Objective:To investigate the promoting effects of interleukin-13(IL-13)on the proliferation of airway smooth muscle cells(ASMC)in mice and its mechanism.Methods:Murine ASMC were isolated and subcultured.IL-13,IL-13plus AG1478,IL-13plus neutralizing antibody to TGF-?,IL-13plus neutralizing antibody to EGF,IL-13plus neutralizing antibody to HB-EGF,IL-13plus dexamethasone were added to the media,respectively.MTT and 3 H-TdR incorporation assays were used.Apical media were analyzed for the presence of soluble TGF-?using ELISA.Results:The D 595 value of MTT assay and the cpm value of 3 H-TdR incorporation assay of IL-13group were significantly higher than those of control group(P

Objective To investigate the value of CT in the diagnosis of dedifferentiated liposarcoma.Methods Fifteen surgical operations of dedifferentiated liposarcoma in 11 patients, confirmed by pathology, underwent plain (n=15) and contrast enhanced (n=12) CT scans. Two of the 12 lesions underwent dynamic multi-phase contrast scans. The CT features and the corresponding pathological basis were analyzed.Results The lesions were divided into 3 groups according to the fatty component and its distribution. The CT appearances were as follows: (1)a well delineated fatty component in the lesion and a closely apposed non-fatty region in 10 cases including 5 initial (5/5, 100%) and 5 recurrent (5/10, 50%), which depicted an abrupt transition from the well-differentiated area to the high-grade sarcoma component under microscope; (2)fatty component within the lesion was less than 10% in 3 recurrent (3/10, 30%); (3)fatty masses and non-fatty masses separately distributed in 2 recurrent cases (2/10, 20%). Contrast enhanced CT scan showed moderate to marked inhomogeneous enhancement in the early phase in all 12 lesions, and delay enhancement was also observed.Conclusion Dedifferentiated liposarcoma exhibited specific appearances as well delineated fatty component and closely apposed non-fatty region with apparent enhancement. Dynamic contrast CT scans play important roles in the diagnosis and differential diagnosis of dedifferentiated liposarcoma, and help pre-surgical grading and pathological sampling.

Objective To explore the correlation between cardiovascular risk factors and characteristics of coronary artery le‐sions ,the degree of stenosis in patients with coronary artery disease(CAD) .Methods Totally 156 patients who under went coro‐nary angiography(CAG) in our hospital were divided into CAD group(n=98) and non‐CAD group(n=58) which the patients with coronary stenosis <50% according to the result of CAG .Their clinical data were retrospectively analyzed ,including gender ,age , body mass index(BMI) ,family history ,smoking status ,left ventricular ejection fraction(LVEF) ,blood lipid ,blood pressure and blood sugar levels ,the correlation between cardiovascular risk factors and coronary artery lesions was analyzed .Results There was significant difference of gender ,systolic blood pressure (SBP) ,total cholesterol (TCH) ,three acyl glycerin (TG) ,low density lipo‐protein cholesterol (LDL‐C) ,fasting blood glucose (FBG) ,smoking between the two groups(P<0 .05) ,but no significant differ‐ence was found at the level of ages ,BMI ,high‐density lipoprotein cholesterol (HDL‐C) ,diastolic blood pressure (DBP) ,family his‐tory ,serum creatinine (Cr) ,LVEF between the two groups(P>0 .05) .The number of coronary artery lesion branch were increased with these risk factors incresed ,such as smoking ,SBP ,LDL‐C ,FBG ,no significant difference with age ,male ,TCH ,TG .The degree of stenosis were increased with SBP ,FBG ,no significant difference with age ,male ,smoking ,TCH ,TG and LDL‐C .Conclusion The smoking ,males ,FBP and SBP are the prominent risk factors of CAD in Qinghai area ,prosmote the occurrence and development of CAD together .

Objective To investigate the effectiveness and safety of minimal invasive surgery combined with ventriculoscope for the treatment of basal ganglia hemorrhage.Methods From June 2014 to June 2015 there were 57 patients with basal ganglia hemorrhage were selected into this study.These patients were signed into the minimal invasive surgery combined with ventriculoscope group and the craniotomy group according to the methods of surgery they received.And the clinical outcomes of the two groups were compared.Results Compared with the craniotomy group,patients in the minimal invasive surgery combined with ventriculoscope group got a significantly lower Glasgow coma scale at 7 days,14 days and 28 days after the operation (P =0.02,0.04,0.04);the hospital stays were significantly reduced in the minimal invasive surgery combined with ventriculoscope group [(21.45 ±5.67)d vs.(25.67 ±7.45)d,P =0.02];and the operation time were significantly reduced as well [(134.45 ±21.11)min vs.(178.65 ±45.32)min,P =0.000)].There was no significant difference in intra-cranial pressure,rate of hematoma clearance,rate of organ functional failure,rate of re-bleeding and mortality 28 days after operation (P >0.05).Conclusion The minimal invasive surgery combined with ventriculoscope is effective and safe for the treatment of basal ganglia hemorrhage,which is worthy of popularization.

Objective To study the changes in cortical activation of frontal lobes and temporal lobes between schizophrenic patients and comparison subjects during a verbal fluency task by using functional magnetic resonance imaging (fMRI). Methods Thirteen schizophrenic patients and 8 volunteers were studied during a verbal fluency task performance. fMRI of frontal lobes and temporal lobes was conducted at a GE 1.5 T Signa Horizon LX scanner while the subjects performed the task. Stimulus sequences were divided into six 30-second segments by using a task-activation paradigm that alternated between resting and stimulated states. Results Schizophrenic subjects demonstrated significantly less left frontal activation than comparison subjects during a word fluency task, and comparison subjects showed evidently more activation in left frontal lobes than in the right (P0.05). Conclusion These preliminary data suggest that fMRI has the sensitivity to detect the differences in activation between comparison subjects and schizophrenic patients during higher cortical functions.

Objective To observe the clinical effects of chimney technique combined with thoracic endovascular aortic repair(TEVAR) in the treatment of type B aortic dissection.Methods The clinical datas of 56 patients with type B aortic dissection in our hospital were analyzed retrospectively.According to the surgical intervention method,they were divided into observation group (22 cases,TEVAR + chimney technique) and control group (34 cases,TEVAR alone).Surgery related indicators,incidence of postoperative short-term complications and mortality between the two groups were compared.Results The success rates in both groups were 100％,and the surgical time in observation group was longer than that in control group (P ＜ 0.05).There were no significant differences in blood loss,eating time,off-bed time and hospitalization time between the two groups(P ＞ 0.05).The incidence of complications of observation group and control group within 2 weeks after operation were 18.2％ and 5.8 ％ respectively,the difference was not significant(P ＞ 0.05),as well as mortality and incidence of longterm complications during the followed period (P ＞ 0.05).Conclusion The perioperative indexes of single endovascular repair and its combination with chimney technique for the treatment of type B aortic dissection are similar.Chimney technique can reconstruct important branch flows,especially for patients with insufficiency of landing zone.However,particular attention needs to be paid to postoperative complications to avoid negative effect on the prognosis of patients.

OBJECTIVE:To establish a rapid,accurate and standardized DNA barcode identification method for the ferulic me-dicinal plants. METHODS:Genomic DNA was extracted from Ferula sinkiangensis K. M. Shen and Ferula fukanensis K. M. Shen,ITS2 sequences were amplified and sequenced,analytical similarity search method was conducted,13 species 26 samples gene ITS2 sequences of ferulic medicinal plants in GenBank database were downloaded and comparatively analyzed;interspecies and intraspecies genetic distances were calculated,and phylogenetic tree was developed for cluster analysis. RESULTS:According to calculation,species genetic distances of the 15 species ranged 0.009-0.230,average genetic distance was 0.018;results of clus-ter analysis showed DNA barcode ITS2 sequences can cluster the 32 kinds of ferulic medicinal plants into different classes. CON-CLUSIONS:The established DNA barcode ITS2 sequences identification method can accurately and rapidly identify the genuine and false samples of ferulic species.

OBJECTIVE:To establish a method for the content determination of eudesmin in Zanthoxylum simulans. METH-ODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-water(56:44,V/V)at flow rate of 1.0 ml/L,detection wavelength was 221 nm,column temperature was 25 ℃. RESULTS:The linear of eudesmin was 0.208-2.08 μg (r=0.999 8);RSDs of precision,reproducibility and stability tests were lower than 2.0%;recovery was 96.18%-103.04%(RSD=2.58,n=6). CONCLUSIONS:The method is simple and good reproducibility,and can be used for the content determination of eu-desmin in Z. simulans.

Clinical characteristics were retrospectively analyzed in a patient with parathyroid crisis as the main symptoms of parathyroid adenoma and asymptomatic pheochromocytoma.This analysis was aimed to implement specific diagnosis and treatment and to accumulate experience in managing these diseases.

Objective To explore expression of plexinA1 and Ki-67 in tissue of human brain glioma cells and their clinical significance.Methods 43 specimens from patients with brain glioma were collected.Immunohistochemical (IHC) staining was used for detecting the expression of tissue plexinA1 and Ki-67 in human glioma cells of 43 cases of patients with brain glioma.The positive expression rate of plexinA1 and Ki-67 among the different pathological grade tissues and their clinical significance were analyzed.So did correlation studies about plexinA1 and Ki-67.Results The positive expression rates of plexinA1 in Ⅰ-Ⅱ grade group (18 cases) and Ⅲ-Ⅳ grade group (25 cases) were 22.22 ％ (4/18) and 72.00 ％ (18/25) (P ＜ 0.05).The positive expression rates of Ki-67 in Ⅰ-Ⅱ grade group and Ⅲ-Ⅳ grade group were 16.67 ％ (3/18) and 56.00 ％ (14/25),respectively (P ＜ 0.05).PlxinA1 and Ki-67 expression in the tissue of human brain glioma were positively correlated (r =0.997,P ＜ 0.05).Conclusions The positive expression rate of plexinA1 is higher in high malignancy human glioma group than that in low malignancy group which has an important reference value in the estimation of prognosis for human glioma.PlexinA1 and Ki-67 maybe synergism in occurrence and development of glioma.