Objective To explore the effect of chlamydia pneumoniae(CP)infection on T lymphocyte subsets in patients with chronic obstructive pulmonary disease(COPD).Methods A total of 176 patients with acute exacerbations of COPD who were admitted to hospital from Jan.2001 to Jan.2005,and 46 healthy subjects were selected.Chlamydia pneumoniae-specific serum IgG,IgA,IgM antibodies were measured by microimmunofluorescence(MIF)test;the flow cytometer was used to analyze the frequency of CD+_3,CD+_4 and CD+_8 lymphocytes in patients with COPD and controls.Results The rate of acute chlamydia pneumoniae infection in COPD patients with acute exacerbations was 27.3% and that of chronic CP infection was 19.3%.They were all significantly higher than that in healthy subjects(P﹤0.01).The frequency of the CD+_3 and CD+_4 lymphocytes showed no difference in each group.The frequency of CD+_8 lymphocyte in COPD patients of chronic CP infection rose and the CD+_4/CD+_8 ratio was decreased in comparison with controls.On the other hand,the CD+_8 lymphocytes and the CD+_4/CD+_8 ratio showed no difference between chronic CP infection group,no CP infection group and controls.Conclusion The chlamydia pneumoniae infection is a rather frequent event in acute exacerbations of COPD,and it is an important reason to cause the disorder of cell-mediated immunity in COPD patients.The disorder of cell-mediated immunity may be involved in pathogenesis of COPD.

Objective To study the radiographic findings of anomalies of thoracic skeletion in patients with congenital heart disease.Methods Frontal and lateral chest films of 252 cases with congenital heart disease proved by operation were reviewed.Results The skeletal anomalies in 8 cases including generalized sternal prominence,sternal bowing,pouter pigeon breast,hemivertebrae and butterfly vertebrae of thoracic spine,and deformities of ribs were discovered.Conclusion The skeleton anomalies which are divided into primary and secondary types often occur in patients with congenital heart disease.

AIM: To identify the key factors responsible for drug resistance in different ovarian cancer cell lines using protein microarray system. METHODS: Six ovarian cancer cell lines were employed. The sensitivity of ovarian cancer cell line to common chemotherapeutic drugs was determined by using MTT assays. The expression of 78 cytokines and other factors was examined by using cytokine antibody array technology. RESULTS: Different ovarian cancer cell line responded to chemotherapeutic agents differently. The drug resistance was correlated with certain cytokine expression. Cell line SKOV3 was less sensitive to first line chemotherapeutic drug (ADM, CBPDA) and accumulated high amounts of GRO and TIMP-2 compared with other 5 cell lines. OVCAR4 cells were more resistant to second line chemotherapeutic drug (TAXOL, VP16) and had higher levers of IL-6 and IL-8 than IGROV1, OVCAR3 and OVCAR5. CONCLUSIONS: Among the most common excretive cytokines, increasing of GRO, IL-6, IL-8 and TIMP-2 might be related to drug-resistance of ADM and CBPDA in ovarian cancer cell, while IL-6 and IL-8 might also be related with drug resistance of TAXOL and VP16. The different types of ovarian cancer cell might have roughly similar excretive cytokines-induced mechanism of drug resistance.

Objective:To study the effects of anti-oncogene PTEN transfection on apoptosis of human bladder cancer cell lines,BIU-87,and to bring a new method of gene therapy for bladder cancer. Methods:A eukaryotic expression vector containing PTEN was transfected into BIU-87,and positive cell clones were selected and amplified.Expression of PTEN was detected by RT-PCR.Apoptosis of BIU-87 were measured before and after transfection by in situ cell apoptosis detection kit and flow cytometry. Results:PTEN transfected BIU-87 could steadily express PTEN mRNA,and cell apoptosis significantly increased compared with control groups. Conclusion:Transfection of PTEN might induce apoptosis of bladder cancer cells so as to inhibit the occurrence and development of tumor.

Variants of gene loci on susceptibility genes are the major individual susceptibility factors of esophageal squamous cell carcinoma (ESCC) in China.Some of the gene loci participate in the DNA damage and repair,and some are related with cancer suppressor genes,metabolism enzymes,trace elements and smoking.The single nucleotide polymorphisms of these susceptibility genes are closely correlated with the genesis of ESCC.

The external treatmen of traditional Chinese medicine, the enema and the acupuncture is the current methods of external treatment of traditional Chinese medicine treatment for cirrhosis ascites. In the field of external application and enema, most literature reported specific herbal formular for specific disease, while little literature reported combination of oral administration of herb and external application, or external application based on TCM pattern differentiation, or external application on the acupoints. Acupuncture can reduce drug use and avoid their damage on the liver. This paper summarized the current researches of Chinese medicine external treatment, and points out the direction of the research on the external treatment of traditional Chinese medicine.

This study examined the differences in tumor formation of three bladder tumor cell lines (BIU-87, T24 and EJ) after subcutaneously transplanted into nude mice, in order to find the best technique for establishing in vivo bladder tumor model. BIU-87, T24 and EJ cells at logarithmic phase were re-suspended in serum-free medium. The cells suspensions of the identical concentration were subcutaneously transplanted into nude mice and then the success rate and tumor growth were compared among the three cell groups. The results of tumor formation were pathologically evaluated. Lung, liver and kidney tissues were also pathologically examined for distant metastasis. The proliferation of the three cells were determined by immunohistochemically detecting the PCNA expression in the tumors. The results showed that the success rates of EJ and T24 cells were significantly higher than that of BIU-87 cells and no distant metastasis was noted among the three groups. The proliferation levels of EJ and T24 cells was significantly higher than that of BIU-87. But at the later stage of tumor formation, as compared with T24 cells, EJ grew more vigorously, soon resulting in the central necrosis of tumor, which affected the measurement of the actual size of the tumors. Moreover, PCNA staining exhibited that the proliferation of EJ and T24 was significantly higher than that of BIU-87 cells. It is concluded that as compared with BIU-87 cells, EJ and T24 cells had higher success rates, with not significant differences in death rate and distant metastasis found among them. There existed no significant difference in tumor formation between EJ and T24 cells and T24 cells do not rupture easily, which makes it a better cell line for the establishment of in vivo bladder tumor model.

Objective To probe the neuroprotective mechanism of Donepezil in hippocampus of mice with vascular dementia(VD).Methods The mice were subjected for reperfusion repeatedly on bio-lateral common carotid arteries by repeatedly clipping and injected sodium nitroprusside into the abdominal cavity to establish the imitative VD rat models.Then Donepezil was given by intragastric administration.The behavioral abnormalities were investigated by Y-type maze test.The expressions of N-methy1-D-aspartate receptor subunit proteins(NR1)in neuron of hippocampus were observed by immunohistochemistry technique,and the activities of glutathione peroxidase(GSH-PX)and catalase(CAT)were measured with dithio-bis-nitrobenzoic acid(DTNB)and ultraviolet spectrophotometry(UV)methods,respectively.Results Compared with VD control group,the performance records of learning and memory in Donepezil group were better(all P

Objective To study the relationship of expr ession of PTEN and vascular endothelial growth factor (VEGF) with angiogenesis i n bladder cancers. Methods Using immunohistochemical S- P method,the expression of PTEN and VEGF was examined in 62 bladder cancer sampl es and 18 chronic cystitis samples (control group);and the microvessel density ( MVD) was compared among different expression states of PTEN and VEGF in bladder cancer tissues. Results The positive rates of PTEN in bl adder cancer and control groups were 53.2% (33/62) and 100.0% (18/18),respective ly.The difference between the 2 groups was significant ( P

Objective To observe the neuroprotective role and its mechanism of estrogens in male Sprague-Dawley rats with focal cerebral ischemia. Methods The rats were randomly divided into three groups: E2-pretreat group, E2+TAM (tamoxifen)-pretreat group and control group. The models of cerebral ischemia were maded by occlusion middle cerebral artery using an intraluminal filament method. 2 and 24 hours after focal cerebral ischemia, the neurological deficit scores and the death rate of rats were evaluated. 2,3,5-triphenyltetrazolium chloride (TTC) stain was used to assess the volume of infarction. Terminal transferase dUTP nick ending labeling (TUNEL) and immunohistochemistry were carried to observe neuron apoptosis and the expressions of Bcl-2, Bax and p53. Results Compared with control group, the volumes of infarction, death rates, neurological deficit scores and the numbers of neuron apoptosis both at 2 and 24 h were significantly decreased in E2-pretreated group (all (P