Objective To explore the application value of high frequency probes in ultrasonic diagnosis of acute appendicitis.Methods Using high frequency probe to detect 356 patients with right lower quadrant abdominal ultrasound,and the results were analyzed.281 cases were confirmed by operation and pathology.Results 240 cases of 356 patients were detected low frequency transducer,and the detection rate was 67.4％.Application of high frequency probe were detected in 322 cases,and the detection rate was 90.4％.Pathological progress of appendicitis with appendicitis,display rates have increased,there was a significant correlation between the two.Conclusion The use of high frequency color ultrasound imaging for suspected acute appendicitis in patients has definite diagnosis and differential diagnosis.

Objective To study the clinical significance of changes of plasma nitric oxide (NO) and tumor necrosis factor (TNF) levels in patients with congestive heart failure (CHF).Methods Plasma NO and TNF in 68 CHF cases were tested.30 healthy subjects served as normal controls.Results Plasma NO and TNF levels in CHF patients were obviously higher than those in normal subjects (P

Objective To investigate the expression of nerve growth factor(NGF)in the adrenaline medullary chromaffin cells(AMCC)and the morphological and functional changes in AMCC of asthmatic rats.Methods The 32 SD rats were randomly divided into two groups:control group(n=8)and asthma group(n=8).Asthma models were established by sensitization and challenge with ovalbumin(OVA).By means of immunohistochemistry(SP)combined with the micro-image analysis to investigate the alterations of NGF immunoreactivity in asthmatic rats and by means of light microscopy and electron microscopy to investigate the ultrastructural changes in AMCC,and detect the concentration of adrenaline and noradrenaline in serum by enzyme-linked- immunosorbent assay(ELISA).Results The positive immunoreactivity was increased in asthmatic AMCC compared with the controls(P

Background and purpose: MicroRNAs are 19–25-nucleotide noncoding RNA molecules that regulate gene expression at the level of transcription and translation. The study aimed to confirm whether miR-216a suppresses cell proliferation and invasion by targeting PRKCA, thus to reveal molecular mechanism that miR-216a functions as a tumor suppressor in glioma. Methods: PRKCA 3’ untranslated region (UTR)-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-216a on luciferase activity. U251 cells were transfected with miR-216a mimics, and next Western blotting was performed to detect the expression of PRKCA protein. The effects of PRKCA downregulation on cell proliferation and invasion were observed after PRKCA siRNA was transfected into U251 cells. U251 cell proliferation assays were performed when cotransfected with miR-216a mimics. Results:The result demonstrated miR-216a could bind to the 3’UTR of PRKCA and inhibited the luciferase activity by 41%. PRKCA protein expressions were significantly down-regulated when miR-216a was overexpressed in U251. siRNA-mediated downregulation of PRKCA could suppress the potentials of cell proliferation and invasion. Conclusion:miR-216a suppresses cell proliferation and invasion by targeting PRKCA in glioma.

Objective:To analyze the characteristic peaks of gypsum fibrosum from authentic product area Hubei Yingcheng via X-ray diffraction. Methods:The fingerprint and common peaks of gypsum fibrosum from different areas were analyzed via X-ray diffrac-tion, and the characteristic peaks of gypsum fibrosum from authentic product area Hubei Yingcheng were identified Results:The finger-prints of X-ray diffraction of gypsum fibrosum from different areas were obtalned. Sixteen common peaks were determined, and two characteristic peaks of gypsum fibrosum from authentic product area Yingcheng were determined via X-ray diffraction with interplanar spacing of 1. 68 and 1. 51, respectively. Conclusion:The determination of two characteristic peaks of gypsum fibrosum from authentic product area Hubei Yingcheng can distinguish gypsum fibrosum from the other areas and provide basis for the quality evaluation of gyp-sum fibrosum.

Objective To evaluate the applicated value of color Doppler ultrasound in diagnosis for neck lymph node metastatic tumor.Methods A retrospective analysis of 53 cases with metastatic tumors in cervical lymph nodes of two-dimensional and color Doppler ultrasonography features was taken out.Results The color Doppler ultrasound on cervical lymph node metastasis tumor location,shape,size and internal structural characteristics were observed,it is found that the characteristic change,provide reliable evidence for the clinical diagnosis.Conclusion The color Doppler ultrasound to cervical lymph node metastatic tumor has a high diagnostic value,can be used as the cervical lymph node metastatic tumor imaging examination of choice.

Objective To observe the clinical effects of chimney technique combined with thoracic endovascular aortic repair(TEVAR) in the treatment of type B aortic dissection.Methods The clinical datas of 56 patients with type B aortic dissection in our hospital were analyzed retrospectively.According to the surgical intervention method,they were divided into observation group (22 cases,TEVAR + chimney technique) and control group (34 cases,TEVAR alone).Surgery related indicators,incidence of postoperative short-term complications and mortality between the two groups were compared.Results The success rates in both groups were 100％,and the surgical time in observation group was longer than that in control group (P ＜ 0.05).There were no significant differences in blood loss,eating time,off-bed time and hospitalization time between the two groups(P ＞ 0.05).The incidence of complications of observation group and control group within 2 weeks after operation were 18.2％ and 5.8 ％ respectively,the difference was not significant(P ＞ 0.05),as well as mortality and incidence of longterm complications during the followed period (P ＞ 0.05).Conclusion The perioperative indexes of single endovascular repair and its combination with chimney technique for the treatment of type B aortic dissection are similar.Chimney technique can reconstruct important branch flows,especially for patients with insufficiency of landing zone.However,particular attention needs to be paid to postoperative complications to avoid negative effect on the prognosis of patients.

Objective To study the anatomical characteristics of the stellate ganglion (SG) for providing morphological data for SG block. Methods The shape, size, position, and relation of 30 cases of SG in 15 adult cadavers were observed and measured. Results Most of the SG was stellate-shaped. The average volume of the stellate ganglion was (0.86?0.07)cm3. The position of the SG could be localized according to the adjacent bone or muscle marks, and the shortest distance was between the SG and the transverse process of the 7th cervical vertebrae. There were many important structures around the SG, in which the vertebral artery was closely related to the SG. Conclusion During performance of the SG block, the surface projection, puncture point, and medication dosage could be judged on the basis of these quantitative anatomic data.

Objective To study the multi-detector spiral CT (MDCT) features of pancreatic tuberculosis to improve the awareness and correct diagnosis. Methods Imaging features of MDCT were retrospectively reviewed on 9 cases with pancreatic tuberculosis that were diagnosed from 2003 to 2009, among them two patients underwent MDCT angiography, the features were compared with those of operative and pathological findings. Results One case of pancreatic cancer, 2 cases of cystadenocarcinoma and 1 case of pseudocyst, 2 cases of pancreatic cancer with retroperitoneal lymph node metastasis, 2 cases of lymphoma were diagnosed by MDCT, and all the lesions were diagnosed as pancreatic tuberculosis pathologically, and the misdiagnosis rate was 100%. Pancreatic tuberculosis often presented as pancreatic head mass, sometimes pancreatic body and tail were involved. MDCT showed isopycnic or low density solid-cyst mass, occasionally calcification was present, with slightly or moderate ring-like enhancement after contrast injection. There was no enlargement of the pancreatic duct. The retroperitoneal and adjacent lymph nodes were always enlarged with ring-like enhancement. Sometimes there was evidence of tuberculosis of other abdominal organs. Conclusions Ring-like enhanced lesions with ring-like enhanced lymph nodes without enlargement of pancreatic duct are characteristic MDCT features of pancreatic tuberculosis.

ObjectiveTo investigate the prognostic factors of non-small cell lung cancer (NSCLC) after resection.MethodsClinical data of 131 NSCLC patients who underwent resection were reviewed and divided into chemotherapy group(86 cases) and non-chemotherapy group(45 cases) according to the treatment method.Survival rate was calculated by Kaplan-Meier method.The prognosis was analyzed by Cox proportional hazards model.ResultsThe median survival time (MST) of squamous cell carcinoma (76 cases),bronchial alveolar cell carcinoma( 8 cases),adenocarcinoma( 35 cases ),adenosquamous carcinoma (12 cases) was 60,54,34,24 months respectively (P＜0.05).For the patients of stage Ⅰ B,the MST of chemotherapy group and non-chemotherapy group was 75 and 76 months respectively(P ＞ 0.05 ).Multivariate analysis showed that tumor size,T stage,N stage,chemotherapy were independent prognostic factors (P =0.080,0.002,0.000,0.029).Conclusions Squamous cell carcinoma and bronchial alveolar cell carcinoma have better prognosis than adenocarcinoma,adenosquamous carcinoma.For the patients of stage Ⅰ B,the survival time can't be prolonged through platinum-based chemotherapy.Tumor size,T stage,N stage,chemotherapy are independent prognostic factors.