OBJECTIVE:To establish an HPLC method to determine the content of main component in idrocilamide ointment. METHODS: The samples were separated on HP-ODS column with column temperature maintained at room temperature. The mobile phase consisted of methanol-water (65∶35) with a flow rate of 1.0 mL?min-1. The detection wavelength was set at 276 nm and the injection volume was 20 ?L. RESULTS: The linear range of idrocilamide was 0.05～4 ?g?mL-1,the average recovery rate was 99.97% (RSD=0.80%). CONCLUSION: This method is sensitive,accurate and applicable for the determination of the content of main component in idrocilamide ointment.

Objective To analyze the clinical characteristics and drug resistance of gram -negative bacillus in Department of Respiratory Medicine.Methods 100 patients in Department of Respiratory Medicine were selected, analyzed the clinical characteristic and drug resistance of 125 strains of gram -negative bacillus that was separated. Results 125 strains of pathogenic gram negative bacilli were isolated out of 100 patients,among which 34 strains were pneumonia klebsiella bacteria,24 strains were acinetobacter baumannii,18 strains were pseudomonas aeruginosa, 10 strains were enterobacter cloacae,18 strains were escherichia coli and 21 strains were other bacteria.Results The drug resistance rates were 79.4%,76.5%,76.5% and 76.5% respectively in klebsiella pneumoniae to amikacin, cefotaxime,ampicillin and imipenem,was more sensitive to meropenem and cefoperazone,the drug resistance rate was 100.0% in acinetobacter baumannii to ampicillin and cefazolin,and was 91.7% to ceftriaxone,and the resistance rate for other drugs are higher generally.The drug resistance rate was 100.0% in pseudomonas aeruginosa to ampicillin and ceftriaxone,and was 94.4% to cefotaxime and levofloxacin,the drug resistance rate was 90.0% in enterobacter cloacae to ampicillin,and was 60.0% to ceftriaxone,cefazolin and cefoxitin,the drug resistance rate on the other drug was relatively low.Conclusion Klebsiella pneumoniae,acinetobacter baumanni and pseudomonas aeruginosa were the main gram negative bacillis the cause hospitalized patients with drug resistance.

0 05) when E/T was 1, whereas DNA fragmentation in oligonucleotide treated cells was significantly higher as compared with non treated cells ( P

Objective To study the significance of sentinel lymph node biopsy (SLND) in clinical stage Ⅰ,Ⅱ breast cancer patients. MethodsFrom January 1999 to January 2000, 126 patients with clinically stage Ⅰ,Ⅱ breast cancer received SLND guided by methylenc blue staining, and axillary lymph nodes clearance. Multilevel section and Immunohistochemistry (IHC) were performed in lymph nodes of patients with negative nodes. Results SN was identified in 95/126 cases(75.4%). The sensitivity of SLND was 91.7%, the specificity was 100%. It had 100% positive predictability and 92.5% negative predictability. The accuracy rate of intraoperative frozen-section examination of SN was 93.4%. One of 35 patients (2.9%) with H&E negative nodes had evidence of lymph node metastasis in the SN by multilevel sectioning and IHC.ConclusionSLND is extremely accurate in prediction of the lymph node status in patients with breast cancer. Multilevel sectioning and IHC analysis of SN improves the sensitivity of this staging technique.

OBJECTIVE:To compare the clinical efficacy and safety of salmeterol-fluticasone alone versus fluticasone propio-nate combined with montelukast sodium in the treatment of children with moderate persistent asthma. METHODS:275 children with moderate persistent asthma were randomly divided into inhaled corticosteroids(ICS)+ long acting β2 receptor agonist(LABA) group (139 cases) and ICS+ leukotriene receptor antagonists (LTRA) group (136 cases). ICS+LABA group was given Salmeter-ol-fluticasone powder inhalant,1 inhalation,bid;ICS+LTRA was given Fluticasone propionate inhalation aerosol,bid+Montelu-kast sodium chewable tablet 5 mg,before bedtime,qd. Both groups received more than 12 weeks of treatment. Body indexes of 2 groups were observed after treatment,such as asthma control degree,asthma score,the percentage of peak expiratory flow(PEF) in estimated value,aberration rate of PEF,the levels of eosinophi cationic protein(ECP)and leukotrienes E4(LTE4)in peripheral blood,the times of taking short acting β2 receptor agonist (SABA) during treatment,asymptomatic days,compliance,the inci-dence of ADR,etc. RESULTS:Total effective rate of ICS+LABA group(86.33%)after 4 weeks treatment was higher than that of ICS+LTRA group(58.09%),with statistical significance(P<0.05);that of ICS+LABA(99.28%)after 12 weeks treatment was higher than that of ICS+LTRA group (98.53%),without statistical significance (P>0.05). The day and night asthma score of 2 groups decreased significantly and the percentage of PEF in estimated value increased significantly after treatment,there was statisti-cal significance compared to before treatment(P<0.05). There was no statistical significance in above indexes between 2 groups af-ter treatment(P>0.05). The levels of ECP and LTE4 decreased significantly in 2 groups after treatment,there was statistical signif-icance compared to before treatment(P<0.05);those of ICS+LTRA group decreased significantly compared to ICS+LABA group, there was statistical significance between 2 groups(P<0.05). There was no statistical significance in the aberration rate of PEF af-ter treatment,the times of taking SABA,asymptomatic days,compliance and the incidence of ADR between 2 groups during treat-ment(P>0.05). CONCLUSIONS:Salmeterol-fluticasone alone versus fluticasone propionate combined with montelukast sodi-um in the treatment of children with moderate persistent asth-ma both have good therapeutic efficacy and safety,while thelatter one has stronger inhibition effect on ECP and LTE4,and to control asthma symptoms more rapidly.

Objective To observe the level of serum transforming growth factor ?(TGF ?) in patients with colorectal cancer and study its clinical significance. Methods Serum TGF ? was measured with radioimmunoassay in 54 patients with colorectal cancer and 25 healthy subjects,compared with 42 patients with colorectal cancer before and after the operation. Results The levels of serum TGF ? were significantly higher in patients with colorectal cancer than those in healthy controls(P

Objective To know the influence of out-hospital nursing with no gap on the level of HbAlc for patients with diabetes.Methods Divided 80 patients with diabetes into the intervention group and control group randomly,there were 40 cases in the each group.Out-hospital nursing cares with no gap were used in the intervention group,while the routine nursing cares were used in the control group.Fellowed up 1 year.Compared the FPG,2 hPG and HbAlc between the two groups at the time points of the 3rd,6th,9th and 12th month when out of hospital.Results All the indexes which had monitored were better in the intervention group than those of in control group.Conclusions Out-hospital nursing with no gap can remain the indexes in the normal level,and then promote their quality of life,which should be developed in clinical field.

Objective:To discuss the effect and significance of reasonable medication review mode for intravenous medical advice on the platform of pharmacy intravenous admixture service( PIVAS) . Methods:The effect of prescription review interference in intrave-nous drugs on the platform of PIVAS was studied through analyzing irrational prescriptions before and after the performance of PIVAS for 25 departments in our hospital. Results:The real-time review and interference procedure for intravenous transfusion was established, which could significantly increase the percentage of reviewed medical advice and the correction rate of irrational medical advice, and re-duce the percentage of irrational medical advice. The medical advice review quality was also increased, which could guarantee the med-ication safety in patients. Conclusion:The real-time review and interference procedure for intravenous transfusion on the platform of PIVAS can significantly improve the rational medication level of intravenous drugs and avoid the hidden danger of irrational medical ad-vice to the maximum extent to ensure the medication safety in patients, which is also beneficial to the provision of medication service and new platform establishment for the development of clinical pharmacy in hospitals.

Objective To probe into the clinical application value of dynamic contrast-enhanced MR imaging (DCE-MRI) and diffusion weighted imaging (DWI) in identifying benign and malignant breast lesions.Methods 60 patients with clinically suspected breast lesions underwent MR examination.Morphologic features,early enhancement rate,time-signal intensity curve (TIC),apparent diffusion coefficient (ADC) value,as well as the differences between ADC values of the surrounding normal tissues and these of the lesions were compared and analyzed.Results The areas under the receiver operating characteristic curves (AUC) of the early enhancement rate,TIC,ADC value,ADC difference value were 0.741,0.808,0.882 and 0.959,respectively;the best diagnostic thresholds for the early enhancement rate,ADC value,and ADC difference value were 163％,1.30× 10-3 mm2/s and 0.47× 10-3 mm-2/s,respectively.The sensitivity of morphologic features,early enhancement rate,type Ⅲ curve,type Ⅱ and type Ⅲ curve,ADC value and ADC difference value in differentiating benign and malignant breast lesions was 53.1％,59.4％,43.8％,90.6％,93.8％ and 96.9％,respectively;the specificity was 85.7％,82.1％,89.3％,57.1％,75.0％ and 82.1％,respectively;the positive predictive values were 81.0％,79.2％,82.4％,70.7％,81.1％ and 86.1％,respectively;the negative predictive values were 61.5％,63.9％,58.1％,84.2％,91.3％ and 95.8％,respectively;and the accuracy was 68.3％,70.0％,65.0％,75.0％,85.0％,90.0％,respectively.Conclusion DCDMRI and DWI play important roles in differential diagnosis of benign and malignant breast lesions,among which ADC difference value is associated with the highest diagnostic capability.Combination of multiple imaging diagnostic methods is necessary to improve the diagnostic accuracy of breast lesions.

OBJECTIVE:To establish a method for the content determination of paeoniflorin in Bazhen pill(concentrated pill). METHODS:HPLC was performed on the column of wondasil C18 with mobile phase of acetonitrile-1% phosphoric acid(15∶85, V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 230 nm,and column temperature was 30 ℃. RESULTS:The lin-ear range of paeoniflorin was 0219-1.32 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 95.82%-101.82%(RSD=2.13%,n=6). CONCLUSIONS:The method is simple,stable and reproducible, and can be used for the content determination of Bazhen pill(concentrated pill).