Long non-coding RNAs are important regulators of gene expression.ANRIL which was coded on the Chr9p21.3 loci participates in the pathogenesis of tumor, coronary artery disease, type 2 diabetes mellitus and oth-er diseases.Multiple ANRIL isoforms are tissue-specific.ANRIL mainly functions through Polycomb proteins, while there are also other downstream targets.The mechanism of each isoform and the downstream pathways are hotspots incurrent researches.

Objectives: To compare the efficacy of patient-controlled epidural analgesia and patient-controlled intravenous analgesia in postoperative analgesia after spinal fusion. Methods: The CNKI, Wanfang, CBM, Pubmed, Embase, Ovid, Cochrane library databases were searched by computer. The randomized controlled trails with patient-controlled epidural analgesia or patient-controlled intravenous analgesia after spinal fusion published from May 1985 to July 2013 were selected. All of them were assessed by the standard of Cochrane systematic review. Data of postoperative VAS and the rate of side effects after using the analgesic drugs were extracted into an electrical sheet, which was synthesized by a Meta-analysis with RevMan 5.2 software. Re-sult: Eight randomized controlled trails involving 482 patients met the inclusion criteria. And the quality grade of 3 literatures was A, 5 literatures was B. The result of meta-analysis showed that: ①Visual analogue score(VAS). The postoperative first day′s VAS[WMD=-0.47, 95%CI(-0.74, -0.20)], second day′s VAS[WMD=-0.66, 95%CI(-1.14, -0.19)] showed that the patient-controlled epidural analgesia had a good analgesic effect compared with the patient-controlled intravenous analgesia, and both had statistic differences(P<0.05). No dif-ference in the postoperative third day′s VAS[WMD=-0.58, 95%CI(-1.38, 0.21)] was observed between the two methods, and no statistic difference(P>0.05); ②The side effects. The patient-controlled epidural analgesia had a higher incidence of skin itch[RR=1.53, 95%CI(1.08, 2.16)], paresthesia[RR=3.34, 95%CI(1.12, 9.98)] after surgery than the patient-controlled intravenous analgesia, and both had statistic differences(P<0.05). While two groups had no a significant deviation about nausea [RR=1.05, 95%CI (0.79, 1.40)], vomiting [RR=0.80, 95%CI (0.48, 1.31)], and no statistic difference(P>0.05). Conclusions: The patient-controlled epidural analgesia has better analgesic effects on the postoperative first and second day after spinal fusion, but obviously has a high-er incidence of skin itch and paresthesia than the patient-controlled intravenous analgesia.

Objective To construct recombinant plasmid with p24 and gp41 gene, express fusion protein in E.coli. Methods To design primer with restriction endonuclease position,and amplify p24 and gp41 by RT-PCR, link both into pMD18-T vector.To choose correct clone with target gene.Then p24 fragment will be cleaved and linked into pMD18-T vector within gp41 gene. Both post-linked gene will be cleaved and linked into pET21a vector. The vector will be transformed into E.coli. And protein is highly effective expressed in E coli. Western blotting proved that the expressed product could react with 6?his antibody. Result Fusion protein p24-gp41 is highly effective expressed in E.coli. Conclusion Fusion protein p24-gp41 is highly effective expressed in E.coli in pET21a vector.

Objective:To explore the characteristics of consultation-liaison psychiatry service in general hospital.Methods:Totaly 541 consecutive inpatients from general hospitals were enrolled,who were referred to the consultation-liaison psychiatry service of a psychiatric hospital during a one-year period.The data was analyzed,including demographic characteristics,reasons for referral,referral department,psychiatric diagnosis according to criteria in the 4th edition of the Diagnostic and Statistical Manual of Mental Disorders(DSM-IV),and psychiatric management suggestions.Results:The rate of psychiatric consultation in the hospital which applied for the most consultations was 1.0%.The patients with psychiatric history(22.6%)were most likely to be referred.The principal reasons for psychiatric referral were psychotic episode(31.8%),depression(13.1%),and anxiety disorder(10.9%).According to DSM-IV,cognitive disorders(32.2%),depressive disorder(17.7%),anxiety disorder(13.1%),schizophrenia and other psychotic disorders(9.8%)were the major psychiatric diagnoses.The psychiatrists prescribed antipsychotics in 72.8% patients,gave nursing and behavior suggestions in 39.2% patients,gave following-up suggestion in 38.1% patients,and provided suicide prevention in 10.2% patients.Conclusion:It is important to expand the consultation-liaison psychiatry service in general hospital.And it is necessary to improve the physicians' ability in recognition and treatment of psychiatric disorder in patient with physical illness.

Objective To observe the effects of mild hypothermia on the dynamic change of neuron specific enolase (NES) in serum, the damaged neuron number around the impact site and the content of brain tissue water following traumatic brain injury (TBI) in rats so as to provide a quantitative parameters to evaluate the protective effect of the mild hypothermia on the brain. Methods A total of 45 SD rats were subjected to a lateral moderate cortical impact injury caused by a free-falling object and divided randomly into three groups:⑴ sham operation group (Group A);⑵ normothermic group (Group B);⑶ hypothermia group (Group C). The Group C was treated with mild hypothermia and the Group B with normothermia immediately after injury. A series of samples were obtained directly 3, 6 and 24 respectively after trauma in three groups. Pathomorphological method was used to observe the number of the damaged cortical neurons around the impact site. The serum concentration of NSE and the water content of brain tissue water were measured by radio-immunoassay. Results Following TBI in rats, the serum level of NSE was significantly increased (P

The activity of tPA and PAI of rabbits receiving different dosages of the Svate -3 were assayed with special substrata decomposition method. It was found that after Svate- 3 infusion, the activity of tPA increased but the activity of PAI decreased and it was related to the dosage and the speed of administration. The size of fibrinolysis in blood fibrin plate was in positive correlation to the activity of t-PA(r=0. 861,P

Objective To study the short term and long term response and the safety of the different doses of recombinant interferon alfa 1b(IFN? 1b) for the treatment of the patients with chronic hepatitis B and the predictive factors of the response. Methods 146 Chinese patients with chronic hepatitis B entered randomly into four groups. Three groups were given with IFN ? 1b 3 million unit (MU), 5 MU and 10 MU respectively. The control group was given placebo. The short term(ETR) and long term(SR 12) responses and side effect were observed. Selected nineteen items (host related, drugs related and virus related), which affected the response of IFN alfa 1b probably (host, virus and drugs, et al) were analyzed. Results The end treatment response (ETR) of the IFN ? 1b 5 MU therapy was 55.3%, and 10 MU was 58.1%. Both efficacy were higher than the group of 3 MU IFN alfa 1b (32.6%) and control therapy group (5.9%, P

Objective To measure normal range of CD4 from Chinese adults in Shanghai area by flow cytometry. Methods CD4 and CD8 value were measured through flow cytometry in adult blood specimens from various age and gender group. Results There were no difference in CD4 value among different sex and age groups, with the mean value being 726.99?255.21. However, CD8 and CD4/CD8 ratio vary significantly among different groups. The mean value of CD8 was 539.58?134.07, while the mean value of CD4/CD8 1.49?0.57. Conclusions The mean value of CD4 in Shanghai adults is about 100/mm 3 lower than that in American.

Theory of Shen-Xu Pang-Guang-Re (SXPGR) is an important part of the theory of zang-fu in traditional Chinese medicine (TCM). And it is the generalization for etiology and pathogenesis of Lin-Zheng (LZ). In immunology, mucosal immune system is composed of mucosal-associated lymphoid tissues and immune cells, which can resist the invasion of microorganisms. Mucosal immune plays an important role in the occurrence of urinary tract infection. This paper, which was based on SIgA and homing mechanism, was to investigate the relevance between mucosal immunity and the theory of SXPGR, in order to study the connotation of SXPGR in TCM.

Objectives To investigate the etiological factors,clinical features and treatment of pediatric primary spontaneous pneumothorax (PSP). Methods The clinical data of 84 PSP patients under the age of 18 from Departments of Emergency,Respiratory Diseases and Thoracic Surgery of our hospital from June 1999 to June 2009 were collected. There were 72 males (85.7%) and 12 females (15.3%) with a ratio of 6 to 1. Their age ranged from 14 to 18 (mean 17.06). Patients with pneumothorax following trauma,pneumonia,iatrogenic and other underlying causes were excluded. Therapeutic methods included oxygen therapy for 4.8% patients,closed drainage of pleural cavity for 57.1% patients,and surgery for 38.1% patients,But there were 31 patients receiving surgical treatment after closed drainage was failed. The period of follow-up ranged from 1 to 123 months (mean 25.73 months) through telephone or outpatient. Results Eighty-four patients were included,of whom 96.4% had chest pain,7.1% had chest tightness,66.7% had dyspnea,8.3% had shortness of breath and 14.3% had cough. There were 96.4% patients having decreased breathing sound and 3.6% having absent breathing sound. Bullae or blebs occurred mostly in apex and upper lobe. Twenty-one patients suffered from recurrent pneumothorax. Smokers (66.7%,n=9) had higher recurrent rate than non-smokers (22.0%,n=75). Conclusion PSP should be considered when symptoms such as chest pain and dyspnea appear in teenagers. Closed drainage of pleural cavity combined with surgical procedure is recommended for treatment. PSP patient with a history of smoking has higher recurrent rate of PSP.