Objective To evaluate the diagnostic value of multi-slice spiral CT urography(MSCTU)in pyelic and uretal disease.Methods 46 cases of pyelic and ureter disease with integrated clinical data were collected in two years,all cases were undergone multi-slice spiral CT contrast-enhanced scanning,the excretory-phase images were transferred to the workstation and shaded volume rendering(SVR),maximun intensity projection(MIP),multiplanar reconstruction(MPR)and curved planar reconstruction(CPR)were performed.Results In 46 cases,16 pyelic and ureteral calculus,12 congenital deformitions(including pelviureteric duplication malformations,retrocaval ureters,ureteropelvic junction stenosis,supern-umerary kidneys),5 inflammatory stenosis,7 primary pyelic and ureteral cancer,4 ureters were involved by bladder cancer and 2 compressive lesion were found.Conclusion MSCTU has obvious advantage in the diagnosis of pyelic and uretal disease.

Influenza,caused by influenza virus,is a respiratory infectious disease with a serious hazard to human health.Prevention of influenza through vaccine development is the most effective strategy.It is important to build a rapid response platform for research and production of influenza vaccine.As virus vectors,live vaccine provides a new prevention and treatment way for infectious disease.Modified vaccinia virus Ankara(MVA) is a replication-deficient viral vector that is safe and can encode one or more foreign antigens and induce humoral and cellular immune response.MVA holds great promise as a vaccine platform.In this review,we discuss the use of MVA for vaccine development against influenza virus.

Objective To estimate the ability of MSCTA in displaying internal mammary artery . Methods MSCTA images of clinically suspected aortic disease in 30 patients were retrospectively re-processed and analyzed.The transverse images were combined with the reorganization images to observe the running rule,length and diameter of internal mammary artery.Results 60 internal mammary arteriae were showed by MSCTA,one was maldevelopment and obvious smaller than that of the contralateral one,four in two patients were obvious circuitous and two in one patient appeared abnormal branch.All internal mammary arteries originated from the outside of the first segmental of the left and right subclavian artery respectively,and descended along the back of the upper 6 sostal cartilages,in the distance of 2 cm to the limbus of sternal border,then divided into two in the sixth intercostal space level.56 on anterior view showed straight line descent,while on lateral view,they closed to the costal cartilage and were mild-circuitous.The average length of internal thoracic arteries was:(20.11?1.85) cm in right and (20.34?1.57) cm in left, the average diameter was:(2.49?0.37) cm in right and (2.45?0.35) cm in left.Conclusion MSCTA can clearly demonstrate the internal mammary artery,which provides various kinds of information for the clinical diagnosis and treatment of certain diseases.

Objective To estimate the value of MSCTA in the diagnosis of subclavian arterial stenosis.Methods 10 patients with suspected subclavian steal syndrome which causes subclavian artery stenosis underwent MSCTA, the contrast medium was Iohexol 80~90 ml, the flow rate was 3~4 ml/s with sure-star techniques,excretory-phase images were transferred to the workstation and shaded volume rendering (VR),multiplanar reconstruction(MPR),and curved planar reconstruction (CPR) were performed.The left subclavian artery was divided into proximal and distals by openning of left vertebral arteries. Results The narrowing positions:6 cases were in proximal and 4 cases were in distal of subclavian artery.6 cases were focal stenosis,4 cases were multiple stenosis and totally 14 focus.The extent of stenosis was 0.8~2.0 cm,10 cases were ≤1 cm and 4 cases were 1.1~20 cm.The degree of stenosis included 7 cases ≤50%,3 cases in 50%~70%.The results was same comparing with that of DSA.Conclusion MSCTA plays an important role in diagnosing subclavian arterial stenosis.

Objective To understand the clinical characteristics of systemic lupus erythematosus with Antiphospholipid syndrome (SLE-APS).Methods The clinical data of 39 cases of SLE-APS were collected and analyzed retrospectively. Results Thirty-one patients had 48 thrombosis episodes in total, among which the most common presenting manifestations were deep venous thrombosis and stroke. 12 of 26 married women had fetal morbidity. The prevalence of anticardio lipin antibody (aCL) and lupus anticoagulant ( LA) was 72% and 41% , respectively. 24 patients had SLE at first,on an average of 9. 5 years later they had the characteristics of APS, 12 patients had thrombosis episodes or fetal morbidities ,4. 8 years later they developed into SLE,and 3 patients had the characteristics of SLE and APS from the onset The SLEDAI was less than 5 in five cases when they had thrombosis episodes or fetal morbidities.Conclusions The incidence of thrombosis episodes or fetal morbidities increased in SLE-APS patients. APS occurred before, after or at the same time with SLE. SLE patients might have the clinical features of APS during the steady stage. It's very important to ask the medical history in detail and measure aCL in lupus patients to find the risk factor of APS and prevent the APS' occurrence.

Objective To evaluate the value of multi-slice spiral CT(MSCT) post-processing technique in diagnosis of spinal injury. Methods CT materials in 44 cases of spinal injury were retrospectively analyzed,all case were scanning with 16-slice helical CT,the images were passing on an independent workstation for MPR and SVR technique,and compared with axial 2D imaging of CT . Results MPR and SVR images could show the fracture line and displacement of fractured osseous fragments and spatial relationship of structure clearly,MPR images could show small fracture line and dislocation of joint actually. Conclusion MPR and SVR images are important supplement to axial 2D images,the axial imaging combined with various reconstruction technique can provide the space structure of spinal injury ,and play an important role in selecting therapy project and evaluating prognosis.

Objective To evaluate the value of multi-slice spiral CT(MSCT)post-processing technique in diagnosis of small peripheral lung cancer .Methods CT materials with small peripheral lung cancer in 20 cases were retrospectively analyzed ,after 16-slice spiral CT scanning ,both plain and contrast ,and reconstructed using 1mm slice thickness ,the images were passing on an independent workstation for SSD and MPR technique .Results the images of SSD and MPR could reveal the relation between focus and surrounding organs in different dirctions,also revealed the features of pulmonary carcinoma stereos copically and directly,could show actually lobulated sign ,burr sign,pleural indentation sign and vessel convergence sign with small peripheral lung cancer ,the SSD imaging was superior in judging the presence of the lobulated sign .Conclusion It is very valuable for diagnosis of small peripheral lung cancer that application of SSD,MPR and two dimensional reconstruction。

Objective To develop an analyzing technique of single nucleotide polymorphism(SNP) based on non-probe real-time quantitative PCR, and to explore the relationship between SNP in human adiponectin (APM1) gene and type 2 diabetes (T2DM).Methods Design primers with specific 3′-terminal nucleotides and perform Real-time PCR using SYBR Green dye. Genotypes were determined by comparing the amplification efficiency of each pair of primer and verified by sequencing. SNPs 45 and 276 in APM1 of 20 T2DM, 24 obesity and 28 healthy persons were analyzed. Results This technique could discriminate genotype as efficient as sequencing. The genotype of APM1 at +45 site was significantly correlated with T2DM (P

Objective To investigate the variation of vascular cell adhesion molecule-1 (VCAM-1) expression and lipid peroxidation in aorta and plasma of Ins2~ Akita diabetic mice, and to inquire into the relationship between the variation and the cardiovascular diseases in diabetic patients. Methods With the aortic and plasma samples from Ins2Akita diabetic mice, the protein expression levels of VCAM-1 were detected by Western Blot or ELISA, and the levels of lipid peroxides were determined by lipid peroxidation kit. Results Compared with that of normal control, VCAM-1 protein and lipid peroxides levels were significantly elevated in the aorta and plasma of Ins2~ Akita diabetic mice (P

In this article,we discussed the limitations of present clinical teaching situation, which included curriculum concept, clinical practice condition and quality of teachers. Based on the analysis of present education,we should constitute effective teaching mode,set curriculum system reasonably and take effective teaching means in the clinical teaching of laboratory medicine. It’s very important for educate students with practice talent and high integrative quality.