This paper reports that in order to study the changes of lipoperoxidation rate in children with viral myocarditis, serum lipid peroxide (LPO), vitamin E (VE) concentration, and hydrogen peroxide hemolysis percentages of red blood cell (RBC H_2O_2 hemolysis %) were determined in 71 cases with viral myocarditis and it was found that LPO levels and RBC H_2O_2 hemolysis % in these patients were higher than in normal children(LPO levels in these patients were also higher than in the children with upper respiratory tract infection), LPO concentration of the patients presented light-level positive correlation to their RBC H_2O_2 hemolysis % (r=0.37, P

In clinical practice,we have adopted the methods such as intensifying the knowledge of preclinical medicine,learning to collect case history,training medical check-up,understanding the significance of auxiliary examination and encouraging the students to form the clinical thinking ability,and thus achieved satisfactory effect and obtained the positive opinion of students.

Sepsis is still a major cause of death among neonates and its morbidity rate remain high nowadays.Because of the atypical symptoms and extremely dangerous progress in neonatal sepsis,early diagnosis and treatment are requisite.But the unique biomarkers are lacking meanwhile.Recent study shows that PBEF influences individual susceptibility,severity and outcome in sepsis.This review synthesizes the research of PBEF in neonatal sepsis in order to provide the evidence of reliable biomarker to diagnose and treat neonatal sepsis in the early stage.

The integrated courses in pathophysiology have been implemented for clinical medicine in Nanfang Medical University with experimental contrast method since 2006 to compare the advantages of the teaching effect of the integrated courses with traditional learning in pathophysiology with the questionnaire. The results have showed that the students on the integrated track are more positive in the knowledge learning,problem analysis and solving as well as the subsequent clinical knowledge learning.

Objective To investigate the relationship between the C161T polymorphisms in peroxisome proliferators-activated receptor γ (PPARγ) gene and atherothrombotic infarction (ATI) in a Chinese Han population.Methods The patients with atherothrombotic infarction and controls,who without histories of stroke,transient ischemic attack and myocardial infarction were enrolled.Genotypes of the PPARγ C161T polymorphisms were determined using the polymerase chain reaction-restriction fragment length polymorphism.The frequencies of genotype and allele in patients with ATI and controls were compared.Results A total of 112 ATI patients and 112 controls were enrolled.There were no significant differences in the age (67.9± 11.73 years vs.66.5 ± 10.35 years; t =0.386,P =0.701) and the proportion of male (61.61％ vs.58.04％ ;x2 =0.297,P =0.586) in the ATI group and the control group.The proportions of hypertension (59.82％ vs.44.62％ ;x2 =5.171,P =0.023),type 2 diabetes mellitus (26.79％ vs.9.82％;x2 =10.778,P =0.001) in the ATI group were significantly higher than those in the control group.Body mass index (25.13±1.86 kg/m2 vs.24.11 ± 1.81 kg/m2; t =3.543,P=0.001),systolic pressure (158.84 ± 20.15 mm Hg vs.135.82 ± 19.58 mm Hg; t =7.350,P =0.000),diastolic pressure (76.90 ± 13.64 mm Hg vs.68.90 ± 8.52 mm Hg; t =4.374,P =0.000) and the level of fasting blood glucose (6.523 ± 2.831 mmol/L vs.5.706 ± 2.177 mmol/L; t =2.026,P =0.044) in the ATI group were significantly higher than those in the control group.The genotype frequencies of CC,CT and TT in the ATI group were 77.7％,17.0％ and 5.4％,respectively,and in the control group were 69.6％,22.3％ and 8.0％,respectively.There were no significant difference between the two groups (x2 =1.909,P =0.385).The allele frequencies of C and T in the ATI group were 86.2％ and 13.8％,respectively,and in the control group were 80.8％ and 19.2％,respectively.There were no significant difference between the two groups (x2 =2.331,P =0.127).Conclusions PPARγ C161T polymorphisms may be not associated with the onset of ATI in a Chinese Han population.

OBJECTIVE:To investigate the cognition of clinical pharmacists on the clinical pharmacy practice and their profes-sion,and provide reference for further developing clinical pharmacy practice. METHODS:A questionnaire was designed,and ran-dom sample was conducted for the clinical pharmacists from 30 secondary and tertiary hospitals in Shanghai,and the investigation results were analyzed statistically. RESULTS:Totally 130 questionnaires were sent out,and 102 were effectively received with valid response rate of 78.46%. 94.11% of the investigated subjects were willing to be a clinical pharmacist,but 17.65% of them consid-ered that they were incompetent for their work;29.41% of them thought the current situation of clinical pharmacy practice was not ideal;all the respondents considered that the clinical pharmacist system should be implemented in medical and health institu-tions;the cognition and evaluation of investigated subjects in tertiary hospitals on clinical pharmacy practice and their profession was generally higher than those in secondary hospitals(P<0.05). CONCLUSIONS:The clinical pharmacists in medical and health institutions in Shanghai showed high cognition on their profession,and the clinical pharmacy practice has already made some achievements,but many work still need to be improved. Therefore,clinical pharmacists should continuously study their profession-al knowledge and improve their professional skills and quality;hospitals and universities should strengthen the subject construction and personnel training of clinical pharmacy and deeply carry out the clinical pharmacist system;health administration departments should strengthen the related laws and regulations of clinical pharmacy;colleges and universities should strengthen the discipline construction and personnel training.

Objective To study the feasibility and reliability of median nerve somatosensory evoked potential monitoring in neurosurgery around the brain stem. Methods in selected 43 patients SEP N20, waveforms were recorded after stimulation of left and right median nerves respectively. Anesthesia was maintained with intravenous anesthetics throughout the operation. As soon as N20 waveform changed significantly, the surgeon was informed and the operative procedure was modified whenever possible. Results SEP waveforms were almost stable during maintaining with total intravenous anesthesia (TIVA). Within 43 cases, SEP waveform of & patients significantly changed. In these cases, SEP of 5 patients returned to normal gradually and no further neurologic dysfunction happened postoperatively. The other 3 cases did not recover, and postoperative neurologic complications happened. There was no false-negative or false-positive result. The correlation between intraoperative SEP changes and postoperative neurologic alterations was significant statistically. Conclusions SEP recording is a reliable technique for intraoperative monitoring during neurosurgery around the brain stem. TIVA is helpful for SEP monitoring.

Objective To probe the clinical character,the histopathological classification and misdiagnoses of intraocular tumors. Methods The clinical and pathological data of 359 patients with intraocular tumor diagnosed clinically between 1980～2000 were retrospectively analyzed. Results There were 300 cases of malignant tumor and 23 cases of benign tumor respectively. Non oncologic malady and benign tumor misdiagnosed as tumor or malignant tumor were 40 cases. The two leading malignant tumors were retinoblastoma and melanoma. Conclusion The clinical and pathological analysis of intraocular tumor is beneficial to the correct clinical diagnosis and treatment.

0.05) . There was no significant difference in Q-T interval and Q-Tc before and after intravenous droperidol in the three groups. Conclusion It is safe to give low dose droperidol before operation to prevent postoperative nausea and vomiting.

AIM: To identify the key factors responsible for drug resistance in different ovarian cancer cell lines using protein microarray system. METHODS: Six ovarian cancer cell lines were employed. The sensitivity of ovarian cancer cell line to common chemotherapeutic drugs was determined by using MTT assays. The expression of 78 cytokines and other factors was examined by using cytokine antibody array technology. RESULTS: Different ovarian cancer cell line responded to chemotherapeutic agents differently. The drug resistance was correlated with certain cytokine expression. Cell line SKOV3 was less sensitive to first line chemotherapeutic drug (ADM, CBPDA) and accumulated high amounts of GRO and TIMP-2 compared with other 5 cell lines. OVCAR4 cells were more resistant to second line chemotherapeutic drug (TAXOL, VP16) and had higher levers of IL-6 and IL-8 than IGROV1, OVCAR3 and OVCAR5. CONCLUSIONS: Among the most common excretive cytokines, increasing of GRO, IL-6, IL-8 and TIMP-2 might be related to drug-resistance of ADM and CBPDA in ovarian cancer cell, while IL-6 and IL-8 might also be related with drug resistance of TAXOL and VP16. The different types of ovarian cancer cell might have roughly similar excretive cytokines-induced mechanism of drug resistance.