Objective:To formulate the standardized nursing process for ultrasound guided joint cavity puncture based on evidence-based medicine, and explore its effect.Methods:In January 2021, the standardized nursing process for ultrasound guided joint cavity puncture in orthopedic based on evidence-based medicine was established. From July 2020 to August 2021, 200 outpatients with ultrasonic guided joint cavity puncture in orthopedic were selected from the Shanghai Sixth People's Hospital Affiliated to Shanghai JiaoTong University by convenience sampling. The patients before the implementation of the standardized process were taken as the control group, and the patients after the implementation of the standardized process were taken as the observation group, with 100 cases each. We compared the operation score difference of ultrasonic guided joint cavity puncture and the operation time of joint cavity puncture among nurses, as well as the pain, swelling and puncture-associated adverse events of the two groups among patients before and after the implementation of the standardized process.Results:After the implementation of the standardized process, the scores of nurses in the 5 aspects of pre operation preparation, operation process, proficiency score, theoretical total score and total score of the joint cavity puncture operation were higher than those before the implementation, and the differences were statistically significant ( P<0.05) . The operation time of joint cavity puncture in the observation group was shorter than that in the control group, with a statistically significant difference ( P<0.05) . The swelling score, pain degree and incidence of puncture-associated adverse events in the observation group 24 hours after operation were lower than those in the control group, with statistically significant differences ( P<0.05) . Conclusions:The standardized nursing process for ultrasound guided joint cavity puncture in orthopedic based on evidence-based medicine can help nurses improve the operation theory and proficiency, shorten the operation time during the operation, reduce the swelling and pain of patients after the operation, and avoid the occurrence of puncture-associated adverse events.