neoplasia.

70%). Imipenem was of the strongest activity against Pseudomonas aeruginosa with the susceptibility of 86.3% but cefetaxime and ceftriaxone were of the weakest with the susceptibility of 6.8% and 11.0%, respectively. Conclusion The clinically isolated drug resistant Pseudomonas aeruginosa strains are increasing year by year. Therefore, clinical doctors should choose antibiotics rationally in dealing with the P. aeruginosa infected patients according to drug resistance test.

Psoriasis is a recrudescent chronic inflammatory dermatosis which is called“tinea”in ancient times. Its pathogenesis is not only associated with changes in the blood and body fluids, but also has close connection with external infection of six evil factors. This article sorted data of pathogenesis and treatment from the perspective of external infection of six evil factors, and discovered that pathogenesis of psoriasis includes external factors of four evils of wind, cold, damp and heat and internal factors of blood deficiency and blood dryness. Ancient treatment was based on dispelling wind, clearing away heat and moistening dryness. The pathogenesis can be summarized as follows:wind and poison attack skin and hinder blood production. The treatment can be “let in air for detoxification, enrich blood and moister dryness, cooling and activating blood”, blood and functional status of organs of patients should be observed, with a purpose to provide references and basis for modern clinical prevention and treatment of psoriasis.

The terahertz laboratory medicine (THz-LabMed) is an intergrated cross-frontier field which involves multi-disciplinary including medicine,biology,biomedical engineering,physics,optics,computer science,information and materials.Using THz technologies for label-free detection and analysis of biomedical macromolecules,cell and tissues,the THz-LabMed is also the core components of terahertz biomedine (THz-BioMed) which focuses on the biomedical application of THz technologies.THz label-free detection is being paid more and more attention because of its unique advantages worldwide and becomes a hot spot for the application of THz wave technology and methods in life science.Application of THz technology in biomedicine involves many fields globally,including disease diagnosis,recognition of protein status,label-free DNA sequencing,mechanism for absorption differences of biological tissue to THz wave,and radiation influence on biological samples and biological process.THz-LabMed is the global synchronized research in THz-BioMed field in China.It is important to seize the opportunities to develop new disciplines of laboratory medicine.

Supported by Center of Laboratory Medicine of PLA,the Third Military Medical University launched teaching reform on laboratory diagnostics by integrating and optimizing teaching contents,adopting case centered teaching method,introducing thinking modes of evidence-based laboratory medicine and strengthening bilingual teaching.Abilities of students to determine the clinical stage and to evaluate the effect of treatment as well as prognosis of diseases were promoted.

Objective To explore the presence of common genetic alterations in retinoblastoma and to localize the altered genomic regions. Methods Genetic instability of chromosome 19, 20, 21, 22 and X of 15 microdissected retinoblastoma tumors were analyzed by the loss of heterozygosity (LOH) and microsatellite instability (MSI). Results Among the 15 patients with retinoblastoma, genome instability [LOH and(or) MSI] at one or more loci on the 5 chromosomes in 10 (67%), in which the loss of a single allele was more frequent in chromosomes 19 (33%) and 20 (27%) than in the other 3 chromosomes. High-frequency LOH between D19S902 and D19S571 suggested gene loci in the 19q13 region might be associated with tumor development in retina. According to the result of MSI, MSI occured at least in one subset of retinoblastoma. Conclusions Our results provide first evidence of LOH in chromosomes 19 and 20 in retinoblastoma and further support the presence of genome instability in retinoblastoma that may play an important role in the tumorigenesis or progression of retinoblastoma.

OBJECTIVE To explore the changes in foot fungus mobility and its drug resistance for further etiology investigation and clinical treatment. METHODS Sabourand′s agar culture medium was used to culture fungi, ID identification strip was employed to identify the fungi and drug sensitive test was performed by disk diffusion test. RESULTS The incidence of Trichophyton rubrum infection was the highest (79.1%). The isolated fungi were relatively sensitive to amphotericin B (AMB, 98.9%) and itraconazole (ITC, 98.0%), and resistant to 5-fluorocytosine (5-FC, 22.1%). CONCLUSIONS Detection technique of fungal infection should be improved and anti-fungal medicine should be used reasonably according to the results of drug sensitive test so that the fungal infection, especially fungi-resistant infection could be reduced.

OBJECTIVE A new piezoelectric aptamer biosensor is developed for determination of IgE. The energy converters are 10MHz AT-cut quartz crystals with gold-coated electrodes. The anti-IgE aptamers are immobilized onto the surfaces of crystals gold electrodes by biotin-avidin method. METHODS The standard substance and serum were detected to find the limit of detection and specificity of the biosensor. RESULTS The piezoelectric immunoglobulin aptamer biosensor could complete the detection without nonspecific response. Under the optimized conditions, the experimental results showed that the piezoelectric biosensor had good response to IgE whose frequency shifts were linearly dependent on IgE concentration in different range. The piezoelectric aptamer biosensor had been used to detect IgE in serum, the analytical results given by this method were in satisfactory agreement with those given by chemoluminescence method, its correlation coefficient was 0.9924. CONCLUSIONS Piezoelectric aptamer biosensor for the determination of IgE is of high sensitivity, high specificity, high analysis speed, unnecessary labeling, simple operation, real-time detection, etc. It is suitable for detection of IgE and should be used for clinical detection.

OBJECTIVE To monitor the characteristics of distribution and drug resistance of Acinetobacter baumannii in our hospital. METHODS A. baumannii isolates were collected in our hospital from Jan 2004 to Dec 2005. Antimicrobial susceptibility testing was performed by disk-diffusion method,according to the standards of NCCLS 2004. RESULTS Totally 177 strains of A. baumannii were distributed clinically in the respiratory unit as the most ones (47 strains, 26.6%), and in ICU as the next (38 strains, 21.5%); the older the age, the higher the appearing rate; the highest appearing rate was from the sputum, up to 78.1%; more than 60% of isolates were resistant to all antimicrobial agents tested except imipenem, meropenem and cefoperazone/sulbactam. However,10 pan-resistant strains were found. CONCLUSIONS With the increasing isolation rate of A. baumannii, its drug resistance increases simultaneously.

OBJECTIVE To establishan immuno-PCR assay with the carriers of gold-magnetic particles for detection of HIV-1 p24. METHODS The feasibility of using gold-magnetic particles as the carriers was verified. The gold-magnetic particles were coated with mouse anti-p24 monoclonal antibody as the capture antibody. The reporter DNA was initially generated by PCR amplification using a biotinylated primer, and was bound through streptavidin to biotinylated polyclonal antibody as the detection antibody. HIV-1 p24 sandwiched by two antibodies was detected by amplifying the reporter DNA using PCR. RESULTS The efficiency of gold-magnetic particles coated with mouse anti-p24 monoclonal antibody could reach up to 95%. Furthermore, the amount of antibodies immobilization was consistent among different batches of gold-magnetic particles and there was nearly without nonspecific adsorption. The detection limit of immuno-PCR assay was 0.1 ng/L, an approximately 1.5?104-fold higher compared with an enzyme-linked immunosorbent assay. The linear range of p24 concentration was 0.1-100 ng/L. CONCLUSIONS Gold-magnetic particle is one of the ideal immuno-PCR reaction carriers. The immuno-PCR for detection of HIV-1 p24 reported in this article is indicated to be a promising detection method.