Anandamide (N-arachidonoylethanolamide), an arachidonic acid derivative, is an endogenous ligand for cannabinoid receptors, which are m embers of the G protein (Gi)-coupled receptors family. Ananamide is mainly foun d in central nervous system, immune system and uterus etc and mimics most of the effects of (－)Δ9-tetrahydrocannabinoid ［(－)Δ9-THC］, a psyco active derivative of marijuana. Fatty-acid amide hydrolase (FAAH), which is inv olved in hydrolyzing anandamide to arachidonic acid and ethanolamide, may quickl y regulate level of anandamide in vivo.

Objective To study the chemical constituents of the medicinal fungus Shiraia bambusicola.Methods Chromatography on silica column and preparative HPLC were used to purify the compounds;Spectroscopic methods including 1H-NMR,13CNMR,and MS were used to elucidate their structures.Results Nine compounds were isolated and identified as 3,6,8-trihydroxy-l-methylxanthone(1),3,8-dihydroxy-6-methoxy-1-methylxanthone(2),2,3,6,8-tetrahydroxy-l-methylxanthone(3),3,4,6,8-tetrahydroxy-l-methylxanthone(4),pregn5(10)-en-3?,17?,20?-triol(5),macrosphelide A(6),(+)-griseofulvin(7),griseophenone A(8),and 11,11′-dideoxyverticillin A(9).Conclusion Compounds 1-9 are obtained from the fungus S.bambusicola for the first time.

BACKGROUND:Studies have shown that genetical y modified Schwann cel s can survive for a longer time in vivo, and promote nerve regeneration and functional recovery. OBJECTIVE:To transfect human telomerase reverse transcriptase (hTERT) gene into rat Schwann cel s cultured in vitro via PLXSN vector, and to detect the telomerase activity and biological characteristics of Schwann cel s. METHODS:Schwann cel s from Wistar rats were cultured in vitro and transfected by PLXSN vector with (hTERT group) or without hTERT (empty vector group). Normal Schwann cel s were selected as control group. RT-PCR and western blot methods were used to detect the hTERT protein and mRNA levels in Schwann cel s, and flow cytometry was used to measure the cel cycle distribution. Cel growth was observed by cel growth curve and MTT colorimetric method. RESULTS AND CONCLUSION:At 48 hours after transfection, the mRNA and protein expressions of hTERT were remarkably seen in Schwann cel s. Compared with the control and empty vector groups, the cel s grew faster, the number of cel s at G 0/G 1 Schwann cel s cultured in vitro. phase was reduced, but the number of S phase cel s was increased in the hTERT group (P<0.05). These findings indicate that PLXSN vector-mediated hTERT transfection of Schwann cel s can significantly improve the activity of telomerase in Schwann cel s as wel as promote the proliferation of Schwann cells cultured in vitro.

Objective To study the radiographic findings of anomalies of thoracic skeletion in patients with congenital heart disease.Methods Frontal and lateral chest films of 252 cases with congenital heart disease proved by operation were reviewed.Results The skeletal anomalies in 8 cases including generalized sternal prominence,sternal bowing,pouter pigeon breast,hemivertebrae and butterfly vertebrae of thoracic spine,and deformities of ribs were discovered.Conclusion The skeleton anomalies which are divided into primary and secondary types often occur in patients with congenital heart disease.

Inhibitory effects of the O - ( 1 - ethoxvl - butyl ) berbarmine (EBB) on cultured fibroblast was studied by observating calmodulin (CaM ) content of cultured fibroblast with ELISA and DNA content at each phase of cell cycle with flow - cvtometerv. The CaM con-tent in the test group . compared to control . decreased markedly and DNA content increased significantly in the G0+Gi phase but reduced in the S phase. These results suggested that inhibitory mechanism of EBB on fibroblast proliferation may be closely related to CaM decrease in cells.

hMSH6 is one of the most important members in the mismatch repair family. Its polymorphism is closely related to the pathogenesis and development of neoplasm, particularly in colorectal cancer and endometrial cancer. Moreover, it has been suggested to play a more important role in endometrial cancer compared with hMLH1 and hMSH2.

Objective To investigate the relationship between HBV-DNA level during the course and progress tO cirrhosis in patients with chronic hepatitis B.Methods From 2001 to 2007,a total of 239 chronic hepatitis B patients confirmed by liver biopsy were followed up for a median time of 28 months.HBV-DNA level was measured at baseline and end point.Results Those who progressed to cirrhosis were older and with higher HBV-DNA levels at the end point.Kaplan-Meier analysis showed that the higher the HBV-DNA level at end point,the higher the risk to cirrhosis(X2=11.736,P=0.019).There was no difference between patients with and without cirrhosis at baseline of HBV-DNA level(P=0.531).The Cox regression indicated that the independent risk factors of cirrhosis were as followings:HBV-DNA level at the end point,stage of fibrosis,hepatitis B e antigen negative and γ-glutamyl transpeptidase at entry with relative risk ratio of 1.898,1.918,8.976 and 1.006,respectively.Conclusion HBV-DNA level is correlated with progress to cirrhosis in patients with chronic hepatitis B.

With depth understanding of the mechanism of human leukocyte antigen G (HLA-G) protein,more and more studies have found that HLA-G is closely related with tumor immune escape.Numerous studies have shown that the expression of HLA-G protein and mRNA could be detected in patients with cancer.

Objective To probe the neuroprotective mechanism of Donepezil in hippocampus of mice with vascular dementia(VD).Methods The mice were subjected for reperfusion repeatedly on bio-lateral common carotid arteries by repeatedly clipping and injected sodium nitroprusside into the abdominal cavity to establish the imitative VD rat models.Then Donepezil was given by intragastric administration.The behavioral abnormalities were investigated by Y-type maze test.The expressions of N-methy1-D-aspartate receptor subunit proteins(NR1)in neuron of hippocampus were observed by immunohistochemistry technique,and the activities of glutathione peroxidase(GSH-PX)and catalase(CAT)were measured with dithio-bis-nitrobenzoic acid(DTNB)and ultraviolet spectrophotometry(UV)methods,respectively.Results Compared with VD control group,the performance records of learning and memory in Donepezil group were better(all P

Liver Imaging Reporting and Data System was released online in 2011 by America College of Radiology (ACR) for standardizing the performance,interpretation and reporting of CT and MR imaging examinations of the liver in patients at risk for hepatocellular carcinoma.This article overviewed the profile of this system,its updated version and recent progress on its clinical application.