Objective To study the regulation of dry substance and active components accumulation in the fruit of Forsythia suspensa. Methods The dry substance of the fruit was analyzed by the method of hundred grain mass. The contents of the active components in leaves, shucks, and seeds were analyzed by HPLC. Results The period for F. suspensa to accumulate the dry substance was mainly May and July. During the whole growth phase, the active components, such as forsythiaside, forsythin, and rutin in different parts, were decreasing. From the last ten-day of August to the first ten-day of September, the accumulation of the dry substance came to stability and the contents of active components in shucks and seed were stable. The contents of active components in shucks decreased rapidly from September to October, and then came to stability and the contents in shucks is the least during the last ten-day of October. So the contents of active components in younger F. suspensa are higher than that in elder one, and the proportion among forsythiaside, forsythins, and lution is also significantly different from elder seeds. Conclusion It is more preferable to pick younger F. suspensa from the last ten-day of August to the first ten-day of September and to pick elder F. suspensa during last ten-day of October.

The article gives a brief account of the application of clinical observation to the evaluation of medical students' core competence. It expounds the key points in the assessment about their professional values,attitudes and behavior, emphasizing the application scope, working principle and advantages and disadvantages in the rank evaluation as well as the methods to enhance the effectiveness of evaluation.

Objective To observe the clinical effect of using single epidural injection of S-Ropivacaine Mesylate to ease the pain after gynaecology and obstetrics operations.Methods 180 patients who need gynaecological operations were randomly divided into two groups.The group of therapy was injected the analgesic(ropivacaine + morphia)at once when suturing the skin.And the comparing group only injected 5～6ml of analgesic at the stage of skin suture,then selfoperated analgesia pump will be used.The changes of hemodynamics and SpO_2 pf the two groups,the analgesic activities after 48 hours,occurrence of adverse reaction,etc were analyzed.Results The differences of MAP,SpO_2,and HR had statistical significance(P＞0.05);After the surgery,the effept of analgesic of 24h and 48h grade 0(pain-free rate)in therapy group is much more obvious than comparing group(χ~2=3.974,4.031,P＜0.05);Neither group has severe adverse reaction.Conclusion The effect of using sihgle epidural injection of S-Ropivacaine Mesylate to ease the pain after gynaecology and obstetrics surgeries is gopd,and has little adverse reaction.

Objetive: To observe the reversal effects of tumor necrosis factor (TNF) ? and interferon (IFN) ? on multidrug resistance (MDR) in K562 cell line resistant to adriamycin (ADR) (K562/A02). Methods: After treatment with TNF-? and IFN-? respectively, K562/A02 sensitivity to ADR was investigated using tetrazolium dye assay. MDR1 gene expression was assayed by semiquantitative reverse transcription-polymerase chain reaction and immunocytochemistry staining. Intracellular ADR concentration was also observed with flow cytometry. Results: The reversal activity after treatment with TNF-? or IFN-? was found to be increased up to 6 and 5-fold respectively at 24 h, and the peak with the increase of 10 and 8-fold respectively was seen at the 48 h (both P

ve To study the way of oxidative stress induced by soot rat pulmonary cells type Ⅱ ?Methods Before exposure to soot extracts, the rat pulmonary cells were pretreated with anti-oxidative agent, N-acetylcysteine (NAC), vitamin C, vitamin E and mannitol for 30 minutes, then the ceils were exposed to the soot extracts for 24 hours. MTT assay was applied to detect the cytotoxicity. Results It showed that NAC, vitamin C and vitamin E could reduce the cytotoxicity of rat pulmonary cell caused by soot, but no same change was observed in that of man-nitol. Conclusion It was suggested that the DNA oxidative stress of soot may be caused by multi-ways, such as in-duction of oxygen free radical and lipid peroxide etc.

0.05).The decrease of Mel-18 expression was signifi cantly negatively correlated with lymph node metastasis and clinical stage(P

Phloroglucinol is an antineoplastic effective compound from Dryopteria Crassirhizoma Nakai. In this study, mice implanted with leukemia P388 were examined under an electron microscope. It' was found that the leukemia bearing mice, which had been injected with phloroglucinol exhibited degeneration and swelling of mitochondria in the P38'8 cells. Measurement with an oxygen electrode showed that the drug treatment of P388 cells markedly lowered their oxygen consumption in comparison with the control cells .The results demonstrate that phloroglucinol inhibits respiration of tumor cells. Probably, the mitochondria of tumor cells is a target that the drug attacks.

Background and purpose: Mel-18 is one of the mammalian polycomb group members. A number of related researches have implied that Mel-18 may play a role in human tumorigenesis.In this study, we measured the expression of Mel-18 in gastric carcinoma cells in vivo to explore the expression and clinical significance of Mel-18 in gastric carcinoma. Methods: Real time RT-PCR was used to detect the expression of Mel-18 in cancer tissue and corresponding normal tissue in 52 cases of gastric carcinoma. The association between Mel-18 expression and the clinicopathological parameters of the tumors was analyzed. Results: The analysis revealed that there was significantly decreased expression of Mel-18 in 18 (34.62%)carcinoma tissues in comparison with para-cancer normal tissue. There was no correlation between Mel-18 expression and clinicopathological parameters, such as age, gender, tumor size and histological differentiation (P>0.05).The decrease of Mel-18 expression was significantly negatively correlated with lymph node metastasis and clinical stage (P<0.05). The expression levels of Mel-18 evaluated by the ratios of gene expression were 1.357,0.453,0.183 and 0.170 in stage Ⅰ, Ⅱ,Ⅲ and Ⅳ gastric carcinoma, respectively. They were 0.634 and 0.210 in patients without lymph node metastasis and in patients with lymph node metastasis, respectively. Expression of Mel-18,lymph node metastasis, and clinical stage were significant covariates, respectively (P<0.05). Conclusion: Our results showed that Mel-18 might play a crucial role in tumorigenesis and progression of gastric carcinoma. It is possible that Mel-18 could be used as one of the biomarkers for predicting the prognosis of gastric carcinoma.

Objective To estimate the value of neck circumference in diagnosis of metabolic syndrome. Methods We analyzed the neck circumference of 523 volunteers and the occurrence of metabolic syndrome among them. Then we updated and revalued their data after follow-up for 1.5 years, meanwhile, we evaulated the value of Body Mass Index (BMI) and neck circumference in predicting metabolic syndrome. Results The increase of neck circumference was closely related to the occurrence of metabolic syndrome , and this tendency continued in the follow-up. Compared with BMI , neck circumference was sensitive in identifying the patients of metabolic syndrome. Conclusion Neck circumference has great value in diagnosis and treatment of metabolic syndrome.

In this research, Casuarina eguisetifolia Linn was used to verify the broadly suitability of DNA bar-codes in identification of Li-medicine plants and systematic development of species. The genomic DNA of 22 samples collected C. eguisetifolia and its adulterants were amplified by 4 pairs of primers respectively (ITS (inter-nal transcribed spacer), ITS2 (internal transcribed spacer 2), trnH-psbA , rbcL) and sequenced bi-directionally. Obtained sequences were assembled using CodonCode Aligner. The dates were analysised using MEGA5.1 in ac-cordance with the kimura 2-parameter (K2P) model. The neighbor-joining (NJ) phylogenetic trees were construct-ed. Our study demonstrated the efficacy of ITS/ITS2 to distinguish between C. eguisetifolia and other adulterants species at the molecular level. Comparative to the primer of trnH-psbA and rbcL, there was a obviously DNA gap. The NJ trees showed that the several species of Casuarina can be classified to same types to show a obvi-ously monophyly, which the nearest family was Guttiferae. Therefore, ITS/ITS2 regions can accurately distinguish the original plant of Li-medicine. The systematic evolution of Casuarina can be verified in the molecular level.