy-1-methylxanthone(2),2,3,6,8-tetrahydroxy-l-methylxanthone(3),3,4,6,8-tetrahydroxy-l-methylxanthone(4),pregn5(10)-en-3?,17?,20?-triol(5),macrosphelide A(6),(+)-griseofulvin(7),griseophenone A(8),and 11,11′-dideoxyverticillin A(9).Conclusion Compounds 1-9 are obtained from the fungus S.bambusicola for the first time.

Objective To measure the scope of normal meniscus by magnetic resonance imaging(MRI),and create a database of meniscus size of each part.Methods The MRI image measured rake angle and back angle,body height and width of each part and the angular distance of rake and back angle of 303 meniscus of normal knee joint.Results The width and height of anterior horn,anterior exterior angle,body,angulus posteriolateralis and back angle of the medial meniscus were (5.67 ± 1.37) mm and (3.53 ± 0.86) mm,(7.24 ± 1.91) mm and (4.38 ±1.01) mm,(5.61 ± 1.73)mm and (4.25 ±1.07) mm,(11.01 ±3.53) mm and (5.41 ± 1.66)mm,(9.21 ±2.71)mm and (5.02 ± 1.39)mm,respectively.Rake and back angle of angular distance was (38.51 ± 10.88)mm.The width and height of the anterior horn,anterior exterior angle,body,angulus posteriolateralis and back angle of the lateral meniscus were (6.95 ± 1.69) mm and (3.83 ± 0.91) mm,(7.48±1.90)mmand (4.06 ± 0.97) mm,(6.51± 1.79) mm and (5.10±1.01)mm,(7.62±1.99)mm and (5.18±1.19)mm,(6.68 ± 1.41) mm and (5.64 ± 1.12) mm,respectively.The rake and back angle of angular distance was (38.51 ± 10.88) mm.Conclusion In this study,we obtained the data of meniscus for normal people by MRI,which provides reference values for diagnosis of meniscus injury and transplantation.

Objective To establish the quality standard of Heibu ointment. Method The contents of Galla Halepensis were determined by TLC, and Gallic Acid was determined by HPLC. Results In TLC, the spots were very clear and specific to identify Heibu ointment. The linear range of Gallic Acid was 0.1～1.1 ?g. The quality standard of Heibu ointment was established. Conclusion The method can be used for quality control of Heibu ointment.

Objective To seek optimum extraction conditions of Radix Scutellaria in Qinbai ointment by studying on extraction process. Method The optimum extraction regard baicalin as the index. It was investigated by the orthogonal design and determined by HPLC. Result The effect of alcohol concentration has extremely remarkable difference (P

Objective To observe the effect of Fuzhenghunao capsule on NE of the locus ceruleus and myocardial tissues in rats with intracerebral hemorrhage. Methods 72 rats were randomly divided into 4 groups:sham,model,Fuzhenghunao and Angongniuhuang. The content of NE were tested by the high performance liquid phase-electrochemical method at 6 h,24 h and 72 h of post-operation. Result NE was obviously increased in sham group,and decreased in two treatment groups. Conclusion Fuzhenghunao capsule could be used to inhibit the activation of locus ceruleus noradrenalin system and protect myocardial tissues by regulating NE content.

AIM:To explore clinical effect of Zhongfeng Fangzhiling Granule(Radix Pseudostellariae,Radix Polygoni Multiflori praeparata cum succo glycines sotae,Hirudo,etc.) on acute cerebral thrombosis.METHODS:72 patients were randomly divided into 2 groups.The treatment group was treated with Zhongfeng Fangzhiling Granule,the control group was treated with Zhilong Xuetong Capsule.The treatment effects between two groups were compared.RESULTS:The effect in treatment group was superior to that in control group.CONCLUSION:Zhongfeng Fangzhiling Granule has significiant therapeutic effect on patients with acute cerebral thrombosis,and the preparation method is simple,so it will be a prospective herb granule.

Objective To evaluate the clinical efficacy of primary intracoronary stenting in the infarct related artery in elderly patients after acute myocardial infarction(AMI). Methods Percutaneous transluminal coronary angioplasty(PTCA) plus primary coronary stenting was performed for 41 AMI patients(≥70 years) within 12 hours from the onset of chest pain. Infarct related arteries were left anterior descending artery in 21 patients(51 2%), left circumflex in 7(17 1%), right coronary artery in 13(31 7%). Thrombolysis in myocardial infarction (TIMI) showed that blood flow was grade 0 in 29 patients, grade 1 in 7, grade 2 in 5. Results Forty eight stents were implanted in the infarct related artery for 41 patients. One patient died of cardiac shock during the procedure, and the success rate was 97 6%(40/41). Grade 3 of blood flow after TIMI was gained in 37 patients(90 2%). One patient died of renal failure while hospitalizing. The clinical successful rate was 95 1%(39/41). Thirty six patients were followed up, 1 patient with restenosis of infarct related artery was performed cutting balloon angioplasty, and 1 patient with congestive heart failure was rehospitalized. Other 34 patients did not manifested angina pectoris. Conclusions Primary coronary stenting for the infarct related artery in elderly patients after AMI is safe and effective with a high successful rate and less complications.

Inhibitory effects of the O - ( 1 - ethoxvl - butyl ) berbarmine (EBB) on cultured fibroblast was studied by observating calmodulin (CaM ) content of cultured fibroblast with ELISA and DNA content at each phase of cell cycle with flow - cvtometerv. The CaM con-tent in the test group . compared to control . decreased markedly and DNA content increased significantly in the G0+Gi phase but reduced in the S phase. These results suggested that inhibitory mechanism of EBB on fibroblast proliferation may be closely related to CaM decrease in cells.