Objective To study dynamic response of serum lipid after fat meal in old persons with fasting TG≤1 70 mmol/L. Methods Using fat meal of 63 9 g/m 2 body surface area, TG, TC, HDL C and LDL C of both elderly and control groups in fasting status and 2, 4, 6 and 8 h after fat meal were determined. Results The TG value after fat meal was significantly higher than that in fasting status( P 0.05). Conclusions The expurgation of triglycerides is delayed in elderly people, meaning that the capacity for fat removal is decreased, and it is possibly the main cause of TG increase in the elderly.

Objective To explore the performance of meningiomas in MRI dynamic enhancement and DWI sequence,and to improve the accuracy of differential diagnosis of benign and malignant meningiomas.Methods Meningioma MRI data,which were pathology proved by the Guilin medical college affiliated hospital at different levels(Ⅰ,Ⅱ and Ⅲ),were retrospectively analyzed.The enhancement of meningiomas at all levels and the extent of edema were summarized,at the meantime the dynamic enhancement perfusion parameters and ADC values of meningioma MRI meningioma were contrastively analyzed.Results There were 30 cases of meningioma located in the brain falcus,27 cases of meningioma located in the sagittal sinus,10 cases of meningioma located in the cerebellopontine angle and 6 cases of meningioma located in other parts.The difference of ADC value between grade Ⅰ meningiomas and grade Ⅲ meningiomas was statistically significant different[(1.253±0.123)×10-3 mm2/s vs.(0.891±0.103)×10-3 mm2/s,P<0.05].The relative blood flow(rCBF)and relative blood volume(rCBV)of grade Ⅰ meningiomas were significantly lower than those of grade Ⅲ meningiomas(P<0.05).Conclusion MRI dynamic enhancement combined with DWI can accurately identify grade Ⅰ meningiomas and grade Ⅲ meningiomas,and it is necessary that the assessment of its classification should be appropriately considered to the higher level for patients who do not have a characteristic manifestation of meningioma in the preoperative.

Dynamic color is an important carrier that takes information in some special occupations. However, up to the present, there are no available and objective tests to evaluate dynamic color processing. To investigate the characteristics of dynamic color processing, we adopted two patterns of visual stimulus called "onset-offset" which reflected static color stimuli and "sustained moving" without abrupt mode which reflected dynamic color stimuli to evoke event-related brain potentials (ERPs) in primary color amblyopia patients (abnormal group) and subjects with normal color recognition ability (normal group). ERPs were recorded by Neuroscan system. The results showed that in the normal group, ERPs in response to the dynamic red stimulus showed frontal positive amplitudes with a latency of about 180 ms, a negative peak at about 240 ms and a peak latency of the late positive potential (LPP) in a time window between 290 and 580 ms. In the abnormal group, ERPs in response to the dynamic red stimulus were fully lost and characterized by vanished amplitudes between 0 and 800 ms. No significant difference was noted in ERPs in response to the dynamic green and blue stimulus between the two groups (P>0.05). ERPs of the two groups in response to the static red, green and blue stimulus were not much different, showing a transient negative peak at about 170 ms and a peak latency of LPP in a time window between 350 and 650 ms. Our results first revealed that some subjects who were not identified as color blindness under static color recognition could not completely apperceive a sort of dynamic red stimulus by ERPs, which was called "dynamic red blindness". Furthermore, these results also indicated that low-frequency ERPs induced by "sustained moving" may be a good and new method to test dynamic color perception competence.

Objective To investigate the inhibitory effect of curcumin on the telomerase activity of HeLa cells.Methods The growth inhibition rate of HeLa cells treated with curcumin was measured with MTT method.The expression of hTERT mRNA was studied using RT-PCR.Telomerse activity was examined by TRAP-ELISA.Results The growth of HeLa cells treated with curcumin was inhibited in concentration dependent manner and the expression of hTERT mRNA and the level of telomerase activity were down-regulated significantly.Conclusion Curcumin can inhibit telomerase activity of HeLa cells.The underlying mechanism might be related to the down-regulation of hTERT transcription.

Objective To study the application of immunofixation electrophoresis(IFE) in typing M proteins. Methods Serum M proteins were detected in 43 patients and 20 normal controls by agarose gel IFE and rate nephelometery. Results Of 43 patients, 29 were affirmed to have M proteins, including 20 cases of IgG ?, 5 IgG ?, 2 IgM ?, 1 IgA ? and 1 ?. Conclusion The technology of IFE, characterized by easy differentiation of electrophoretic bands, simple operation and rapidity, has great value in typing M proteins.

Objective To investigate the cytokine spectrum of cultured human amniotic-derived mesenchymal stem cells (A-MSC) for understanding its basis of molecular biology in hematopoietic in vitro.Methods Their hematopoietic cytokines expression was analyzed using RT-PCR in the mRNA level. Results It showed that in vitro subcultured human amniotic-derived mesenchymal stem cells were capable to express many important hematopoietic cytokines such as LIF, SCF, M-CSF, G-CSF, GM-CSF, IL-3, IL-6, IL-11 and so on. Conclusion Production of abundant of hematopoietic cytokines by human amniotic-derived mesenchymal stem cells may be effective for hematopoietic support and HSC transplantation.

Objective To investigate the optimal b value of diffusion-weighted imaging (DWI)in predicting the grade of cerebral gliomas.Methods 38 patients with pathologically-proved brain gliomas (24 high-grade gliomas and 14 low-grade ones)were studied retrospectively.All patients received conventional MRI and DWI examination with three different b values (1 000 s/mm2 ,2 000 s/mm2 and 3 000 s /mm2 ).The signal features of the tumor on DWI with three different b values were evaluated and compared.The minimum ADC values of the tumors were calculated and compared between each other.Through drawing the ROC curve of different b values, the best diagnostic threshold was found.The sensitivity and specificity in predicting the grade of brain gliomas were assessed using Chi-square test.Results On DWI with b value of 3 000 s/mm2 ,91.6% (22/24)of high-grade tumors showed hyper-intensity, while 85.7%(12/14)of low-grade tumors presented hypo-intensity signals.Regarding hyper-intensity signals as diagnostic criterion for high-grade tumors,the sensitivity and specificity were 91.6% and 100% respectively.When the b value was similar,the minimum ADC value of high-grade glioma was significantly lower than that of low-grade glioma,and statistically significant differences in differentiating high-grade glioma from low-grade glioma existed among three b values (P <0.05).When the ADC 3 000 value<0.74×10 -3 mm2/s was regarded as a standard for the identification of high-grade and low-grade glioma,the sensitivity and specificity were 100% and 87.3%respectively.Conclusion DWI with high b value is more useful than standard moderate b value in preoperative grading the gliomas.When DWI with b value of 3 000 s/mm2 is used,the minimum ADC value will provide quantitative indicators in preoperatively precisely predicting grading glioma.

0.05). However,the expression level of TKTL1 gene was significantly downregulated in the CNE cells transfected with siRNA TKTL1 construct compared with the cells transfected with control vector. Cancer cells were arrested in G0/G1 phase,and cancer cell proliferation was significantly inhibited in the CNE cells transfected with siRNA TKTL1 construct. CONCLUSION:TKTL1 plays an important role in the total transketolase activity and cell proliferation of human nasopharyngeal carcinoma. TKTL1 may be considered as a potential target for novel anti-cancer therapy.

Objective To explore the outcome of small external fixation devices combined with flap for fracture and dislocation of articulationes interphalangeaes.Methods Twelve cases (13 fingers) with fracture and dislocation of articulationes interphalangeae were treated with small external fixation devices combined with flap.Blood vessel grafting and crossing anastomosis were performed.The area of the flap was 1.0 cm ×1.5 cm -2.5 cm × 3.0 cm.Results Follow-ups lasting from 6 months to 2 years showed that all fingers and flaps were survived postoperatively.Fixation average time of small external fixation was 37 days.Fracture healing was found in all cases with satisfactory effect and with better apperance.Conclusion Small external fixation devices combined with flap was proper for fracture and dislocation of articulationes interphalangeae.

Objective:To investigate the effects of slowly release(SR) carbohydrate system on glucose and lipide metabolism in the elder patients with type 2 diabetes. Methods:The previous enteral nutrition was replaced totally or partially with Glucerna in 11 cases of elder patients with type 2 diabetes for 1-3 months.The changes in hemoglobin, lymphocyte counts, serum albumin, total protein, glycemia, postprandial 2 hours blood glucose,glycosylated hemoglobin, triglyceride, cholesterol, kidney function,electrolytes and the symptoms of gastrointestinal tract were observed. Result:Total protein(P